LOCUS       MR211646                8208 bp    DNA     circular  SYN 
DEFINITION  Ubr2 (Myc-DDK-tagged) - Mouse ubiquitin protein ligase E3 component n-recognin 2 (cDNA clone MGC:36320 IMAGE:4935220)
ACCESSION   BC031403
VERSION     BC031403.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4365..4456 
                     /label="Myc-DDK"  
     misc_feature    4486..5071 
                     /label="PolyA signal"  
     misc_feature    4543..4562 
                     /label="Restriction Enzyme"  
     misc_feature    5347..6019 
                     /label="ColE1"  
     misc_feature    6449..7243 
                     /label="Kan/Neo"  
     misc_feature    7278..7580 
                     /label="SV40 ori"  
     misc_feature    7666..8143 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4347..4353 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4347 
                     /label="Ubr2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTTGATAGAA CATCCTCTTA GATGTCTTGT CTTATGTGCT CAAGTGCATG 
     1081 CTGGGATGTG GAGAAGAAAT GGCTTCTCTC TAGTAAATCA GATCTATTAC TACCATAATG 
     1141 TGAAATGCAG GCGAGAGATG TTCGACAAGG ACATAGTGAT GCTTCAGACA GGTGTCTCCA 
     1201 TGATGGACCC AAACCACTTC CTGATGATCA TGCTCAGCCG CTTTGAACTC TATCAGCTCT 
     1261 TCAGCACGCC TGACTATGGG AAGAGATTCA GTTCTGAGGT TACCCATAAG GACGTCGTTC 
     1321 AGCAGAACAA CACTCTGATC GAAGAGATGC TCTACCTCAT CATCATGCTT GTGGGAGAAA 
     1381 GATTCAACCC TGGGGTTGGA CAGGTGGCTG CCACAGATGA AATCAAGAGG GAGATTATCC 
     1441 ATCAGTTGAG CATCAAGCCT ATGGCTCACA GTGAGCTGGT GAAGTCTCTG CCTGAAGATG 
     1501 AGAACAAGGA GACCGGCATG GAGAGCGTCA TCGAGTCCGT TGCACATTTC AAGAAACCTG 
     1561 GGCTCACAGG GCGAGGCATG TATGAGCTGA AGCCAGAGTG TGCCAAAGAG TTCAACCTGT 
     1621 ATTTTTATCA TTTCTCCAGG GCAGAGCAGT CCAAGGCAGA GGAAGCTCAG CGGAAATTGA 
     1681 AAAGAGAAAA TAAAGAAGAT ACAGCACTCC CTCCTCCGGC TTTGCCACCG TTCTGCCCTT 
     1741 TGTTCGCGAG TCTGGTTAAC ATCTTGCAGT GTGACGTCAT GCTGTACATC ATGGGAACGA 
     1801 TCCTGCAGTG GGCTGTAGAG CATCACGGGT CTGCCTGGTC AGAGTCCATG CTACAGAGGG 
     1861 TGCTGCATTT GATCGGGATG GCTCTCCAGG AAGAGAAGCA CCACTTGGAG AACGCCGTGG 
     1921 AAGGGCACGT GCAGACCTTC ACCTTCACAC AGAAGATTTC AAAGCCTGGT GATGCACCAC 
     1981 ATAACTCCCC GAGCATCCTA GCTATGCTGG AGACCTTGCA GAACGCCCCC TCCCTGGAAG 
     2041 CCCACAAGGA CATGATCAGG TGGTTGCTAA AGATGTTTAA TGCAATTAAG AAGATAAGAG 
     2101 AGTGTTCATC CAGCAGCCCT GTGGCCGAGG CGGAGGGAAC CATAATGGAG GAGAGCTCAA 
     2161 GAGACAAGGA CAAAGCAGAG AGGAAAAGAA AAGCCGAGAT CGCCAGACTG CGCCGGGAGA 
     2221 AGATCATGGC CCAGATGTCT GAGATGCAGC GGCACTTCAT TGACGAAAAC AAAGAGCTCT 
