LOCUS       MR211649                8217 bp    DNA     circular  SYN 
DEFINITION  Magi2 (Myc-DDK-tagged) - Mouse membrane associated guanylate kinase, WW and PDZ domain containing 2 (Magi2), transcript variant 2
ACCESSION   NM_015823
VERSION     NM_015823.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4374..4465 
                     /label="Myc-DDK"  
     misc_feature    4495..5080 
                     /label="PolyA signal"  
     misc_feature    4552..4571 
                     /label="Restriction Enzyme"  
     misc_feature    5356..6028 
                     /label="ColE1"  
     misc_feature    6458..7252 
                     /label="Kan/Neo"  
     misc_feature    7287..7589 
                     /label="SV40 ori"  
     misc_feature    7675..8152 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4356..4362 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4356 
                     /label="Magi2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAATTGGAG AAAAGTGGTG CTCTCCTAGA AAGCGGGACC TATGAAGACA 
     1081 ACTACTACGG TACCCCGAAG CCTCCAGCTG AACCAGCACC ATTATTAAAT GTAACAGACC 
     1141 AGATACTTCC GGGAGCTACT CCAAGTGCTG AGGGGAAGCG GAAAAGAAAT AAGTCAGTGA 
     1201 CCAACATGGA GAAAGCAAGT ATAGAGCCTC CAGAGGAGGA AGAAGAAGAA AGGCCTGTAG 
     1261 TCAATGGAAA CGGCGTGGTC ATAACCCCAG AATCCAGTGA ACATGAAGAC AAAAGTGCAG 
     1321 GTGCCTCAGG GGAGACACCC TCCCAGCCTT ACCCTGCACC CGTGTACAGC CAGCCCGAAG 
     1381 AGCTCAAGGA CCAGATGGAC GATACAAAGC CAACAAAGCC TGAGGAGAAC GAGGACTCTG 
     1441 ATCCATTGCC TGATAACTGG GAAATGGCCT ACACAGAGAA GGGGGAAGTC TACTTCATTG 
     1501 ACCATAACAC AAAGACAACA TCATGGCTGG ATCCGCGACT TGCGAAAAAG GCTAAACCTC 
     1561 CAGAAGAGTG CAAAGAAAAT GAGCTTCCAT ATGGCTGGGA AAAAATCGAT GATCCTATAT 
     1621 ATGGCACTTA CTATGTTGAC CACATAAATA GAAGAACACA GTTTGAAAAC CCTGTCCTGG 
     1681 AAGCAAAAAG GAAGCTACAG CAACATAACA TGCCCCACAC AGAACTTGGA GCAAAGCCCC 
     1741 TGCAGGCCCC AGGTTTCCGA GAAAAGCCAC TCTTCACCCG GGATGCATCC CAGTTGAAGG 
     1801 GAACGTTCCT CAGCACCACC CTCAAAAAGA GCAACATGGG CTTTGGGTTT ACCATCATTG 
     1861 GTGGAGACGA GCCGGATGAG TTTCTACAGG TGAAAAGTGT GATCCCGGAT GGGCCTGCCG 
     1921 CACAGGATGG GAAAATGGAG ACAGGTGATG TCATTGTCTA TATTAATGAA GTTTGTGTCC 
     1981 TTGGACACAC TCATGCAGAT GTTGTCAAAC TTTTCCAGTC TGTTCCTATT GGTCAGAGTG 
     2041 TCAACTTGGT GTTGTGTCGT GGCTACCCTT TGCCCTTTGA CCCTGAAGAT CCTGCTAACA 
     2101 GCATGGTGCC ACCCCTTGCA ATAATGGAGA GGCCACCTCC GGTGATGGTC AATGGAAGAC 
     2161 ATAACTATGA AACATACTTG GAATACATTT CTCGGACCTC ACAGTCGGTC CCAGATATTA 
