LOCUS       MR211659                8244 bp    DNA     circular  SYN 
DEFINITION  Anln (Myc-DDK-tagged) - Mouse anillin, actin binding protein (Anln)
ACCESSION   NM_028390
VERSION     NM_028390.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4401..4492 
                     /label="Myc-DDK"  
     misc_feature    4522..5107 
                     /label="PolyA signal"  
     misc_feature    4579..4598 
                     /label="Restriction Enzyme"  
     misc_feature    5383..6055 
                     /label="ColE1"  
     misc_feature    6485..7279 
                     /label="Kan/Neo"  
     misc_feature    7314..7616 
                     /label="SV40 ori"  
     misc_feature    7702..8179 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4383..4389 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4383 
                     /label="Anln"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGACCCGTTT ACCGAGAAGT TGCTAGAACG AACTCGTGCC AGACGAGAGA 
     1081 ATCTTCAGAG AAAAATGGCT GAGAGGCCTA CGGCAGTAGC GAGATCTGCC CCGCATGCGA 
     1141 AGAGAGGCAG AGAGCCACTT TCAGAAGCAA GTAATCAGCA GCAGCCCCTA CCAGGGGGCG 
     1201 AAGAAAAATC TTGTACAAAA CCATCACCAT CAAAAAAACG TTGTTCTGAC AAAATTGAAG 
     1261 TGGGAGCTCC GGACTTAGAA AATACAGAAC CTATTGATGT TGCAAAGCCC TGTTCTCCGA 
     1321 TGCCTGCACC CCGGCAGGCG AAGCCCCCAG CACCAGCTGC CATCAGCGAG TCTGTGGCTG 
     1381 CCCCAGCAGC CCTGCTCAGC GCGGACAGAG GGCTGAACTC AGGATCCGAA GCATCTGCAA 
     1441 CCTCCTCAGT TAAAACTCGA ATGCAAAGGC TTGCTGAGCA GCGGCGCCAT TGGGATAGTG 
     1501 ATCTCACAGA TGATGTATCA GAAAGTTCAT ACTTTGCACC AGTGCCAACT GAGGACAAGG 
     1561 CTGCCTCACC TTCTAAGCCA CCCATTTCAA ATGCCTCAGC TACTCCAGTT GGGAGAAGGG 
     1621 GCCGTCTGGC CAACCTTGCT GCAACGATTT GCTCCTGGGA AGATGATGTA AGCCACTCAT 
     1681 CTGCAAAGCA AAATAGTGTG CAAGAACAGC CTGGTACCGC TTGTTTATCC AAATCTTCCT 
     1741 CTGCAAGTGG AGCATCTGCT AGCATCAATA GCAGCAGTGT TCAGCAGGAA GCTACATGCT 
     1801 GTTCCCCAAG GGACGGCAAT GCCTCTGTCA GGAAAGACCC ATCTTCAAAT GCTGCCCATG 
     1861 GACCTTTGCT TAGCGCCTCA GTGTCCAGCT CTGTGAAAGC GTCTTCCCCT GTGACAGCTG 
     1921 CTACCTTCAT CACTGAAAAC CGTGAGGCAC AAAATCCTGA GCTACTTCAC AAAACTGCTA 
     1981 GTCCTCTGAA AACAGAGGCG CGGAAACCAT GTGAGAAGCC AACTTTGTCC CAGGGAGCTC 
     2041 AGCCCAAAGA GGAGGCTAAC AGAGAAGTTT GTCTACAGTC ACAATCCAAG GACAAACTTG 
     2101 CAACACCAGG AGGAAGAGGA ATTAAGCCTT TCCTGGAACG CTTTGGAGAG CGTTGTCAAG 
     2161 AACACAGTAA AGAAAGTCCG TCTTATAGAG CATCTCATAA AACCCCAAAT ATCACTCCAA 
     2221 ATACAAAAGC CATCCAGGAA AGATTATTCA AACAAAACAC ATGCTCGTCT ACTACCCATT 
