LOCUS       MR211660                8247 bp    DNA     circular  SYN 
DEFINITION  Sema5b (Myc-DDK-tagged) - Mouse sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic
ACCESSION   BC052397
VERSION     BC052397.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4404..4495 
                     /label="Myc-DDK"  
     misc_feature    4525..5110 
                     /label="PolyA signal"  
     misc_feature    4582..4601 
                     /label="Restriction Enzyme"  
     misc_feature    5386..6058 
                     /label="ColE1"  
     misc_feature    6488..7282 
                     /label="Kan/Neo"  
     misc_feature    7317..7619 
                     /label="SV40 ori"  
     misc_feature    7705..8182 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4386..4392 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4386 
                     /label="Sema5b"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGTAGTCCCA GGACCCCTGG CTCTCTCGCT GTTGCTGTCC AGCCTCACCC 
     1081 TGCTGGTGTC CCACCTCTCC AGCTCCCAGG ACATTGCCAG TGAGTCCAGC AGTGAGCAAC 
     1141 AGATGTGCAC GCGGAGGGAG CACCCCATCG TGGCCTTTGA AGATCTGAAG CCGTGGGTCT 
     1201 TTAACTTCAC CTACCCTGGA GTCCGGGATT TCTCCCAGCT TGCTCTTGAT CCCTCGAGGA 
     1261 ATCAGCTCAT CGTGGGAGCC AGGAACTACC TCTTCAGACT CAGCCTCGCC AATGTCTCCC 
     1321 TCCTTCAGGC TACAGAGTGG GCCTCCAGTG AGGACACGCG CCGCTCCTGC CAGAGCAAAG 
     1381 GGAAAACGGA GGAGGAGTGT CAGAACTATG TACGAGTCCT GATTGTTTCC GGCCGGAAGG 
     1441 TGTTCATGTG CGGTACCAAT GCCTTTTCCC CAGTGTGCTC CAGCAGACAG GTAGGGAACC 
     1501 TCAGCCGGAC TATTGAGAAG ATCAACGGTG TGGCCCGGTG CCCCTATGAC CCACGCCACA 
     1561 ACTCTACAGC CGTCATCTCC TCTCAGGGGG AGCTCTATGC AGCCACAGTC ATTGACTTCT 
     1621 CCGGTCGGGA CCCAGCCATC TACCGCAGCC TGGGCAGTGG GCCACCGCTT CGCACTGCCC 
     1681 AGTATAACTC CAAATGGCTC AATGAGCCAA ATTTTGTGGC AGCCTTTGAC ATCGGGCTGT 
     1741 TTGCGTATTT CTTCCTTCGG GAGAATGCCG TGGAGCATGA TTGTGGGCGC ACTGTGTACT 
     1801 CTCGGGTGGC CCGGGTGTGC AAGAATGATG TAGGTGGCCG TTTCCTGCTG GAGGACACGT 
     1861 GGACCACATT CATGAAGGCC CGGCTCAACT GCTCCCGCCC GGGAGAGGTC CCCTTCTACT 
     1921 ATAATGAGCT GCAGAGTGCC TTCCATCTGC CCGAGCAGGA CCTCATCTAT GGCGTCTTCA 
     1981 CCACTAACGT AAACAGCATT GCGGCTTCTG CTGTCTGCGC CTTCAACCTC AGTGCCATCT 
     2041 CCAAGGCTTT CAATGGCCCA TTTCGTTACC AGGAAAACCC CAGGGCTGCC TGGCTCCCAA 
     2101 TCGCCAATCC CATCCCCAAT TTCCAGTGTG GCACTCTGCC GGAGACTGGC CCCAACGAGA 
     2161 ACCTCACGGA GCGCAGCCTG CAGGACGCAC AGCGGCTCTT CCTGATGAGC GAAGCTGTGC 
     2221 AGCCAGTGAC ACCAGAGCCC TGTGTCACCC AGGACAGCGT CCGCTTCTCA CATCTCGTGG 
