LOCUS       MR211618                8154 bp    DNA     circular  SYN 
DEFINITION  Acly (Myc-DDK-tagged) - Mouse ATP citrate lyase (Acly), transcript variant 2
ACCESSION   NM_134037
VERSION     NM_134037.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4311..4402 
                     /label="Myc-DDK"  
     misc_feature    4432..5017 
                     /label="PolyA signal"  
     misc_feature    4489..4508 
                     /label="Restriction Enzyme"  
     misc_feature    5293..5965 
                     /label="ColE1"  
     misc_feature    6395..7189 
                     /label="Kan/Neo"  
     misc_feature    7224..7526 
                     /label="SV40 ori"  
     misc_feature    7612..8089 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4293..4299 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4293 
                     /label="Acly"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCAGCCAAG GCAATTTCAG AGCAGACCGG CAAAGAACTC CTGTACAAGT 
     1081 ACATCTGCAC CACCTCGGCC ATCCAGAACC GGTTCAAGTA CGCCCGGGTG ACTCCCGACA 
     1141 CAGACTGGGC CCATCTGCTG CAGGACCACC CGTGGCTGCT CAGCCAGAGC TTGGTTGTCA 
     1201 AGCCAGACCA GTTAATCAAA CGTCGAGGAA AGCTTGGCCT CGTCGGGGTC AATCTCTCTC 
     1261 TGGATGGAGT CAAATCCTGG CTAAAACCTC GCCTGGGACA TGAAGCCACT GTCGGCAAGG 
     1321 CCAAAGGCTT CCTCAAGAAC TTTCTCATTG AACCCTTCGT CCCCCACAGT CAGGCGGAGG 
     1381 AGTTCTACGT GTGCATCTAT GCTACCCGGG AAGGAGACTA CGTCCTGTTC CACCATGAAG 
     1441 GGGGTGTGGA TGTGGGCGAT GTGGATGCCA AAGCCCAGAA GCTGCTTGTG GGTGTGGACG 
     1501 AAAAGCTGAA TACCGAGGAC ATTAAGAGAC ACCTGTTGGT CCATGCACCT GAGGACAAGA 
     1561 AAGAAGTCCT GGCCAGCTTC ATCTCTGGTC TATTCAATTT CTACGAGGAT CTGTACTTCA 
     1621 CCTACCTTGA GATCAACCCC CTTGTGGTGA CCAAAGATGG TGTCTACATC CTTGACTTGG 
     1681 CGGCCAAGGT GGATGCCACA GCTGACTACA TCTGTAAAGT CAAGTGGGGT GATATAGAGT 
     1741 TCCCTCCCCC CTTTGGGCGT GAGGCGTACC CCGAGGAAGC CTACATTGCA GACCTGGATG 
     1801 CCAAAAGTGG AGCAAGCTTG AAGCTGACCT TGCTGAACCC CAAGGGGCGG ATCTGGACCA 
     1861 TGGTTGCTGG GGGTGGCGCC TCCGTCGTGT ACAGTGACAC CATCTGTGAT CTTGGAGGTG 
     1921 TCAATGAACT GGCGAATTAC GGGGAATACT CGGGTGCCCC CAGTGAACAA CAGACCTATG 
     1981 ACTATGCCAA GACCATCCTC TCACTTATGA CTCGAGAGAA GCACCCAGAA GGCAAGATCC 
     2041 TCATCATTGG AGGCAGCATT GCAAACTTCA CCAATGTGGC CGCCACCTTC AAGGGCATTG 
     2101 TGAGAGCGAT TCGAGATTAC CAGGGTCCCC TGAAGGAGCA TGAGGTCACC ATCTTTGTCC 
     2161 GAAGAGGTGG CCCCAACTAT CAAGAGGGAT TACGAGTGAT GGGAGAAGTT GGGAAGACCA 
     2221 CTGGGATCCC CATCCATGTC TTTGGCACAG AAACTCACAT GACGGCCATT GTGGGCATGG 
     2281 CCCTGGGCCA CCGGCCCATT CCCAACCAGC CACCCACAGC AGCTCACACT GCCAACTTCC 
     2341 TCCTTAATGC CAGCGGGAGC ACATCGACTC CAGCACCCAG TAGGACAGCA TCTTTTTCTG 
