LOCUS       MR211635                8181 bp    DNA     circular  SYN 
DEFINITION  Jak3 (Myc-DDK-tagged) - Mouse Janus kinase 3 (Jak3), transcript variant 1
ACCESSION   NM_010589
VERSION     NM_010589.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4338..4429 
                     /label="Myc-DDK"  
     misc_feature    4459..5044 
                     /label="PolyA signal"  
     misc_feature    4516..4535 
                     /label="Restriction Enzyme"  
     misc_feature    5320..5992 
                     /label="ColE1"  
     misc_feature    6422..7216 
                     /label="Kan/Neo"  
     misc_feature    7251..7553 
                     /label="SV40 ori"  
     misc_feature    7639..8116 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4320..4326 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4320 
                     /label="Jak3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCACCTCCA AGTGAGGAGA CACCTCTGAT CCCTCAGCGC TCTTGCAGCC 
     1081 TCTCATCCTC AGAGGCAGGA GCCCTGCATG TGCTCCTTCC TCCCCGGGGA CCTGGGCCTC 
     1141 CCCAGCGATT GTCATTCTCT TTTGGGGACT ACTTGGCTGA GGATTTATGT GTGCGAGCTG 
     1201 CCAAGGCCTG TGGCATCCTG CCTGTTTATC ATTCGCTTTT CGCTCTGGCC ACTGAGGACT 
     1261 TCTCTTGCTG GTTTCCCCCA AGCCACATCT TCTGCATAGA GGACGTGGAC ACTCAAGTCT 
     1321 TGGTCTACAG GCTACGCTTT TATTTCCCTG ACTGGTTTGG GCTGGAGACA TGTCACCGCT 
     1381 TTGGGCTGCG CAAAGATTTG ACCAGTGCCA TCCTTGACTT ACACGTTTTA GAACATCTCT 
     1441 TTGCTCAGCA CCGCAGTGAC CTGGTGAGTG GGCGCCTCCC GGTGGGCCTT AGCATGAAGG 
     1501 AGCAGGGAGA GTTCCTGAGC CTGGCCGTGC TGGACTTGGC CCAGATGGCT CGTGAGCAGG 
     1561 CCCAGCGCCC AGGAGAGCTG CTGAAGACGG TCAGTTACAA AGCCTGTCTG CCGCCCAGCC 
     1621 TGCGCGATGT GATCCAGGGC CAGAACTTCG TGACACGCAG GCGCATCCGC AGGACCGTGG 
     1681 TCTTGGCGCT GCGCCGTGTG GTCGCCTGCC AGGCCGACCG CTACGCGCTC ATGGCCAAGT 
     1741 ATATTTTGGA CCTGGAGCGG CTACATCCAG CGGCCACCAC CGAGACCTTC CGTGTGGGGC 
     1801 TCCCGGGCGC CCAGGAGGAG CCGGGGCTTC TGCGTGTGGC GGGGGACAAC GGCATCTCCT 
     1861 GGAGCTCCGG GGACCAGGAG CTTTTCCAGA CCTTCTGTGA CTTTCCGGAA ATCGTGGATG 
     1921 TCAGCATCAA GCAGGCCCCA CGTGTGGGTC CGGCAGGGGA ACACCGGCTG GTCACCGTCA 
     1981 CCAGGATGGA CGGCCACATC CTGGAAGCGG AGTTTCCGGG GCTGCCTGAG GCGCTGTCCT 
     2041 TCGTGGCCCT TGTGGATGGG TACTTCCGCC TGATCTGCGA CTCCAGGCAT TATTTCTGCA 
     2101 AGGAGGTGGC GCCGCCGCGG CTGCTGGAGG AGGAGGCGGA GCTGTGCCAT GGACCCATCA 
     2161 CGTTAGACTT TGCCATCCAC AAGCTGAAGG CCGCTGGCTC CCTCCCAGGC ACCTACATTC 
     2221 TCCGCCGCAG CCCGCAGGAC TATGACAGCT TTCTTCTTAC CGCCTGCGTC CAGACTCCTC 
     2281 TTGGCCCCGA CTACAAGGGC TGCCTCATCC GCCAGGACCC CAGCGGGGCT TTCTCCCTGG 
     2341 TTGGCCTCAG CCAGCCCCAC AGAAGCCTGC GGGAGCTGCT TGCAGCCTGC TGGAATTCTG 
     2401 GGCTGCGAGT AGACGGTGCT GCCCTGAACC TAACATCCTG CTGCGCTCCC AGACCCAAGG 
     2461 AAAAGTCCAA TTTGATCGTG GTGCGAAGGG GCTGCACCCC CGCGCCTGCC CCTGGCTGCT 
     2521 CCCCGTCCTG CTGTGCGCTG ACACAGCTGA GCTTCCACAC AATTCCAACG GACAGCCTGG 
     2581 AGTGGCACGA GAACCTGGGT CACGGTTCTT TTACCAAGAT CTTCCGTGGC CGCAGGCGGG 
     2641 AGGTCGTGGA TGGTGAGACA CATGACTCGG AAGTCCTCCT GAAGGTCATG GACTCCAGAC 
     2701 ATCGGAACTG CATGGAGTCT TTTCTGGAAG CCGCAAGCTT GATGAGCCAA GTATCCTACC 
     2761 CGCACCTGGT GTTACTGCAC GGCGTCTGCA TGGCTGGAGA CAGCATCATG GTGCAGGAAT 
     2821 TTGTGTATCT AGGAGCAATT GACATGTACC TGCGCAAGCG TGGCCACCTG GTGTCAGCCA 
     2881 GCTGGAAACT GCAGGTGACC AAGCAGCTGG CATATGCCCT TAACTACTTG GAGGACAAAG 
