LOCUS       MR211657                8232 bp    DNA     circular  SYN 
DEFINITION  Lrig3 (Myc-DDK-tagged) - Mouse leucine-rich repeats and immunoglobulin-like domains 3 (Lrig3)
ACCESSION   NM_177152
VERSION     NM_177152.5
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4389..4480 
                     /label="Myc-DDK"  
     misc_feature    4510..5095 
                     /label="PolyA signal"  
     misc_feature    4567..4586 
                     /label="Restriction Enzyme"  
     misc_feature    5371..6043 
                     /label="ColE1"  
     misc_feature    6473..7267 
                     /label="Kan/Neo"  
     misc_feature    7302..7604 
                     /label="SV40 ori"  
     misc_feature    7690..8167 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4371..4377 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4371 
                     /label="Lrig3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGTGCTCCC GGACTCCGAG CGGCCACCGC GGCGCTGGGA CTGCTGCTGT 
     1081 GCGCGGGGCT GGGGCGCGCG GGCCCGGCGG GAAGCGGAGG CCACGGGGCT CCCGGGCAGC 
     1141 TCTTGGACGA CGATGCGCAG CGCCCGTGTC CAGCCGCCTG CCACTGCCTC GGGGACCTGC 
     1201 TGGACTGCAG CCGGCGGCGG CTGGTTCGCC TTCCCGATCC TCTCCCGGCC TGGGTCACAC 
     1261 GGCTGGACCT GAGTCACAAC AGATTATCTT TTATCCAGAC GAGTTCCCTG AGCCACCTTC 
     1321 AAAGCCTTCA AGAAGTGAAG CTGAACAACA ATGAATTAGA GACCATTCCA AACCTGGGAT 
     1381 CCATCTCTGC AAATATTAGA CAGCTATCCT TGGCTGGGAA CGCCATCGAC AAAATCCTCC 
     1441 CTGAACAGCT GGAAGCCTTT CAGTCCCTTG AGACTTTGGA CCTCAGCAAC AATAATATTT 
     1501 CAGAACTGAG AACTGCCTTT CCACCCTTAC AACTCAAGTA TCTGTATATC AATAACAACC 
     1561 GAGTGTCGTC CATGGAACCA GGGTATTTTG ACAATTTGGC GAGCACGCTC CTGGTGCTGA 
     1621 AGCTGAACAG GAACCGCATC TCAGCCATCC CTCCCAAGAT GTTTAAACTG CCTCAGCTGC 
     1681 AGCACCTTGA GCTGAACAGA AACAAGATTA AGAACGTGGA TGGACTCACA TTCCAAGGAC 
     1741 TTGGTGCACT GAAGTCTCTG AAAATGCAAA GAAACGGAGT GACAAAGCTG ATGGATGGAG 
     1801 CTTTTTGGGG GCTGAGCAAC ATGGAAGTCC TGCAGCTGGA CCATAACAAC CTAACCGAGA 
     1861 TTACCAAAGG CTGGCTCTAT GGCTTGTTGA TGCTGCGGGA ACTTCATCTG AGCCAGAACG 
     1921 CCATCAACAG GATCAGCCCG GATGCCTGGG AGTTCTGCCA GAAACTCAGT GAGCTAGACC 
     1981 TGACTTTCAA CCACTTATCA AGGCTAGACG ATTCCAGCTT CCTGGGCCTA AGCCTACTAA 
     2041 ATGCCCTCCA CATTGGGAAT AACAAAGTCA GCTACATTGC TGACTGTGCC TTCCGGGGGC 
     2101 TTACCAGTTT AAAGACTTTG GATCTGAGGA ACAATGAAAT CTCATGGACC ATCGAAGACA 
     2161 TGAGTGGGGC ATTCTCTGGG CTGGACAGAT TGAGGCAACT AATTCTCCAG GGAAACCGGA 
     2221 TTCGATCTAT TACCAAAAAG GCCTTCGCTG GTTTGGACAC ATTGGAACAC CTGGACCTGA 
