LOCUS       MR211654                8232 bp    DNA     circular  SYN 
DEFINITION  Eif2ak3 (Myc-DDK-tagged) - Mouse eukaryotic translation initiation factor 2 alpha kinase 3 (Eif2ak3)
ACCESSION   NM_010121
VERSION     NM_010121.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4387..4478 
                     /label="Myc-DDK"  
     misc_feature    4508..5093 
                     /label="PolyA signal"  
     misc_feature    4565..4584 
                     /label="Restriction Enzyme"  
     misc_feature    5369..6041 
                     /label="ColE1"  
     misc_feature    6471..7265 
                     /label="Kan/Neo"  
     misc_feature    7300..7602 
                     /label="SV40 ori"  
     misc_feature    7688..8165 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4362..4369 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4362 
                     /label="Eif2ak3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGCGCGCC ACCCGGCCCG GGCCGCGCGC GCTGCTGCTG CTTCTGTTCC 
     1081 TGCTGCTGGG CTGCGCGGCG GGGATCTCGG CGGTCGCGCC CGCCCGCAGT TTGCTTGCTC 
     1141 CCGCGTCGGA GACAGTGTTT GGCTTAGGGG CAGCGGCCGC CCCGACTTCG GCCGCGCGGG 
     1201 TGCCTGCGGT GGCAACGGCC GAAGTGACCG TGGAGGACGC CGAGGCATTG CCGGCTGCCG 
     1261 CTGGCGAACC GGAGTCACGC GCGACGGAGC CCGATGACGA CGTGGAACTG CGGCCTCGCG 
     1321 GCAGGTCCTT GGTAATCATC AGCACTTTAG ATGGACGAAT CGCTGCACTG GATGCCGAGA 
     1381 ATGATGGGAA AAAGCAGTGG GATTTGGACG TGGGGTCTGG TTCCTTGGTT TCATCTAGCC 
     1441 TCAGCAAGCC AGAGGTGTTT GGGAACAAGA TGATCATCCC CTCCCTGGAT GGAGACCTCT 
     1501 TCCAGTGGGA CCGGGACCGA GAGAGCATGG AGGCCGTCCC CTTCACGGTG GAGTCCCTGC 
     1561 TCGAATCTTC CTACAAGTTT GGAGATGATG TTGTTCTGGT TGGAGGGAAA TCTCTGACTA 
     1621 CATACGGACT CAGTGCTTAC AGTGGAAAGC TGAGGTATAT CTGTTCTGCC TTGGGATGTC 
     1681 GCCGATGGGA TAGTGATGAA ATGGAAGAAG AGGAAGACAT CTTGCTTCTG CAGCGTACGC 
     1741 AGAAGACTGT GCGAGCTGTC GGGCCTCGAA GCGGCAGTGA GAAGTGGAAT TTCAGTGTTG 
     1801 GCCACTTTGA ACTTCGGTAT ATTCCAGACA TGGAAACTAG AGCCGGATTC ATTGAAAGCA 
     1861 CCTTTAAACC GGGTGGAAAC AAAGAAGACT CTAAAATTAT TTCAGATGTG GAAGAACAAG 
     1921 AAGCCACCAT GCTGGACACA GTGATAAAAG TTTCCGTTGC TGATTGGAAG GTCATGGCGT 
     1981 TTAGTAGGAA GGGAGGCCGC CTGGAATGGG AGTACCAGTT TTGTACTCCC ATCGCGTCCG 
     2041 CCTGGCTGGT GAGGGATGGC AAGGTCATCC CCATCAGCCT GTTTGATGAT ACAAGTTACA 
     2101 CAGCCAGCGA AGAAGCCTTG GGAGACGAAG AAGACATTGT AGAGGCTGCT CGGGGAGCCA 
     2161 CAGAGAACAG CGTGTACTTA GGGATGTACA GAGGCCAGCT GTACCTGCAG TCGTCCGTCA 
     2221 GGGTCTCAGA AAAGTTCCCT ACAAGCCCAA AGGCCTTGGA GTCTGTAAAT GGCGAAAATG 
