LOCUS       MR211653                8226 bp    DNA     circular  SYN 
DEFINITION  Slc12a5 (Myc-DDK-tagged) - Mouse solute carrier family 12, member 5 (Slc12a5)
ACCESSION   NM_020333
VERSION     NM_020333.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4383..4474 
                     /label="Myc-DDK"  
     misc_feature    4504..5089 
                     /label="PolyA signal"  
     misc_feature    4561..4580 
                     /label="Restriction Enzyme"  
     misc_feature    5365..6037 
                     /label="ColE1"  
     misc_feature    6467..7261 
                     /label="Kan/Neo"  
     misc_feature    7296..7598 
                     /label="SV40 ori"  
     misc_feature    7684..8161 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4365..4371 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4365 
                     /label="Slc12a5"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCTCAACAAC CTGACGGACT GCGAGGACGG CGATGGGGGA GCCAACCCCG 
     1081 GTGATGGCAA CCCCAAAGAG AGCAGTCCCT TCATCAACAG CACGGACACG GAGAAGGGCA 
     1141 GAGAGTACGA TGGCAGGAAC ATGGCCCTGT TTGAGGAGGA GATGGACACC AGCCCCATGG 
     1201 TATCCTCCCT GCTCAGTGGG CTGGCCAACT ACACCAACCT ACCCCAGGGA AGTAGAGAGC 
     1261 ATGAAGAAGC AGAAAATAAT GAGGGTGGAA AAAAGAAGCC GGTGCAGGCT CCTCGAATGG 
     1321 GCACCTTCAT GGGTGTGTAC CTGCCGTGCC TGCAGAACAT CTTTGGTGTC ATCCTCTTCC 
     1381 TGCGGCTCAC GTGGGTGGTG GGCATCGCGG GCATCATGGA GTCCTTCTGT ATGGTCTTCA 
     1441 TTTGCTGCTC CTGTACGATG CTCACAGCCA TTTCCATGAG TGCAATCGCA ACCAATGGTG 
     1501 TTGTGCCTGC TGGTGGCTCG TACTACATGA TTTCCAGGTC TCTGGGCCCG GAGTTTGGGG 
     1561 GCGCCGTGGG CCTCTGCTTC TACCTGGGCA CCACCTTTGC TGGGGCTATG TACATCCTTG 
     1621 GCACGATCGA GATCCTGCTG GCTTATCTCT TCCCAGCTAT GGCCATCTTC AAGGCAGAAG 
     1681 ATGCCAGTGG GGAGGCGGCC GCCATGCTGA ACAACATGCG GGTGTATGGC ACCTGTGTGC 
     1741 TCACCTGCAT GGCCACCGTT GTCTTTGTGG GTGTCAAGTA CGTCAACAAG TTTGCCTTGG 
     1801 TCTTCCTGGG TTGCGTCATC CTGTCCATCC TGGCCATCTA TGCAGGGGTC ATCAAGTCTG 
     1861 CCTTCGACCC ACCCAATTTC CCGATCTGCC TCCTGGGGAA CCGCACGCTG TCTCGCCATG 
     1921 GCTTTGATGT CTGTGCCAAG CTGGCTTGGG AAGGAAATGA GACAGTGACC ACACGGCTCT 
     1981 GGGGCCTTTT CTGCTCCTCC CGCCTCCTCA ATGCCACCTG TGATGAGTAC TTCACCCGAA 
     2041 ACAATGTCAC AGAGATCCAG GGCATTCCTG GTGCTGCCAG TGGTCTCATC AAAGAGAACC 
     2101 TGTGGAGTTC TTACCTGACC AAAGGGGTGA TTGTCGAGAG GCGTGGGATG CCCTCTGTGG 
     2161 GCCTGGCAGA CGGTACCCCC GTAGACATGG ACCACCCCTA TGTCTTCAGT GATATGACCT 
     2221 CCTACTTCAC CCTGCTCGTT GGTATCTACT TCCCCTCAGT CACAGGGATC ATGGCTGGCT 