     2281 TCCAGCAGAC CCTAGAGCTG GACACCTCTG CCTCTGCCAC TCTTGACAGC AGCCCTCCCG 
     2341 TTTCAGACGC AGCTCTTACA GCACTGGGCC CAGCACAGAC ACAGGTCCCT GAACCGAGAC 
     2401 AGTTTGTCAC CTGTATATTA TGTCAAGAGG AGCAAGAGGT GACTGTGGGA AGCAGGGCGA 
     2461 TGGTCTTGGC AGCGTTTGTT CAGAGGTCAA CGGTTCTGTC AAAAGACAGG ACGAAAACCA 
     2521 TCGCGGACCC AGAAAAATAT GATCCATTAT TCATGCACCC CGATCTGTCT TGTGGGACAC 
     2581 ACACTGGCAG CTGTGGGCAC GTTATGCATG CCCATTGTTG GCAAAGGTAT TTTGATTCCG 
     2641 TTCAAGCCAA GGAGCAGCGA AGGCAGCAGC GGCTGCGCTT GCACACTAGC TACGATGTAG 
     2701 AGAATGGCGA GTTCCTCTGC CCGCTCTGTG AGTGCCTGAG CAACACGGTG ATCCCCCTGC 
     2761 TGCTTCCTCC CAGGAGCATC CTCAGCAGGA GGTTAAATTT TTCAGACCAA CCAGATCTGG 
     2821 CACAGTGGAC GAGAGCAGTA ACACAGCAGA TAAAGGTGGT CCAGATGCTG AGGAGAAAGC 
     2881 ACAATGCTGC TGACACGTCT TCTTCAGAGG ACACAGAAGC CATGAATATA ATACCGATCC 
     2941 CCGAAGGCTT CAGGCCTGAT TTTTATCCTA GGAACCCATA TTCTGATAGC ATAAAAGAAA 
     3001 TGTTAACGAC ATTTGGAACG GCTGCTTACA AGGTGGGACT GAAGGTTCAT CCTAATGAAG 
     3061 GTGACCCCCG TGTGCCCATC CTGTGCTGGG GGACCTGTGC ATACACCATC CAGAGCATAG 
     3121 AAAGAATTTT GAGTGATGAG GAGAAGCCTG TTTTTGGACC TCTGCCTTGT AGACTGGACG 
     3181 ACTGTCTCAG GTCGTTAACA CGGTTTGCAG CAGCACATTG GACAGTGGCG TTACTTCCTG 
     3241 TGGTACAAGG ACACTTCTGT AAACTCTTTG CATCCTTGGT GCCTAGTGAC AGCTATGAAG 
     3301 ACCTCCCGTG CATACTAGAC ATCGACATGT TTCACTTGCT GGTGGGCCTG GTGCTCGCTT 
     3361 TCCCAGCTCT GCAGTGTCAG GATTTTTCAG GAAGCAGCCT GGCCACTGGG GACCTGCACA 
     3421 TCTTCCACTT GGTTACCATG GCACACATCG TACAGATCTT ACTTACCTCA TGTACAGAAG 
     3481 AGAATGGCAT GGATCAAGAG AATCCCACTG GGGAAGAAGA ACTGGCCATT CTCTCTTTGC 
     3541 ACAAAACACT TCACCAGTAT ACTGGAAGTG CCTTGAAAGA AGCCCCCTCC GGCTGGCACC 
     3601 TGTGGAGGAG CGTCCGGGCC GCCATCATGC CTTTCCTCAA GTGCTCTGCT TTGTTTTTCC 
     3661 ACTATTTAAA TGGAGTCCCG GCCCCTCCAG ACCTTCAAGT TTCTGGAACA AGCCATTTTG 
     3721 AACACTTATG TAACTACCTT TCCCTACCAA CCAACCTCAT TCACCTTTTT CAAGAAAACA 
     3781 GTGACATCAT GAACTCCCTG ATTGAAAGTT GGTGCCAGAA CAGTGAAGTT AAACGGTATC 
     3841 TAAATGGCGA GAGAGGAGCG ATAAGCTACC CCAGAGGAGC TAACAAACTG ATAGACCTTC 
     3901 CAGAGGATTA CAGCAGCCTC ATTAACCAAG CATCCAATTT CTCGTGCCCC AAATCAGGTG 
     3961 GCGACAAGAG CAGAGCTCCT ACTCTGTGCC TCGTGTGTGG GAGTCTCCTC TGCTCTCAGA 
     4021 GTTACTGCTG CCAAGCTGAG CTGGAGGGTG AGGACGTCGG AGCCTGCACA GCACACACCT 
     4081 ACTCCTGCGG CTCCGGGGCC GGCATCTTCC TGAGAGTGCG GGAATGTCAG GTGCTATTTT 
     4141 TAGCTGGCAA AACCAAAGGA TGTTTTTATT CTCCTCCTTA CCTTGACGAC TATGGAGAGA 
