LOCUS       MR211691                8286 bp    DNA     circular  SYN 
DEFINITION  Polr1b (Myc-DDK-tagged) - Mouse polymerase (RNA) I polypeptide B (Polr1b)
ACCESSION   NM_009086
VERSION     NM_009086.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4443..4534 
                     /label="Myc-DDK"  
     misc_feature    4564..5149 
                     /label="PolyA signal"  
     misc_feature    4621..4640 
                     /label="Restriction Enzyme"  
     misc_feature    5425..6097 
                     /label="ColE1"  
     misc_feature    6527..7321 
                     /label="Kan/Neo"  
     misc_feature    7356..7658 
                     /label="SV40 ori"  
     misc_feature    7744..8221 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4425..4431 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4425 
                     /label="Polr1b"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGATGTCGAC GGCCGGTGGC GGAACCTGCC CAGCGGGCCC AGCCTAAAGC 
     1081 ATTTAACCGA CCCCTCGTAC GGGATTCCCC CAGAGCAGCA AAAGGCAGCT TTGCAGGACC 
     1141 TGACGCGGGC GCACGTGGAC TCCTTCAACT ACGCGGCGCT GGAGGGGCTG AGCCACGCGG 
     1201 TGCAGGCCAT ACCTCCCTTT GAATTTGCTT TCAAAGATGA GCGCATATCT CTTACCATTG 
     1261 TGGATGCTGT CATCAGTCCA CCCTCAGTGC CCAAAGGGAC CATCTGCAAA GATCTCAACG 
     1321 TTTATCCAGC TGAGTGTCGT GGCCGAAAGA GCACCTACCG AGGGAGGCTG ACGGCTGATA 
     1381 TCAGCTGGGC CGTGAATGGA GTCCCCAAAG GGATCATTAA ACAGTTTCTC GGCTATGTTC 
     1441 CCATCATGGT GAAGTCCAAG CTTTGCAACT TATACAACCT TCCTCCTCGA GTCCTCATTG 
     1501 AGCACCATGA GGAGGCAGAG GAAATGGGCG GTTATTTTAT AATCAATGGC ATAGAAAAAG 
     1561 TCATCCGCAT GTTGATTGTG CCCCGGAGAA ATTTCCCCAT CGCCATGGTA AGGCCGAAAT 
     1621 GGAAAAGCAG AGGACTCGGC TACACTCAGT TTGGGGTCTC CATGCGCTGT GTGAGAGAAG 
     1681 AGCACTCCGC CGTGAACATG AACCTGCACT ATGTGGAGAA CGGCACGGTT ATGTTAAACT 
     1741 TTATTTACCG CAAAGAGCTC TTTTTCCTTC CTTTGGGATT TGCACTTAAG GCACTCGTGA 
     1801 GCTTTTCTGA TTATCAGATT TTCCAGGAGC TTATCAAGGG CAAAGAGGAG GACTCTTTCT 
     1861 TTAGGAATTC TGTTTCTCAG ATGCTGCGGA TTGTCATAGA AGAGGGCTGT CACTCACAGA 
     1921 AGCAGGTTCT TAACTATCTG GGAGAATGCT TCCGAGTGAA GCTCAGCCTT CCTGATTGGT 
     1981 ACCCTAACGT GGAAGCTGCC GAGTTCCTGC TTAACCAGTG CATCTGCATC CACCTGCAGT 
     2041 CCAACACTGA CAAGTTCTAC CTGCTCTGCC TCATGACCCG GAAGCTGTTC GCTTTAGCCA 
     2101 GAGGGGAGTG CATGGACGAC AACCCTGATA GCCTAGTGAA CCAAGAGGTC TTCAGCCCTG 
     2161 GGCAGCTCTT CCTGATGTTT CTGAAGGAAA AAATGGAAAA CTGGCTAGTG TCTATTAAAA 
     2221 TAGTTTTAGA TAAGAGGGCT CAGAAGGCCA ATGTTTCCAT AAACAACGAA AATTTGATGA 
