LOCUS       MR211708                8322 bp    DNA     circular  SYN 
DEFINITION  Asap1 (Myc-DDK-tagged) - Mouse ArfGAP with SH# domain, ankyrin repeat and PH domain1 (Asap1)
ACCESSION   NM_010026
VERSION     NM_010026.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4479..4570 
                     /label="Myc-DDK"  
     misc_feature    4600..5185 
                     /label="PolyA signal"  
     misc_feature    4657..4676 
                     /label="Restriction Enzyme"  
     misc_feature    5461..6133 
                     /label="ColE1"  
     misc_feature    6563..7357 
                     /label="Kan/Neo"  
     misc_feature    7392..7694 
                     /label="SV40 ori"  
     misc_feature    7780..8257 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4461..4467 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4461 
                     /label="Asap1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAGATCTTCA GCCTCCCGGC TCTCCAGTTT TTCCTCCAGA GATTCACTAT 
     1081 GGAATCGGAT GCCGGACCAG ATCTCCGTGT CCGAGTTCAT CGCCGAGACC ACCGAGGACT 
     1141 ACAACTCGCC CACCACGTCC AGCTTCACCA CGCGGCTGCA CAACTGCAGG AACACCGTCA 
     1201 CGCTGCTGGA GGAGGCTCTA GACCAAGATA GAACAGCCCT ACAGAAAGTG AAGAAATCTG 
     1261 TAAAAGCAAT ATATAATTCC GGTCAAGATC ATGTACAAAA TGAAGAAAAC TATGCACAAG 
     1321 TTCTGGATAA GTTTGGAAGT AATTTTTTAA GCAGAGACAA CCCTGACCTT GGCACCGCTT 
     1381 TTGTCAAGTT TTCTACACTC ACAAAGGAAC TGTCCACGCT GCTGAAAAAT CTGCTCCAGG 
     1441 GTCTGAGCCA CAATGTGATC TTCACCTTGG ATTCCTTGTT AAAAGGAGAC TTGAAGGGGG 
     1501 TCAAAGGAGA TCTCAAGAAG CCATTTGACA AAGCCTGGAA AGATTATGAG ACGAAGTTTA 
     1561 CAAAAATTGA GAAGGAGAAA AGAGAGCACG CAAAACAGCA CGGGATGATC CGAACAGAGA 
     1621 TAACAGGCGC TGAGATTGCA GAAGAAATGG AAAAGGAACG GCGGCTTTTT CAGCTCCAGA 
     1681 TGTGTGAATA TCTCATTAAA GTAAATGAAA TCAAGACCAA AAAGGGTGTG GACCTGCTGC 
     1741 AGAACCTTAT AAAGTATTAC CATGCACAGT GCAATTTCTT TCAGGATGGC TTGAAAACGG 
     1801 CTGATAAACT GAAACAGTAC ATAGAAAAGC TGGCTGCTGA CTTATATAAT ATAAAGCAAA 
     1861 CCCAAGATGA AGAAAAAAAA CAGCTAACTG CACTCCGAGA TCTAATAAAA TCCTCTCTGC 
     1921 AACTTGATCC GAAAGAAGTA GGTGGTTTAT ATGTTGCCAG CAGGGCTAAC AGTTCTAGAA 
     1981 GAGACTCTCA GAGCCGGCAA GGTGGGTACA GCATGCATCA GCTCCAGGGC AACAAGGAGT 
     2041 ACGGCAGTGA AAAGAAGGGC TTCCTGCTGA AGAAGAGTGA TGGGATCCGG AAAGTGTGGC 
     2101 AGAGACGGAA GTGTGCGGTC AAGAACGGGA TCCTGACCAT CTCCCACGCA ACTTCCAACA 
     2161 GGCAGCCTGC TAAGTTAAAC CTCCTCACCT GCCAAGTGAA ACCGAATGCT GAGGACAAGA 
     2221 AGTCTTTTGA CCTGATATCA CATAATCGAA CATATCACTT TCAAGCAGAA GATGAGCAGG 
