LOCUS       MR211634                8181 bp    DNA     circular  SYN 
DEFINITION  Arhgef10 (Myc-DDK-tagged) - Mouse Rho guanine nucleotide exchange factor (GEF) 10 (cDNA clone MGC:66545 IMAGE:6400425)
ACCESSION   BC059212
VERSION     BC059212.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4338..4429 
                     /label="Myc-DDK"  
     misc_feature    4459..5044 
                     /label="PolyA signal"  
     misc_feature    4516..4535 
                     /label="Restriction Enzyme"  
     misc_feature    5320..5992 
                     /label="ColE1"  
     misc_feature    6422..7216 
                     /label="Kan/Neo"  
     misc_feature    7251..7553 
                     /label="SV40 ori"  
     misc_feature    7639..8116 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4320..4326 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4320 
                     /label="Arhgef10"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAAAATCCA GAGGAAGCAA TTTACGACGA CGTTCCGAGG GAAAACTCGG 
     1081 ACTCTGAGCC AGATGAAATG ATTTATGATG ATGTGGAGAA CGGCGAAGAA GGTGGAAACA 
     1141 GCTCCCCAGA GTACGGGTGG AGTTCCAGCG AATTCGAAAG CTATGAGGAG CCAAGCGACT 
     1201 CGGAGGGCAA GAACGGAATT CCCAGGTCCT TCCTGCGCAG CAGTCATAAA AAGCAAATGC 
     1261 AGAAGCTTAT GAAGGCAGCC AAGGAGGGCA CCAAAGATGG GCTGGAGAAA ACCAAAGCTG 
     1321 CTGTGAAGAG AGGCGGCTCC TTCATCAGGA CCAGGTCCTT GGTCTCTCAA GATCACAGGT 
     1381 GTTATTTTGA AGAAGAGCAG AATTTGTTCA TTGACGTGGA CTGCAAGCAC CCGGAGGCTG 
     1441 TCCTCACCCC AATGCCCGAG GGCTTGTCTC AGCAGCAGGT TGTGAGAAGA TACATTCTGG 
     1501 GCTCCATTGT TGAGAGCGAG AAGAACTATG TAGATGCTCT CCGGAGGATT TTGGAGCAAT 
     1561 ATGAGAAGCC GCTGTCTGAG ATGGAGCCCC GGCTCCTGAG TGACAGGAAG CTCAGGATGG 
     1621 TTTTCTACCG CGTCAAGGAG ATCCTGCAGT GTCACTCCAT GTTCCAGATT GCGCTGGCCA 
     1681 GCCGCGTGTC CGAGTGGGAT GTCGTGGAAA CAATCGGCGA CGTTTTTGTG GCCTCGTTCT 
     1741 CCAAGTCCAT GGTGCTGGAT GCTTACAGCG AGTACGTGAA CAACTTCAGC ACGGCCGTGG 
     1801 CTGTCCTCAA GAAGACGTGT GCCACAAAGC CTGCTTTCCT TGAGTTCTTA AAGCTGAGCC 
     1861 AGGATTCCAG TCCAGACCGA GTCACACTCC ACAGCCTTAT GATGCGGCCC ATCCAGAGGT 
     1921 TCCCACAGTT CATCCTCCTA CTGCAGGACA TGCTGAAGAA CACCGCCAAA GGACATCCTG 
     1981 ACAGGCTGCC CCTTCAGATG GCGCTGACGG AGCTCGAGAC CCTGGCAGAG AAGCTAAACG 
     2041 AGAGAAAAAG AGATGCCGAC CAGCGATGCG AAATCAAACA AATAGCAAAA GCCATAAACG 
     2101 AGAGATACCT GAACAAGCTC CTCAGCAGTG GAAACCGGTA CCTCGTCCGG TCCGATGACG 
     2161 TCATCGAGAC GGTTTACAAC GACCGGGGAG AAATCGTCAA GACCAAACAA CGCCGGATCT 
     2221 TCATGCTGAA TGACGTGCTC ATGTGTGCCA CCGCCAGCTC ACGGAACTCA CATGAAAGCC 
     2281 ATGCCGTGAT GAGCCAGAGG TACCTGCTCA AGTGGAGTGT CCCTCTAGGT CACGTGGATG 
     2341 TGATTCAGTA CAATGGAGGC TCTGGCGCCG GGGAGCACTG TCGGCACCAC GCTGCACACT 
     2401 CACCTGAGAG CCTGGCCGTG GTGGCCAACG CGAAGCCACA CAAAGTTTAC ATGGGGCCAG 
     2461 GGCAGCTGTA CCAGGATCTG CAGAACCTAC TGCACGACCT GAACGTTGTT GGCCAGATCT 
     2521 CGCAGCTGAT AGGAAACCTC CGAGGGAGCT ATCAGAACTT AAACCAGTCT GTAGCCCATG 
     2581 ATTGGACGTC GGGCTTACAG CGGCTGATAC TGAGGAAAGA AGATGCGATC AGAGCTGCCG 
     2641 ACCGCTGCCG GATCCAGTTA CAGCTCCCGG GAAAGCAGGA CAAGTCCGGG CGGCCCACGT 
     2701 TCTTCACTGC TGTGTTCAAT ACGCTTACAC CAGCCATCAA GGAGTCCTGG GTCAGCAGCC 
     2761 TGCAGATGGC CAAGCTGGCC CTTGAGGAGG AGAACCATAT GGGATGGTTC TGTGTGGATG 
     2821 ACGATGGCAA CCTAGCCAAG AAGGAGACAC ACCCTCTCCT GGTGGGACAC ATGCCTGTGA 
     2881 TGGTGGCCAA GCAGCCAGAG TTCAAGATTG AATGTGCAGC TTATAACCCT GAACCTTACC 