     2281 TAGACCTTGT GCAAGCTAAG GACACGCTCT ATCACGTACT CTACATAGGC ACGGAGTCGG 
     2341 GTACCATCCT GAAAGCGCTG TCCACGGCCA GCCGCAGCCT CCGTGGCTGC TACCTGGAGG 
     2401 AGCTACATGT GCTGCCTCCT GGGCGCCTTG AACCTCTGCG GAGCCTGCGC ATCCTGCACA 
     2461 GCGCGCGTGC GCTCTTCGTG GGATTGAGCG ACAGGGTGCT GCGGGTCCCA CTGGAGAGGT 
     2521 GTTCGGCCTA TCATAGCCAG GGGGCATGCC TGGGAGCACG GGACCCATAC TGCGGCTGGG 
     2581 ATGGGAAGCG GCAACTTTGC AGCACGCTTG AAGACAGTTC CAACATGAGC CTGTGGATCC 
     2641 AGAACATCAC AACCTGTCCT GTACGAAATG TGACACGGGA TGGGGGCTTC GGCCCATGGT 
     2701 CACCATGGAA ACCGTGTGAG CACTTAGATG GAGACAACTC GGGTTCTTGC CTGTGCCGGG 
     2761 CCAGATCCTG TGACTCCCCA AGGCCTCGCT GTGGGGGCCT CGAGTGCCTG GGGCCATCCA 
     2821 TCCATATTGC CAATTGCTCC AGGAATGGGG CGTGGACCGC ATGGTCATCG TGGGCTCAGT 
     2881 GCAGCACGTC CTGTGGGATC GGCTTCCAGG TCCGTCAGCG AAGTTGCAGC AACCCGGCGC 
     2941 CCCGCCATGG GGGCCGCATT TGCGTGGGCA AGAGCCGGGA GGAGCGGTTC TGTAATGAAA 
     3001 ATACACCTTG CCCAGTGCCC ATCTTCTGGG CTTCCTGGGG TTCCTGGAGC AAGTGCAGCA 
     3061 ACAACTGTGG AGGCGGCGTG CAGTCGCGAC GCCGTTCTTG CGAGAATGGC AACTCGTGCC 
     3121 CGGGTTGCGG CGTGGTGAGG TCGCGGGGCT GGGGCGCGGG GAGCGGGGAG ACCTACAGTC 
     3181 CAGGCGGGGT TGGGGGGTCT GAAGCCACCC TCACCCCGAA GGAGTTCAAG ACCTGCAACC 
     3241 CCGAGGCTTG CCCGGAAGTG CGACGCAACA CACCCTGGAC GCCCTGGCTG CCCGTGAACG 
     3301 TGACCCAGGG TGGAGCGCGC CAGGAGCAGC GATTCCGCTT CACCTGCCGC GCGCCGCTGC 
     3361 CGGACCCGCA CGGTCTGCAG TTCGGCAAGA GGAGGACAGA GACCAGGACT TGCCCGGCAG 
     3421 ACGGCACCGG AGCCTGCGAC ACCGACGCCC TGGTGGAGGA TCTCCTGCGC AGCGGGAGCA 
     3481 CGTCACCACA CACTCTGAAC GGAGGCTGGG CCACCTGGGG CCCGTGGTCA TCCTGCTCCC 
     3541 GGGACTGCGA GCTGGGCTTC CGCGTCCGCA AGAGAACTTG TACCAACCCG GAGCCTCGCA 
     3601 ACGGGGGCTT GCCCTGCGTG GGAGACGCTG CGGAGTACCA AGACTGCAAC CCACAGGCTT 
     3661 GCCCAGTGCG GGGTGCTTGG TCCTGCTGGA CCGCATGGTC CCAGTGCTCA GCATCCTGCG 
     3721 GTGGTGGCCA CTATCAACGC ACCCGTTCCT GCACCAGCCC CGCCCCATCC CCAGGTGAGG 
     3781 ACATCTGCCT CGGCCTGCAC ACGGAGGAGG CCCTATGTTC AACACAGGCC TGCCCAGAAG 
     3841 GCTGGTCACT GTGGTCTGAG TGGGGTGTCT GCACTGAGGA TGGGGCCCAG AGCCGGAGCC 
     3901 GGAGCTGTGA GGAGCTTCTC CCAGGACCAG GTGCCTGTGT TGGCAACAGC AGCCAGAGCC 
     3961 GGCCCTGCCC CTACAGTGAG ATTCCTGTCA TCCTACCTGC TTCCAGTGTG GAGGAGACCA 
     4021 CCAGCTGTGG AGGGTTCAAT CTCATCCACC TGATAGTCAC TGGTGTGTCC TGCTTCCTGG 
     4081 TTTCTGGGCT CTTGACCTTG GCAGTGTACC TGTCTTGCCA GCACTGCCAG CGCCAGTCTC 
     4141 AGGAGTCCAC GCTTGTCCAT CCTGCCACAC CTAACCACTT GCACTACAAG GGTGGGGGCA 
