LOCUS       MR211709                8325 bp    DNA     circular  SYN 
DEFINITION  Tsc1 (Myc-DDK-tagged) - Mouse tuberous sclerosis 1 (cDNA clone MGC:63425 IMAGE:5720967)
ACCESSION   BC052399
VERSION     BC052399.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4482..4573 
                     /label="Myc-DDK"  
     misc_feature    4603..5188 
                     /label="PolyA signal"  
     misc_feature    4660..4679 
                     /label="Restriction Enzyme"  
     misc_feature    5464..6136 
                     /label="ColE1"  
     misc_feature    6566..7360 
                     /label="Kan/Neo"  
     misc_feature    7395..7697 
                     /label="SV40 ori"  
     misc_feature    7783..8260 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4464..4470 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4464 
                     /label="Tsc1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCCCAGTTA GCCAACATTG GGGAGCTGCT CTCCATGCTG GACTCCTCCA 
     1081 CACTGGGTGT GCGGGATGAC GTGACAGCCA TCTTCAAGGA GTCCCTCAAT TCTGAACGTG 
     1141 GGCCTATGCT TGTCAACACG TTGGTTGATT ATTACCTGGA AACCAATTCT CAGCCGGTAT 
     1201 TGCACATCCT GACCACCTTG CAGGAGCCAC ACGATAAGCA CCTCTTGGAC AAAATTAATG 
     1261 AGTATGTAGG CAAAGCTGCT ACCCGTTTAT CCATCCTCTC GCTGCTGGGG CATGTTGTGA 
     1321 GACTGCAGCC ATCTTGGAAG CATAAGCTCT CTCAAGCACC TCTTCTGCCT TCTTTATTGA 
     1381 AATGTCTCAA GATGGACACT GATGTTGTGG TCCTCACAAC TGGTGTCTTG GTGTTGATCA 
     1441 CCATGCTCCC GATGATCCCG CAGTCAGGGA AGCAGCACCT TCTCGACTTC TTTGACATCT 
     1501 TTGGCCGTCT CTCGTCATGG TGCCTGAAGA AACCAGGCCA TGTGACAGAA GTGTACCTGG 
     1561 TCCATCTTCA TGCCAGTGTT TATGCCCTCT TTCACCGCCT TTATGGGATG TACCCATGTA 
     1621 ACTTCGTCTC CTTCCTGCGC TCTCACTACA GTATGAAGGA AAACGTGGAG ACTTTTGAAG 
     1681 AAGTGGTCAA GCCAATGATG GAGCATGTGC GAATTCACCC GGAATTAGTG ACTGGATCCA 
     1741 AGGACCATGA ACTGGACCCT CGAAGGTGGA AGACATTAGA AACTCATGAT GTTGTAATAG 
     1801 AGTGTGCCAA AATCTCTCTG GACCCTACAG AAGCCTCGTA TGAAGATGGC TATTCTGTGT 
     1861 CACACCAGCT CTCTGCTTGC TTCCCTTACC GTTCAGCTGA TGTCACCACC AGCCCTTATG 
     1921 TGGACACACA GAATAGCTAT GGGGGTTCTA CTTCCACCCC TTCCTCCAGC TCTCGGCTGA 
     1981 TGTTGTTCAG TCCACCTGGG CAGCTACCTC AGAGTTTGAG TTCCCCATCA ACACGGCTGT 
     2041 TACCTGAGCC GCTGCAAGCT AGTCTCTGGA GCCCATCTGC GGTCTGTGGT ATGACCACTC 
     2101 CTCCTACGTC TCCTGGAAAT GTCCCAGCTG ATTTGTCACA TCCGTATAGT AAAGCCTTTG 
     2161 GTACCACTGG TGGAAAAGGA ACTCCTTCAG GAACCCCAGC GACCTCTCCT CCTCCAGCCC 
     2221 CACCTTGTCC CCAAGATGAC TGTGTGCATG GTTCAGCAGC CCAGGCCTCA GCCACAGCCC 
