LOCUS       MR211642                8199 bp    DNA     circular  SYN 
DEFINITION  Slc12a6 (Myc-DDK-tagged) - Mouse solute carrier family 12, member 6 (cDNA clone MGC:69652 IMAGE:6844732)
ACCESSION   BC062099
VERSION     BC062099.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4356..4447 
                     /label="Myc-DDK"  
     misc_feature    4477..5062 
                     /label="PolyA signal"  
     misc_feature    4534..4553 
                     /label="Restriction Enzyme"  
     misc_feature    5338..6010 
                     /label="ColE1"  
     misc_feature    6440..7234 
                     /label="Kan/Neo"  
     misc_feature    7269..7571 
                     /label="SV40 ori"  
     misc_feature    7657..8134 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4338..4344 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4338 
                     /label="Slc12a6"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCATCCACCA GAAGCCACCA CCAAGATGTC CTCAGTTCGG TTCATGGTGA 
     1081 CACCAACTAA GATTGATGAC ATTCCAGGTT TGTCAGACAC CAGCCCGGAC CTCAGCTCTC 
     1141 GGTCTAGTTC TCGAGTAAGA TTTAGCTCCC GAGAAAGTGT GCCAGAAACA AGCCGTAGTG 
     1201 AGCCTATGAG CGAACTGTCT GGGGCTACTA CTTCTCTGGC AACTGTTGCC CTAGATCCTT 
     1261 CCAGTGACCG GACTTCTAAT CCCCAGGATG TTACGGAGGA TGACGGCCAT AAAAAAGCCC 
     1321 GAAATGCTTA TCTCAATAAT TCCAACTATG AAGAAGGAGA CGAATATTTT GATAAAAATT 
     1381 TGGCACTCTT TGAGGAAGAA ATGGACACCA GACCAAAGGT GTCTTCTCTC CTCAACCGCA 
     1441 TGGCCAACTA TACAAATCTG ACACAAGGAG CAAAGGAACA TGAAGAGGCA GAGAACATCA 
     1501 CTGAAGGGAA AAAGAAGCCT ACCAAGAGCC CCCAAATGGG TACTTTCATG GGTGTCTACC 
     1561 TCCCATGTCT ACAGAACATC TTTGGAGTGA TCCTCTTCCT GCGTCTTACC TGGGTAGTGG 
     1621 GAACAGCTGG AATCCTTCAG GCCTTTGCAA TTGTCCTCAT CTGCTGCTGC TGTACAATGT 
     1681 TAACTGCCAT CTCCATGAGC GCCATCGCCA CTAACGGAGT GGTGCCAGCT GGGGGCTCAT 
     1741 ACTTCATGAT TTCCAGAGCC CTGGGCCCAG AGTTTGGCGG GGCTGTAGGC CTCTGCTTTT 
     1801 ATCTTGGCAC CACATTTGCA GCAGCCATGT ATATTCTTGG TGCCATTGAA ATCTTTCTGG 
     1861 TATACATTGT CCCCCGAGCT GCCATCTTTC GGAGTGACGA TGCACTCAAG GAGTCAGCAG 
     1921 CTATGCTGAA CAACATGCGC GTCTATGGTA CAGCCTTCTT GGTCCTCATG GTCTTGGTGG 
     1981 TATTCATCGG CGTACGCTAT GTGAATAAGT TTGCCTCACT CTTCCTGGCC TGTGTAATTG 
     2041 TGTCGATCTT GGCTATCTAT GCTGGTGCCA TCAAGTCTTC CTTTGCTCCA CCACACTTCC 
     2101 CGGTCTGTAT GCTGGGCAAC CGTACCCTGT CATCAAGACA CCTTGACATT TGCTCTAAGA 
     2161 CCAAGGAGGT TGACAACATG ACAGTACCAT CAAAGTTATG GGGATTCTTC TGCAACTCGA 
     2221 GTCAGTTCTT TAATGCCACC TGTGATGAGT ACTTTGTTCA CAATAACGTC ATCTCAATCC 
     2281 AAGGCATTCC AGGGTTGGCT AGTGGTATCA TTACTGAAAA TCTTTGGAGT AATTATTTAC 
     2341 CAAAGGGTGA GATAATTGAA AAGCCATCAG CCAAGTCATC TGATGTCTTA GGCAACTTAA 
     2401 ACCATGAATA TGTTCTTGCT GATATCACCA CCTCCTTCAC TCTGCTGGTG GGGATCTTCT 
     2461 TTCCCTCGGT CACAGGTATC ATGGCTGGGT CAAACAGATC TGGAGATCTG AAAGATGCCC 
     2521 AGAAGTCTAT TCCCATTGGG ACCATCCTTG CCATCCTGAC CACATCCTTT GTGTATTTAA 
     2581 GCAATGTTGT CCTTTTTGGT GCATGTATTG AAGGAGTCGT TCTCAGAGAC AAATTTGGGG 
     2641 ATGCTGTAAA AGGGAATTTG GTTGTAGGCA CCTTATCCTG GCCATCCCCG TGGGTGATCG 
     2701 TTATTGGCTC CTTCTTTTCA ACATGTGGGG CTGGGCTGCA GAGCCTCACA GGTGCGCCTC 
     2761 GGCTGCTGCA GGCTATCGCC AAGGATAACA TCATACCTTT CCTTAGGGTT TTTGGTCACA 
     2821 GCAAAGCTAA TGGGGAACCT ACCTGGGCTT TACTTCTAAC TGCTGCCATA GCAGAGCTGG 
     2881 GAATTCTCAT CGCCTCCCTG GATCTCGTGG CCCCAATTCT TTCCATGTTT TTTCTCATGT 
