LOCUS       MR211675                8265 bp    DNA     circular  SYN 
DEFINITION  Ascc3 (Myc-DDK-tagged) - Mouse activating signal cointegrator 1 complex subunit 3 (cDNA clone MGC:69928 IMAGE:6826790)
ACCESSION   BC059917
VERSION     BC059917.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4422..4513 
                     /label="Myc-DDK"  
     misc_feature    4543..5128 
                     /label="PolyA signal"  
     misc_feature    4600..4619 
                     /label="Restriction Enzyme"  
     misc_feature    5404..6076 
                     /label="ColE1"  
     misc_feature    6506..7300 
                     /label="Kan/Neo"  
     misc_feature    7335..7637 
                     /label="SV40 ori"  
     misc_feature    7723..8200 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4404..4410 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4404 
                     /label="Ascc3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGACAGTTTC TCCCTCATAT CAGATTCTGC ATATGTCGCC CAGAATGCAG 
     1081 CTAGAATTGT CCGTGCTCTC TTTGAAATTG CACTGAGGAA ACGTTGGCCT ACCATGACCT 
     1141 ACAGGCTTTT GAATCTTAGT AAAGTCATTG ACAAGAGACT CTGGGGTTGG GCTAGCCCTT 
     1201 TGAGACAATT TTCTGTGTTA CCACCACACA TTCTAACAAG ATTAGAAGAA AAAAACCTTA 
     1261 CTGTGGATAA ACTCAAAGAC ATGAGAAAAG ATGAAATAGG TCACATTTTG CATCATGTGA 
     1321 ATATTGGACT GAAGGTCAAA CAGTGTGTTC ATCAAATTCC TTCTGTTACA ATGGAAGCTT 
     1381 CCATTCAGCC CATCACACGG ACTGTCCTCC GAGTATCACT GAACATCCAC CCTGATTTCT 
     1441 CTTGGAATGA CCAGGTTCAT GGAACAGTGG GGGAACCATG GTGGATTTGG GTAGAAGATC 
     1501 CTACAAATGA CCATATTTAT CACTCGGAGT ACTTTCTAGC TCTTAAAAAA CAGGTTATTA 
     1561 ATAAGGAAGC TCAACTTCTG GTATTTACAA TTCCTATTTT TGAGCCTTTG CCCTCTCAGT 
     1621 ACTACATTCG AGCAGTGTCT GATCGATGGC TAGGAGCTGA AGCTGTTTGC ATTATCAACT 
     1681 TTCAACATCT GATTCTACCA GAGAGACATC CTCCCCATAC AGAATTATTG GACCTTCAAC 
     1741 CTTTACCAAT TACAGCTTTG GGGTGCAAAG CATATGAAGC TCTATATAAC TTCAGCCACT 
     1801 TTAACCCTGT ACAGACGCAG ATATTCCACA CACTGTATCA CACAGACTGT AATGTTCTGC 
     1861 TTGGCGCCCC CACCGGATCA GGAAAGACAG TTGCAGCTGA ACTAGCCATT TTCAGAGTTT 
     1921 TCAACAAATA TCCTACTTCA AAGGCAGTAT ATATTGCACC CCTCAAAGCT CTAGTACGTG 
     1981 AAAGAATGGA TGATTGGAAA ATTAGGATAG AAGAAAAACT TGGTAAAAAA GTTATTGAAC 
     2041 TAACTGGGGA TGTGACTCCA GACATGAAAT CCATTGCTAA GGCTGACCTT ATTGTCACCA 
     2101 CACCAGAAAA GTGGGATGGA GTTAGTCGAA GCTGGCAGAA TAGAAGCTAT GTTCAGCAGG 
     2161 TCAACATTCT CATCATAGAT GAGATCCATC TGCTAGGGGA GGAGAGAGGC CCAGTTTTAG 
     2221 AAGTCATTGT ATCTCGAACA AACTTTATCT CATCACACAC AGAAAAACCA GTTAGAATAG 