     2281 CCAGGAAGGA AGAAAGAGCA GATTCCTCAA GGCCTTACCT TCACAGACAG TCAAACGACC 
     2341 GAGGATTAGA GGATCCACCT GGAAGCAAAG GTTCCGTTAC TCTGAGGAAT CTACCTGATT 
     2401 TCCTAGGTGA TCTGGCTTCT GAGGAAGACA GTATCGAGAA AGATAAGGAA GAAGCTGCAA 
     2461 TATCTAAAGA GCTTTCTGAG ATCACTACTG CAGAGGCGGA TCCTGTAGTT CCTCGAGGGG 
     2521 GCTTTGACTC TCCCTTCTAC CGAGACAGTC TCTCTGGCTC TCAGCGGAAG ACTCATTCGG 
     2581 CAGCCTCTGG GACTCAGGGC TCCAGCGTGA ACCCTGAGCC TTTGCACTCC TCCCTGGACA 
     2641 AACATGGGCC TGACACACCA AAGCAAGCCT TTACTCCCAT AGACCCACCC TCTGGCAGTG 
     2701 CTGATGTCAG TCCCGCTGGG GACAGGGATC GCCAGACTTC TCTGGAGACC AGTATCCTCA 
     2761 CTCCCAGCCC TTGCAAAATC CCACCTCAGA GGGGAGTGAG CTTTGGAAGT GGGCAGCTTC 
     2821 CCCCATATGA TCATCTCTTT GAGGTGGCCT TGCCAAAGAC TGCCTGTCAC TTTGTCAGCA 
     2881 AGAAGACTGA GGAGCTGTTG AAGAAAGTGA AAGGAAACCC TGAGGAAGAC TGTGTGCCCT 
     2941 CTACCTCCCC AATGGAAGTA CTGGACAGAC TGATAGAGCA GGGAGCAGGT GCGCACAGCA 
     3001 AGGAGCTGAG CAGGTTGTCC CTGCCCAGCA AGTCTGTTGA CTGGACCCAC TTTGGAGGCT 
     3061 CTCCTCCCTC AGATGAGCTC CGGACCCTCC GAGACCAGTT GCTTTTACTG CACAATCAGC 
     3121 TGCTGTACGA GCGCTTCAAG CGGCAGCAGC ATGCACTCAG GAACAGAAGG CTCCTGCGCA 
     3181 AGGTGATCCG AGCAGCGGCT CTGGAGGAAC ACAATGCAGC AATGAAAGAT CAGTTGAAGT 
     3241 TACAAGAGAA GGACATACAG ATGTGGAAGG TGAGTCTGCA GAAAGAACAA GCCCGATACA 
     3301 GTCAGCTTCA GGAACAACGT GACACCATGG TGACCCAACT GCATAGCCAG ATCAGACAGC 
     3361 TACAGCATGA CCGAGAAGAA TTCTACAACC AGAGTCAGGA GTTACAGACA AAGCTGGAGG 
     3421 ACTGCAGAAA CATGATTGCG GAGCTTCGGG TGGAGCTGAA GAAGGCTAAC AACAAGGTGT 
     3481 GCCACACTGA GCTGCTGCTC AGCCAGGTCT CTCAGAAGCT CTCCAACAGT GAGTCAGTGC 
     3541 AGCAGCAGAT GGAGTTCTTG AATAGGCAGC TCCTGGTGCT CGGGGAGGTC AATGAGCTGT 
     3601 ACCTGGAGCA GCTGCAGAGC AAGCATCCTG ACACCACCAA GGAAGTAGAA ATGATGAAAA 
     3661 CTGCATATCG GAAAGAGCTA GAGAAAAACA GAAGCCACCT TCTCCAGCAG AACCAAAGGT 
     3721 TGGACGCCTC ACAGAGGCGA GTTTTGGAAC TGGAGTCTCT TCTGGCCAAG AAAGACCACC 
     3781 TTCTCCTAGA ACAGAAGAAA TATCTTGAGG ATGTCAAGAG CCAGGCGAGT GGACAGCTGC 
     3841 TGGCTGCAGA GAGCAGGTAT GAGGCTCAGA GAAAGATCAC CCGGGTGTTG GAACTGGAGA 
     3901 TCCTAGACTT GTATGGCAGG TTGGAAAAAG ATGGCCGCCT ACGGAAACTA GAAGAGGACA 
     3961 GAGCAGAGGC AGCAGAGGCA GCAGAAGAGA GGCTTGACTG TTGTAGTGAT GGATGCACAG 
     4021 ATTCCTTGGT AGGACATAAT GAAGAGGCTT CTGGTCACAA TGGTGAGACC AGGACCTCCA 
     4081 GACCTGGTGG CACCCGGGCC AGCTGTGGAG GTAGAGTCAC TGGAGGCAGC AGCAGCAGCA 
     4141 GCAGTGAGCT TTCCACTCCA GAGAAACCCC CGAGCCAGAG GTTCAGCAGC ATCCCCACCA 
     4201 CTGTTGGCTC ACTTCCCAGT TCCAAAAGCT TCCTGGGCAT GAAGGCCCGG GAGCTGTTCC 
     4261 GTAATAAGAG CGAGAGCCAG TGTGATGAGG ACAGCGTGAC CATGAGTAGC AGCAGCCTTT 
