LOCUS       MR211651                8220 bp    DNA     circular  SYN 
DEFINITION  Upf1 (Myc-DDK-tagged) - Mouse UPF1 regulator of nonsense transcripts homolog (yeast) (Upf1), transcript variant 2
ACCESSION   NM_030680
VERSION     NM_030680.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4377..4468 
                     /label="Myc-DDK"  
     misc_feature    4498..5083 
                     /label="PolyA signal"  
     misc_feature    4555..4574 
                     /label="Restriction Enzyme"  
     misc_feature    5359..6031 
                     /label="ColE1"  
     misc_feature    6461..7255 
                     /label="Kan/Neo"  
     misc_feature    7290..7592 
                     /label="SV40 ori"  
     misc_feature    7678..8155 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4359..4365 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4359 
                     /label="Upf1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAGTGTGGAG GCGTACGGCC CCAGCTCGCA AACACTCACC TTCTTGGACA 
     1081 CTGAGGAGGC CGAGCTGCTC GGCGCCGACA CCCAGGGCTC CGAGTTCGAA TTCACCGACT 
     1141 TCACCCTTCC CAGCCAGACG CAGACGCCCC CCGGCGGCCC CGGCGGCGCG GGAGGCCCGG 
     1201 GCGGAGCGGG CGCAGGCGGC GCGGCCGGCC AGCTCGACGC ACAAGTTGGA CCAGAGGGCA 
     1261 TCTTGCAAAA TGGGGCTGTG GATGACAGTG TGGCCAAGAC CAGCCAGCTG CTAGCTGAGC 
     1321 TGAACTTCGA GGAAGATGAA GAGGACACAT ACTACACTAA GGACCTCCCA GTCCACGCCT 
     1381 GCAGTTACTG TGGAATCCAT GATCCTGCCT GCGTGGTTTA CTGTAATACC AGCAAGAAGT 
     1441 GGTTCTGCAA TGGCCGAGGA AATACTTCTG GCAGCCACAT TGTGAATCAC CTCGTGAGGG 
     1501 CAAAATGCAA GGAAGTGACG CTGCACAAGG ACGGGCCTCT GGGCGAGACC GTGCTGGAGT 
     1561 GCTACAACTG TGGCTGCCGC AACGTCTTCC TGCTGGGCTT CATCCCTGCG AAGGCCGACT 
     1621 CTGTGGTGGT GCTGTTGTGC AGGCAGCCCT GTGCCAGCCA GAGCAGCCTG AAGGACATCA 
     1681 ACTGGGACAG CTCACAGTGG CAGCCCCTAA TCCAGGACCG GTGCTTTCTG TCATGGCTGG 
     1741 TCAAGATTCC CTCTGAGCAG GAGCAGCTGC GAGCACGGCA GATCACGGCA CAGCAGATCA 
     1801 ACAAGCTGGA AGAGCTCTGG AAGGAAAATC CTTCAGCCAC TCTGGAGGAC CTGGAGAAGC 
     1861 CAGGCGTAGA CGAGGAGCCA CAGCACGTGC TCCTGCGTTA CGAGGATGCT TACCAGTACC 
     1921 AGAACATCTT CGGGCCACTG GTCAAGCTGG AGGCTGACTA TGACAAGAAG TTGAAGGAGT 
     1981 CACAGACTCA AGATAACATC ACGGTCAGGT GGGACCTGGG CCTTAACAAG AAGAGAATCG 
     2041 CCTTCTTCAC TTTGCCCAAG ACTGACTCTG ACATGCGGCT CATGCAGGGT GATGAGATCT 
     2101 GTCTGCGGTA CAAAGGGGAT CTGGCGCCCC TGTGGAAGGG GATTGGCCAC GTCATCAAGG 
     2161 TTCCTGATAA TTATGGTGAT GAGATTGCTA TTGAGCTCCG CAGCAGCGTG GGTGCCCCTG 
     2221 TGGAAGTGAC CCACAACTTC CAAGTGGATT TTGTGTGGAA GTCAACCTCT TTTGATAGGA 