     2281 CAATTATTCC TCTGCCGACG ATCAAATGGA AGCCCTTAAT CCATTCTCCT TCTAGGACTC 
     2341 CTGTCTTGGT TGGGTCTGAT GAATTTGACA AATGTCTAAG TAATGATAAG TATTCCCACG 
     2401 AAGAATACAG TAATGGTGCA CTTTCAATCC TCCAGTATCC ATACGATAAC GGTTACTATC 
     2461 TGCCATACTA CAAGAGAGAA AGGAATAAGC GGAGCACGCA GATCACAGTC AGGTTCCTGG 
     2521 ACAGCCCCCA CTACAGCAAG AACATCCGCA AGAAGGACCC TATCCTCCTG CTGCACTGGT 
     2581 GGAAGGAGAT ATTCGGGACG ATCCTGCTTT GCATCGTAGC CACGACCTTC ATCGTGCGCA 
     2641 GGCTTTTCCA TCCTCAGCCC CACAGGCAGC GGAAGGAGTC TGAAACTCAG TGCCAGACTG 
     2701 AAAGTAAATA CGACTCCGTG AGTGCCGATG TCAGTGACAA CAGCTGGAAT GACATGAAGT 
     2761 ACTCAGGATA CGTATCCCGA TATCTAACAG ATTTTGAGCC AATTCAGTGC ATGGGTCGTG 
     2821 GTGGCTTTGG CGTTGTCTTT GAAGCTAAAA ACAAAGTAGA TGACTGCAAT TACGCTATCA 
     2881 AGAGGATCCG GCTCCCCAAC AGGGAGTTGG CACGGGAGAA GGTAATGCGG GAAGTTAAAG 
     2941 CCTTGGCTAA GCTGGAACAC CCAGGCATTG TGAGGTATTT CAACGCCTGG CTGGAAACCC 
     3001 CACCAGAGAA GTGGCAAGAA GAGATGGATG AGATCTGGCT CAAAGACGAA AGCACAGACT 
     3061 GGCCGCTCAG CTCCCCTAGC CCGATGGATG CCCCATCTGT TAAGATCCGA AGGATGGATC 
     3121 CTTTCTCTAC AAAAGAGCAG ATCGAAGTCA TAGCTCCTTC TCCTGAAAGA AGTCGGTCTT 
     3181 TCTCGGTGGG CATTTCCTGT GGCCAGACAA GCTCATCGGA GAGCCAGTTC TCTCCCCTGG 
     3241 AGTTCTCAGG GACAGACTGC GGAGACAACA GTGACTCAGC GGACGCAGCC TACAACCTCC 
     3301 AGGACAGTTG CCTGACGGAC TGCGAGGACG TGGAAGATGG CACCGTGGAC GGCAATGACG 
     3361 AGGGACACTC CTTTGAACTT TGTCCGTCCG AAGCTTCTCC CTATACCCGG TCAAGGGAAG 
     3421 GAACGTCCTC CTCCATAGTG TTTGAGGACT CTGGCTGCGG CAACGCGTCC AGTAAGGAGG 
     3481 AGCCCAGAGG GAACCGGCTG CATGATGGCA ACCATTATGT TAATAAGCTA ACTGATCTCA 
     3541 AGTGCTCCAG CAGCAGGTCT TCTTCAGAAG CCACCACCTT GTCTACCTCC CCTACCAGGC 
     3601 CAACCACTCT AAGCTTGGAT TTCACCAAGA ACACTGTGGG CCAGCTCCAG CCCAGCTCCC 
     3661 CCAAGGTGTA TCTGTACATT CAGATGCAGC TGTGCAGGAA GGAGAACCTC AAAGACTGGA 
     3721 TGAACCGGCG CTGCAGCTTG GAGGACCGGG AGCACGGCGT GTGCCTGCAC ATCTTCCTGC 
     3781 AGATCGCAGA GGCAGTGGAG TTCCTGCACA GCAAGGGACT CATGCACAGG GACCTCAAGC 
     3841 CTTCCAACAT ATTCTTCACA ATGGATGATG TGGTCAAGGT TGGGGACTTT GGACTGGTGA 
     3901 CTGCTATGGA CCAAGATGAA GAAGAGCAGA CTGTACTGAC TCCAATGCCA GCCTATGCTA 
     3961 CGCACACGGG ACAAGTAGGG ACCAAGCTAT ACATGAGCCC AGAGCAGATT CATGGAAACA 
     4021 ACTACTCCCA TAAAGTGGAC ATCTTCTCTT TAGGCTTGAT TCTGTTTGAA CTCCTCTACC 
     4081 CATTCAGCAC CCTGATGGAA CGAGTCCGGA TTTTAACTGA TGTCAGAAAT CTCAAGTTTC 
     4141 CTCTACTGTT CACTCAGAAA TATCCCCAAG AGCATATGAT GGTTCAAGAC ATGCTCTCTC 
     4201 CATCCCCCAC GGAGCGGCCT GAAGCCACAG ACATCATTGA AAATGCCATA TTTGAGAACT 