     2281 ATTATATAGC GTGGATATCA GTACTGACGA ATAGCAAAGA AGAGGCCCTA ACCATGGCCT 
     2341 TCCGTGGTGA GCAGAGCACA GGGGAGAATA GCCTGGAGGA CCTTACCAAA GCCATCATCG 
     2401 AGGATGTACA GCGGCTCCCT GGGAATGACA TCTGCTGTGA CTGCGGCTCA TCAGAACCCA 
     2461 CGTGGCTTTC AACCAACTTG GGTATTTTGA CCTGTATAGA ATGTTCCGGA ATCCATAGGG 
     2521 AAATGGGGGT TCATATTTCT CGCATTCAGT CTTTGGAACT AGACAAATTA GGAACTTCCG 
     2581 AACTCTTGCT GGCCAAGAAT GTAGGAAACA ATAGTTTTAA TGATATTATG GAAGCAAATT 
     2641 TACCCAGCCC TTCACCAAAG CCCACCCCTT CAAGTGATAT GACCGTGCGT AAAGAGTACA 
     2701 TCACTGCAAA GTATGTCGAC CACCGCTTCT CCAGGAAGAC CTGTGCCTCT TCCTCAGCAA 
     2761 AGCTGAACGA ATTGCTTGAG GCCATCAAGT CCAGGGACTT ACTTGCATTA ATCCAAGTCT 
     2821 ATGCAGAGGG GGTGGAGTTA ATGGAGCCAC TGCTGGAACC TGGGCAGGAG CTCGGGGAGA 
     2881 CAGCCCTTCA TCTTGCCGTC AGGACCGCAG ACCAGACATC TCTCCATCTG GTTGATTTCC 
     2941 TTGTACAAAA CTGTGGGAAC CTGGATAAGC AAACGTCTGT GGGGAATACA GTTCTGCACT 
     3001 ATTGCAGCAT GTACGGCAAG CCGGAGTGTC TGAAGCTGCT GCTTAGGAGC AAGCCCACCG 
     3061 TGGACATCGT TAACCAGAAT GGAGAAACTG CCTTGGATAT AGCAAAGAGA CTCAAAGCTA 
     3121 CCCAGTGTGA AGATCTGCTT TCCCAGGCTA AATCTGGAAA GTTCAACCCT CATGTCCACG 
     3181 TAGAGTATGA GTGGAATCTT CGACAGGACG AGATGGATGA GAGCGATGAC GATCTGGATG 
     3241 ACAAGCCAAG CCCGATCAAA AAGGAACGCT CGCCCAGACC ACAGAGCTTC TGCCACTCCT 
     3301 CCAGCATCTC GCCACAGGAC AAGCTGGCAC TGCCAGGGTT CAGCACTCCA CGGGACAAGC 
     3361 AGCGGCTCTC CTACGGAGCC TTCACCAACC AGATCTTCGC CTCTACGAGC ACAGATTTGC 
     3421 CCACATCACC CACCAGTGAG GCTCCCCCTT TGCCACCTCG GAACGCCGGG AAAGGTCCAA 
     3481 CTGGCCCACC TTCAACACTC CCTCTAGGCA CCCAGACCTC TAGTGGCAGC TCCACCCTAT 
     3541 CCAAGAAGAG GCCTCCTCCC CCACCACCAG GACACAAGAG AACCCTGTCT GACCCTCCCA 
     3601 GCCCACTACC TCACGGGCCC CCAAACAAAG GCGCAATTCC TTGGGGTAAT GATGTGGGCC 
     3661 CATTATCTTC AAGTAAGACG GCCAACAAGT TTGAGGGGCT GTCTCAGCAA GCAAGCACCA 
     3721 GTTCTGCTAA GACTGCCCTT GGCCCGAGAG TGCTTCCTAA ACTACCTCAG AAAGTGGCAC 
     3781 TAAGGAAGAC GGAGACCAGC CATCATCTCT CCCTCGACAG AACCAACATC CCACCTGAGA 
     3841 CTTTTCAGAA ATCATCACAG TTGACAGAGT TACCCCAAAA GCCACCACTT GGAGAGCTGC 
     3901 CCCCGAAGCC TGTGGAACTG GCCCCCAAGC CCCAAGTTGG AGAGCTGCCA CCTAAGCCTG 
     3961 GAGAGCTACC CCCTAAGCCC CAATTAGGTG ACCTGCCCCC CAAGCCACAG CTCTCAGACT 
     4021 TACCTCCCAA GCCACAGATG AAGGACCTGC CCCCTAAGCC GCAGCTGGGG GATCTGCTGG 
     4081 CAAAGTCCCA GGCTGGCGAT GTCTCAGCCA AGGTGCAGCC ACCCTCAGAG GTCACACAGA 
     4141 GGTCACACAC CGGGGATCTG TCTCCAAATG TACAGTCCAG AGATGCCATC CAGAAGCAAG 
     4201 CATCTGAAGA CTCCAACGAC CTCACACCCA CGCTGCCAGA GACACCCGTA CCACTGCCGA 