     2281 TGCAGAGTGC ACTGAAGACC TTCGCTGTGG ACGAGACCTC TGTGTCAGGG TATATTTACC 
     2341 ACAAGCTGCT GGGCCACGAG GTGGAGGATG TGGTCATCAA GTGCCAGCTG CCAAAGCGCT 
     2401 TCACAGCTCA GGGGCTCCCT GACCTCAACC ACTCTCAGGT GTATGCTGTG AAGACCGTGC 
     2461 TGCAGAGACC ACTCAGCCTC ATCCAGGGCC CTCCAGGCAC AGGCAAGACT GTGACATCAG 
     2521 CCACTATTGT CTACCACCTT GCTCGGCAGG GCAATGGGCC TGTACTGGTT TGTGCTCCAA 
     2581 GTAACATCGC TGTGGACCAG CTCACAGAGA AGATCCACCA GACAGGACTG AAGGTCGTAC 
     2641 GCCTCTGTGC CAAGAGCCGT GAGGCCATTG ACTCCCCAGT GTCCTTCCTG GCTTTGCACA 
     2701 ACCAGATCAG GAACATGGAC AGCATGCCTG AGCTGCAGAA GCTGCAGCAG CTAAAGGATG 
     2761 AGACAGGCGA GCTGTCATCT GCAGATGAGA AGCGGTACCG GGCGCTTAAG CGCACAGCTG 
     2821 AGAGAGAACT TCTCATGAAT GCAGATGTCA TATGCTGCAC ATGTGTGGGT GCTGGTGACC 
     2881 CGAGGCTGGC CAAGATGCAG TTCCGTTCCA TCCTCATTGA CGAGAGCACC CAGGCCACTG 
     2941 AGCCTGAGTG CATGGTGCCT GTAGTCCTTG GGGCCAAGCA GCTAATCCTC GTCGGTGACC 
     3001 ACTGCCAGCT GGGCCCAGTG GTGATGTGCA AGAAGGCAGC CAAGGCCGGA CTGTCACAAT 
     3061 CGCTCTTCGA GCGCTTGGTG GTGCTGGGCA TCCGGCCCAT CCGCCTGCAG GTGCAATACC 
     3121 GCATGCACCC TGCACTCAGC GCCTTTCCGT CCAACATCTT CTACGAGGGC TCATTGCAGA 
     3181 ATGGCGTCAC TGCAGCGGAT CGTGTCAAAA AAGGCTTTGA CTTCCAGTGG CCACAACCTG 
     3241 ACAAGCCTAT GTTCTTCTAC GTGACGCAGG GCCAGGAGGA GATTGCCAGC TCTGGCACAT 
     3301 CCTACCTCAA CAGGACGGAG GCAGCCAATG TGGAGAAGAT AACTACGAAG CTGTTGAAGG 
     3361 CAGGTGCAAA GCCTGACCAG ATCGGCATCA TCACCCCCTA CGAGGGCCAG CGCTCTTACT 
     3421 TGGTGCAGTA CATGCAGTTC AGCGGCTCCC TGCACACAAA GCTCTACCAG GAAGTGGAAA 
     3481 TTGCCAGTGT GGACGCCTTC CAGGGCCGGG AGAAGGACTT CATCATTCTG TCCTGCGTGC 
     3541 GCGCCAATGA ACATCAGGGC ATTGGGTTCC TAAACGACCC CCGGCGTCTG AATGTGGCTC 
     3601 TCACCAGAGC AAGATATGGC GTGATCATTG TGGGTAACCC AAAGGCCCTG TCGAAGCAGC 
     3661 CCCTGTGGAA TCACCTGCTG AGCTACTACA AGGAACAGAA GGCGCTAGTG GAAGGGCCGC 
     3721 TCAACAACCT ACGTGAGAGC CTCATGCAGT TCAGCAAGCC TCGCAAACTT GTCAACACTG 
     3781 TCAACCCGGG TGCCCGCTTC ATGACTACTG CCATGTACGA TGCCCGTGAG GCCATCATCC 
     3841 CCGGGTCTGT CTATGACCGC AGCAGCCAGG GCCGGCCCTC GAACATGTAC TTCCAGACCC 
     3901 ATGACCAGAT TAGTATGATC AGCGCAGGCC CCAGCCACGT GGCTGCCATG AACATCCCTA 
     3961 TTCCCTTCAA CTTGGTCATG CCTCCCATGC CGCCACCTGG CTACTTCGGA CAGGCCAACG 
     4021 GGCCGGCAGC TGGTCGGGGC ACCCCAAAAA CCAAGACTGG CCGTGGGGGC CGCCAGAAGA 
     4081 ACCGCTTTGG GCTTCCTGGG CCCAGCCAGA CCACCCTTCC CAACAGCCAG GCCAGCCAGG 
     4141 ACGTGGCCTC CCAGCCCTTT TCACAGGGTG CCCTCACACA GGGTTACGTG TCCATGAGCC 
     4201 AGCCCTCTCA GATGAGCCAG CCTGGCCTCT CCCAGCCAGA ACTGTCCCAG GACAGCTACC 