     2941 GTTACCTCTT TGTGAACTTG GCTTGTGCCT TGCAAACATT GCTGCGAACC CCCAACTGGA 
     3001 GGCCTCGATT CCGCTATTAT CACTGGGCCC TCTCTTTCAT GGGAATGAGT ATCTGTCTAG 
     3061 CTCTGATGTT CATTTCTTCT TGGTATTATG CCATTGTAGC TATGGTAATA GCTGGCATGA 
     3121 TCTACAAGTA CATTGAATAT CAAGGGGCTG AGAAAGAATG GGGGGATGGT ATCCGTGGGC 
     3181 TGTCGCTCAG TGCAGCCCGC TTCGCTTTGC TCCGTCTAGA GGAAGGACCT CCTCACACTA 
     3241 AAAACTGGAG GCCTCAGCTG CTCGTCCTAC TGAAGCTGGA TGAAGATTTA CACGTCAAGC 
     3301 ACCCTCGCCT CCTCACCTTT GCCTCCCAGC TCAAGGCAGG AAAGGGACTC ACGATTGTGG 
     3361 GCTCTGTCAT CGTGGGGAAC TTCTTGGAGA ACTATGGTGA CGCGCTCGCG GCAGAGCAGA 
     3421 CCATTAAGCA CCTAATGGAG GCAGAAAAGG TAAAAGGATT CTGCCAATTG GTGGTGGCTG 
     3481 CCAAGCTGAA AGAGGGCATT TCACACCTCA TCCAGTCCTG TGGCCTCGGA GGCATGAAAC 
     3541 ACAACACAGT GGTGATGGGC TGGCCCAATG GCTGGCGTCA GAGTGAAGAT GCTCGCGCTT 
     3601 GGAAGACTTT CATTGGCACA GTACGAGTGA CAACTGCTGC CCATCTAGCC CTGCTGGTGG 
     3661 CTAAAAATGT CTCCTTCTTT CCCAGCAATG TGGAGCAGTT TTCTGAGGGC AACATTGATG 
     3721 TGTGGTGGAT TGTGCATGAT GGGGGCATGC TCATGCTATT ACCGTTCCTG CTGAAACAGC 
     3781 ACAAGGTTTG GCGGAAATGC AGCATACGGA TCTTCACAGT AGCCCAACTA GAAGACAACA 
     3841 GTATCCAGAT GAAGAAGGAT CTGGCCACCT TTCTGTACCA CCTGCGCATT GAGGCAGAAG 
     3901 TGGAAGTGGT GGAGATGCAC GACAGTGACA TATCTGCCTA TACATATGAG CGCACCCTGA 
     3961 TGATGGAGCA GAGGTCCCAG ATGCTTCGGC ATATGCGGCT GTCCAAAACA GAGCGAGACA 
     4021 GGGAGGCACA GCTGGTGAAA GATCGAAACT CAATGCTACG CTTGACCAGC ATTGGCTCTG 
     4081 ATGAGGACGA AGAGACAGAA ACGTACCAGG AGAAGGTGCA CATGACTTGG ACCAAGGATA 
     4141 AATACATGGC ATCCCGGGGG CAAAAGGTCA AGTCAATGGA AGGATTCCAG GACCTACTTA 
     4201 ATATGCGTCC GGACCAGTCC AACGTGAGAC GGATGCATAC AGCAGTGAAG CTCAATGAAG 
     4261 TTATAGTCAA CAAGTCTCAT GAAGCAAAGC TGGTTTTGTT GAATATGCCA GGACCACCCC 
     4321 GGAACCCTGA AGGTGATGAA AACTGCACGC GTACGCGGCC GCTCGAGCAG AAACTCATCT 
     4381 CAGAAGAGGA TCTGGCAGCA AATGATATCC TGGATTACAA GGATGACGAC GATAAGGTTT 
     4441 AAACGGCCGG CCGCGGTCAT AGCTGTTTCC TGAACAGATC CCGGGTGGCA TCCCTGTGAC 
     4501 CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA GTTGCCACTC CAGTGCCCAC CAGCCTTGTC 
     4561 CTAATAAAAT TAAGTTGCAT CATTTTGTCT GACTAGGTGT CCTTCTATAA TATTATGGGG 
     4621 TGGAGGGGGG TGGTATGGAG CAAGGGGCAA GTTGGGAAGA CAACCTGTAG GGCCTGCGGG 
     4681 GTCTATTGGG AACCAAGCTG GAGTGCAGTG GCACAATCTT GGCTCACTGC AATCTCCGCC 
     4741 TCCTGGGTTC AAGCGATTCT CCTGCCTCAG CCTCCCGAGT TGTTGGGATT CCAGGCATGC 
     4801 ATGACCAGGC TCAGCTAATT TTTGTTTTTT TGGTAGAGAC GGGGTTTCAC CATATTGGCC 
     4861 AGGCTGGTCT CCAACTCCTA ATCTCAGGTG ATCTACCCAC CTTGGCCTCC CAAATTGCTG 
     4921 GGATTACAGG CGTGAACCAC TGCTCCCTTC CCTGTCCTTC TGATTTTAAA ATAACTATAC 
     4981 CAGCAGGAGG ACGTCCAGAC ACAGCATAGG CTACCTGGCC ATGCCCAACC GGTGGGACAT 
     5041 TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA TGCGTTGGGT CCACTCAGTA GATGCCTGTT 
     5101 GAATTGGGTA CGCGGCCAGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT 
     5161 ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC 
     5221 CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA 
     5281 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA 
     5341 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC 
     5401 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG 
     5461 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC 
     5521 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG 
