LOCUS       MR211619                8157 bp    DNA     circular  SYN 
DEFINITION  Esyt1 (Myc-DDK-tagged) - Mouse extended synaptotagmin-like protein 1 (Esyt1)
ACCESSION   NM_011843
VERSION     NM_011843.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4314..4405 
                     /label="Myc-DDK"  
     misc_feature    4435..5020 
                     /label="PolyA signal"  
     misc_feature    4492..4511 
                     /label="Restriction Enzyme"  
     misc_feature    5296..5968 
                     /label="ColE1"  
     misc_feature    6398..7192 
                     /label="Kan/Neo"  
     misc_feature    7227..7529 
                     /label="SV40 ori"  
     misc_feature    7615..8092 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4296..4302 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4296 
                     /label="Esyt1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGCACTCC CCTGAAGAGG GCGCCAGCCC CGAGCCGTCA GGGCAGCCCC 
     1081 CTGCCACGGA CTCCACGCGG GACGGGGGCT CCGGGGTCCC ACCTGCCGGC CCAGGTGCGG 
     1141 CGAGCGAGGC CCTGGCGGTG CTGACTTCCT TCGGGCGCCG CTTGTTGGTG CTGGTGCCGG 
     1201 TGTACCTGGC AGGGGCAGCG GGTCTTAGCG TAGGTTTCGT GCTTTTCGGC CTCGCCCTGT 
     1261 ACTTGGGCTG GCGCCGGGTC CGCGATGGGA AAGAACGGAG CCTGAGGGCA GCGAGGCAGC 
     1321 TGCTGGATGA CGAGGAGCGG ATCACCGCAG AGACGCTTTA TATGAGCCAC CGGGAACTAC 
     1381 CTGCCTGGGT CAGCTTCCCA GATGTGGAAA AGGCCGAATG GCTGAACAAG ATCGTGGCTC 
     1441 AGGTGTGGCC CTTCCTAGGC CAGTATATGG AGAAGCTTCT GGCGGAGACA GTGGCCCCAG 
     1501 CTGTCCGGGG AGCTAACCCT CATCTGCAGA CATTCACATT CACACGTGTG GAGCTGGGTG 
     1561 AAAAGCCATT ACGAATCATT GGCGTCAAAG TTCACCCTAG TCAGAGGAAA GATCAGATTC 
     1621 TGTTGGACTT GAATGTCAGC TATGTAGGTG ATGTACAGAT TGACGTGGAG GTGAAGAAAT 
     1681 ATTTCTGCAA AGCTGGAGTC AAGGGCATGC AGCTCCATGG TGTCTTGCGA GTGATTCTTG 
     1741 AGCCACTCAC AGGGGACCTT CCTATCGTGG GGGCTGTGTC CATGTTCTTT ATCAAACGCC 
     1801 CGACGCTTGA CATCAACTGG ACAGGGATGA CCAACCTGCT AGATATCCCA GGACTTAGCT 
     1861 CACTCTCTGA CACCATGATC ATGGACTCCA TTGCTGCCTT CCTCGTGCTC CCTAACCGAC 
     1921 TGTTGGTGCC CCTTGTGCCC GACCTTCAAG ATGTGGCCCA GCTGCGGTCC CCACTACCCA 
     1981 GGGGCATTAT CCGGATTCAC CTGCTGGCAG CCCGAGGTCT GAGTTCCAAG GACAAGTATG 
     2041 TGAAAGGCCT GATTGAGGGC AAATCGGATC CCTACGCGCT CGTCCGTGTG GGCACCCAGA 
     2101 CGTTCTGCAG CCGTGTCATA GATGAGGAGC TCAACCCTCA CTGGGGAGAG ACATATGAGG 
     2161 TGATAGTCCA CGAGGTTCCA GGACAGGAGA TCGAGGTGGA GGTATTTGAC AAAGATCCAG 