     2281 TAGCACAGCA GCTCAAACAG GAACGTGAAA AAGAACTGGC ATGTCTTCGT GGTCGACTTG 
     2341 ACAAGGGCAA TTTATGGAGT GCAGAAAAGA ATGAAAAGTC AAGAAGCAAG CATCTAGAAA 
     2401 CCAAACAGGA AGTTCACTGT CAGAACACTC CACTCAAGAA ACATCAAACT GTCGCAAGCA 
     2461 CCCCATTGAC TTCTGTAACA GATAAGGTGG CTGAAAATGA ACCAGCAGTG AAGCTTTCTA 
     2521 GCACAGAGCC TGCAGGTTCC ACTGAAAGCG AAATGACAAA GTCCAGCCCT TTGAAAATCA 
     2581 CGTTGTTTTT AGAAGAAGAA AAATCCTTAA AAGTAGCATC AGACCTGGAG GTTGAGCAGA 
     2641 ACACTGAAGC AGTGCGTGAG GTTGAGATGA GTGTGGACGA TGAGGACATC AATAGCTCCA 
     2701 GAGTCATTAA CGACATCTTC AGTGACGTCC TAGAGGAAGG GGAGCTGGAT GTGGAAAAGA 
     2761 GCCAAGAGGA GATGGACCAA GTGGGAGCAG AAAACAGTGA GGAGCAGGAA GATGCGCTCA 
     2821 ATATCTCTTC AATGTCTTTA CTTGCTCCGC TAGCTCAGAC GGTCGGTGTG GTGAGCCTAG 
     2881 AGAATGTAAT TTCTTCACCT CCGTCGGAAT TGAGAGACTC TAACCTAAGC GCTGCAAGTC 
     2941 CTAAGCCCGG GAAATTCCAG AGAACCCGCG TCCCTCGCGC CGAATCTGCC GATAGCCTCG 
     3001 GTTCTGAGGA CCGGGACCTT CTCTATAGCA TTGATGCATA TAGGTCTCAA AGATTCAAAG 
     3061 AAACAGAACG CCCTTCCATA AAGCAAGTGA TTGTTCGAAA GGAAGATGTT ACTTCAAAGT 
     3121 TGGGTGAAAA GAAAAACGTA TTTTCTGGTC AAGTTAATAT CAAACAAAAA ATGCAGGAGC 
     3181 TCAATAATGA CATAAATTTG CAGCAGACAG TGATCTATCA GGCCAGTCAG GCTCTCAACT 
     3241 GCTGTGTGGA TGAAGAACAC GGGAAAGGAT CCTTGGAAGA AGCTGAAGCA GAAAGACTTC 
     3301 TTCTGATTGC AACTGAGAAA AGAGCACTTC TGATTGATGA GCTGAATAAG CTGAAGAGTG 
     3361 AAGGACCTCA GAGGAGAAAC AAGACCAGTG TCATATCCCA GAGTGAATTT GCTCCATCTA 
     3421 AAGGGTCAGT CACTCTGTCA GAAATCTGCT TGCCTCTGAA GGCAGATTTT GTCTGCAGCA 
     3481 CTGCGCAAAA AACAGATGCA TCAAATTATT ACTACTTAAT TATGCTAAAA GCTGGGGCTG 
     3541 AGCAGATGGT CGCCACTCCA TTAGCAAGTA CTGCAAACTC TCTCAGTGGT GACGCTCTGA 
     3601 CATTTCCTAC TACATTTACT CTGCATGATG TTTCCAATGA CTTTGAAATA AACATTGAAG 
     3661 TTTACAGCCT GGTACAAAAG AAAGATTCCT TGGGCCCCGA TAAGAAGAAG AAAGCCTCCA 
     3721 AGTCCAAGGC TATTACTCCA AAGAGACTCC TCACATCTAT AACTTCAAAA AGCAGCCTTC 
     3781 ATTCTTCAGT TATGGCCAGT CCCGGAGGTC TCGGTGCTGT GCGTACCAGC AACTTTACCC 
     3841 TAGTTGGATC TCACACACTC TCCTTATCTT CTGTTGGAGA CACTAAGTTT GCTTTGGACA 
     3901 AGGTACCTTT TTTGTCTCCG TTGGAAGGTC ACATCTGTTT AAAAATAAGC TGTCAAGTGA 
     3961 ATTCAGCTGT TGAGGAAAAG GGTTTCCTTA CCATATTTGA AGATGTTAGT GGCTTTGGTG 
     4021 CCTGGCACCG AAGATGGTGT GTTCTCTCTG GCAACTGTAT CTCTTACTGG ACTTACCCAG 
     4081 ATGATGAGAG GCGAAAGAAT CCCATAGGAA GGATAAATCT GGCCAATTGT ATCAGTCATC 
     4141 AGATAGAACC AGCCAACAGA GAATTTTGTG CAAGACGCAA CACTCTGGAA TTGATTACTG 
     4201 TCCGACCACA AAGAGAAGAC GATCGAGAAA CTCTTGTCAG CCAATGTAGA GACACACTCT 