     2401 AGTCCCGAGC TGATGAAGTG GCACCTGCAA AGAAAGCCAA GCCAGCTATG CCCCAAGGAA 
     2461 AGAGTGCCAC CCTCTTCAGC CGACATACCA AGGCCATCGT GTGGGGCATG CAGACCCGGG 
     2521 CTGTGCAGGG CATGCTGGAC TTTGACTACG TGTGTTCCCG AGACGAGCCC TCAGTGGCTG 
     2581 CCATGGTCTA CCCTTTCACT GGGGATCACA AGCAGAAGTT TTACTGGGGA CACAAGGAAA 
     2641 TCCTGATCCC TGTCTTCAAG AACATGGCTG ACGCCATGAA GAAGCACCCG GAGGTAGACG 
     2701 TGCTGATCAA CTTTGCGTCT CTGCGGTCCG CTTACGACAG CACCATGGAG ACCATGAACT 
     2761 ATGCCCAGAT CCGCACCATA GCCATCATAG CAGAAGGTAT CCCTGAGGCC CTCACACGGA 
     2821 AGCTCATCAA GAAGGCCGAC CAGAAGGGAG TGACCATCAT TGGGCCAGCT ACGGTTGGGG 
     2881 GCATTAAGCC TGGATGCTTT AAGATCGGGA ATACTGGTGG AATGCTGGAC AACATCCTGG 
     2941 CCTCCAAACT GTACCGCCCA GGCAGCGTGG CCTACGTCTC ACGTTCAGGA GGCATGTCTA 
     3001 ATGAACTCAA TAACATCATC TCTCGGACCA CAGATGGTGT CTATGAGGGC GTGGCCATCG 
     3061 GCGGGGACAG GTACCCTGGG TCCACATTCA TGGATCACGT GCTGCGCTAC CAGGACACTC 
     3121 CAGGAGTCAA GATGATCGTA GTTCTTGGGG AGATAGGGGG CACAGAGGAA TATAAGATCT 
     3181 GCCGGGGCAT CAAGGAGGGC CGCCTCACCA AGCCAGTGGT CTGCTGGTGT ATCGGGACCT 
     3241 GTGCCACCAT GTTCTCCTCC GAGGTCCAGT TTGGCCATGC TGGAGCTTGT GCCAACCAGG 
     3301 CTTCTGAAAC TGCAGTAGCC AAGAACCAGG CCTTGAAGGA AGCAGGAGTG TTTGTGCCCC 
     3361 GAAGCTTCGA TGAGCTTGGA GAAATCATTC AGTCTGTGTA TGAAGATCTG GTGGCCAAAG 
     3421 GAGCCATTGT ACCTGCCCAG GAAGTGCCAC CTCCAACAGT GCCCATGGAC TACTCTTGGG 
     3481 CCAGAGAGCT GGGTTTGATC CGAAAACCTG CCTCATTCAT GACCAGCATC TGTGATGAGC 
     3541 GAGGGCAGGA GCTCATTTAT GCGGGCATGC CCATCACCGA GGTCTTCAAG GAGGAGATGG 
     3601 GCATCGGTGG TGTCCTCGGC CTCCTCTGGT TCCAGAGAAG GTTGCCCAAG TATTCCTGCC 
     3661 AGTTCATTGA GATGTGTCTG ATGGTCACAG CTGATCACGG GCCAGCTGTC TCTGGAGCCC 
     3721 ATAACACCAT CATCTGTGCT CGGGCTGGGA AGGACCTGGT CTCCAGCCTC ACCTCAGGGC 
     3781 TGCTCACCAT TGGAGACCGG TTTGGGGGTG CCTTGGATGC CGCAGCAAAG ATGTTCAGTA 
     3841 AAGCCTTTGA CAGCGGCATC ATTCCCATGG AGTTCGTCAA CAAGATGAAG AAGGAGGGGA 
     3901 AGCTGATCAT GGGCATCGGC CATCGAGTAA AATCGATAAA CAACCCAGAC ATGCGAGTGC 
     3961 AGATCCTCAA GGACTTCGTC AAACAGCACT TCCCCGCCAC CCCGCTGCTC GACTATGCCC 
     4021 TGGAAGTGGA GAAGATTACC ACCTCCAAGA AGCCAAATCT TATCCTGAAT GTGGACGGCT 
     4081 TCATCGGCGT TGCGTTTGTG GACATGCTCA GGAACTGTGG CTCCTTCACC CGGGAGGAAG 
     4141 CTGATGAATA TGTTGACATT GGAGCCCTCA ATGGCATCTT TGTGCTAGGA AGGAGTATGG 
     4201 GCTTCATTGG GCACTACCTT GACCAGAAGA GGCTGAAGCA AGGGCTGTAT CGTCACCCCT 
     4261 GGGATGACAT TTCCTATGTT CTTCCAGAAC ACATGAGCAT GACGCGTACG CGGCCGCTCG 
     4321 AGCAGAAACT CATCTCAGAA GAGGATCTGG CAGCAAATGA TATCCTGGAT TACAAGGATG 
     4381 ACGACGATAA GGTTTAAACG GCCGGCCGCG GTCATAGCTG TTTCCTGAAC AGATCCCGGG 