     4261 TGGAGTTTCC CGGGAAAACG GTTCTGAGAC AGCGGTCCCG CTCCATGAGT TCATCTGGAA 
     4321 CAAAACATTC CAGACAGCCC AGCTGCTCGT ACAGCCCACT GCCTGGCAAC AGCGGACCGA 
     4381 CGCGTACGCG GCCGCTCGAG CAGAAACTCA TCTCAGAAGA GGATCTGGCA GCAAATGATA 
     4441 TCCTGGATTA CAAGGATGAC GACGATAAGG TTTAAACGGC CGGCCGCGGT CATAGCTGTT 
     4501 TCCTGAACAG ATCCCGGGTG GCATCCCTGT GACCCCTCCC CAGTGCCTCT CCTGGCCCTG 
     4561 GAAGTTGCCA CTCCAGTGCC CACCAGCCTT GTCCTAATAA AATTAAGTTG CATCATTTTG 
     4621 TCTGACTAGG TGTCCTTCTA TAATATTATG GGGTGGAGGG GGGTGGTATG GAGCAAGGGG 
     4681 CAAGTTGGGA AGACAACCTG TAGGGCCTGC GGGGTCTATT GGGAACCAAG CTGGAGTGCA 
     4741 GTGGCACAAT CTTGGCTCAC TGCAATCTCC GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT 
     4801 CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA TGCATGACCA GGCTCAGCTA ATTTTTGTTT 
     4861 TTTTGGTAGA GACGGGGTTT CACCATATTG GCCAGGCTGG TCTCCAACTC CTAATCTCAG 
     4921 GTGATCTACC CACCTTGGCC TCCCAAATTG CTGGGATTAC AGGCGTGAAC CACTGCTCCC 
     4981 TTCCCTGTCC TTCTGATTTT AAAATAACTA TACCAGCAGG AGGACGTCCA GACACAGCAT 
     5041 AGGCTACCTG GCCATGCCCA ACCGGTGGGA CATTTGAGTT GCTTGCTTGG CACTGTCCTC 
     5101 TCATGCGTTG GGTCCACTCA GTAGATGCCT GTTGAATTGG GTACGCGGCC AGCGGCGAGC 
     5161 GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG 
     5221 AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT 
     5281 GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA 
     5341 GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT 
     5401 CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC 
     5461 GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT 
     5521 TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC 
     5581 CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC 
     5641 CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG 
     5701 GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC 
     5761 AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG 
     5821 CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA 
     5881 TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT 
     5941 TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG 
     6001 TTTTAAATCA ATCTAAAGTA TATATGAGTA ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC 
     6061 GACGTTGGCT GCGAGCCCTG GGCCTTCACC CGAACTTGGG GGGTGGGGTG GGGAAAAGGA 
     6121 AGAAACGCGG GCGTATTGGC CCCAATGGGG TCTCGGTGGG GTATCGACAG AGTGCCAGCC 
     6181 CTGGGACCGA ACCCCGCGTT TATGAACAAA CGACCCAACA CCGTGCGTTT TATTCTGTCT 