     2281 TTGGACTGTC CACTGCATTA GCTAATGCTA GAGATCTTGC TGACTGGCTC AACATTAAGC 
     2341 AGATGGGTTT GTTTAACTTC CGACCATCAG TTCGCCCAGT TCCACTGGAA GTTCACATTC 
     2401 AGGGTTTCCC AGGACAACAT TACTGCCCAC GCATGGCTAG TATGAACAAG CCTGCATTTC 
     2461 AGGCAATTAG AAGTCACTCT CCAGCCAAGC CTGTTTTGAT ATTTGTCTCA TCAAGACGTC 
     2521 AAACACGACT TACAGCCTTA GAGTTGATAG CATTTCTGGC CACTGAAGAA GACCCAAAGC 
     2581 AGTGGTTGAA TATGGATGAG CAAGAGATGG ACAACATCAT TGGAACAGTA CGAGATTCCA 
     2641 ACCTCAAGCT GACTCTAGCT TTTGGAATAG GAATGCATCA TGCTGGCCTG CATGAAAGAG 
     2701 ACCGAAAAAC TGTAGAAGAG CTGTTTGTGA ACTGTAAAGT TCAGGTTCTC ATTGCTACAA 
     2761 GCACATTAGC ATGGGGTGTA AACTTTCCAG CACACTTAGT AATTATTAAG GGAACTGAAT 
     2821 ATTATGATGG AAAGACAAGA CGTTATGTGG ATTTTCCCAT CACAGATGTG CTGCAGATGA 
     2881 TGGGACGAGC TGGGAGACCT CAGTTTGATG ACCAGGGCAA AGCTGTAATT CTAGTTCATG 
     2941 ATATAAAAAA AGATTTTTAT AAAAAGTTTC TTTATGAACC TTTCCCAGTA GAATCAAGCT 
     3001 TATTAGGAGT GTTGTCTGAC CACTTAAATG CAGAGATTGC TGGAGGTACA ATAACATCAA 
     3061 AGCAAGATGC AATGGATTAT ATCACCTGGA CTTACTTTTT CCGGCGTCTT ATCATGAATC 
     3121 CCAGCTACTA CAGCTTGGGT GACGTCAGCC AGGACTCCAT AAACAAGTTC CTGTCACATC 
     3181 TGATTGGGCA GTCTCTGGTT GAATTGGAGC TTTCCCACTG CATTGAAGTT GGAGAGGATA 
     3241 ATCGCACCAT TGAACCACTG ACATGTGGTC TAATTGCCTC CTATTACTAT TTGAAGCACA 
     3301 AAACTGTCAA AATGTTCAAG GACCGTTTGA AACCTGAGTG CAGCACTGAG GAACTGCTGT 
     3361 CAATTCTGAG TGATGCAGAA GAATACACAG ACTTACCAGT AAGACACAAT GAGGACCATA 
     3421 CGAATAATGA ATTGGCCAAA TGTCTTCCCA TTGAATTAAA TCCTCATTCA TTTGACAGCC 
     3481 CTCACACCAA AGCCCATCTC TTGCTTCAAG CGCATCTCAG CCGAGCCATG CTGCCCTGCC 
     3541 CAGATTATGA TACTGATACC AAAACAGTCT TAGACCAAGC TCTCAGAGTA TGCCAGGCAA 
     3601 TGCTGGATGT GGCTGCAAGC CAGGGATGGC TAGTGACCGT CCTGAATATC ACCCACCTGA 
     3661 TTCAGATGGT GATCCAAGGC CGATGGCTCA AGGACTCTTC TCTTCTCACA ATACCAAACA 
     3721 TAGAGCAGCA CCACCTTCAC CTTTTCAGGA AATGGAAGCC ACCTGTAAAG AGCTCACATG 
     3781 CTAAGTGCCG AACATCCATT GAGTGCCTTC CTGAGCTGAT CCATGCCTGT GAAGGGAAGG 
     3841 ACCATGTGTT CAGCTCCATG GTAGAGAAAG AGTTACAGCC TGCAAAAACA AAGCAGGCAT 
     3901 GGAATTTCTT ATCTCGCTTG CCAGTGATAA ATGTTGGCAT AAGTGTTAAA GGCTCATGGG 
     3961 ATGACTTAGT TGAAGGACAT AATGAACTCT CCATCTCAAC TCTGACAGCA GACAAACGGG 
     4021 ATGAAAACAA ATGGATCAAG TTGCATGCTG ATCAGGAGTA TGTGCTTCAA GTCAGCCTAC 
     4081 AGAGAGTCCA TTTTGGGCTT CCAAAGGGAA AACATGAGAA TCACGCAGTT ACTCCTCGAT 
     4141 TTCCAAAATT AAAAGATGAA GGCTGGTTTT TGATATTAGG AGAAGTGGAT AAGAGAGAGC 
     4201 TGATGGCTGT AAAACGAGTA GGATTTGTTC GAACACATCA TGATGCTTCC ATCTCGTTCT 
