LOCUS       MR211692                8289 bp    DNA     circular  SYN 
DEFINITION  Mybpc2 (Myc-DDK-tagged) - Mouse myosin binding protein C, fast-type (Mybpc2), transcript variant 2
ACCESSION   NM_146189
VERSION     NM_146189.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4446..4537 
                     /label="Myc-DDK"  
     misc_feature    4567..5152 
                     /label="PolyA signal"  
     misc_feature    4624..4643 
                     /label="Restriction Enzyme"  
     misc_feature    5428..6100 
                     /label="ColE1"  
     misc_feature    6530..7324 
                     /label="Kan/Neo"  
     misc_feature    7359..7661 
                     /label="SV40 ori"  
     misc_feature    7747..8224 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4428..4434 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4428 
                     /label="Mybpc2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCTGAGGCT AAACCAGCGG CCAAAAAGGC CTCCAAAGGC AAAGATGCCC 
     1081 CAAAGGAAGC CCCTGCAAAG CAGACTCCTG AAGAGCCCCC CAAAGAGGCC CCACCTGAGG 
     1141 ACCAATCCCC AACTGCGGAG GAGCCCACAG GCATCTTCCT GAAAAAGCCG GACTCTGTGT 
     1201 CAGTGGAGAC TGGGAAAGAC GCGGTGATTC TGGCCAAGGT GAACGGGAAG GAGCTCCCAG 
     1261 GCAAGCCGAC CATCAAGTGG TTCAAGGGGA AGTGGCAGGA GCTGGGCAGC AAGAGCGGAG 
     1321 CCCGGTTCAT CTTCAAGGAA TCTCATGACT CTACCAGCAA TGTGTACACC GTGGAGCTCC 
     1381 ACATTGGGAA GGTGGTCCTA GGGGACCGCG GGGATTACCG TCTAGAGATC AAAGCCAAGG 
     1441 ATGTCTGCGA CAGCTGCTCG TTCAACGTGG ATGTGGAGGC ACCCCGTCAG GACTCATCCG 
     1501 GTCAGAGCCT CGAGAGCTTC AAGCGTTCGG GTGACGGGAA GTCAGAAGAT GCAGGCGAGC 
     1561 TGGATTTCAG TGGCTTGCTG AAGAAGAGGG AAGTAGTTGA GGAGGAGAAG AAGAAGAAGA 
     1621 AAGATGATGA TGATCTGGGT ATACCTCCGG AGATTTGGGA GCTCCTGAAA GGGGCCAAGA 
     1681 AGAGCGAGTA CGAGAAGATC GCCTTCCAGT ACGGCATCAC TGACCTCCGT GGCATGCTGA 
     1741 AGCGGCTCAA GAAGGCCAAG GTGGAGGTCA AGAAGAGTGC AGCCTTCACT AAGAAGTTGG 
     1801 ACCCAGCCTA CCAAGTAGAC AGGGGCAACA AGATCAAGTT GGTGGTGGAG ATCAGTGACC 
     1861 CAGACCTTCC TCTCAAGTGG TTCAAGAATG GCCAGGAGAT CAAACCAAGC AGCAAGTATG 
     1921 TGTTTGAGAA CGTTGGGAAG AAACGAATTC TTACCATCAA CAAGTGCACG CTGGCGGATG 
     1981 ATGCTGCATA TGAGGTGGCA GTCCAAGATG AGAAGTGTTT CACTGAACTC TTTGTCAAAG 
     2041 AGCCCCCAGT CTTGATTGTC ACCCCACTGG AGGACCAGCA GGTATTCGTA GGTGACCGAG 
     2101 TGGAAATGTC GGTAGAGGTG TCAGAAGAGG GTGCACAGGT TATGTGGATG AAAGATGGTG 
     2161 TGGAGATGAC TCGGGAGGAT TCCTACAAGG CGCGCTACCG CTTCAAGAAG GACGGGAAAC 
     2221 GGCACATCCT CATCTACTCT GATGTGGCCC AGGAGGATGG GGGCCGCTAC CAGGTCATAA 