     2221 CAGACCGGCC ACCTCATTCT TTGCACTCCA TGCCAGCTGA CGGCCAGCTA GATGGCACGT 
     2281 ATCCACCACC CGTCCATGAC GACAATGTGT CTATGGCTTC GTCTGGAGCC ACTCAAGCTG 
     2341 AACTTATGAC CTTAACCATT GTGAAAGGTG CCCAGGGATT TGGCTTTACT ATTGCCGACA 
     2401 GTCCCACGGG ACAGCGGGTG AAACAAATCC TTGACATTCA GGGATGCCCT GGGCTGTGTG 
     2461 AAGGAGACCT CATTGTTGAG ATCAACCAAC AGAATGTACA GAACCTGAGC CATACAGAAG 
     2521 TAGTGGATAT ACTTAAGGAC TGCCCCGTTG GAAGTGAGAC TTCTTTAATC ATCCATCGAG 
     2581 GAGGTTTCTT TTCTCCATGG AAAACTCCAA AGCCTATGAT GGACCGATGG GAGAACCAAG 
     2641 GCAGTCCACA AACAAGTTTA TCTGCTCCGG CCGTCCCACA GAACCTGCCC TTCCCACCTG 
     2701 CCCTTCACAG GAGCTCCTTT CCTGATTCAA CAGAGGCCTT TGACCCACGG AAGCCTGACC 
     2761 CATATGAGCT CTACGAGAAA TCGAGAGCCA TTTATGAAAG TAGGCAACAA GTGCCACCCA 
     2821 GGACCAGTTT TCGAATGGAT TCCTCTGGTC CAGATTATAA GGAACTGGAT GTTCACCTTC 
     2881 GGAGGATGGA GTCTGGATTT GGCTTTAGAA TCCTTGGGGG AGATGAACCT GGACAGCCTA 
     2941 TTTTGATCGG AGCCGTCATT GCCATGGGCT CAGCTGACAG AGACGGCCGT CTACACCCAG 
     3001 GAGATGAGCT TGTCTATGTC GATGGGATCC CAGTGGCTGG CAAGACCCAC CGCTATGTCA 
     3061 TCGACCTCAT GCACCACGCG GCCCGCAATG GGCAGGTTAA CCTCACTGTG AGAAGAAAGG 
     3121 TGCTATGTGG AGGGGAGCCC TGCCCAGAGA ATGGGAGGAG TCCAGGCTCT GTATCAACTC 
     3181 ACCACAGCTC TCCGCGCAGT GACTATGCCA CCTACTCCAA CAGCAACCAC GCCGCCCCCA 
     3241 GCAGCAATGC CTCACCTCCT GAAGGCTTTG CCTCACACAG CTTGCAGACC AGTGATGTGG 
     3301 TCATTCACCG CAAAGAAAAC GAAGGGTTTG GCTTCGTCAT CATCAGCTCT CTGAACAGGC 
     3361 CTGAGTCTGG AGCCACCATA ACTGTGCCCC ATAAAATTGG ACGAATCATT GATGGGAGCC 
     3421 CTGCAGATCG CTGTGCCAAA CTCAAAGTGG GCGACCGTAT CTTAGCAGTC AACGGCCAGT 
     3481 CTATCATCAA CATGCCTCAC GCTGACATTG TGAAGCTCAT CAAGGACGCC GGTCTCAGTG 
     3541 TCACCCTTCG CATCATTCCT CAGGAGGAGC TCAACAGCCC AACATCAGCA CCCAGTTCAG 
     3601 AGAAACAGAG CCCCATGGCC CAGCAGCACA GCCCTCTGGC CCAGCAGAGT CCTCTGGCCC 
     3661 AGCCAAGCCC CGCCACCCCC AACAGCCCAG TCGCACAGCC AGCTCCTCCC CAACCTCTCC 
     3721 AGCTGCAAGG ACACGAAAAT AGTTACAGGT CAGAAGTTAA AGCGAGGCAA GATGTGAAGC 
     3781 CAGACATCCG GCAGCCTCCC TTCACAGACT ACAGGCAGCC CCCGCTGGAC TACAGGCAGC 
     3841 CCCCGGGAGG AGACTACTCA CAGCCCCCAC CCTTGGACTA CAGGCAGCAC TCTCCAGACA 
     3901 CCAGGCAGTA CCCTCTGTCA GACTACAGGC AGCCACAGGA TTTTGATTAT TTCACTGTGG 
     3961 ACATGGAGAA AGGAGCCAAA GGATTTGGAT TCAGCATTCG TGGAGGAAGG GAATACAAGA 
     4021 TGGATCTGTA TGTGTTGAGA TTGGCAGAGG ATGGGCCAGC CATAAGGAAC GGCAGGATGA 
     4081 GGGTAGGAGA TCAGATCATT GAAATAAATG GGGAAAGCAC ACGAGACATG ACCCACGCCA 
     4141 GAGCAATAGA ACTCATCAAG TCTGGAGGAA GAAGAGTGCG GCTGCTGCTG AAGAGAGGCA 