     4261 TCGGTGATGA GTTTAAATCA CAGATTGACG TGGCACTCTC ACAAGACTCC ACATACCAGG 
     4321 GAGAGCGGGC ATACCAGCAC GGCGGGGTCA CCGGGCTGTC CCAGTACACG CGTACGCGGC 
     4381 CGCTCGAGCA GAAACTCATC TCAGAAGAGG ATCTGGCAGC AAATGATATC CTGGATTACA 
     4441 AGGATGACGA CGATAAGGTT TAAACGGCCG GCCGCGGTCA TAGCTGTTTC CTGAACAGAT 
     4501 CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA GTGCCTCTCC TGGCCCTGGA AGTTGCCACT 
     4561 CCAGTGCCCA CCAGCCTTGT CCTAATAAAA TTAAGTTGCA TCATTTTGTC TGACTAGGTG 
     4621 TCCTTCTATA ATATTATGGG GTGGAGGGGG GTGGTATGGA GCAAGGGGCA AGTTGGGAAG 
     4681 ACAACCTGTA GGGCCTGCGG GGTCTATTGG GAACCAAGCT GGAGTGCAGT GGCACAATCT 
     4741 TGGCTCACTG CAATCTCCGC CTCCTGGGTT CAAGCGATTC TCCTGCCTCA GCCTCCCGAG 
     4801 TTGTTGGGAT TCCAGGCATG CATGACCAGG CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA 
     4861 CGGGGTTTCA CCATATTGGC CAGGCTGGTC TCCAACTCCT AATCTCAGGT GATCTACCCA 
     4921 CCTTGGCCTC CCAAATTGCT GGGATTACAG GCGTGAACCA CTGCTCCCTT CCCTGTCCTT 
     4981 CTGATTTTAA AATAACTATA CCAGCAGGAG GACGTCCAGA CACAGCATAG GCTACCTGGC 
     5041 CATGCCCAAC CGGTGGGACA TTTGAGTTGC TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG 
     5101 TCCACTCAGT AGATGCCTGT TGAATTGGGT ACGCGGCCAG CGGCGAGCGG TATCAGCTCA 
     5161 CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG 
     5221 AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA 
     5281 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA 
     5341 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC 
     5401 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC 
     5461 GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT 
     5521 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG 
     5581 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG 
     5641 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA 
     5701 CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG 
     5761 AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT 
     5821 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT 
     5881 TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG 
     5941 ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT 
     6001 CTAAAGTATA TATGAGTAAC CTGAGGCTAT GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC 
     6061 GAGCCCTGGG CCTTCACCCG AACTTGGGGG GTGGGGTGGG GAAAAGGAAG AAACGCGGGC 
     6121 GTATTGGCCC CAATGGGGTC TCGGTGGGGT ATCGACAGAG TGCCAGCCCT GGGACCGAAC 
     6181 CCCGCGTTTA TGAACAAACG ACCCAACACC GTGCGTTTTA TTCTGTCTTT TTATTGCCGT 
     6241 CATAGCGCGG GTTCCTTCCG GTATTGTCTC CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG 