     2281 CCAATGGTGG CCAGTGTGAG GCTGAACTCA TTGTGGAAGA GAAGCAACTG GAGGTCCTGC 
     2341 AGGACATTGC TGACCTGACA GTGAAGGCTG CAGAGCAGGC TGTGTTCAAG TGTGAAGTGT 
     2401 CTGATGAGAA GGTGACAGGC AAATGGTACA AGAATGGCGT GGAGGTACGA CCCAGCAAGA 
     2461 GGATCACCAT CTCCCATGTG GGCAGATTCC ACAAGCTGGT GATTGATGAC GTCCGCCCTG 
     2521 AGGATGAGGG AGACTACACA TTCGTGCCCG ATGGCTATGC CCTATCCCTC TCAGCCAAGC 
     2581 TCAACTTTTT GGAAATCAAA GTGGAGTACG TACCTAAGCA AGAGCCCCCG AAGATCCACC 
     2641 TGGACTGCTC AGGGAAGACC TCAGATAACT CAATTGTTGT GGTGGCTGGA AACAAGCTGC 
     2701 GGCTGGATGT GGCCATCACA GGGGAACCAC CTCCCACTGC TACCTGGCTA AGGGGAGATG 
     2761 AGGTGTTCAC AGCCACAGAA GGCAGGACAC ACATCGAGCA GAGGCCAGAC TGCAGCAGCT 
     2821 TTGTGATCGA GAGTGCGGAG CGGTCGGATG AGGGCCGCTA CACCATCAAA GTCACCAATC 
     2881 CTGCGGGCGA GGACGTGGCC TCCATTTTCC TGCGGGTTGT GGATGTTCCT GATCCTCCAG 
     2941 AGGCTGTCCG TGTCACCTCA GTTGGAGAAG ACTGGGCCAT TCTGGTCTGG GAGCCACCCA 
     3001 AGTATGATGG AGGACAGCCA GTGACTGGGT ACTTGATGGA GCGGAAGAAG AAAGGCTCCC 
     3061 AGCGCTGGAT GAAGATCAAC TTTGAGGTCT TCACTGACAC CACCTACGAG TCCACAAAGA 
     3121 TGATCGAGGG CGTCCTTTAT GAGATGCGGG TCTTCGCTGT CAACGCCATT GGGGTCTCAC 
     3181 AGCCCAGCAT GAACACCAAG CCCTTCATGC CCATTGCTCC CACGAGTGCA CCACAACACC 
     3241 TCACCGTGGA GGATGTGACG GACACCACCA CCACACTTAA GTGGAGACCT CCAGATCGGA 
     3301 TTGGTGCTGG TGGCATTGAT GGCTACCTGG TGGAGTACTG CCTGGAGGGC TCTGAGGAAT 
     3361 GGGTACCGGC TAACAAGGAG CCAGTGGAAC GCTGTGGCTT CACTGTCAAG GATCTCCCAA 
     3421 CTGGAGCCAG GATCCTCTTC CGGGTTGTTG GGGTCAACAT CGCAGGGCGC AGTGAACCAG 
     3481 CCACCCTCCT TCAGCCAGTC ACTATCAGGG AGATTGTTGA GCAACCTAAG ATCCGCCTTC 
     3541 CCCGCCATCT TCGTCAGACT TATATCCGAA AAGTTGGGGA GGCCCTCAAC CTCGTCATCC 
     3601 CCTTCCAGGG CAAGCCCCGG CCTCAGGTGG TGTGGACCAA GGGTGGGGCT CCCTTGGATA 
     3661 CCTCCCGTGT GAATGTGCGG ACTAGTGACT TCGACACAGT GTTCTTCGTG CGACAGGCGG 
     3721 CCCGCTCTGA CTCCGGAGAG TATGAGCTGA GTGTGCAGAT TGAAAACATG AAGGACACTG 
     3781 CTACCATACG CATCCGGGTC GTGGAAAAGG CAGGGCCAGC AGAGAATGTG ATGGTGAAGG 
     3841 AGGTGTGGGG CACAAATGCT CTGGTGGAGT GGCAGCCGCC CAAGGACGAC GGGAACAGTG 
     3901 AGATCACAGG CTACTTTGTC CAGAAGGCTG ACAAAAAGAC CATGGAGTGG TTCAATGTCT 
     3961 ACGAACACAA TCGCCACACC AGCTGTACGG TGTCTGACCT CATTGTGGGT AATGAGTACT 
     4021 ACTTCCGTAT CTTCAGCGAG AACATCTGCG GTCTCAGTGA CTCGCCAGGT GTCTCCAAGA 
     4081 ACACAGCTCG AATTCTCAAG ACAGGAATCA CCTTAAAACC ACTGGAGTAC AAGGAGCATG 
     4141 ATTTCCGGAC GGCTCCCAAG TTCCTGACCC CGCTGATGGA CCGGGTCGTG GTGGCGGGAT 
     4201 ATACCGCAGC TCTCAACTGT GCCGTCAGAG GCCACCCTAA GCCAAAGGTG GTGTGGATGA 