     4201 CCGACCAGGG ACTCAGACGA GGAAATCCTT TACATTTATG CCAAGAGCGG TTTCGAAAGA 
     4261 TCCAGAAGCT CTGGCAGCAG CATAGTATCA CAGAGGAGAT CGGACACGCG CAGGAGGCTA 
     4321 ACCAGACCCT GGTCGGAATT GACTGGCAGC ATTTAACGCG TACGCGGCCG CTCGAGCAGA 
     4381 AACTCATCTC AGAAGAGGAT CTGGCAGCAA ATGATATCCT GGATTACAAG GATGACGACG 
     4441 ATAAGGTTTA AACGGCCGGC CGCGGTCATA GCTGTTTCCT GAACAGATCC CGGGTGGCAT 
     4501 CCCTGTGACC CCTCCCCAGT GCCTCTCCTG GCCCTGGAAG TTGCCACTCC AGTGCCCACC 
     4561 AGCCTTGTCC TAATAAAATT AAGTTGCATC ATTTTGTCTG ACTAGGTGTC CTTCTATAAT 
     4621 ATTATGGGGT GGAGGGGGGT GGTATGGAGC AAGGGGCAAG TTGGGAAGAC AACCTGTAGG 
     4681 GCCTGCGGGG TCTATTGGGA ACCAAGCTGG AGTGCAGTGG CACAATCTTG GCTCACTGCA 
     4741 ATCTCCGCCT CCTGGGTTCA AGCGATTCTC CTGCCTCAGC CTCCCGAGTT GTTGGGATTC 
     4801 CAGGCATGCA TGACCAGGCT CAGCTAATTT TTGTTTTTTT GGTAGAGACG GGGTTTCACC 
     4861 ATATTGGCCA GGCTGGTCTC CAACTCCTAA TCTCAGGTGA TCTACCCACC TTGGCCTCCC 
     4921 AAATTGCTGG GATTACAGGC GTGAACCACT GCTCCCTTCC CTGTCCTTCT GATTTTAAAA 
     4981 TAACTATACC AGCAGGAGGA CGTCCAGACA CAGCATAGGC TACCTGGCCA TGCCCAACCG 
     5041 GTGGGACATT TGAGTTGCTT GCTTGGCACT GTCCTCTCAT GCGTTGGGTC CACTCAGTAG 
     5101 ATGCCTGTTG AATTGGGTAC GCGGCCAGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT 
     5161 AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA 
     5221 GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC 
     5281 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT 
     5341 ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT 
     5401 GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG 
     5461 CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA 
     5521 CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA 
     5581 CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC 
     5641 GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG 
     5701 AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG 
     5761 TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA 
     5821 GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC 
     5881 TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG 
     5941 GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA 
     6001 TGAGTAACCT GAGGCTATGG CAGGGCCTGC CGCCCCGACG TTGGCTGCGA GCCCTGGGCC 
     6061 TTCACCCGAA CTTGGGGGGT GGGGTGGGGA AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA 
     6121 ATGGGGTCTC GGTGGGGTAT CGACAGAGTG CCAGCCCTGG GACCGAACCC CGCGTTTATG 
     6181 AACAAACGAC CCAACACCGT GCGTTTTATT CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT 