     4261 TTACTCCTGA AACACCTGGA AGATATATCT TTACCCTGTA TCTCATGAGT GACTGTTACC 
     4321 TCGGCCTAGA CCAGCAGTAT GACATTTATC TCAATGTCAT AAAAGCAAAC ATTTCTACAA 
     4381 AGGACTCTGA TGTCTTCACT GACTTGTCAG TAACGCGTAC GCGGCCGCTC GAGCAGAAAC 
     4441 TCATCTCAGA AGAGGATCTG GCAGCAAATG ATATCCTGGA TTACAAGGAT GACGACGATA 
     4501 AGGTTTAAAC GGCCGGCCGC GGTCATAGCT GTTTCCTGAA CAGATCCCGG GTGGCATCCC 
     4561 TGTGACCCCT CCCCAGTGCC TCTCCTGGCC CTGGAAGTTG CCACTCCAGT GCCCACCAGC 
     4621 CTTGTCCTAA TAAAATTAAG TTGCATCATT TTGTCTGACT AGGTGTCCTT CTATAATATT 
     4681 ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG GGGCAAGTTG GGAAGACAAC CTGTAGGGCC 
     4741 TGCGGGGTCT ATTGGGAACC AAGCTGGAGT GCAGTGGCAC AATCTTGGCT CACTGCAATC 
     4801 TCCGCCTCCT GGGTTCAAGC GATTCTCCTG CCTCAGCCTC CCGAGTTGTT GGGATTCCAG 
     4861 GCATGCATGA CCAGGCTCAG CTAATTTTTG TTTTTTTGGT AGAGACGGGG TTTCACCATA 
     4921 TTGGCCAGGC TGGTCTCCAA CTCCTAATCT CAGGTGATCT ACCCACCTTG GCCTCCCAAA 
     4981 TTGCTGGGAT TACAGGCGTG AACCACTGCT CCCTTCCCTG TCCTTCTGAT TTTAAAATAA 
     5041 CTATACCAGC AGGAGGACGT CCAGACACAG CATAGGCTAC CTGGCCATGC CCAACCGGTG 
     5101 GGACATTTGA GTTGCTTGCT TGGCACTGTC CTCTCATGCG TTGGGTCCAC TCAGTAGATG 
     5161 CCTGTTGAAT TGGGTACGCG GCCAGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT 
     5221 ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA 
     5281 AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC 
     5341 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA 
     5401 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC 
     5461 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC 
     5521 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA 
     5581 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC 
     5641 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG 
     5701 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG 
     5761 AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG 
     5821 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA 
     5881 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA 
     5941 CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT 
     6001 CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA 
     6061 GTAACCTGAG GCTATGGCAG GGCCTGCCGC CCCGACGTTG GCTGCGAGCC CTGGGCCTTC 
     6121 ACCCGAACTT GGGGGGTGGG GTGGGGAAAA GGAAGAAACG CGGGCGTATT GGCCCCAATG 
     6181 GGGTCTCGGT GGGGTATCGA CAGAGTGCCA GCCCTGGGAC CGAACCCCGC GTTTATGAAC 
     6241 AAACGACCCA ACACCGTGCG TTTTATTCTG TCTTTTTATT GCCGTCATAG CGCGGGTTCC 
     6301 TTCCGGTATT GTCTCCTTCC GTGTTTCAGT TAGCCTCCCC CTAGGGTGGG CGAAGAACTC 