     4201 CCCCCAAGAA TGAGAAGTAC ACCCCTATGG AATTCAAGAC ACTGAACAAG AACAACTTAA 
     4261 TCCCTGATGA CAGAGCCAAC TTCTACCCAC TGCAGCAGAC CAATGTGTAT ACAACCACGT 
     4321 ACTACCCCAG CCCACTGAAC AAGCCCAGCT TCCGGCCTGA GGCCTCACCT GGACAGCGCT 
     4381 GTTTCCCCAA CAGCACGCGT ACGCGGCCGC TCGAGCAGAA ACTCATCTCA GAAGAGGATC 
     4441 TGGCAGCAAA TGATATCCTG GATTACAAGG ATGACGACGA TAAGGTTTAA ACGGCCGGCC 
     4501 GCGGTCATAG CTGTTTCCTG AACAGATCCC GGGTGGCATC CCTGTGACCC CTCCCCAGTG 
     4561 CCTCTCCTGG CCCTGGAAGT TGCCACTCCA GTGCCCACCA GCCTTGTCCT AATAAAATTA 
     4621 AGTTGCATCA TTTTGTCTGA CTAGGTGTCC TTCTATAATA TTATGGGGTG GAGGGGGGTG 
     4681 GTATGGAGCA AGGGGCAAGT TGGGAAGACA ACCTGTAGGG CCTGCGGGGT CTATTGGGAA 
     4741 CCAAGCTGGA GTGCAGTGGC ACAATCTTGG CTCACTGCAA TCTCCGCCTC CTGGGTTCAA 
     4801 GCGATTCTCC TGCCTCAGCC TCCCGAGTTG TTGGGATTCC AGGCATGCAT GACCAGGCTC 
     4861 AGCTAATTTT TGTTTTTTTG GTAGAGACGG GGTTTCACCA TATTGGCCAG GCTGGTCTCC 
     4921 AACTCCTAAT CTCAGGTGAT CTACCCACCT TGGCCTCCCA AATTGCTGGG ATTACAGGCG 
     4981 TGAACCACTG CTCCCTTCCC TGTCCTTCTG ATTTTAAAAT AACTATACCA GCAGGAGGAC 
     5041 GTCCAGACAC AGCATAGGCT ACCTGGCCAT GCCCAACCGG TGGGACATTT GAGTTGCTTG 
     5101 CTTGGCACTG TCCTCTCATG CGTTGGGTCC ACTCAGTAGA TGCCTGTTGA ATTGGGTACG 
     5161 CGGCCAGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC 
     5221 AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA 
     5281 AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA 
     5341 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC 
     5401 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC 
     5461 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG 
     5521 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA 
     5581 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC 
     5641 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC 
     5701 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG 
     5761 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA 
     5821 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA 
     5881 AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA 
     5941 CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT 
     6001 AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAACCTG AGGCTATGGC 
     6061 AGGGCCTGCC GCCCCGACGT TGGCTGCGAG CCCTGGGCCT TCACCCGAAC TTGGGGGGTG 
     6121 GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA TTGGCCCCAA TGGGGTCTCG GTGGGGTATC 
     6181 GACAGAGTGC CAGCCCTGGG ACCGAACCCC GCGTTTATGA ACAAACGACC CAACACCGTG 
     6241 CGTTTTATTC TGTCTTTTTA TTGCCGTCAT AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT 