     4321 CTGAGACCCT GAAGACAGAA CTGGGCAAGG ACTCGGGCAC AGAAAACAAG ACTTCCCTGA 
     4381 GTCTAGATGC CCCACACCCA TCTTCCCCAA ACTCAGACAA TGTGGGGCAG CTCCACATCA 
     4441 TGGACTACAA TGAGACTCAT CCTGAACACA GCACGCGTAC GCGGCCGCTC GAGCAGAAAC 
     4501 TCATCTCAGA AGAGGATCTG GCAGCAAATG ATATCCTGGA TTACAAGGAT GACGACGATA 
     4561 AGGTTTAAAC GGCCGGCCGC GGTCATAGCT GTTTCCTGAA CAGATCCCGG GTGGCATCCC 
     4621 TGTGACCCCT CCCCAGTGCC TCTCCTGGCC CTGGAAGTTG CCACTCCAGT GCCCACCAGC 
     4681 CTTGTCCTAA TAAAATTAAG TTGCATCATT TTGTCTGACT AGGTGTCCTT CTATAATATT 
     4741 ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG GGGCAAGTTG GGAAGACAAC CTGTAGGGCC 
     4801 TGCGGGGTCT ATTGGGAACC AAGCTGGAGT GCAGTGGCAC AATCTTGGCT CACTGCAATC 
     4861 TCCGCCTCCT GGGTTCAAGC GATTCTCCTG CCTCAGCCTC CCGAGTTGTT GGGATTCCAG 
     4921 GCATGCATGA CCAGGCTCAG CTAATTTTTG TTTTTTTGGT AGAGACGGGG TTTCACCATA 
     4981 TTGGCCAGGC TGGTCTCCAA CTCCTAATCT CAGGTGATCT ACCCACCTTG GCCTCCCAAA 
     5041 TTGCTGGGAT TACAGGCGTG AACCACTGCT CCCTTCCCTG TCCTTCTGAT TTTAAAATAA 
     5101 CTATACCAGC AGGAGGACGT CCAGACACAG CATAGGCTAC CTGGCCATGC CCAACCGGTG 
     5161 GGACATTTGA GTTGCTTGCT TGGCACTGTC CTCTCATGCG TTGGGTCCAC TCAGTAGATG 
     5221 CCTGTTGAAT TGGGTACGCG GCCAGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT 
     5281 ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA 
     5341 AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC 
     5401 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA 
     5461 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC 
     5521 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC 
     5581 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA 
     5641 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC 
     5701 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG 
     5761 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG 
     5821 AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG 
     5881 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA 
     5941 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA 
     6001 CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT 
     6061 CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA 
     6121 GTAACCTGAG GCTATGGCAG GGCCTGCCGC CCCGACGTTG GCTGCGAGCC CTGGGCCTTC 
     6181 ACCCGAACTT GGGGGGTGGG GTGGGGAAAA GGAAGAAACG CGGGCGTATT GGCCCCAATG 
     6241 GGGTCTCGGT GGGGTATCGA CAGAGTGCCA GCCCTGGGAC CGAACCCCGC GTTTATGAAC 
     6301 AAACGACCCA ACACCGTGCG TTTTATTCTG TCTTTTTATT GCCGTCATAG CGCGGGTTCC 
     6361 TTCCGGTATT GTCTCCTTCC GTGTTTCAGT TAGCCTCCCC CTAGGGTGGG CGAAGAACTC 