     2281 CAAACCGATC TGGAGACCTG CGGGATGCCC AGAAGTCTAT CCCTACTGGA ACTATCCTGG 
     2341 CCATTGCTAC CACCTCTGCT GTCTACATCA GCTCTGTTGT TCTGTTTGGA GCCTGCATCG 
     2401 AGGGGGTCGT CTTACGGGAC AAGTTTGGGG AAGCTGTGAA TGGCAACTTG GTGGTGGGCA 
     2461 CCCTGGCCTG GCCTTCTCCC TGGGTCATCG TCATAGGCTC TTTCTTCTCT ACCTGTGGGG 
     2521 CTGGATTACA GAGCCTCACA GGGGCCCCAC GTCTGCTGCA GGCCATCTCC CGGGATGGCA 
     2581 TAGTGCCCTT CCTGCAGGTC TTTGGCCATG GCAAAGCTAA TGGAGAGCCA ACCTGGGCGC 
     2641 TGCTGCTGAC TGCCTGCATC TGTGAGATCG GCATCCTCAT AGCCTCCCTG GATGAGGTCG 
     2701 CCCCTATACT TTCCATGTTC TTCCTAATGT GTTACATGTT TGTGAACTTG GCTTGTGCGG 
     2761 TGCAGACGCT GCTGAGGACA CCCAACTGGA GGCCACGATT TCGCTATTAC CACTGGACTC 
     2821 TCTCCTTCCT GGGCATGAGC CTCTGCCTGG CCCTCATGTT CATTTGCTCC TGGTACTACG 
     2881 CACTGGTGGC CATGCTCATT GCCGGACTCA TTTATAAGTA CATCGAGTAC CGGGGGGCGG 
     2941 AGAAGGAGTG GGGGGATGGA ATCCGAGGCC TGTCTCTCAG TGCAGCACGC TATGCTCTCT 
     3001 TGCGCCTGGA GGAAGGACCT CCGCATACGA AGAACTGGAG GCCCCAGCTG CTGGTGCTGG 
     3061 TGCGTGTGGA CCAGGATCAG AACGTGGTGC ATCCGCAGCT GCTCTCCCTG ACCTCCCAGC 
     3121 TCAAGGCAGG GAAGGGCCTG ACCATTGTGG GCTCCGTCCT TGAGGGCACC TTTCTGGACA 
     3181 ACCATCCACA GGCTCAGCGG GCAGAGGAGT CTATCAGGCG CCTGATGGAG GCTGAGAAGG 
     3241 TGAAGGGCTT CTGCCAGGTA GTGATCTCCT CCAACCTGCG TGATGGTGTG TCCCACCTGA 
     3301 TCCAGTCTGG GGGCCTCGGG GGATTGCAAC ACAATACCGT GCTGGTGGGC TGGCCTCGCA 
     3361 ACTGGAGGCA GAAGGAGGAT CATCAGACAT GGAGGAACTT CATCGAACTG GTCCGGGAAA 
     3421 CTACAGCCGG CCACCTCGCC CTGCTGGTCA CCAAGAATGT TTCCATGTTT CCCGGGAACC 
     3481 CTGAGCGCTT CTCGGAGGGC AGCATTGACG TGTGGTGGAT TGTGCACGAC GGGGGCATGC 
     3541 TCATGCTGCT GCCCTTCCTG CTGCGACACC ACAAGGTCTG GAGGAAATGC AAAATGCGGA 
     3601 TCTTCACCGT GGCCCAGATG GACGATAACA GTATCCAGAT GAAGAAGGAC CTGACCACGT 
     3661 TTCTGTACCA CTTACGCATT ACTGCAGAGG TGGAGGTGGT GGAGATGCAT GAGAGCGACA 
     3721 TCTCGGCATA CACCTACGAG AAGACATTAG TAATGGAGCA ACGATCTCAG ATCCTCAAAC 
     3781 AGATGCACCT CACCAAGAAC GAGCGGGAAC GGGAGATCCA GAGCATCACA GACGAGTCTC 
     3841 GGGGCTCCAT TCGGAGGAAG AATCCAGCCA ACCCCCGGCT CCGCCTCAAT GTTCCCGAAG 
     3901 AGACAGCGTG TGACAATGAG GAGAAGCCAG AGGAGGAGGT GCAGCTGATC CATGACCAGA 
     3961 GTGCTCCCAG CTGCCCTAGC AGCTCGCCAT CTCCAGGGGA GGAGCCCGAG GGGGAGAGGG 
     4021 AGACAGACCC AGAGGTGCAT CTTACCTGGA CCAAGGATAA GTCAGTGGCA GAGAAGAATA 
     4081 AAGGCCCCAG TCCCGTCTCC TCCGAGGGCA TCAAGGACTT CTTCAGCATG AAGCCGGAGT 
     4141 GGGAAAACTT GAACCAGTCC AATGTACGGC GCATGCACAC AGCTGTGCGG CTGAACGAGG 
     4201 TCATCGTGAA TAAATCTCGG GATGCCAAGC TAGTTTTGCT CAACATGCCC GGGCCTCCCC 