     2941 TGAGCAATGA AAGTCAACCA GCCTCACCCT CCACAGCCCG TGGCTTTCTG TGGATTGGAA 
     3001 GCTGTAGCAA CCAGATGGGT CAGGTCGCCA TTGTCTCCTT CCAAGGTTCC AACCCCAAAG 
     3061 TCATCGAGTG CTTCAATGTG GAATCACGCA TCCTGTGTAT GGTATACATT CCCGCAGAGG 
     3121 AGTCAAAGCC CCAAGAAACC ACCGAAACCA AGGATCCAGA AGCCACAGCC TCGAGAGCAC 
     3181 CCCATGTGCC CACCATCTGC CTGGGCACAG AAGAGGGAAG CATTTCCATT TACAAAAGCA 
     3241 GTCAGGGCTG CAAGAAGGTG AGGCTTCAGC ACTTCTATGC ACCGGACAAG TCCACGGTCA 
     3301 TGAGCTTGGC CTGCTCCCCA CAAGGCCTGT ATGCAGGCCT GGTCAATGGG TCCGTAGCCA 
     3361 GCTACACCAA GGCTCCAGAT GGATCCTGGA ACTCGGAACC CCAGCAAGTG ATCAAGTTGG 
     3421 GTGTCCTGCC AGTCAGAAGC CTTCTCCTGG TGGAGGGTGC ACTGTGGGCA GCCTCGGGAG 
     3481 GGCAGGTGTT CATGGCCAGC GTGGAGACTC ACACTATAGA GAATCAGCTG GAGGCCCACC 
     3541 AAGATGAAGG GATGGTCATC TCCCACATGG CTGTGGCTGG CGTTGGCATC TGGATTGCCT 
     3601 TCACCTCGGG GTCCACCCTC CGCCTCTTCC ACACAGAGAC CTTGAAGCAC CTGCAGGATG 
     3661 TCAACATTGA TGCCCCTGTG CATAGCATGC TGCCAGGGCA CCAGCGTCTA TCTGTGACCA 
     3721 GCCTGCTCGT CTGCCACGGC TTGCTCATGG TCGGTACCAG CCTGGGTGTC GTGGTGGCCC 
     3781 TGCCAGTCCC TCGGCTTCAG GGTATCCCCA AAGTGACAGG GCGAGGCATG GTCTCCTACC 
     3841 ATGCGCATAA CGGGCCCGTT AAGTTCATCG TCTCAGCCAC GGCGTTTCAA AACAAGGACA 
     3901 GAGCCAGGGA CAGCCCACGT TCCGGTTCTG AGCTTCAGGA TGAAGACCCC AAGGACTTGC 
     3961 TCTGCAGCGA GGAAGGTCCT TCCTGCCCGG GCCAGCCTGA CACCTACACA TCCGTTTGGT 
     4021 TGGGAGACTC CCTAGGATTG CCAACTCAAA AGAATGATCT GTCTTCGTCC TCGGGGTCAC 
     4081 TGAACCTGTC CCATGGCTCC AGTTCCCTAG AGCACAGATC TGTGGACAGC AGTCTGTGTG 
     4141 ACCTGCTGAG GGACCCCAGT GCCTCCCCAA GGAGCAGACC ACAGGGCTCT AGGCGAGCAA 
     4201 GAGCCAGTTC AGCGCTGGTG GTATGTGGGG GCCAGGGCCA CCGGCGGGTG CACAGGAAGG 
     4261 CCAGGCAGCC TTCTCAGGAG GACCTGGTTT CTTCAGTGAT GGTGTGGCAG ATCCCCCTGC 
     4321 TGGGCATGAC GCGTACGCGG CCGCTCGAGC AGAAACTCAT CTCAGAAGAG GATCTGGCAG 
     4381 CAAATGATAT CCTGGATTAC AAGGATGACG ACGATAAGGT TTAAACGGCC GGCCGCGGTC 
     4441 ATAGCTGTTT CCTGAACAGA TCCCGGGTGG CATCCCTGTG ACCCCTCCCC AGTGCCTCTC 
     4501 CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC ACCAGCCTTG TCCTAATAAA ATTAAGTTGC 
     4561 ATCATTTTGT CTGACTAGGT GTCCTTCTAT AATATTATGG GGTGGAGGGG GGTGGTATGG 
     4621 AGCAAGGGGC AAGTTGGGAA GACAACCTGT AGGGCCTGCG GGGTCTATTG GGAACCAAGC 
     4681 TGGAGTGCAG TGGCACAATC TTGGCTCACT GCAATCTCCG CCTCCTGGGT TCAAGCGATT 
     4741 CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA TTCCAGGCAT GCATGACCAG GCTCAGCTAA 
     4801 TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC ACCATATTGG CCAGGCTGGT CTCCAACTCC 
     4861 TAATCTCAGG TGATCTACCC ACCTTGGCCT CCCAAATTGC TGGGATTACA GGCGTGAACC 
     4921 ACTGCTCCCT TCCCTGTCCT TCTGATTTTA AAATAACTAT ACCAGCAGGA GGACGTCCAG 
     4981 ACACAGCATA GGCTACCTGG CCATGCCCAA CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC 
     5041 ACTGTCCTCT CATGCGTTGG GTCCACTCAG TAGATGCCTG TTGAATTGGG TACGCGGCCA 
     5101 GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA 
     5161 TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC 
     5221 CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG 
     5281 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG 
     5341 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT 
     5401 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT 
     5461 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG 
     5521 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT 