     2281 GTGGCAACGC CATCATGTCT CTACAGAGCA ATGCGTTTTC CCAAATGAAG AAACTTCAGC 
     2341 AGCTGCATTT AAATACGTCG AGCCTTTTGT GTGACTGCCA GCTGAGATGG CTCCCACAGT 
     2401 GGGTGGCGGA GAACAACTTT CAGAGCTTCG TAAATGCCAG TTGTGCCCAC CCTCAGCTGC 
     2461 TGAAAGGGAG GAGCATTTTC ACTGTCAGCC CAGATGGCTT TGTGTGTGAT GATTTTCCCA 
     2521 AACCCCAGAT CACCGTCCAG CCAGAAACGC AGTCGGCAAT AAAGGGCTCC GACGTGAGTT 
     2581 TTACCTGCTC GGCTGCTAGC AGCAGTGACT CCCCTATGAC GTTCGCTTGG AAGAAAGACA 
     2641 ACGAAGCGCT TCAGGATGCA GAGATGGAGA ACTACGCACA CCTGCGGGCC CAGGGAGGCG 
     2701 AGCTGATGGA GTATACCACC ATCCTGAGGC TGCGCAATGT GGAATTCACC AGCGAAGGGA 
     2761 AGTACCAGTG TGTCATCTCC AACCACTTCG GTTCGTCATA CTCCGTCAAA GCCAAGCTCA 
     2821 CCATCAACAT GTTGCCTTCC TTTACCAAGA CCCCCATGGA CCTCACCATC CGTGCCGGGG 
     2881 CCATGGCCCG CCTGGAGTGT GCTGCCGTGG GGCACCCAGC CCCACAGATA GCCTGGCAGA 
     2941 AGGATGGAGG CACGGACTTC CCGGCCGCCC GAGAGAGACG CATGCACGTG ATGCCCGAGG 
     3001 ATGACGTCTT CTTCATCGTG GACGTGAAGA TCGAGGACAT CGGGGTTTAC AGCTGCACAG 
     3061 CTCAGAACAG TGCAGGGAGC GTCTCTGCAA ACGCAACCCT GACTGTGCTA GAGACGCCGT 
     3121 CATTTTTGCG GCCCCTGTTA GACAGAACCG TCACCAAGGG AGAAACAGCG GTCCTGCAGT 
     3181 GCATTGCTGG AGGGAGCCCG CCCCCTCGGC TGAACTGGAC GAAAGATGAC AGCCCTTTGG 
     3241 TGGTGACCGA GAGGCACTTT TTTGCAGCAG GCAACCAGCT GCTGATCATC GTGGACTCGG 
     3301 ACGTCAGCGA CGCCGGGAAA TACACGTGTG AGATGTCCAA TACCCTTGGC ACCGAGAGAG 
     3361 GCAACGTTCG CCTCAGTGTG ATCCCTACTC CGACCTGCGA CTCCCCTCAC ATGACTGCCC 
     3421 CATCGCTGGA CGGCGATGGA TGGGCCACCG TGGGCGTGGT GATCATAGCT GTGGTTTGCT 
     3481 GTGTGGTGGG CACATCCCTC GTGTGGGTGG TCATCATATA CCACACAAGG CGGAGGAACG 
     3541 AAGACTGCAG CATTACCAAC ACAGATGAGA CCAACTTGCC GGCTGACATC CCTAGTTATC 
     3601 TATCATCTCA GGGAACACTA GCTGACAGGC AGGACGGGTA CATCTCCTCA GAAAGCGGAA 
     3661 GTCACCACCA GTTCGTGACT TCTTCGGGAG GCGGATTTTT CTTACCACAA CACGACGGTG 
     3721 CTGGGACCTG CCATTTTGAC GACAGCAGTG AGGCTGATGT GGAAGCCGCT TCTGACCCGT 
     3781 TTCTTTGCCC ATTCGTGGGC TCCACAGGCC CTGTGTATCT ACAGGGGAAT CTGTACAGCC 
     3841 CAGATCCATT TGAAGTTTAT CTTCCAGGTT GCAGCTCTGA CCCGAGGACC GCTTTAATGG 
     3901 ATCACTGTGA GTCCAGCTAC GTGAAGCAGG ACCGCTTCTC CTGTGCTCGT CCCTCAGAGG 
     3961 AACCCTGTGA ACGGAGCCTC AAGAGCATCC CATGGCCACA TTCCAGAAAG CTCACCGACT 
     4021 CCACATATCC TCCGAATGAG GGACACACAG TTCAGACTCT GTGTCTAAAC AAGTCCTCCG 
     4081 TGGATTTCAG TACAGGGCCG GAACCAGGAT CGGCTACTTC AAGTAACTCA TTCATGGGAA 
     4141 CCTTCGGAAA ACCTCTGAGG AGACCACACC TGGATGCCTT TTCAAGCTCC GCACAACCGC 
     4201 CAGACTGTCA GCCAAGGCCC TGTCACGGGA AATCGCTTTC CTCGCCAGAG CTGGACTCTG 