     6361 CAGCATGAGA TCCCCGCGCT GGAGGATCAT CCAGCCGGCG TCCCGGAAAA CGATTCCGAA 
     6421 GCCCAACCTT TCATAGAAGG CGGCGGTGGA ATCGAAATCT CGTGATGGCA GGTTGGGCGT 
     6481 CGCTTGGTCG GTCATTTCGA ACCCCAGAGT CCCGCTCAGA AGAACTCGTC AAGAAGGCGA 
     6541 TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG GCGATACCGT AAAGCACGAG GAAGCGGTCA 
     6601 GCCCATTCGC CGCCAAGCTC TTCAGCAATA TCACGGGTAG CCAACGCTAT GTCCTGATAG 
     6661 CGATCCGCCA CACCCAGCCG GCCACAGTCG ATGAATCCAG AAAAGCGGCC ATTTTCCACC 
     6721 ATGATATTCG GCAAGCAGGC ATCGCCATGG GTCACGACGA GATCCTCGCC GTCGGGCATG 
     6781 CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT GGCGCGAGCC CCTGATGCTC TTCGTCCAGA 
     6841 TCATCCTGAT CGACAAGACC GGCTTCCATC CGAGTACGTG CTCGCTCGAT GCGATGTTTC 
     6901 GCTTGGTGGT CGAATGGGCA GGTAGCCGGA TCAAGCGTAT GCAGCCGCCG CATTGCATCA 
     6961 GCCATGATGG ATACTTTCTC GGCAGGAGCA AGGTGAGATG ACAGGAGATC CTGCCCCGGC 
     7021 ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC GCTTCAGTGA CAACGTCGAG CACAGCTGCG 
     7081 CAAGGAACGC CCGTCGTGGC CAGCCACGAT AGCCGCGCTG CCTCGTCTTG CAGTTCATTC 
     7141 AGGGCACCGG ACAGGTCGGT CTTGACAAAA AGAACCGGGC GCCCCTGCGC TGACAGCCGG 
     7201 AACACGGCGG CATCAGAGCA GCCGATTGTC TGTTGTGCCC AGTCATAGCC GAATAGCCTC 
     7261 TCCACCCAAG CGGCCGGAGA ACCTGCGTGC AATCCATCTT GTTCAATCAT GCGAAACGAT 
     7321 CCTCATCCTG TCTCTTGATC GATCTTTGCA AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA 
     7381 CTACTTCTGG AATAGCTCAG AGGCCGAGGC GGCCTCGGCC TCTGCATAAA TAAAAAAAAT 
     7441 TAGTCAGCCA TGGGGCGGAG AATGGGCGGA ACTGGGCGGA GTTAGGGGCG GGATGGGCGG 
     7501 AGTTAGGGGC GGGACTATGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     7561 TGCTGGGGAG CCTGGGGACT TTCCACACCT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     7621 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CCTAACTGAC ACACATTCCA 
     7681 CAGCTGGTTC TTTCCGCCTC AGGACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG 
     7741 GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG 
     7801 GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGCGC CCTGTAGCGG CGCATTAAGC 
     7861 GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC 
     7921 GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT 
     7981 CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA 
     8041 AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC 
     8101 CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA 
     8161 CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT 
     8221 TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTT
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