     4261 AGAACAAGAT GGAAATCCAT GAAGATCCCA AATTCCTCAT AACCAACTAC CAGGGCATCC 
     4321 TGACACTGAA CATCCGCCGA CCGTCACCCT TTGATGCAGG GACCTATTCC TGCCGCGCCT 
     4381 TCAATGAGCT GGGGGAGGCC CTGGCTGAAT GCAAACTGGA TGTCCGAGTG CCACAGACGC 
     4441 GTACGCGGCC GCTCGAGCAG AAACTCATCT CAGAAGAGGA TCTGGCAGCA AATGATATCC 
     4501 TGGATTACAA GGATGACGAC GATAAGGTTT AAACGGCCGG CCGCGGTCAT AGCTGTTTCC 
     4561 TGAACAGATC CCGGGTGGCA TCCCTGTGAC CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA 
     4621 GTTGCCACTC CAGTGCCCAC CAGCCTTGTC CTAATAAAAT TAAGTTGCAT CATTTTGTCT 
     4681 GACTAGGTGT CCTTCTATAA TATTATGGGG TGGAGGGGGG TGGTATGGAG CAAGGGGCAA 
     4741 GTTGGGAAGA CAACCTGTAG GGCCTGCGGG GTCTATTGGG AACCAAGCTG GAGTGCAGTG 
     4801 GCACAATCTT GGCTCACTGC AATCTCCGCC TCCTGGGTTC AAGCGATTCT CCTGCCTCAG 
     4861 CCTCCCGAGT TGTTGGGATT CCAGGCATGC ATGACCAGGC TCAGCTAATT TTTGTTTTTT 
     4921 TGGTAGAGAC GGGGTTTCAC CATATTGGCC AGGCTGGTCT CCAACTCCTA ATCTCAGGTG 
     4981 ATCTACCCAC CTTGGCCTCC CAAATTGCTG GGATTACAGG CGTGAACCAC TGCTCCCTTC 
     5041 CCTGTCCTTC TGATTTTAAA ATAACTATAC CAGCAGGAGG ACGTCCAGAC ACAGCATAGG 
     5101 CTACCTGGCC ATGCCCAACC GGTGGGACAT TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA 
     5161 TGCGTTGGGT CCACTCAGTA GATGCCTGTT GAATTGGGTA CGCGGCCAGC GGCGAGCGGT 
     5221 ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA 
     5281 GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC 
     5341 GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG 
     5401 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT 
     5461 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG 
     5521 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG 
     5581 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG 
     5641 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC 
     5701 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG 
     5761 GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT 
     5821 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG 
     5881 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC 
     5941 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT 
     6001 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT 
     6061 TAAATCAATC TAAAGTATAT ATGAGTAACC TGAGGCTATG GCAGGGCCTG CCGCCCCGAC 
     6121 GTTGGCTGCG AGCCCTGGGC CTTCACCCGA ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA 
     6181 AACGCGGGCG TATTGGCCCC AATGGGGTCT CGGTGGGGTA TCGACAGAGT GCCAGCCCTG 
     6241 GGACCGAACC CCGCGTTTAT GAACAAACGA CCCAACACCG TGCGTTTTAT TCTGTCTTTT 