     2941 GCCTTCCTCA CGGCAACGTC TCAGCACGGA AGGTGCTCCT GGCTCGTGAG GGGGGTGATG 
     3001 GGAATCCACC TTTCATTAAG CTGAGTGATC CTGGTGTCAG TCCCACTGTG CTGAGCCTGG 
     3061 AAATGCTCAC CGACAGAATA CCCTGGGTGG CCCCCGAATG TCTCCAGGAG GCTCAGACAC 
     3121 TCGGCTTGGA GGCTGACAAG TGGGGCTTTG GAGCCACCAC GTGGGAGGTG TTCAGCGGGG 
     3181 GACCCGCCCA CATCACCTCG CTGGAGCCCG CCAAAAAGCT GAAGTTCTAT GAGGACCAGG 
     3241 GACAGCTGCC CGCTCTCAAA TGGACAGAAC TGGCGGGACT TATCACACAG TGCATGGCCT 
     3301 ATGATCCTGG CCGGCGCCCC TCCTTCCGAG CTATCCTCAG AGACCTCAAC GGCCTCATTA 
     3361 CATCAGATTA CGAGCTCCTC TCAGACCCCA CACCTGGCAT CCCGAGTCCT CGAGATGAGC 
     3421 TGTGCGGTGG CGCCCAGCTC TATGCCTGCC AGGACCCCGC CATATTCGAG GAGAGACACC 
     3481 TTAAGTACAT CTCTTTGCTG GGCAAGGGCA ACTTTGGCAG CGTGGAGCTG TGCCGCTATG 
     3541 ACCCCCTGGG GGACAATACG GGACCCCTGG TGGCAGTGAA ACAGCTACAG CACAGCGGGC 
     3601 CAGACCAGCA GAGGGACTTC CAGCGGGAGA TTCAGATCCT TAAGGCTCTG CACAGCGACT 
     3661 TCATCGTCAA GTACCGGGGA GTCAGCTATG GGCCAGGTCG CCAGAGCCTG CGGTTGGTGA 
     3721 TGGAGTACCT GCCCAGCGGC TGCCTGCGAG ACTTCCTGCA GCGCCATCGC GCGCGCCTGC 
     3781 ACACCGACCG CCTACTGCTG TTCGCTTGGC AGATCTGCAA GGGCATGGAG TACCTGGGTG 
     3841 CGCGCCGCTG CGTACACCGT GACCTGGCTG CGCGCAACAT CTTGGTGGAG AGCGAGGCTC 
     3901 ATGTGAAGAT CGCGGACTTC GGCCTCGCTA AGCTGCTGCC CCTGGGAAAG GACTACTACG 
     3961 TGGTCCGCGA GCCTGGCCAA AGCCCCATCT TTTGGTATGC CCCGGAGTCC CTATCTGACA 
     4021 ACATCTTCTC CCGCCAATCT GACGTGTGGA GCTTCGGAGT GGTGTTGTAC GAGCTCTTCA 
     4081 CCTACTGCGA CAAGAGCTGC AGCCCATCCG CTGAGTTCCT GAGCATGATG GGGCCTGAGC 
     4141 GTGAAGGACC CCCGCTCTGC CGCCTCCTGG AGCTGCTGGC AGAGGGCCGA CGCCTCCCAC 
     4201 CACCTCCCAC CTGCCCCACC GAGGTTCAGG AGCTCATGCA GCTGTGCTGG GCGCCCAGCC 
     4261 CGCACGACCG GCCAGCCTTC GGCACCCTGA GCCCCCAGCT GGACGCGCTG TGGCGTGGAA 
     4321 GACCCGGAAC GCGTACGCGG CCGCTCGAGC AGAAACTCAT CTCAGAAGAG GATCTGGCAG 
     4381 CAAATGATAT CCTGGATTAC AAGGATGACG ACGATAAGGT TTAAACGGCC GGCCGCGGTC 
     4441 ATAGCTGTTT CCTGAACAGA TCCCGGGTGG CATCCCTGTG ACCCCTCCCC AGTGCCTCTC 
     4501 CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC ACCAGCCTTG TCCTAATAAA ATTAAGTTGC 
     4561 ATCATTTTGT CTGACTAGGT GTCCTTCTAT AATATTATGG GGTGGAGGGG GGTGGTATGG 
     4621 AGCAAGGGGC AAGTTGGGAA GACAACCTGT AGGGCCTGCG GGGTCTATTG GGAACCAAGC 
     4681 TGGAGTGCAG TGGCACAATC TTGGCTCACT GCAATCTCCG CCTCCTGGGT TCAAGCGATT 
     4741 CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA TTCCAGGCAT GCATGACCAG GCTCAGCTAA 
     4801 TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC ACCATATTGG CCAGGCTGGT CTCCAACTCC 
     4861 TAATCTCAGG TGATCTACCC ACCTTGGCCT CCCAAATTGC TGGGATTACA GGCGTGAACC 
     4921 ACTGCTCCCT TCCCTGTCCT TCTGATTTTA AAATAACTAT ACCAGCAGGA GGACGTCCAG 
     4981 ACACAGCATA GGCTACCTGG CCATGCCCAA CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC 
     5041 ACTGTCCTCT CATGCGTTGG GTCCACTCAG TAGATGCCTG TTGAATTGGG TACGCGGCCA 
     5101 GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA 
     5161 TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC 
     5221 CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG 
     5281 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG 
     5341 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT 
     5401 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT 
     5461 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG 
     5521 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT 
     5581 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT 