     5581 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT 
     5641 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT 
     5701 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG 
     5761 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC 
     5821 GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA 
     5881 GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC 
     5941 CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAACC 
     6001 TGAGGCTATG GCAGGGCCTG CCGCCCCGAC GTTGGCTGCG AGCCCTGGGC CTTCACCCGA 
     6061 ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA AACGCGGGCG TATTGGCCCC AATGGGGTCT 
     6121 CGGTGGGGTA TCGACAGAGT GCCAGCCCTG GGACCGAACC CCGCGTTTAT GAACAAACGA 
     6181 CCCAACACCG TGCGTTTTAT TCTGTCTTTT TATTGCCGTC ATAGCGCGGG TTCCTTCCGG 
     6241 TATTGTCTCC TTCCGTGTTT CAGTTAGCCT CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT 
     6301 GAGATCCCCG CGCTGGAGGA TCATCCAGCC GGCGTCCCGG AAAACGATTC CGAAGCCCAA 
     6361 CCTTTCATAG AAGGCGGCGG TGGAATCGAA ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG 
     6421 GTCGGTCATT TCGAACCCCA GAGTCCCGCT CAGAAGAACT CGTCAAGAAG GCGATAGAAG 
     6481 GCGATGCGCT GCGAATCGGG AGCGGCGATA CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT 
     6541 TCGCCGCCAA GCTCTTCAGC AATATCACGG GTAGCCAACG CTATGTCCTG ATAGCGATCC 
     6601 GCCACACCCA GCCGGCCACA GTCGATGAAT CCAGAAAAGC GGCCATTTTC CACCATGATA 
     6661 TTCGGCAAGC AGGCATCGCC ATGGGTCACG ACGAGATCCT CGCCGTCGGG CATGCTCGCC 
     6721 TTGAGCCTGG CGAACAGTTC GGCTGGCGCG AGCCCCTGAT GCTCTTCGTC CAGATCATCC 
     6781 TGATCGACAA GACCGGCTTC CATCCGAGTA CGTGCTCGCT CGATGCGATG TTTCGCTTGG 
     6841 TGGTCGAATG GGCAGGTAGC CGGATCAAGC GTATGCAGCC GCCGCATTGC ATCAGCCATG 
     6901 ATGGATACTT TCTCGGCAGG AGCAAGGTGA GATGACAGGA GATCCTGCCC CGGCACTTCG 
     6961 CCCAATAGCA GCCAGTCCCT TCCCGCTTCA GTGACAACGT CGAGCACAGC TGCGCAAGGA 
     7021 ACGCCCGTCG TGGCCAGCCA CGATAGCCGC GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA 
     7081 CCGGACAGGT CGGTCTTGAC AAAAAGAACC GGGCGCCCCT GCGCTGACAG CCGGAACACG 
     7141 GCGGCATCAG AGCAGCCGAT TGTCTGTTGT GCCCAGTCAT AGCCGAATAG CCTCTCCACC 
     7201 CAAGCGGCCG GAGAACCTGC GTGCAATCCA TCTTGTTCAA TCATGCGAAA CGATCCTCAT 
     7261 CCTGTCTCTT GATCGATCTT TGCAAAAGCC TAGGCCTCCA AAAAAGCCTC CTCACTACTT 
     7321 CTGGAATAGC TCAGAGGCCG AGGCGGCCTC GGCCTCTGCA TAAATAAAAA AAATTAGTCA 
     7381 GCCATGGGGC GGAGAATGGG CGGAACTGGG CGGAGTTAGG GGCGGGATGG GCGGAGTTAG 
     7441 GGGCGGGACT ATGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     7501 GGAGCCTGGG GACTTTCCAC ACCTGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     7561 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCCTAAC TGACACACAT TCCACAGCTG 
     7621 GTTCTTTCCG CCTCAGGACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT 
     7681 TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG 
     7741 CGCACATTTC CCCGAAAAGT GCCACCTGAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG 
     7801 GGTGTGGTGG TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT 
     7861 TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT 
     7921 CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT 
     7981 GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG 
     8041 ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC 
     8101 CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA 
     8161 AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTTT
//