     2281 AGATTTTTAG TATGGGAACA GAGCTAACAA GACCATTTGA ATATCTTCTT GCTACTGGGA 
     2341 ATCTGCGTTC TAAAACAGGT CTCGGCTTCC TGCAAGATTC TGGCCTGTGT GTTGTGGCTG 
     2401 ACAAGCTGAA CTTCCTTCGC TACCTCTCCC ATTTCCGCTG TGTGCACAGA GGGGCTGCCT 
     2461 TTGCCAAGAT GAGGACCACC ACCGTGCGCA GGCTGCTGCC AGAATCCTGG GGCTTCCTCT 
     2521 GCCCTGTGCA CACTCCAGAC GGGGCGCCTT GTGGGCTGTT GAACCACTTG ACTGCCGTGT 
     2581 GTGAGGTTGT TACCAAGTTT GTGTACACGG CATCTATTCC AGCCTTGCTC TGTGGCTTAG 
     2641 GAGTCACTCC TGTTGATACA GCGCCATGTC GACCGTACAG TGACTGCTAC CCTGTCCTGC 
     2701 TGGATGGCGT CATGGTGGGC TGGGTGGATA AGGACCTTGC TCCTGAAGTT GCAGACACTC 
     2761 TCCGTCGATT CAAGGTGTTG AGAGAAAAAA GAATTCCTCC CTGGATGGAG GTGGCCCTGA 
     2821 TTCCCATGAC AGGAAAACCA AGTCTGTACC CAGGTCTGTT CCTCTTTACC ACTCCCTGCA 
     2881 GGCTGGTGAG GCCTGTGCAG AACTTGGAAC TGGGCAGAGA AGAGCTCATT GGAACCATGG 
     2941 AACAGCTCTT CATGAACGTC GCCATCTTTG AGGACGAGGT GTTTGGTGGC ATCTCCACAC 
     3001 ACCAGGAGCT CTTCCCTCAC AGCCTGCTGA GCGTGATCGC CAACTTCATC CCCTTCTCGG 
     3061 ATCACAACCA GAGTCCTCGG AACATGTACC AGTGCCAGAT GGGTAAGCAG ACCATGGGCT 
     3121 TTCCGCTGCT CACTTACCAA AACCGATCAG ATAATAAACT CTATCGACTC CAAACCCCTC 
     3181 AGAGCCCTCT AGTGAGACCG TGCATGTATG ATTTCTATGA CATGGACAAC TATCCGATCG 
     3241 GGACAAACGC CATTGTAGCT GTGATCTCCT ACACTGGCTA TGATATGGAG GACGCCATGA 
     3301 TTGTCAACAA GGCTTCCTGG GAACGAGGCT TCGCTCATGG AAGCGTCTAC AAGTCTGAGT 
     3361 TCATAGACCT CTCAGAGAAG TTTAAGCAGG GAGAGGATAA CCTGGTGTTC GGGGTCAAGC 
     3421 CTGGTGACCC TCGGGTGATG CAGAAGCTGG ACGATGATGG GTTGCCATTC ATAGGCGCAA 
     3481 AGCTGGAGTA TGGCGACCCT TACTACAGCT ACCTCAACCT TAACACTGGG GAAGGCTTCG 
     3541 TGGTTTACTA CAAGAGTAAA GAAAACTGTG TTGTGGACAA CATCAAAGTG TGTAGTAACG 
     3601 ACATGGGCAG TGGGAAGTTC AAGTGTATCT GCATCACAGT CCGCATCCCC CGGAACCCAA 
     3661 CCATTGGAGA TAAGTTTGCC AGCCGACATG GTCAGAAGGG CATTTTGAGC AGATTGTGGC 
     3721 CAGCCGAGGA CATGCCTTTC ACTGAGAGTG GCATGATGCC GGACATTCTG TTTAATCCTC 
     3781 ACGGGTTTCC CTCCCGTATG ACCATAGGTA TGTTAATCGA GAGCATGGCT GGGAAGTCTG 
     3841 CAGCTTTGCA CGGTCTCTGC CATGATGCTA CACCCTTCAT CTTCTCCGAG GAGAACTCTG 
     3901 CCTTAGAGTA CTTTGGTGAG ATGTTAAAGG CTGCTGGCTA CAACTTCTAC GGCACCGAGA 
     3961 GACTGTATAG TGGCATCAGT GGGATGGAGC TGGAGGCTGA CATCTTCATT GGTGTGGTTT 
     4021 ATTACCAGCG CTTACGACAC ATGGTGTCAG ACAAATTTCA AGTCAGAACA ACTGGAGCCA 
     4081 GGGACAAAGT CACCAACCAG CCCCTCGGGG GACGAAATGT TCAGGGTGGA ATCCGCTTTG 
     4141 GGGAGATGGA GCGGGATGCT CTGTTGGCAC ACGGTACGTC TTTCCTTCTT CATGACCGGC 
     4201 TTTTCAACTG CTCTGACCGT TCTGTCGCTC ACATGTGTGT AGAGTGTGGC AGTTTGCTTT 