     6301 CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG GGCGAAGAAC TCCAGCATGA GATCCCCGCG 
     6361 CTGGAGGATC ATCCAGCCGG CGTCCCGGAA AACGATTCCG AAGCCCAACC TTTCATAGAA 
     6421 GGCGGCGGTG GAATCGAAAT CTCGTGATGG CAGGTTGGGC GTCGCTTGGT CGGTCATTTC 
     6481 GAACCCCAGA GTCCCGCTCA GAAGAACTCG TCAAGAAGGC GATAGAAGGC GATGCGCTGC 
     6541 GAATCGGGAG CGGCGATACC GTAAAGCACG AGGAAGCGGT CAGCCCATTC GCCGCCAAGC 
     6601 TCTTCAGCAA TATCACGGGT AGCCAACGCT ATGTCCTGAT AGCGATCCGC CACACCCAGC 
     6661 CGGCCACAGT CGATGAATCC AGAAAAGCGG CCATTTTCCA CCATGATATT CGGCAAGCAG 
     6721 GCATCGCCAT GGGTCACGAC GAGATCCTCG CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG 
     6781 AACAGTTCGG CTGGCGCGAG CCCCTGATGC TCTTCGTCCA GATCATCCTG ATCGACAAGA 
     6841 CCGGCTTCCA TCCGAGTACG TGCTCGCTCG ATGCGATGTT TCGCTTGGTG GTCGAATGGG 
     6901 CAGGTAGCCG GATCAAGCGT ATGCAGCCGC CGCATTGCAT CAGCCATGAT GGATACTTTC 
     6961 TCGGCAGGAG CAAGGTGAGA TGACAGGAGA TCCTGCCCCG GCACTTCGCC CAATAGCAGC 
     7021 CAGTCCCTTC CCGCTTCAGT GACAACGTCG AGCACAGCTG CGCAAGGAAC GCCCGTCGTG 
     7081 GCCAGCCACG ATAGCCGCGC TGCCTCGTCT TGCAGTTCAT TCAGGGCACC GGACAGGTCG 
     7141 GTCTTGACAA AAAGAACCGG GCGCCCCTGC GCTGACAGCC GGAACACGGC GGCATCAGAG 
     7201 CAGCCGATTG TCTGTTGTGC CCAGTCATAG CCGAATAGCC TCTCCACCCA AGCGGCCGGA 
     7261 GAACCTGCGT GCAATCCATC TTGTTCAATC ATGCGAAACG ATCCTCATCC TGTCTCTTGA 
     7321 TCGATCTTTG CAAAAGCCTA GGCCTCCAAA AAAGCCTCCT CACTACTTCT GGAATAGCTC 
     7381 AGAGGCCGAG GCGGCCTCGG CCTCTGCATA AATAAAAAAA ATTAGTCAGC CATGGGGCGG 
     7441 AGAATGGGCG GAACTGGGCG GAGTTAGGGG CGGGATGGGC GGAGTTAGGG GCGGGACTAT 
     7501 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     7561 CTTTCCACAC CTGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     7621 GGAGCCTGGG GACTTTCCAC ACCCTAACTG ACACACATTC CACAGCTGGT TCTTTCCGCC 
     7681 TCAGGACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC 
     7741 GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC 
     7801 CGAAAAGTGC CACCTGACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT 
     7861 ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC 
     7921 CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT 
     7981 TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT 
     8041 GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC 
     8101 ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC 
     8161 TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG 
     8221 ATTTAACAAA AATTTAACGC GAATTTT
//