     4261 GTGTCACCAA GAACTGGCTC TCTGCAGATA CTAAAGAAGA GCGGGATCTC TGGATGCAGA 
     4321 AACTCAACCA GGTCATTGTT GATATTCGCC TCTGGCAGCC TGATGCATGC TACAAGCCTG 
     4381 TTGGGAAGCC TACGCGTACG CGGCCGCTCG AGCAGAAACT CATCTCAGAA GAGGATCTGG 
     4441 CAGCAAATGA TATCCTGGAT TACAAGGATG ACGACGATAA GGTTTAAACG GCCGGCCGCG 
     4501 GTCATAGCTG TTTCCTGAAC AGATCCCGGG TGGCATCCCT GTGACCCCTC CCCAGTGCCT 
     4561 CTCCTGGCCC TGGAAGTTGC CACTCCAGTG CCCACCAGCC TTGTCCTAAT AAAATTAAGT 
     4621 TGCATCATTT TGTCTGACTA GGTGTCCTTC TATAATATTA TGGGGTGGAG GGGGGTGGTA 
     4681 TGGAGCAAGG GGCAAGTTGG GAAGACAACC TGTAGGGCCT GCGGGGTCTA TTGGGAACCA 
     4741 AGCTGGAGTG CAGTGGCACA ATCTTGGCTC ACTGCAATCT CCGCCTCCTG GGTTCAAGCG 
     4801 ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG GGATTCCAGG CATGCATGAC CAGGCTCAGC 
     4861 TAATTTTTGT TTTTTTGGTA GAGACGGGGT TTCACCATAT TGGCCAGGCT GGTCTCCAAC 
     4921 TCCTAATCTC AGGTGATCTA CCCACCTTGG CCTCCCAAAT TGCTGGGATT ACAGGCGTGA 
     4981 ACCACTGCTC CCTTCCCTGT CCTTCTGATT TTAAAATAAC TATACCAGCA GGAGGACGTC 
     5041 CAGACACAGC ATAGGCTACC TGGCCATGCC CAACCGGTGG GACATTTGAG TTGCTTGCTT 
     5101 GGCACTGTCC TCTCATGCGT TGGGTCCACT CAGTAGATGC CTGTTGAATT GGGTACGCGG 
     5161 CCAGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG 
     5221 GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA 
     5281 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG 
     5341 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC 
     5401 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC 
     5461 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC 
     5521 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG 
     5581 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC 
     5641 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA 
     5701 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC 
     5761 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC 
     5821 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG 
     5881 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC 
     5941 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA 
     6001 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAACCTGAGG CTATGGCAGG 
     6061 GCCTGCCGCC CCGACGTTGG CTGCGAGCCC TGGGCCTTCA CCCGAACTTG GGGGGTGGGG 
     6121 TGGGGAAAAG GAAGAAACGC GGGCGTATTG GCCCCAATGG GGTCTCGGTG GGGTATCGAC 
     6181 AGAGTGCCAG CCCTGGGACC GAACCCCGCG TTTATGAACA AACGACCCAA CACCGTGCGT 
     6241 TTTATTCTGT CTTTTTATTG CCGTCATAGC GCGGGTTCCT TCCGGTATTG TCTCCTTCCG 