     4261 AATCAGAAGA AAATGACAAA GAAAGGACAG ATTTTCGGGA AGAGAATCAC AGGTGTACTT 
     4321 ATCAACAGAT ATTCCACACT TACAGGACTC CAGATTGCCA GCCTTGTGAT TCAGACACAA 
     4381 CGCGTACGCG GCCGCTCGAG CAGAAACTCA TCTCAGAAGA GGATCTGGCA GCAAATGATA 
     4441 TCCTGGATTA CAAGGATGAC GACGATAAGG TTTAAACGGC CGGCCGCGGT CATAGCTGTT 
     4501 TCCTGAACAG ATCCCGGGTG GCATCCCTGT GACCCCTCCC CAGTGCCTCT CCTGGCCCTG 
     4561 GAAGTTGCCA CTCCAGTGCC CACCAGCCTT GTCCTAATAA AATTAAGTTG CATCATTTTG 
     4621 TCTGACTAGG TGTCCTTCTA TAATATTATG GGGTGGAGGG GGGTGGTATG GAGCAAGGGG 
     4681 CAAGTTGGGA AGACAACCTG TAGGGCCTGC GGGGTCTATT GGGAACCAAG CTGGAGTGCA 
     4741 GTGGCACAAT CTTGGCTCAC TGCAATCTCC GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT 
     4801 CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA TGCATGACCA GGCTCAGCTA ATTTTTGTTT 
     4861 TTTTGGTAGA GACGGGGTTT CACCATATTG GCCAGGCTGG TCTCCAACTC CTAATCTCAG 
     4921 GTGATCTACC CACCTTGGCC TCCCAAATTG CTGGGATTAC AGGCGTGAAC CACTGCTCCC 
     4981 TTCCCTGTCC TTCTGATTTT AAAATAACTA TACCAGCAGG AGGACGTCCA GACACAGCAT 
     5041 AGGCTACCTG GCCATGCCCA ACCGGTGGGA CATTTGAGTT GCTTGCTTGG CACTGTCCTC 
     5101 TCATGCGTTG GGTCCACTCA GTAGATGCCT GTTGAATTGG GTACGCGGCC AGCGGCGAGC 
     5161 GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG 
     5221 AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT 
     5281 GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA 
     5341 GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT 
     5401 CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC 
     5461 GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT 
     5521 TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC 
     5581 CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC 
     5641 CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG 
     5701 GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC 
     5761 AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG 
     5821 CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA 
     5881 TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT 
     5941 TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG 
     6001 TTTTAAATCA ATCTAAAGTA TATATGAGTA ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC 
     6061 GACGTTGGCT GCGAGCCCTG GGCCTTCACC CGAACTTGGG GGGTGGGGTG GGGAAAAGGA 
     6121 AGAAACGCGG GCGTATTGGC CCCAATGGGG TCTCGGTGGG GTATCGACAG AGTGCCAGCC 
     6181 CTGGGACCGA ACCCCGCGTT TATGAACAAA CGACCCAACA CCGTGCGTTT TATTCTGTCT 