     4261 CTCCGCTACT TGAGAAGCCT CCTCCGTCTT GGTCTGCGAT GCGCAACAGA AAGTACAACT 
     4321 GCACTGTCTG TGGCCGCAGT GACACCATCG ACACCGTCTC TGTGCCGTAT GTCTTCCGGT 
     4381 ACTTTGTAGC TGAGCTGGCA GCCATGAACA TCAAAGTGAA ACTGGATGTC ATCACGCGTA 
     4441 CGCGGCCGCT CGAGCAGAAA CTCATCTCAG AAGAGGATCT GGCAGCAAAT GATATCCTGG 
     4501 ATTACAAGGA TGACGACGAT AAGGTTTAAA CGGCCGGCCG CGGTCATAGC TGTTTCCTGA 
     4561 ACAGATCCCG GGTGGCATCC CTGTGACCCC TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT 
     4621 GCCACTCCAG TGCCCACCAG CCTTGTCCTA ATAAAATTAA GTTGCATCAT TTTGTCTGAC 
     4681 TAGGTGTCCT TCTATAATAT TATGGGGTGG AGGGGGGTGG TATGGAGCAA GGGGCAAGTT 
     4741 GGGAAGACAA CCTGTAGGGC CTGCGGGGTC TATTGGGAAC CAAGCTGGAG TGCAGTGGCA 
     4801 CAATCTTGGC TCACTGCAAT CTCCGCCTCC TGGGTTCAAG CGATTCTCCT GCCTCAGCCT 
     4861 CCCGAGTTGT TGGGATTCCA GGCATGCATG ACCAGGCTCA GCTAATTTTT GTTTTTTTGG 
     4921 TAGAGACGGG GTTTCACCAT ATTGGCCAGG CTGGTCTCCA ACTCCTAATC TCAGGTGATC 
     4981 TACCCACCTT GGCCTCCCAA ATTGCTGGGA TTACAGGCGT GAACCACTGC TCCCTTCCCT 
     5041 GTCCTTCTGA TTTTAAAATA ACTATACCAG CAGGAGGACG TCCAGACACA GCATAGGCTA 
     5101 CCTGGCCATG CCCAACCGGT GGGACATTTG AGTTGCTTGC TTGGCACTGT CCTCTCATGC 
     5161 GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA TTGGGTACGC GGCCAGCGGC GAGCGGTATC 
     5221 AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA 
     5281 CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT 
     5341 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG 
     5401 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG 
     5461 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG 
     5521 CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC 
     5581 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA 
     5641 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG 
     5701 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC 
     5761 TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC 
     5821 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG 
     5881 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT 
     5941 GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT 
     6001 CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA 
     6061 ATCAATCTAA AGTATATATG AGTAACCTGA GGCTATGGCA GGGCCTGCCG CCCCGACGTT 
     6121 GGCTGCGAGC CCTGGGCCTT CACCCGAACT TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC 
     6181 GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG TGGGGTATCG ACAGAGTGCC AGCCCTGGGA 
     6241 CCGAACCCCG CGTTTATGAA CAAACGACCC AACACCGTGC GTTTTATTCT GTCTTTTTAT 