     6301 TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC GAAGAACTCC AGCATGAGAT CCCCGCGCTG 
     6361 GAGGATCATC CAGCCGGCGT CCCGGAAAAC GATTCCGAAG CCCAACCTTT CATAGAAGGC 
     6421 GGCGGTGGAA TCGAAATCTC GTGATGGCAG GTTGGGCGTC GCTTGGTCGG TCATTTCGAA 
     6481 CCCCAGAGTC CCGCTCAGAA GAACTCGTCA AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA 
     6541 TCGGGAGCGG CGATACCGTA AAGCACGAGG AAGCGGTCAG CCCATTCGCC GCCAAGCTCT 
     6601 TCAGCAATAT CACGGGTAGC CAACGCTATG TCCTGATAGC GATCCGCCAC ACCCAGCCGG 
     6661 CCACAGTCGA TGAATCCAGA AAAGCGGCCA TTTTCCACCA TGATATTCGG CAAGCAGGCA 
     6721 TCGCCATGGG TCACGACGAG ATCCTCGCCG TCGGGCATGC TCGCCTTGAG CCTGGCGAAC 
     6781 AGTTCGGCTG GCGCGAGCCC CTGATGCTCT TCGTCCAGAT CATCCTGATC GACAAGACCG 
     6841 GCTTCCATCC GAGTACGTGC TCGCTCGATG CGATGTTTCG CTTGGTGGTC GAATGGGCAG 
     6901 GTAGCCGGAT CAAGCGTATG CAGCCGCCGC ATTGCATCAG CCATGATGGA TACTTTCTCG 
     6961 GCAGGAGCAA GGTGAGATGA CAGGAGATCC TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG 
     7021 TCCCTTCCCG CTTCAGTGAC AACGTCGAGC ACAGCTGCGC AAGGAACGCC CGTCGTGGCC 
     7081 AGCCACGATA GCCGCGCTGC CTCGTCTTGC AGTTCATTCA GGGCACCGGA CAGGTCGGTC 
     7141 TTGACAAAAA GAACCGGGCG CCCCTGCGCT GACAGCCGGA ACACGGCGGC ATCAGAGCAG 
     7201 CCGATTGTCT GTTGTGCCCA GTCATAGCCG AATAGCCTCT CCACCCAAGC GGCCGGAGAA 
     7261 CCTGCGTGCA ATCCATCTTG TTCAATCATG CGAAACGATC CTCATCCTGT CTCTTGATCG 
     7321 ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA GCCTCCTCAC TACTTCTGGA ATAGCTCAGA 
     7381 GGCCGAGGCG GCCTCGGCCT CTGCATAAAT AAAAAAAATT AGTCAGCCAT GGGGCGGAGA 
     7441 ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG GATGGGCGGA GTTAGGGGCG GGACTATGGT 
     7501 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     7561 TCCACACCTG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     7621 GCCTGGGGAC TTTCCACACC CTAACTGACA CACATTCCAC AGCTGGTTCT TTCCGCCTCA 
     7681 GGACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA 
     7741 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA 
     7801 AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG 
     7861 CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT 
     7921 TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA 
     7981 GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT 
     8041 TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG 
     8101 TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT 
     8161 TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT 
     8221 TAACAAAAAT TTAACGCGAA TTTT
//