     6301 TATTGCCGTC ATAGCGCGGG TTCCTTCCGG TATTGTCTCC TTCCGTGTTT CAGTTAGCCT 
     6361 CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT GAGATCCCCG CGCTGGAGGA TCATCCAGCC 
     6421 GGCGTCCCGG AAAACGATTC CGAAGCCCAA CCTTTCATAG AAGGCGGCGG TGGAATCGAA 
     6481 ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG GTCGGTCATT TCGAACCCCA GAGTCCCGCT 
     6541 CAGAAGAACT CGTCAAGAAG GCGATAGAAG GCGATGCGCT GCGAATCGGG AGCGGCGATA 
     6601 CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT TCGCCGCCAA GCTCTTCAGC AATATCACGG 
     6661 GTAGCCAACG CTATGTCCTG ATAGCGATCC GCCACACCCA GCCGGCCACA GTCGATGAAT 
     6721 CCAGAAAAGC GGCCATTTTC CACCATGATA TTCGGCAAGC AGGCATCGCC ATGGGTCACG 
     6781 ACGAGATCCT CGCCGTCGGG CATGCTCGCC TTGAGCCTGG CGAACAGTTC GGCTGGCGCG 
     6841 AGCCCCTGAT GCTCTTCGTC CAGATCATCC TGATCGACAA GACCGGCTTC CATCCGAGTA 
     6901 CGTGCTCGCT CGATGCGATG TTTCGCTTGG TGGTCGAATG GGCAGGTAGC CGGATCAAGC 
     6961 GTATGCAGCC GCCGCATTGC ATCAGCCATG ATGGATACTT TCTCGGCAGG AGCAAGGTGA 
     7021 GATGACAGGA GATCCTGCCC CGGCACTTCG CCCAATAGCA GCCAGTCCCT TCCCGCTTCA 
     7081 GTGACAACGT CGAGCACAGC TGCGCAAGGA ACGCCCGTCG TGGCCAGCCA CGATAGCCGC 
     7141 GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA CCGGACAGGT CGGTCTTGAC AAAAAGAACC 
     7201 GGGCGCCCCT GCGCTGACAG CCGGAACACG GCGGCATCAG AGCAGCCGAT TGTCTGTTGT 
     7261 GCCCAGTCAT AGCCGAATAG CCTCTCCACC CAAGCGGCCG GAGAACCTGC GTGCAATCCA 
     7321 TCTTGTTCAA TCATGCGAAA CGATCCTCAT CCTGTCTCTT GATCGATCTT TGCAAAAGCC 
     7381 TAGGCCTCCA AAAAAGCCTC CTCACTACTT CTGGAATAGC TCAGAGGCCG AGGCGGCCTC 
     7441 GGCCTCTGCA TAAATAAAAA AAATTAGTCA GCCATGGGGC GGAGAATGGG CGGAACTGGG 
     7501 CGGAGTTAGG GGCGGGATGG GCGGAGTTAG GGGCGGGACT ATGGTTGCTG ACTAATTGAG 
     7561 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCTGGTTGC 
     7621 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     7681 ACACCCTAAC TGACACACAT TCCACAGCTG GTTCTTTCCG CCTCAGGACT CTTCCTTTTT 
     7741 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT 
     7801 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC 
     7861 GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT 
     7921 ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG 
     7981 TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT 
     8041 GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA 
     8101 TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA 
     8161 CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA 
     8221 GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC 
     8281 GCGAATTTT
//