     4261 GCAACCGCAA TGGGGATGAA AACTACATGG AATTCTTGGA GGTCCTCACT GAGCAACTGG 
     4321 ACCGGGTGAT GCTGGTCCGC GGTGGCGGCC GAGAGGTCAT CACCATCTAC TCCACGCGTA 
     4381 CGCGGCCGCT CGAGCAGAAA CTCATCTCAG AAGAGGATCT GGCAGCAAAT GATATCCTGG 
     4441 ATTACAAGGA TGACGACGAT AAGGTTTAAA CGGCCGGCCG CGGTCATAGC TGTTTCCTGA 
     4501 ACAGATCCCG GGTGGCATCC CTGTGACCCC TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT 
     4561 GCCACTCCAG TGCCCACCAG CCTTGTCCTA ATAAAATTAA GTTGCATCAT TTTGTCTGAC 
     4621 TAGGTGTCCT TCTATAATAT TATGGGGTGG AGGGGGGTGG TATGGAGCAA GGGGCAAGTT 
     4681 GGGAAGACAA CCTGTAGGGC CTGCGGGGTC TATTGGGAAC CAAGCTGGAG TGCAGTGGCA 
     4741 CAATCTTGGC TCACTGCAAT CTCCGCCTCC TGGGTTCAAG CGATTCTCCT GCCTCAGCCT 
     4801 CCCGAGTTGT TGGGATTCCA GGCATGCATG ACCAGGCTCA GCTAATTTTT GTTTTTTTGG 
     4861 TAGAGACGGG GTTTCACCAT ATTGGCCAGG CTGGTCTCCA ACTCCTAATC TCAGGTGATC 
     4921 TACCCACCTT GGCCTCCCAA ATTGCTGGGA TTACAGGCGT GAACCACTGC TCCCTTCCCT 
     4981 GTCCTTCTGA TTTTAAAATA ACTATACCAG CAGGAGGACG TCCAGACACA GCATAGGCTA 
     5041 CCTGGCCATG CCCAACCGGT GGGACATTTG AGTTGCTTGC TTGGCACTGT CCTCTCATGC 
     5101 GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA TTGGGTACGC GGCCAGCGGC GAGCGGTATC 
     5161 AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA 
     5221 CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT 
     5281 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG 
     5341 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG 
     5401 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG 
     5461 CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC 
     5521 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA 
     5581 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG 
     5641 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC 
     5701 TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC 
     5761 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG 
     5821 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT 
     5881 GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT 
     5941 CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA 
     6001 ATCAATCTAA AGTATATATG AGTAACCTGA GGCTATGGCA GGGCCTGCCG CCCCGACGTT 
     6061 GGCTGCGAGC CCTGGGCCTT CACCCGAACT TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC 
     6121 GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG TGGGGTATCG ACAGAGTGCC AGCCCTGGGA 
     6181 CCGAACCCCG CGTTTATGAA CAAACGACCC AACACCGTGC GTTTTATTCT GTCTTTTTAT 