     4201 CGGGGCAGGT CCCGGAGTAT GGAATGGTAC CTTCCAGCCT CTCCATGTGC ATGAAAAGTG 
     4261 ACAAGCATGG GTCCCCATAT TTCTACTTAC TGGGCCACCC TAAAGACACG ACGAACCCCA 
     4321 CGCCTGGAGT GCTGCCGCTG CCGCCGCCCC AGGCCTGCCG GAAGACGCGT ACGCGGCCGC 
     4381 TCGAGCAGAA ACTCATCTCA GAAGAGGATC TGGCAGCAAA TGATATCCTG GATTACAAGG 
     4441 ATGACGACGA TAAGGTTTAA ACGGCCGGCC GCGGTCATAG CTGTTTCCTG AACAGATCCC 
     4501 GGGTGGCATC CCTGTGACCC CTCCCCAGTG CCTCTCCTGG CCCTGGAAGT TGCCACTCCA 
     4561 GTGCCCACCA GCCTTGTCCT AATAAAATTA AGTTGCATCA TTTTGTCTGA CTAGGTGTCC 
     4621 TTCTATAATA TTATGGGGTG GAGGGGGGTG GTATGGAGCA AGGGGCAAGT TGGGAAGACA 
     4681 ACCTGTAGGG CCTGCGGGGT CTATTGGGAA CCAAGCTGGA GTGCAGTGGC ACAATCTTGG 
     4741 CTCACTGCAA TCTCCGCCTC CTGGGTTCAA GCGATTCTCC TGCCTCAGCC TCCCGAGTTG 
     4801 TTGGGATTCC AGGCATGCAT GACCAGGCTC AGCTAATTTT TGTTTTTTTG GTAGAGACGG 
     4861 GGTTTCACCA TATTGGCCAG GCTGGTCTCC AACTCCTAAT CTCAGGTGAT CTACCCACCT 
     4921 TGGCCTCCCA AATTGCTGGG ATTACAGGCG TGAACCACTG CTCCCTTCCC TGTCCTTCTG 
     4981 ATTTTAAAAT AACTATACCA GCAGGAGGAC GTCCAGACAC AGCATAGGCT ACCTGGCCAT 
     5041 GCCCAACCGG TGGGACATTT GAGTTGCTTG CTTGGCACTG TCCTCTCATG CGTTGGGTCC 
     5101 ACTCAGTAGA TGCCTGTTGA ATTGGGTACG CGGCCAGCGG CGAGCGGTAT CAGCTCACTC 
     5161 AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC 
     5221 AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG 
     5281 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC 
     5341 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT 
     5401 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT 
     5461 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG 
     5521 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT 
     5581 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT 
     5641 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG 
     5701 CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA 
     5761 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT 
     5821 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC 
     5881 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT 
     5941 ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA 
     6001 AAGTATATAT GAGTAACCTG AGGCTATGGC AGGGCCTGCC GCCCCGACGT TGGCTGCGAG 
     6061 CCCTGGGCCT TCACCCGAAC TTGGGGGGTG GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA 
     6121 TTGGCCCCAA TGGGGTCTCG GTGGGGTATC GACAGAGTGC CAGCCCTGGG ACCGAACCCC 
     6181 GCGTTTATGA ACAAACGACC CAACACCGTG CGTTTTATTC TGTCTTTTTA TTGCCGTCAT 