     6421 CAGCATGAGA TCCCCGCGCT GGAGGATCAT CCAGCCGGCG TCCCGGAAAA CGATTCCGAA 
     6481 GCCCAACCTT TCATAGAAGG CGGCGGTGGA ATCGAAATCT CGTGATGGCA GGTTGGGCGT 
     6541 CGCTTGGTCG GTCATTTCGA ACCCCAGAGT CCCGCTCAGA AGAACTCGTC AAGAAGGCGA 
     6601 TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG GCGATACCGT AAAGCACGAG GAAGCGGTCA 
     6661 GCCCATTCGC CGCCAAGCTC TTCAGCAATA TCACGGGTAG CCAACGCTAT GTCCTGATAG 
     6721 CGATCCGCCA CACCCAGCCG GCCACAGTCG ATGAATCCAG AAAAGCGGCC ATTTTCCACC 
     6781 ATGATATTCG GCAAGCAGGC ATCGCCATGG GTCACGACGA GATCCTCGCC GTCGGGCATG 
     6841 CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT GGCGCGAGCC CCTGATGCTC TTCGTCCAGA 
     6901 TCATCCTGAT CGACAAGACC GGCTTCCATC CGAGTACGTG CTCGCTCGAT GCGATGTTTC 
     6961 GCTTGGTGGT CGAATGGGCA GGTAGCCGGA TCAAGCGTAT GCAGCCGCCG CATTGCATCA 
     7021 GCCATGATGG ATACTTTCTC GGCAGGAGCA AGGTGAGATG ACAGGAGATC CTGCCCCGGC 
     7081 ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC GCTTCAGTGA CAACGTCGAG CACAGCTGCG 
     7141 CAAGGAACGC CCGTCGTGGC CAGCCACGAT AGCCGCGCTG CCTCGTCTTG CAGTTCATTC 
     7201 AGGGCACCGG ACAGGTCGGT CTTGACAAAA AGAACCGGGC GCCCCTGCGC TGACAGCCGG 
     7261 AACACGGCGG CATCAGAGCA GCCGATTGTC TGTTGTGCCC AGTCATAGCC GAATAGCCTC 
     7321 TCCACCCAAG CGGCCGGAGA ACCTGCGTGC AATCCATCTT GTTCAATCAT GCGAAACGAT 
     7381 CCTCATCCTG TCTCTTGATC GATCTTTGCA AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA 
     7441 CTACTTCTGG AATAGCTCAG AGGCCGAGGC GGCCTCGGCC TCTGCATAAA TAAAAAAAAT 
     7501 TAGTCAGCCA TGGGGCGGAG AATGGGCGGA ACTGGGCGGA GTTAGGGGCG GGATGGGCGG 
     7561 AGTTAGGGGC GGGACTATGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     7621 TGCTGGGGAG CCTGGGGACT TTCCACACCT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     7681 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CCTAACTGAC ACACATTCCA 
     7741 CAGCTGGTTC TTTCCGCCTC AGGACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG 
     7801 GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG 
     7861 GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGCGC CCTGTAGCGG CGCATTAAGC 
     7921 GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC 
     7981 GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT 
     8041 CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA 
     8101 AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC 
     8161 CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA 
     8221 CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT 
     8281 TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTT
//