     5581 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT 
     5641 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT 
     5701 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC 
     5761 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC 
     5821 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG 
     5881 TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA 
     5941 AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA CCTGAGGCTA TGGCAGGGCC 
     6001 TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG GCCTTCACCC GAACTTGGGG GGTGGGGTGG 
     6061 GGAAAAGGAA GAAACGCGGG CGTATTGGCC CCAATGGGGT CTCGGTGGGG TATCGACAGA 
     6121 GTGCCAGCCC TGGGACCGAA CCCCGCGTTT ATGAACAAAC GACCCAACAC CGTGCGTTTT 
     6181 ATTCTGTCTT TTTATTGCCG TCATAGCGCG GGTTCCTTCC GGTATTGTCT CCTTCCGTGT 
     6241 TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA GAACTCCAGC ATGAGATCCC CGCGCTGGAG 
     6301 GATCATCCAG CCGGCGTCCC GGAAAACGAT TCCGAAGCCC AACCTTTCAT AGAAGGCGGC 
     6361 GGTGGAATCG AAATCTCGTG ATGGCAGGTT GGGCGTCGCT TGGTCGGTCA TTTCGAACCC 
     6421 CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA AGGCGATAGA AGGCGATGCG CTGCGAATCG 
     6481 GGAGCGGCGA TACCGTAAAG CACGAGGAAG CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA 
     6541 GCAATATCAC GGGTAGCCAA CGCTATGTCC TGATAGCGAT CCGCCACACC CAGCCGGCCA 
     6601 CAGTCGATGA ATCCAGAAAA GCGGCCATTT TCCACCATGA TATTCGGCAA GCAGGCATCG 
     6661 CCATGGGTCA CGACGAGATC CTCGCCGTCG GGCATGCTCG CCTTGAGCCT GGCGAACAGT 
     6721 TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG TCCAGATCAT CCTGATCGAC AAGACCGGCT 
     6781 TCCATCCGAG TACGTGCTCG CTCGATGCGA TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA 
     6841 GCCGGATCAA GCGTATGCAG CCGCCGCATT GCATCAGCCA TGATGGATAC TTTCTCGGCA 
     6901 GGAGCAAGGT GAGATGACAG GAGATCCTGC CCCGGCACTT CGCCCAATAG CAGCCAGTCC 
     6961 CTTCCCGCTT CAGTGACAAC GTCGAGCACA GCTGCGCAAG GAACGCCCGT CGTGGCCAGC 
     7021 CACGATAGCC GCGCTGCCTC GTCTTGCAGT TCATTCAGGG CACCGGACAG GTCGGTCTTG 
     7081 ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC AGCCGGAACA CGGCGGCATC AGAGCAGCCG 
     7141 ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT AGCCTCTCCA CCCAAGCGGC CGGAGAACCT 
     7201 GCGTGCAATC CATCTTGTTC AATCATGCGA AACGATCCTC ATCCTGTCTC TTGATCGATC 
     7261 TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC TCCTCACTAC TTCTGGAATA GCTCAGAGGC 
     7321 CGAGGCGGCC TCGGCCTCTG CATAAATAAA AAAAATTAGT CAGCCATGGG GCGGAGAATG 
     7381 GGCGGAACTG GGCGGAGTTA GGGGCGGGAT GGGCGGAGTT AGGGGCGGGA CTATGGTTGC 
     7441 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     7501 ACACCTGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     7561 TGGGGACTTT CCACACCCTA ACTGACACAC ATTCCACAGC TGGTTCTTTC CGCCTCAGGA 
     7621 CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC 
     7681 ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA 
     7741 GTGCCACCTG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC 
     7801 AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC 
     7861 TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG 
     7921 TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA 
     7981 CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC 
     8041 TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT 
     8101 TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA 
     8161 CAAAAATTTA ACGCGAATTT T
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