     5641 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT 
     5701 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC 
     5761 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC 
     5821 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG 
     5881 TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA 
     5941 AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA CCTGAGGCTA TGGCAGGGCC 
     6001 TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG GCCTTCACCC GAACTTGGGG GGTGGGGTGG 
     6061 GGAAAAGGAA GAAACGCGGG CGTATTGGCC CCAATGGGGT CTCGGTGGGG TATCGACAGA 
     6121 GTGCCAGCCC TGGGACCGAA CCCCGCGTTT ATGAACAAAC GACCCAACAC CGTGCGTTTT 
     6181 ATTCTGTCTT TTTATTGCCG TCATAGCGCG GGTTCCTTCC GGTATTGTCT CCTTCCGTGT 
     6241 TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA GAACTCCAGC ATGAGATCCC CGCGCTGGAG 
     6301 GATCATCCAG CCGGCGTCCC GGAAAACGAT TCCGAAGCCC AACCTTTCAT AGAAGGCGGC 
     6361 GGTGGAATCG AAATCTCGTG ATGGCAGGTT GGGCGTCGCT TGGTCGGTCA TTTCGAACCC 
     6421 CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA AGGCGATAGA AGGCGATGCG CTGCGAATCG 
     6481 GGAGCGGCGA TACCGTAAAG CACGAGGAAG CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA 
     6541 GCAATATCAC GGGTAGCCAA CGCTATGTCC TGATAGCGAT CCGCCACACC CAGCCGGCCA 
     6601 CAGTCGATGA ATCCAGAAAA GCGGCCATTT TCCACCATGA TATTCGGCAA GCAGGCATCG 
     6661 CCATGGGTCA CGACGAGATC CTCGCCGTCG GGCATGCTCG CCTTGAGCCT GGCGAACAGT 
     6721 TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG TCCAGATCAT CCTGATCGAC AAGACCGGCT 
     6781 TCCATCCGAG TACGTGCTCG CTCGATGCGA TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA 
     6841 GCCGGATCAA GCGTATGCAG CCGCCGCATT GCATCAGCCA TGATGGATAC TTTCTCGGCA 
     6901 GGAGCAAGGT GAGATGACAG GAGATCCTGC CCCGGCACTT CGCCCAATAG CAGCCAGTCC 
     6961 CTTCCCGCTT CAGTGACAAC GTCGAGCACA GCTGCGCAAG GAACGCCCGT CGTGGCCAGC 
     7021 CACGATAGCC GCGCTGCCTC GTCTTGCAGT TCATTCAGGG CACCGGACAG GTCGGTCTTG 
     7081 ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC AGCCGGAACA CGGCGGCATC AGAGCAGCCG 
     7141 ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT AGCCTCTCCA CCCAAGCGGC CGGAGAACCT 
     7201 GCGTGCAATC CATCTTGTTC AATCATGCGA AACGATCCTC ATCCTGTCTC TTGATCGATC 
     7261 TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC TCCTCACTAC TTCTGGAATA GCTCAGAGGC 
     7321 CGAGGCGGCC TCGGCCTCTG CATAAATAAA AAAAATTAGT CAGCCATGGG GCGGAGAATG 
     7381 GGCGGAACTG GGCGGAGTTA GGGGCGGGAT GGGCGGAGTT AGGGGCGGGA CTATGGTTGC 
     7441 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     7501 ACACCTGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     7561 TGGGGACTTT CCACACCCTA ACTGACACAC ATTCCACAGC TGGTTCTTTC CGCCTCAGGA 
     7621 CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC 
     7681 ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA 
     7741 GTGCCACCTG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC 
     7801 AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC 
     7861 TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG 
     7921 TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA 
     7981 CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC 
     8041 TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT 
     8101 TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA 
     8161 CAAAAATTTA ACGCGAATTT T
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