     6241 AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG 
     6301 GGCGAAGAAC TCCAGCATGA GATCCCCGCG CTGGAGGATC ATCCAGCCGG CGTCCCGGAA 
     6361 AACGATTCCG AAGCCCAACC TTTCATAGAA GGCGGCGGTG GAATCGAAAT CTCGTGATGG 
     6421 CAGGTTGGGC GTCGCTTGGT CGGTCATTTC GAACCCCAGA GTCCCGCTCA GAAGAACTCG 
     6481 TCAAGAAGGC GATAGAAGGC GATGCGCTGC GAATCGGGAG CGGCGATACC GTAAAGCACG 
     6541 AGGAAGCGGT CAGCCCATTC GCCGCCAAGC TCTTCAGCAA TATCACGGGT AGCCAACGCT 
     6601 ATGTCCTGAT AGCGATCCGC CACACCCAGC CGGCCACAGT CGATGAATCC AGAAAAGCGG 
     6661 CCATTTTCCA CCATGATATT CGGCAAGCAG GCATCGCCAT GGGTCACGAC GAGATCCTCG 
     6721 CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG AACAGTTCGG CTGGCGCGAG CCCCTGATGC 
     6781 TCTTCGTCCA GATCATCCTG ATCGACAAGA CCGGCTTCCA TCCGAGTACG TGCTCGCTCG 
     6841 ATGCGATGTT TCGCTTGGTG GTCGAATGGG CAGGTAGCCG GATCAAGCGT ATGCAGCCGC 
     6901 CGCATTGCAT CAGCCATGAT GGATACTTTC TCGGCAGGAG CAAGGTGAGA TGACAGGAGA 
     6961 TCCTGCCCCG GCACTTCGCC CAATAGCAGC CAGTCCCTTC CCGCTTCAGT GACAACGTCG 
     7021 AGCACAGCTG CGCAAGGAAC GCCCGTCGTG GCCAGCCACG ATAGCCGCGC TGCCTCGTCT 
     7081 TGCAGTTCAT TCAGGGCACC GGACAGGTCG GTCTTGACAA AAAGAACCGG GCGCCCCTGC 
     7141 GCTGACAGCC GGAACACGGC GGCATCAGAG CAGCCGATTG TCTGTTGTGC CCAGTCATAG 
     7201 CCGAATAGCC TCTCCACCCA AGCGGCCGGA GAACCTGCGT GCAATCCATC TTGTTCAATC 
     7261 ATGCGAAACG ATCCTCATCC TGTCTCTTGA TCGATCTTTG CAAAAGCCTA GGCCTCCAAA 
     7321 AAAGCCTCCT CACTACTTCT GGAATAGCTC AGAGGCCGAG GCGGCCTCGG CCTCTGCATA 
     7381 AATAAAAAAA ATTAGTCAGC CATGGGGCGG AGAATGGGCG GAACTGGGCG GAGTTAGGGG 
     7441 CGGGATGGGC GGAGTTAGGG GCGGGACTAT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     7501 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CTGGTTGCTG ACTAATTGAG 
     7561 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCCTAACTG 
     7621 ACACACATTC CACAGCTGGT TCTTTCCGCC TCAGGACTCT TCCTTTTTCA ATATTATTGA 
     7681 AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT 
     7741 AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGC GCCCTGTAGC 
     7801 GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC 
     7861 GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT 
     7921 CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC 
     7981 CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG 
     8041 ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA 
     8101 ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG 
     8161 ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTT
//