     2221 ATAAAGATGA TTTTCTGGGA AGAATGAAGC TGGACGTGGG GAAGGTATTA CAGGCTGGAG 
     2281 TCCTGGATAA TTGGTACCCT CTGCAAGGCG GGCAAGGCCA AGTTCACTTG AGACTAGAAT 
     2341 GGCTATCACT CCTGCCAGAT GCAGAGAAGC TGGATCAGGT CCTGCAGTGG AATCGGGGCA 
     2401 TCACTTCTCG GCCAGAGCCC CCATCGGCTG CCATCCTCGT TGTCTACTTG GACCGAGCCC 
     2461 AGGACCTTCC TCTGAAGAAA GGCAACAAGG AACCCAACCC CATGGTGCAA CTATCAGTTC 
     2521 AGGATGTGAC CCGGGAGAGC AAGGCTACCT ACAGCACCAA CAGCCCGGTG TGGGAAGAGG 
     2581 CTTTCCGGTT TTTCCTGCAG GACCCTCGAA GCCAGGAGCT TGATGTACAG GTGAAGGACG 
     2641 ACTCCCGAGC CCTGACTTTA GGGGCCCTGA CTCTCCCTCT TGCGCGCCTG TTGACTGCCT 
     2701 CTGAACTCAC CCTGGACCAG TGGTTCCAGC TCAGCAGCTC AGGCCCAAAC TCCAGGCTCT 
     2761 ACATGAAACT GGTCATGCGG ATCTTATACT TAGATTACTC GGAAATCCGC TTCCCCACTG 
     2821 TGCCTGGTGC CCAGGACTGG GACCGTGAGA GCCTAGAGAC AGGCAGCAGT GTGGATGCCC 
     2881 CACCTCGGCC CTATCACACA ACCCCTAACA GCCACTTTGG GACTGAGAAT GTTCTTCGGA 
     2941 TCCATGTATT AGAAGCGCAG GACCTAATTG CCAAAGACCG TTTCTTGGGA GGCCTGGTGA 
     3001 AGGGCAAATC AGACCCCTAC GTCAAACTAA AGGTGGCAGG AAAAAGTTTC CGGACCCATG 
     3061 TTGTTCGGGA AGATCTCAAT CCCCGCTGGA ATGAGGTTTT TGAGGTGATT GTCACATCAA 
     3121 TCCCTGGCCA AGAGCTTGAG ATTGAGGTCT TTGATAAGGA CTTGGACAAG GATGACTTTT 
     3181 TGGGCAGGTA TAAAGTGAGT CTCACCACAG TGCTCAACAG TGGCTTTCTT GATGAGTGGC 
     3241 TGACCCTGGA GGATGTCCCA TCCGGGCGCC TGCACTTGCG CCTGGAGCGT CTGACCCCTA 
     3301 GACCGACTGC TGCTGAGTTA GAGGAGGTGC TGCAGGTGAA CAGCCTGATC CAGACTCAGA 
     3361 AGAGTTCAGA GCTGGCAGCA GCCCTGCTGT CCGTCTTCCT GGAGCGGGCA GAAGATCTGC 
     3421 CGCTCCGAAA AGGTACCAAG CCTCCCAGCC CTTACGCTAC TATCACCGTG GGAGAGACTT 
     3481 CCCATAAAAC CAAGACTGTC TCCCAATCTT CGGCCCCTGT CTGGGAGGAG AGTGCCTCTT 
     3541 TTCTCATCAG GAAGCCACAC GCTGAGAGCC TGGAGCTGCA GGTTCGGGGT GAAGGGACCG 
     3601 GCACCCTGGG CTCTGTATCC CTTCCTCTCT CTGAGCTCCT CCAGGAGGAC CAGCTCTGCC 
     3661 TGGACCACTG GTTTGCACTC AGTGGTCAGG GCCAGGTGCT GATGAGAGCG CAGCTTGGGA 
     3721 TCCTGGTATC TCAGCACTCG GGAGTGGAAG CCCACAGCCA CAGCTACAGC CACAGTCACA 
     3781 GCTCCTCCTC GCTCAATGAC GAACCTGAGG CCTTAGGGGG ACCCACTCAC CCTGCCTCCC 
     3841 CGGTCCTAGA GGTCAGGCAT CGCCTAACAC ATGGTGACAG TCCCTCTGAG GCTCCCGTTG 
     3901 GGCCCCTGGG CCAGGTGAAG CTGACAGTGT GGTACCACAG CGACGAACAA AAGCTGATCA 
     3961 GCATTATTCA CAGCTGCCGG GCCCTTCGAC AAAATGGACG TGATCTCCCA GACCCCTATG 
     4021 TGTCAGTGTT GCTGCTCCCA GACAAGAACC GAAGCACCAA GAGGAAGACC CCGCAGAAGA 
     4081 AGAGGACCCT CAATCCTGAG TTCAATGAGC GGTTTGAGTG GGATCTGCCC CTGGATGGGA 
     4141 CTCTCAGGCG GAAGCTCGAT GTCTCTGTGA AGTCAAACTC CTCCTTCATG TCACGAGAGC 