     6241 TGCCGTCATA GCGCGGGTTC CTTCCGGTAT TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC 
     6301 CCTAGGGTGG GCGAAGAACT CCAGCATGAG ATCCCCGCGC TGGAGGATCA TCCAGCCGGC 
     6361 GTCCCGGAAA ACGATTCCGA AGCCCAACCT TTCATAGAAG GCGGCGGTGG AATCGAAATC 
     6421 TCGTGATGGC AGGTTGGGCG TCGCTTGGTC GGTCATTTCG AACCCCAGAG TCCCGCTCAG 
     6481 AAGAACTCGT CAAGAAGGCG ATAGAAGGCG ATGCGCTGCG AATCGGGAGC GGCGATACCG 
     6541 TAAAGCACGA GGAAGCGGTC AGCCCATTCG CCGCCAAGCT CTTCAGCAAT ATCACGGGTA 
     6601 GCCAACGCTA TGTCCTGATA GCGATCCGCC ACACCCAGCC GGCCACAGTC GATGAATCCA 
     6661 GAAAAGCGGC CATTTTCCAC CATGATATTC GGCAAGCAGG CATCGCCATG GGTCACGACG 
     6721 AGATCCTCGC CGTCGGGCAT GCTCGCCTTG AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC 
     6781 CCCTGATGCT CTTCGTCCAG ATCATCCTGA TCGACAAGAC CGGCTTCCAT CCGAGTACGT 
     6841 GCTCGCTCGA TGCGATGTTT CGCTTGGTGG TCGAATGGGC AGGTAGCCGG ATCAAGCGTA 
     6901 TGCAGCCGCC GCATTGCATC AGCCATGATG GATACTTTCT CGGCAGGAGC AAGGTGAGAT 
     6961 GACAGGAGAT CCTGCCCCGG CACTTCGCCC AATAGCAGCC AGTCCCTTCC CGCTTCAGTG 
     7021 ACAACGTCGA GCACAGCTGC GCAAGGAACG CCCGTCGTGG CCAGCCACGA TAGCCGCGCT 
     7081 GCCTCGTCTT GCAGTTCATT CAGGGCACCG GACAGGTCGG TCTTGACAAA AAGAACCGGG 
     7141 CGCCCCTGCG CTGACAGCCG GAACACGGCG GCATCAGAGC AGCCGATTGT CTGTTGTGCC 
     7201 CAGTCATAGC CGAATAGCCT CTCCACCCAA GCGGCCGGAG AACCTGCGTG CAATCCATCT 
     7261 TGTTCAATCA TGCGAAACGA TCCTCATCCT GTCTCTTGAT CGATCTTTGC AAAAGCCTAG 
     7321 GCCTCCAAAA AAGCCTCCTC ACTACTTCTG GAATAGCTCA GAGGCCGAGG CGGCCTCGGC 
     7381 CTCTGCATAA ATAAAAAAAA TTAGTCAGCC ATGGGGCGGA GAATGGGCGG AACTGGGCGG 
     7441 AGTTAGGGGC GGGATGGGCG GAGTTAGGGG CGGGACTATG GTTGCTGACT AATTGAGATG 
     7501 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC TGGTTGCTGA 
     7561 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     7621 CCCTAACTGA CACACATTCC ACAGCTGGTT CTTTCCGCCT CAGGACTCTT CCTTTTTCAA 
     7681 TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT 
     7741 TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGCG 
     7801 CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA 
     7861 CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC 
     7921 GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT 
     7981 TTACGGCACC TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG 
     8041 CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC 
     8101 TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG 
     8161 ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG 
     8221 AATTTT
//