LOCUS       MR211682                8280 bp    DNA     circular  SYN 
DEFINITION  Polr3b (Myc-DDK-tagged) - Mouse polymerase (RNA) III (DNA directed) polypeptide B (Polr3b)
ACCESSION   NM_027423
VERSION     NM_027423.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4437..4528 
                     /label="Myc-DDK"  
     misc_feature    4558..5143 
                     /label="PolyA signal"  
     misc_feature    4615..4634 
                     /label="Restriction Enzyme"  
     misc_feature    5419..6091 
                     /label="ColE1"  
     misc_feature    6521..7315 
                     /label="Kan/Neo"  
     misc_feature    7350..7652 
                     /label="SV40 ori"  
     misc_feature    7738..8215 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4419..4425 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4419 
                     /label="Polr3b"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGATGTTCTA GCCGAGGAGT TCGGGAGCCT GACTCCGGAG CAGCTGACTG 
     1081 CGCCGATCCC GACTGTGGAG GAAAAATGGA GGCTGCTTCC AGCATTTTTA AAGGTTAAAG 
     1141 GCCTGGTGAA ACAGCACATA GACTCCTTTA ACTATTTCAT TAATGTAGAG ATAAAGAAGA 
     1201 TAATGAAAGC CAATGAAAAG GTTACAAGTG ATGCTGACCC CATGTGGTAC TTAAAATACC 
     1261 TTAATATCTA TGTCGGACTT CCTGATGTTG AAGAAAGCTT CAATGTAACT AGACCAGTGT 
     1321 CCCCTCATGA GTGCCGTCTG AGGGACATGA CGTACTCCGC CCCAATCACA GTGGACATTG 
     1381 AGTATACCCG AGGCAGCCAG AGGATAATCC GCAACGCCTT ACCCATTGGC AGAATGCCCA 
     1441 TCATGCTCCG AAGCTCGAAC TGTGTTCTAA CAGGGAAAAC GCCGGCAGAG TTTGCCAAAC 
     1501 TGAATGAGTG TCCACTAGAC CCAGGTGGCT ACTTCATTGT TAAAGGAGTG GAAAAAGTCA 
     1561 TTCTTATCCA AGAGCAGCTG TCCAAGAACA GGATCATTGT GGAGGCCGAC AGGAAAGGAG 
     1621 CCGTTGGAGC CTCAGTTACC AGCTCCACCC ATGAGAAGAA AAGCAGAACC AACATGGCCG 
     1681 TGAAACAGGG TCGATTCTAT CTGAGGCACA ACACCCTGTC TGAAGATATC CCCATTGTCA 
     1741 TCATATTTAA GGCCATGGGT GTGGAGAGTG ACCAGGAAAT CGTGCAGATG ATTGGAACAG 
     1801 AGGAGCACGT GATGGCTGCA TTTGGGCCCA GCTTGGAGGA GTGCCAGAAA GCTCAGATCT 
     1861 TCACCCAGAT GCAGGCATTG AAGTATATAG GGAATAAAGT AAGAAGACAA AGAATGTGGG 
     1921 GAGGTGGACC AAAGAAAACC AAAATAGAAG AAGCAAGAGA ACTCCTGGCT TCTACCATTC 
     1981 TGACCCACGT ACCAGTGAAG GAATTCAATT TCCGAGCTAA GTGTATCTAC ACTGCAGTGA 
     2041 TGGTGCGAAG GGTGATCCTG GCCCAAGGAG ATAACAAGGT CGATGACAGA GACTATTATG 
     2101 GCAACAAACG ACTGGAATTG GCAGGACAGC TCTTATCTCT TCTTTTTGAA GATTTGTTTA 
     2161 AAAAGTTTAA TTCAGAAATG AAGAAGATTG CAGACCAAGT GATTCCTAAA CAGAGAGCAG 
     2221 CCCAGTTTGA CGTCGTTAAA CACATGCGCC AAGACCAGAT TACCAACGGC ATGGTCAATG 