     4261 GAAAAATCAA TACGGGGAAA AATAAAGTGA GGCGGGTGAA GACCATTTAT GACTGCCAGG 
     4321 CAGATAATGA CGACGAACTC ACATTTATTG AGGGGGAAGT GATCATTGTC ACCGGGGAAG 
     4381 AGGACCAGGA GTGGTGGATC GGGCATATCG AAGGACAGCC TGAAAGGAAG GGTGTCTTTC 
     4441 CAGTGTCCTT TGTCCACATC CTGTCTGACA CGCGTACGCG GCCGCTCGAG CAGAAACTCA 
     4501 TCTCAGAAGA GGATCTGGCA GCAAATGATA TCCTGGATTA CAAGGATGAC GACGATAAGG 
     4561 TTTAAACGGC CGGCCGCGGT CATAGCTGTT TCCTGAACAG ATCCCGGGTG GCATCCCTGT 
     4621 GACCCCTCCC CAGTGCCTCT CCTGGCCCTG GAAGTTGCCA CTCCAGTGCC CACCAGCCTT 
     4681 GTCCTAATAA AATTAAGTTG CATCATTTTG TCTGACTAGG TGTCCTTCTA TAATATTATG 
     4741 GGGTGGAGGG GGGTGGTATG GAGCAAGGGG CAAGTTGGGA AGACAACCTG TAGGGCCTGC 
     4801 GGGGTCTATT GGGAACCAAG CTGGAGTGCA GTGGCACAAT CTTGGCTCAC TGCAATCTCC 
     4861 GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA 
     4921 TGCATGACCA GGCTCAGCTA ATTTTTGTTT TTTTGGTAGA GACGGGGTTT CACCATATTG 
     4981 GCCAGGCTGG TCTCCAACTC CTAATCTCAG GTGATCTACC CACCTTGGCC TCCCAAATTG 
     5041 CTGGGATTAC AGGCGTGAAC CACTGCTCCC TTCCCTGTCC TTCTGATTTT AAAATAACTA 
     5101 TACCAGCAGG AGGACGTCCA GACACAGCAT AGGCTACCTG GCCATGCCCA ACCGGTGGGA 
     5161 CATTTGAGTT GCTTGCTTGG CACTGTCCTC TCATGCGTTG GGTCCACTCA GTAGATGCCT 
     5221 GTTGAATTGG GTACGCGGCC AGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG 
     5281 GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA 
     5341 GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA 
     5401 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG 
     5461 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT 
     5521 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG 
     5581 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC 
     5641 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT 
     5701 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA 
     5761 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC 
     5821 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC 
     5881 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT 
     5941 TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC 
     6001 TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT 
     6061 CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA 
     6121 ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC GACGTTGGCT GCGAGCCCTG GGCCTTCACC 
     6181 CGAACTTGGG GGGTGGGGTG GGGAAAAGGA AGAAACGCGG GCGTATTGGC CCCAATGGGG 
     6241 TCTCGGTGGG GTATCGACAG AGTGCCAGCC CTGGGACCGA ACCCCGCGTT TATGAACAAA 
     6301 CGACCCAACA CCGTGCGTTT TATTCTGTCT TTTTATTGCC GTCATAGCGC GGGTTCCTTC 