     4201 GTGAGCTTCT GGGGAAGGTG CAGCTGGATC TAGCAGAGAT AGACCTTTCC CAGGGTGCAG 
     4261 CCCAGTGGTA TGACCTGATG GATGACAGAG ATAAGGGCGG TTCCACGCGT ACGCGGCCGC 
     4321 TCGAGCAGAA ACTCATCTCA GAAGAGGATC TGGCAGCAAA TGATATCCTG GATTACAAGG 
     4381 ATGACGACGA TAAGGTTTAA ACGGCCGGCC GCGGTCATAG CTGTTTCCTG AACAGATCCC 
     4441 GGGTGGCATC CCTGTGACCC CTCCCCAGTG CCTCTCCTGG CCCTGGAAGT TGCCACTCCA 
     4501 GTGCCCACCA GCCTTGTCCT AATAAAATTA AGTTGCATCA TTTTGTCTGA CTAGGTGTCC 
     4561 TTCTATAATA TTATGGGGTG GAGGGGGGTG GTATGGAGCA AGGGGCAAGT TGGGAAGACA 
     4621 ACCTGTAGGG CCTGCGGGGT CTATTGGGAA CCAAGCTGGA GTGCAGTGGC ACAATCTTGG 
     4681 CTCACTGCAA TCTCCGCCTC CTGGGTTCAA GCGATTCTCC TGCCTCAGCC TCCCGAGTTG 
     4741 TTGGGATTCC AGGCATGCAT GACCAGGCTC AGCTAATTTT TGTTTTTTTG GTAGAGACGG 
     4801 GGTTTCACCA TATTGGCCAG GCTGGTCTCC AACTCCTAAT CTCAGGTGAT CTACCCACCT 
     4861 TGGCCTCCCA AATTGCTGGG ATTACAGGCG TGAACCACTG CTCCCTTCCC TGTCCTTCTG 
     4921 ATTTTAAAAT AACTATACCA GCAGGAGGAC GTCCAGACAC AGCATAGGCT ACCTGGCCAT 
     4981 GCCCAACCGG TGGGACATTT GAGTTGCTTG CTTGGCACTG TCCTCTCATG CGTTGGGTCC 
     5041 ACTCAGTAGA TGCCTGTTGA ATTGGGTACG CGGCCAGCGG CGAGCGGTAT CAGCTCACTC 
     5101 AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC 
     5161 AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG 
     5221 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC 
     5281 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT 
     5341 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT 
     5401 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG 
     5461 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT 
     5521 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT 
     5581 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG 
     5641 CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA 
     5701 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT 
     5761 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC 
     5821 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT 
     5881 ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA 
     5941 AAGTATATAT GAGTAACCTG AGGCTATGGC AGGGCCTGCC GCCCCGACGT TGGCTGCGAG 
     6001 CCCTGGGCCT TCACCCGAAC TTGGGGGGTG GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA 
     6061 TTGGCCCCAA TGGGGTCTCG GTGGGGTATC GACAGAGTGC CAGCCCTGGG ACCGAACCCC 
     6121 GCGTTTATGA ACAAACGACC CAACACCGTG CGTTTTATTC TGTCTTTTTA TTGCCGTCAT 