     6241 TTTTATTGCC GTCATAGCGC GGGTTCCTTC CGGTATTGTC TCCTTCCGTG TTTCAGTTAG 
     6301 CCTCCCCCTA GGGTGGGCGA AGAACTCCAG CATGAGATCC CCGCGCTGGA GGATCATCCA 
     6361 GCCGGCGTCC CGGAAAACGA TTCCGAAGCC CAACCTTTCA TAGAAGGCGG CGGTGGAATC 
     6421 GAAATCTCGT GATGGCAGGT TGGGCGTCGC TTGGTCGGTC ATTTCGAACC CCAGAGTCCC 
     6481 GCTCAGAAGA ACTCGTCAAG AAGGCGATAG AAGGCGATGC GCTGCGAATC GGGAGCGGCG 
     6541 ATACCGTAAA GCACGAGGAA GCGGTCAGCC CATTCGCCGC CAAGCTCTTC AGCAATATCA 
     6601 CGGGTAGCCA ACGCTATGTC CTGATAGCGA TCCGCCACAC CCAGCCGGCC ACAGTCGATG 
     6661 AATCCAGAAA AGCGGCCATT TTCCACCATG ATATTCGGCA AGCAGGCATC GCCATGGGTC 
     6721 ACGACGAGAT CCTCGCCGTC GGGCATGCTC GCCTTGAGCC TGGCGAACAG TTCGGCTGGC 
     6781 GCGAGCCCCT GATGCTCTTC GTCCAGATCA TCCTGATCGA CAAGACCGGC TTCCATCCGA 
     6841 GTACGTGCTC GCTCGATGCG ATGTTTCGCT TGGTGGTCGA ATGGGCAGGT AGCCGGATCA 
     6901 AGCGTATGCA GCCGCCGCAT TGCATCAGCC ATGATGGATA CTTTCTCGGC AGGAGCAAGG 
     6961 TGAGATGACA GGAGATCCTG CCCCGGCACT TCGCCCAATA GCAGCCAGTC CCTTCCCGCT 
     7021 TCAGTGACAA CGTCGAGCAC AGCTGCGCAA GGAACGCCCG TCGTGGCCAG CCACGATAGC 
     7081 CGCGCTGCCT CGTCTTGCAG TTCATTCAGG GCACCGGACA GGTCGGTCTT GACAAAAAGA 
     7141 ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC ACGGCGGCAT CAGAGCAGCC GATTGTCTGT 
     7201 TGTGCCCAGT CATAGCCGAA TAGCCTCTCC ACCCAAGCGG CCGGAGAACC TGCGTGCAAT 
     7261 CCATCTTGTT CAATCATGCG AAACGATCCT CATCCTGTCT CTTGATCGAT CTTTGCAAAA 
     7321 GCCTAGGCCT CCAAAAAAGC CTCCTCACTA CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC 
     7381 CTCGGCCTCT GCATAAATAA AAAAAATTAG TCAGCCATGG GGCGGAGAAT GGGCGGAACT 
     7441 GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT TAGGGGCGGG ACTATGGTTG CTGACTAATT 
     7501 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCTGGT 
     7561 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     7621 TCCACACCCT AACTGACACA CATTCCACAG CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT 
     7681 TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA 
     7741 TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT 
     7801 GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC 
     7861 GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC 
     7921 ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT 
     7981 AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG 
     8041 CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT 
     8101 GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA 
     8161 TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT 
     8221 AACGCGAATT TT
//