     6241 TTTTATTGCC GTCATAGCGC GGGTTCCTTC CGGTATTGTC TCCTTCCGTG TTTCAGTTAG 
     6301 CCTCCCCCTA GGGTGGGCGA AGAACTCCAG CATGAGATCC CCGCGCTGGA GGATCATCCA 
     6361 GCCGGCGTCC CGGAAAACGA TTCCGAAGCC CAACCTTTCA TAGAAGGCGG CGGTGGAATC 
     6421 GAAATCTCGT GATGGCAGGT TGGGCGTCGC TTGGTCGGTC ATTTCGAACC CCAGAGTCCC 
     6481 GCTCAGAAGA ACTCGTCAAG AAGGCGATAG AAGGCGATGC GCTGCGAATC GGGAGCGGCG 
     6541 ATACCGTAAA GCACGAGGAA GCGGTCAGCC CATTCGCCGC CAAGCTCTTC AGCAATATCA 
     6601 CGGGTAGCCA ACGCTATGTC CTGATAGCGA TCCGCCACAC CCAGCCGGCC ACAGTCGATG 
     6661 AATCCAGAAA AGCGGCCATT TTCCACCATG ATATTCGGCA AGCAGGCATC GCCATGGGTC 
     6721 ACGACGAGAT CCTCGCCGTC GGGCATGCTC GCCTTGAGCC TGGCGAACAG TTCGGCTGGC 
     6781 GCGAGCCCCT GATGCTCTTC GTCCAGATCA TCCTGATCGA CAAGACCGGC TTCCATCCGA 
     6841 GTACGTGCTC GCTCGATGCG ATGTTTCGCT TGGTGGTCGA ATGGGCAGGT AGCCGGATCA 
     6901 AGCGTATGCA GCCGCCGCAT TGCATCAGCC ATGATGGATA CTTTCTCGGC AGGAGCAAGG 
     6961 TGAGATGACA GGAGATCCTG CCCCGGCACT TCGCCCAATA GCAGCCAGTC CCTTCCCGCT 
     7021 TCAGTGACAA CGTCGAGCAC AGCTGCGCAA GGAACGCCCG TCGTGGCCAG CCACGATAGC 
     7081 CGCGCTGCCT CGTCTTGCAG TTCATTCAGG GCACCGGACA GGTCGGTCTT GACAAAAAGA 
     7141 ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC ACGGCGGCAT CAGAGCAGCC GATTGTCTGT 
     7201 TGTGCCCAGT CATAGCCGAA TAGCCTCTCC ACCCAAGCGG CCGGAGAACC TGCGTGCAAT 
     7261 CCATCTTGTT CAATCATGCG AAACGATCCT CATCCTGTCT CTTGATCGAT CTTTGCAAAA 
     7321 GCCTAGGCCT CCAAAAAAGC CTCCTCACTA CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC 
     7381 CTCGGCCTCT GCATAAATAA AAAAAATTAG TCAGCCATGG GGCGGAGAAT GGGCGGAACT 
     7441 GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT TAGGGGCGGG ACTATGGTTG CTGACTAATT 
     7501 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCTGGT 
     7561 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     7621 TCCACACCCT AACTGACACA CATTCCACAG CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT 
     7681 TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA 
     7741 TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT 
     7801 GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC 
     7861 GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC 
     7921 ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT 
     7981 AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG 
     8041 CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT 
     8101 GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA 
     8161 TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT 
     8221 AACGCGAATT TT
//