     6301 GTGGGCGAAG AACTCCAGCA TGAGATCCCC GCGCTGGAGG ATCATCCAGC CGGCGTCCCG 
     6361 GAAAACGATT CCGAAGCCCA ACCTTTCATA GAAGGCGGCG GTGGAATCGA AATCTCGTGA 
     6421 TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT TTCGAACCCC AGAGTCCCGC TCAGAAGAAC 
     6481 TCGTCAAGAA GGCGATAGAA GGCGATGCGC TGCGAATCGG GAGCGGCGAT ACCGTAAAGC 
     6541 ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA AGCTCTTCAG CAATATCACG GGTAGCCAAC 
     6601 GCTATGTCCT GATAGCGATC CGCCACACCC AGCCGGCCAC AGTCGATGAA TCCAGAAAAG 
     6661 CGGCCATTTT CCACCATGAT ATTCGGCAAG CAGGCATCGC CATGGGTCAC GACGAGATCC 
     6721 TCGCCGTCGG GCATGCTCGC CTTGAGCCTG GCGAACAGTT CGGCTGGCGC GAGCCCCTGA 
     6781 TGCTCTTCGT CCAGATCATC CTGATCGACA AGACCGGCTT CCATCCGAGT ACGTGCTCGC 
     6841 TCGATGCGAT GTTTCGCTTG GTGGTCGAAT GGGCAGGTAG CCGGATCAAG CGTATGCAGC 
     6901 CGCCGCATTG CATCAGCCAT GATGGATACT TTCTCGGCAG GAGCAAGGTG AGATGACAGG 
     6961 AGATCCTGCC CCGGCACTTC GCCCAATAGC AGCCAGTCCC TTCCCGCTTC AGTGACAACG 
     7021 TCGAGCACAG CTGCGCAAGG AACGCCCGTC GTGGCCAGCC ACGATAGCCG CGCTGCCTCG 
     7081 TCTTGCAGTT CATTCAGGGC ACCGGACAGG TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC 
     7141 TGCGCTGACA GCCGGAACAC GGCGGCATCA GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA 
     7201 TAGCCGAATA GCCTCTCCAC CCAAGCGGCC GGAGAACCTG CGTGCAATCC ATCTTGTTCA 
     7261 ATCATGCGAA ACGATCCTCA TCCTGTCTCT TGATCGATCT TTGCAAAAGC CTAGGCCTCC 
     7321 AAAAAAGCCT CCTCACTACT TCTGGAATAG CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC 
     7381 ATAAATAAAA AAAATTAGTC AGCCATGGGG CGGAGAATGG GCGGAACTGG GCGGAGTTAG 
     7441 GGGCGGGATG GGCGGAGTTA GGGGCGGGAC TATGGTTGCT GACTAATTGA GATGCATGCT 
     7501 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCTGGTTG CTGACTAATT 
     7561 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCCTAA 
     7621 CTGACACACA TTCCACAGCT GGTTCTTTCC GCCTCAGGAC TCTTCCTTTT TCAATATTAT 
     7681 TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA 
     7741 AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA CGCGCCCTGT 
     7801 AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC 
     7861 AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC 
     7921 TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG 
     7981 CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA 
     8041 TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC 
     8101 CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT TTGATTTATA AGGGATTTTG 
     8161 CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//