     2281 CCATCTCCAC GGGAAATTGG TCTCTGAAGA GATTTAAGAT GGACCGACAG GGTGTGACCC 
     2341 AGGTGCTGTC TCGCTTGTCA TATATATCTG CACTCGGCAT GATGACAAGA ATCTCTTCCC 
     2401 AGTTTGAAAA GACGAGGAAA GTGAGTGGCC CTCGTTCTCT GCAGCCGTCT CAGTGGGGAA 
     2461 TGCTCTGTCC CTCCGACACA CCTGAAGGGG AGGCCTGTGG TTTGGTTAAG AACTTGGCCC 
     2521 TCATGACACA TATAACAACT GACATGGAAG ATGGACCCAT TATTAAGTTA GCTGGGAACT 
     2581 TGGGAGTAGA AGATGTGAAT TTATTATGTG GAGAAGAGCT CTCTTACCCA AATGTGTTTC 
     2641 TTGTCTTCCT GAATGGTAAC ATCCTTGGTG TCATTCGAGA CCATAAAAAG CTTGTCAGCA 
     2701 CATTCCGGCT GATGCGAAGA GCAGGCTATA TCAATGAATT TGTTTCTATC TCCACAAACC 
     2761 TTACGGATCG CTGTGTTTAT ATTTCCTCTG ATGGCGGGCG CTTGTGCAGG CCTTACATAA 
     2821 TTGTTAAGAA ACAAAAGCCA GCTGTCACAA ATAAGCACAT GGAAGAGTTG GCTCAAGGCT 
     2881 ACAGGAATTT TGAAGATTTC TTACATGAAA GTCTAGTTGA ATACTTAGAT GTGAATGAAG 
     2941 AAAATGACTG TAACATTGCG CTCTACGAAC ATACAATTAA TAAAGACACC ACCCACTTGG 
     3001 AGATTGAACC CTTCACTCTG CTTGGTGTTT GTGCCGGCCT GATCCCATAC CCTCATCACA 
     3061 ACCAGTCTCC AAGAAACACC TACCAGTGTG CTATGGGCAA GCAAGCCATG GGTACCATTG 
     3121 GCTACAATCA ACGGAACAGA ATTGATACAC TTATGTATCT ACTAGCATAT CCACAAAAGC 
     3181 CCATGGTTAA AACAAAAACA ATCGAGTTGA TAGATTTTGA GAAGTTGCCG GCTGGCCAGA 
     3241 ACGCAACTGT TGCCGTGATG AGCTACAGCG GCTATGACAT TGAGGACGCG CTGGTTTTGA 
     3301 ACAAGGCCTC TCTGGACAGA GGTTTTGGGC GTTGTCTTGT GTATAAAAAT GCTAAGTGCA 
     3361 CCTTGAAACG ATACACCAAT CAGACCTTTG ATAAGGTGAT GGGTCCCATG TTGGATGCTG 
     3421 CTACCAGAAA ACCCATCTGG CGACATGAAA TTTTAGATGC AGATGGCATT TGTTCTCCAG 
     3481 GTGAGAAAGT GGAAAACAAA CAGGTGCTTG TAAACAAGTC CATGCCCACA GTGACACAGA 
     3541 TTCCTTTGGA AGGGAGCAAC GTGCCACAGC AACCACAGTA TAAAGATGTG CCTATCACGT 
     3601 ACAAAGGTGC GACAGACTCC TACATCGAGA AAGTGATGAT CTCTTCCAAC GCCGAGGATG 
     3661 CTTTCCTCAT CAAGATGCTC CTGAGACAGA CGCGGCGCCC AGAGATTGGA GACAAATTCA 
     3721 GCAGTCGCCA TGGACAAAAG GGTGTCTGTG GCCTGATCGT CCCCCAGGAA GACATGCCGT 
     3781 TCTGTGATTC TGGCATCTGT CCCGACATCA TAATGAACCC ACACGGCTTC CCATCAAGAA 
     3841 TGACGGTTGG AAAGTTAATT GAGCTGCTGG CTGGCAAGGC AGGCGTGTTA GACGGCAGGT 
     3901 TCCACTACGG CACTGCCTTT GGAGGGAGTA AAGTGAAGGA TGTGTGTGAA GATCTCGTTC 
     3961 GCCATGGTTA CAACTACCTG GGGAAGGACT ACGTCACCTC AGGCATCACA GGTGAGCCCT 
     4021 TAGAAGCATA CATCTATTTT GGACCTGTGT ATTATCAGAA ACTGAAACAC ATGGTGCTGG 
     4081 ATAAAATGCA CGCCCGGGCC CGAGGACCAC GAGCTGTCCT TACCAGGCAG CCCACTGAAG 
     4141 GGCGGTCGCG CGATGGTGGC TTGCGACTTG GAGAAATGGA ACGCGACTGT CTAATCGGCT 
     4201 ATGGAGCGAG TATGCTTTTG CTGGAGAGAC TAATGATCTC AAGTGATGCC TTTGAGGTTG 
     4261 ACGTCTGCGG GCAGTGTGGA CTCCTGGGAT ATTCTGGCTG GTGCCACTAC TGCAAATCTT 