     6361 CGGTATTGTC TCCTTCCGTG TTTCAGTTAG CCTCCCCCTA GGGTGGGCGA AGAACTCCAG 
     6421 CATGAGATCC CCGCGCTGGA GGATCATCCA GCCGGCGTCC CGGAAAACGA TTCCGAAGCC 
     6481 CAACCTTTCA TAGAAGGCGG CGGTGGAATC GAAATCTCGT GATGGCAGGT TGGGCGTCGC 
     6541 TTGGTCGGTC ATTTCGAACC CCAGAGTCCC GCTCAGAAGA ACTCGTCAAG AAGGCGATAG 
     6601 AAGGCGATGC GCTGCGAATC GGGAGCGGCG ATACCGTAAA GCACGAGGAA GCGGTCAGCC 
     6661 CATTCGCCGC CAAGCTCTTC AGCAATATCA CGGGTAGCCA ACGCTATGTC CTGATAGCGA 
     6721 TCCGCCACAC CCAGCCGGCC ACAGTCGATG AATCCAGAAA AGCGGCCATT TTCCACCATG 
     6781 ATATTCGGCA AGCAGGCATC GCCATGGGTC ACGACGAGAT CCTCGCCGTC GGGCATGCTC 
     6841 GCCTTGAGCC TGGCGAACAG TTCGGCTGGC GCGAGCCCCT GATGCTCTTC GTCCAGATCA 
     6901 TCCTGATCGA CAAGACCGGC TTCCATCCGA GTACGTGCTC GCTCGATGCG ATGTTTCGCT 
     6961 TGGTGGTCGA ATGGGCAGGT AGCCGGATCA AGCGTATGCA GCCGCCGCAT TGCATCAGCC 
     7021 ATGATGGATA CTTTCTCGGC AGGAGCAAGG TGAGATGACA GGAGATCCTG CCCCGGCACT 
     7081 TCGCCCAATA GCAGCCAGTC CCTTCCCGCT TCAGTGACAA CGTCGAGCAC AGCTGCGCAA 
     7141 GGAACGCCCG TCGTGGCCAG CCACGATAGC CGCGCTGCCT CGTCTTGCAG TTCATTCAGG 
     7201 GCACCGGACA GGTCGGTCTT GACAAAAAGA ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC 
     7261 ACGGCGGCAT CAGAGCAGCC GATTGTCTGT TGTGCCCAGT CATAGCCGAA TAGCCTCTCC 
     7321 ACCCAAGCGG CCGGAGAACC TGCGTGCAAT CCATCTTGTT CAATCATGCG AAACGATCCT 
     7381 CATCCTGTCT CTTGATCGAT CTTTGCAAAA GCCTAGGCCT CCAAAAAAGC CTCCTCACTA 
     7441 CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC CTCGGCCTCT GCATAAATAA AAAAAATTAG 
     7501 TCAGCCATGG GGCGGAGAAT GGGCGGAACT GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT 
     7561 TAGGGGCGGG ACTATGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     7621 TGGGGAGCCT GGGGACTTTC CACACCTGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     7681 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCCT AACTGACACA CATTCCACAG 
     7741 CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT 
     7801 TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT 
     7861 CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGCGCCCT GTAGCGGCGC ATTAAGCGCG 
     7921 GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT 
     7981 CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA 
     8041 AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA 
     8101 CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT 
     8161 TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC 
     8221 AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG 
     8281 TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TT
//