     6301 TGCCGTCATA GCGCGGGTTC CTTCCGGTAT TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC 
     6361 CCTAGGGTGG GCGAAGAACT CCAGCATGAG ATCCCCGCGC TGGAGGATCA TCCAGCCGGC 
     6421 GTCCCGGAAA ACGATTCCGA AGCCCAACCT TTCATAGAAG GCGGCGGTGG AATCGAAATC 
     6481 TCGTGATGGC AGGTTGGGCG TCGCTTGGTC GGTCATTTCG AACCCCAGAG TCCCGCTCAG 
     6541 AAGAACTCGT CAAGAAGGCG ATAGAAGGCG ATGCGCTGCG AATCGGGAGC GGCGATACCG 
     6601 TAAAGCACGA GGAAGCGGTC AGCCCATTCG CCGCCAAGCT CTTCAGCAAT ATCACGGGTA 
     6661 GCCAACGCTA TGTCCTGATA GCGATCCGCC ACACCCAGCC GGCCACAGTC GATGAATCCA 
     6721 GAAAAGCGGC CATTTTCCAC CATGATATTC GGCAAGCAGG CATCGCCATG GGTCACGACG 
     6781 AGATCCTCGC CGTCGGGCAT GCTCGCCTTG AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC 
     6841 CCCTGATGCT CTTCGTCCAG ATCATCCTGA TCGACAAGAC CGGCTTCCAT CCGAGTACGT 
     6901 GCTCGCTCGA TGCGATGTTT CGCTTGGTGG TCGAATGGGC AGGTAGCCGG ATCAAGCGTA 
     6961 TGCAGCCGCC GCATTGCATC AGCCATGATG GATACTTTCT CGGCAGGAGC AAGGTGAGAT 
     7021 GACAGGAGAT CCTGCCCCGG CACTTCGCCC AATAGCAGCC AGTCCCTTCC CGCTTCAGTG 
     7081 ACAACGTCGA GCACAGCTGC GCAAGGAACG CCCGTCGTGG CCAGCCACGA TAGCCGCGCT 
     7141 GCCTCGTCTT GCAGTTCATT CAGGGCACCG GACAGGTCGG TCTTGACAAA AAGAACCGGG 
     7201 CGCCCCTGCG CTGACAGCCG GAACACGGCG GCATCAGAGC AGCCGATTGT CTGTTGTGCC 
     7261 CAGTCATAGC CGAATAGCCT CTCCACCCAA GCGGCCGGAG AACCTGCGTG CAATCCATCT 
     7321 TGTTCAATCA TGCGAAACGA TCCTCATCCT GTCTCTTGAT CGATCTTTGC AAAAGCCTAG 
     7381 GCCTCCAAAA AAGCCTCCTC ACTACTTCTG GAATAGCTCA GAGGCCGAGG CGGCCTCGGC 
     7441 CTCTGCATAA ATAAAAAAAA TTAGTCAGCC ATGGGGCGGA GAATGGGCGG AACTGGGCGG 
     7501 AGTTAGGGGC GGGATGGGCG GAGTTAGGGG CGGGACTATG GTTGCTGACT AATTGAGATG 
     7561 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC TGGTTGCTGA 
     7621 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     7681 CCCTAACTGA CACACATTCC ACAGCTGGTT CTTTCCGCCT CAGGACTCTT CCTTTTTCAA 
     7741 TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT 
     7801 TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGCG 
     7861 CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA 
     7921 CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC 
     7981 GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT 
     8041 TTACGGCACC TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG 
     8101 CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC 
     8161 TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG 
     8221 ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG 
     8281 AATTTT
//