     4321 CGTGCCACGT GTCCTCCCTC CGCATCCCGT ATGCCTGCAA GCTGCTCTTC CAGGAGTTAC 
     4381 AGTCCATGAA CATCATCCCC AGGCTGAAAC TGGCCAAGTA CAACGAGACG CGTACGCGGC 
     4441 CGCTCGAGCA GAAACTCATC TCAGAAGAGG ATCTGGCAGC AAATGATATC CTGGATTACA 
     4501 AGGATGACGA CGATAAGGTT TAAACGGCCG GCCGCGGTCA TAGCTGTTTC CTGAACAGAT 
     4561 CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA GTGCCTCTCC TGGCCCTGGA AGTTGCCACT 
     4621 CCAGTGCCCA CCAGCCTTGT CCTAATAAAA TTAAGTTGCA TCATTTTGTC TGACTAGGTG 
     4681 TCCTTCTATA ATATTATGGG GTGGAGGGGG GTGGTATGGA GCAAGGGGCA AGTTGGGAAG 
     4741 ACAACCTGTA GGGCCTGCGG GGTCTATTGG GAACCAAGCT GGAGTGCAGT GGCACAATCT 
     4801 TGGCTCACTG CAATCTCCGC CTCCTGGGTT CAAGCGATTC TCCTGCCTCA GCCTCCCGAG 
     4861 TTGTTGGGAT TCCAGGCATG CATGACCAGG CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA 
     4921 CGGGGTTTCA CCATATTGGC CAGGCTGGTC TCCAACTCCT AATCTCAGGT GATCTACCCA 
     4981 CCTTGGCCTC CCAAATTGCT GGGATTACAG GCGTGAACCA CTGCTCCCTT CCCTGTCCTT 
     5041 CTGATTTTAA AATAACTATA CCAGCAGGAG GACGTCCAGA CACAGCATAG GCTACCTGGC 
     5101 CATGCCCAAC CGGTGGGACA TTTGAGTTGC TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG 
     5161 TCCACTCAGT AGATGCCTGT TGAATTGGGT ACGCGGCCAG CGGCGAGCGG TATCAGCTCA 
     5221 CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG 
     5281 AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA 
     5341 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA 
     5401 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC 
     5461 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC 
     5521 GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT 
     5581 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG 
     5641 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG 
     5701 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA 
     5761 CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG 
     5821 AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT 
     5881 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT 
     5941 TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG 
     6001 ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT 
     6061 CTAAAGTATA TATGAGTAAC CTGAGGCTAT GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC 
     6121 GAGCCCTGGG CCTTCACCCG AACTTGGGGG GTGGGGTGGG GAAAAGGAAG AAACGCGGGC 
     6181 GTATTGGCCC CAATGGGGTC TCGGTGGGGT ATCGACAGAG TGCCAGCCCT GGGACCGAAC 
     6241 CCCGCGTTTA TGAACAAACG ACCCAACACC GTGCGTTTTA TTCTGTCTTT TTATTGCCGT 
     6301 CATAGCGCGG GTTCCTTCCG GTATTGTCTC CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG 