     6181 AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG 
     6241 GGCGAAGAAC TCCAGCATGA GATCCCCGCG CTGGAGGATC ATCCAGCCGG CGTCCCGGAA 
     6301 AACGATTCCG AAGCCCAACC TTTCATAGAA GGCGGCGGTG GAATCGAAAT CTCGTGATGG 
     6361 CAGGTTGGGC GTCGCTTGGT CGGTCATTTC GAACCCCAGA GTCCCGCTCA GAAGAACTCG 
     6421 TCAAGAAGGC GATAGAAGGC GATGCGCTGC GAATCGGGAG CGGCGATACC GTAAAGCACG 
     6481 AGGAAGCGGT CAGCCCATTC GCCGCCAAGC TCTTCAGCAA TATCACGGGT AGCCAACGCT 
     6541 ATGTCCTGAT AGCGATCCGC CACACCCAGC CGGCCACAGT CGATGAATCC AGAAAAGCGG 
     6601 CCATTTTCCA CCATGATATT CGGCAAGCAG GCATCGCCAT GGGTCACGAC GAGATCCTCG 
     6661 CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG AACAGTTCGG CTGGCGCGAG CCCCTGATGC 
     6721 TCTTCGTCCA GATCATCCTG ATCGACAAGA CCGGCTTCCA TCCGAGTACG TGCTCGCTCG 
     6781 ATGCGATGTT TCGCTTGGTG GTCGAATGGG CAGGTAGCCG GATCAAGCGT ATGCAGCCGC 
     6841 CGCATTGCAT CAGCCATGAT GGATACTTTC TCGGCAGGAG CAAGGTGAGA TGACAGGAGA 
     6901 TCCTGCCCCG GCACTTCGCC CAATAGCAGC CAGTCCCTTC CCGCTTCAGT GACAACGTCG 
     6961 AGCACAGCTG CGCAAGGAAC GCCCGTCGTG GCCAGCCACG ATAGCCGCGC TGCCTCGTCT 
     7021 TGCAGTTCAT TCAGGGCACC GGACAGGTCG GTCTTGACAA AAAGAACCGG GCGCCCCTGC 
     7081 GCTGACAGCC GGAACACGGC GGCATCAGAG CAGCCGATTG TCTGTTGTGC CCAGTCATAG 
     7141 CCGAATAGCC TCTCCACCCA AGCGGCCGGA GAACCTGCGT GCAATCCATC TTGTTCAATC 
     7201 ATGCGAAACG ATCCTCATCC TGTCTCTTGA TCGATCTTTG CAAAAGCCTA GGCCTCCAAA 
     7261 AAAGCCTCCT CACTACTTCT GGAATAGCTC AGAGGCCGAG GCGGCCTCGG CCTCTGCATA 
     7321 AATAAAAAAA ATTAGTCAGC CATGGGGCGG AGAATGGGCG GAACTGGGCG GAGTTAGGGG 
     7381 CGGGATGGGC GGAGTTAGGG GCGGGACTAT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     7441 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CTGGTTGCTG ACTAATTGAG 
     7501 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCCTAACTG 
     7561 ACACACATTC CACAGCTGGT TCTTTCCGCC TCAGGACTCT TCCTTTTTCA ATATTATTGA 
     7621 AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT 
     7681 AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGC GCCCTGTAGC 
     7741 GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC 
     7801 GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT 
     7861 CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC 
     7921 CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG 
     7981 ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA 
     8041 ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG 
     8101 ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTT
//