     6241 TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA 
     6301 CTCCAGCATG AGATCCCCGC GCTGGAGGAT CATCCAGCCG GCGTCCCGGA AAACGATTCC 
     6361 GAAGCCCAAC CTTTCATAGA AGGCGGCGGT GGAATCGAAA TCTCGTGATG GCAGGTTGGG 
     6421 CGTCGCTTGG TCGGTCATTT CGAACCCCAG AGTCCCGCTC AGAAGAACTC GTCAAGAAGG 
     6481 CGATAGAAGG CGATGCGCTG CGAATCGGGA GCGGCGATAC CGTAAAGCAC GAGGAAGCGG 
     6541 TCAGCCCATT CGCCGCCAAG CTCTTCAGCA ATATCACGGG TAGCCAACGC TATGTCCTGA 
     6601 TAGCGATCCG CCACACCCAG CCGGCCACAG TCGATGAATC CAGAAAAGCG GCCATTTTCC 
     6661 ACCATGATAT TCGGCAAGCA GGCATCGCCA TGGGTCACGA CGAGATCCTC GCCGTCGGGC 
     6721 ATGCTCGCCT TGAGCCTGGC GAACAGTTCG GCTGGCGCGA GCCCCTGATG CTCTTCGTCC 
     6781 AGATCATCCT GATCGACAAG ACCGGCTTCC ATCCGAGTAC GTGCTCGCTC GATGCGATGT 
     6841 TTCGCTTGGT GGTCGAATGG GCAGGTAGCC GGATCAAGCG TATGCAGCCG CCGCATTGCA 
     6901 TCAGCCATGA TGGATACTTT CTCGGCAGGA GCAAGGTGAG ATGACAGGAG ATCCTGCCCC 
     6961 GGCACTTCGC CCAATAGCAG CCAGTCCCTT CCCGCTTCAG TGACAACGTC GAGCACAGCT 
     7021 GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC GATAGCCGCG CTGCCTCGTC TTGCAGTTCA 
     7081 TTCAGGGCAC CGGACAGGTC GGTCTTGACA AAAAGAACCG GGCGCCCCTG CGCTGACAGC 
     7141 CGGAACACGG CGGCATCAGA GCAGCCGATT GTCTGTTGTG CCCAGTCATA GCCGAATAGC 
     7201 CTCTCCACCC AAGCGGCCGG AGAACCTGCG TGCAATCCAT CTTGTTCAAT CATGCGAAAC 
     7261 GATCCTCATC CTGTCTCTTG ATCGATCTTT GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC 
     7321 TCACTACTTC TGGAATAGCT CAGAGGCCGA GGCGGCCTCG GCCTCTGCAT AAATAAAAAA 
     7381 AATTAGTCAG CCATGGGGCG GAGAATGGGC GGAACTGGGC GGAGTTAGGG GCGGGATGGG 
     7441 CGGAGTTAGG GGCGGGACTA TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     7501 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCTGGTTGCT GACTAATTGA GATGCATGCT 
     7561 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCCTAACT GACACACATT 
     7621 CCACAGCTGG TTCTTTCCGC CTCAGGACTC TTCCTTTTTC AATATTATTG AAGCATTTAT 
     7681 CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA 
     7741 GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG CGCCCTGTAG CGGCGCATTA 
     7801 AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG 
     7861 CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA 
     7921 GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC 
     7981 AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT 
     8041 CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA 
     8101 ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC 
     8161 TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTT
//