     6361 GTGGGCGAAG AACTCCAGCA TGAGATCCCC GCGCTGGAGG ATCATCCAGC CGGCGTCCCG 
     6421 GAAAACGATT CCGAAGCCCA ACCTTTCATA GAAGGCGGCG GTGGAATCGA AATCTCGTGA 
     6481 TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT TTCGAACCCC AGAGTCCCGC TCAGAAGAAC 
     6541 TCGTCAAGAA GGCGATAGAA GGCGATGCGC TGCGAATCGG GAGCGGCGAT ACCGTAAAGC 
     6601 ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA AGCTCTTCAG CAATATCACG GGTAGCCAAC 
     6661 GCTATGTCCT GATAGCGATC CGCCACACCC AGCCGGCCAC AGTCGATGAA TCCAGAAAAG 
     6721 CGGCCATTTT CCACCATGAT ATTCGGCAAG CAGGCATCGC CATGGGTCAC GACGAGATCC 
     6781 TCGCCGTCGG GCATGCTCGC CTTGAGCCTG GCGAACAGTT CGGCTGGCGC GAGCCCCTGA 
     6841 TGCTCTTCGT CCAGATCATC CTGATCGACA AGACCGGCTT CCATCCGAGT ACGTGCTCGC 
     6901 TCGATGCGAT GTTTCGCTTG GTGGTCGAAT GGGCAGGTAG CCGGATCAAG CGTATGCAGC 
     6961 CGCCGCATTG CATCAGCCAT GATGGATACT TTCTCGGCAG GAGCAAGGTG AGATGACAGG 
     7021 AGATCCTGCC CCGGCACTTC GCCCAATAGC AGCCAGTCCC TTCCCGCTTC AGTGACAACG 
     7081 TCGAGCACAG CTGCGCAAGG AACGCCCGTC GTGGCCAGCC ACGATAGCCG CGCTGCCTCG 
     7141 TCTTGCAGTT CATTCAGGGC ACCGGACAGG TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC 
     7201 TGCGCTGACA GCCGGAACAC GGCGGCATCA GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA 
     7261 TAGCCGAATA GCCTCTCCAC CCAAGCGGCC GGAGAACCTG CGTGCAATCC ATCTTGTTCA 
     7321 ATCATGCGAA ACGATCCTCA TCCTGTCTCT TGATCGATCT TTGCAAAAGC CTAGGCCTCC 
     7381 AAAAAAGCCT CCTCACTACT TCTGGAATAG CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC 
     7441 ATAAATAAAA AAAATTAGTC AGCCATGGGG CGGAGAATGG GCGGAACTGG GCGGAGTTAG 
     7501 GGGCGGGATG GGCGGAGTTA GGGGCGGGAC TATGGTTGCT GACTAATTGA GATGCATGCT 
     7561 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCTGGTTG CTGACTAATT 
     7621 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCCTAA 
     7681 CTGACACACA TTCCACAGCT GGTTCTTTCC GCCTCAGGAC TCTTCCTTTT TCAATATTAT 
     7741 TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA 
     7801 AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA CGCGCCCTGT 
     7861 AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC 
     7921 AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC 
     7981 TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG 
     8041 CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA 
     8101 TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC 
     8161 CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT TTGATTTATA AGGGATTTTG 
     8221 CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//