     4441 TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG 
     4501 CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC 
     4561 TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC 
     4621 TGTAGGGCCT GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC 
     4681 ACTGCAATCT CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG 
     4741 GGATTCCAGG CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT 
     4801 TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG 
     4861 CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT 
     4921 TTAAAATAAC TATACCAGCA GGAGGACGTC CAGACACAGC ATAGGCTACC TGGCCATGCC 
     4981 CAACCGGTGG GACATTTGAG TTGCTTGCTT GGCACTGTCC TCTCATGCGT TGGGTCCACT 
     5041 CAGTAGATGC CTGTTGAATT GGGTACGCGG CCAGCGGCGA GCGGTATCAG CTCACTCAAA 
     5101 GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA 
     5161 AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT 
     5221 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 
     5281 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 
     5341 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 
     5401 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 
     5461 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 
     5521 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 
     5581 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 
     5641 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 
     5701 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 
     5761 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC 
     5821 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC 
     5881 AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG 
     5941 TATATATGAG TAACCTGAGG CTATGGCAGG GCCTGCCGCC CCGACGTTGG CTGCGAGCCC 
     6001 TGGGCCTTCA CCCGAACTTG GGGGGTGGGG TGGGGAAAAG GAAGAAACGC GGGCGTATTG 
     6061 GCCCCAATGG GGTCTCGGTG GGGTATCGAC AGAGTGCCAG CCCTGGGACC GAACCCCGCG 
     6121 TTTATGAACA AACGACCCAA CACCGTGCGT TTTATTCTGT CTTTTTATTG CCGTCATAGC 
     6181 GCGGGTTCCT TCCGGTATTG TCTCCTTCCG TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC 
     6241 GAAGAACTCC AGCATGAGAT CCCCGCGCTG GAGGATCATC CAGCCGGCGT CCCGGAAAAC 
     6301 GATTCCGAAG CCCAACCTTT CATAGAAGGC GGCGGTGGAA TCGAAATCTC GTGATGGCAG 
     6361 GTTGGGCGTC GCTTGGTCGG TCATTTCGAA CCCCAGAGTC CCGCTCAGAA GAACTCGTCA 
     6421 AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA TCGGGAGCGG CGATACCGTA AAGCACGAGG 
     6481 AAGCGGTCAG CCCATTCGCC GCCAAGCTCT TCAGCAATAT CACGGGTAGC CAACGCTATG 
     6541 TCCTGATAGC GATCCGCCAC ACCCAGCCGG CCACAGTCGA TGAATCCAGA AAAGCGGCCA 
     6601 TTTTCCACCA TGATATTCGG CAAGCAGGCA TCGCCATGGG TCACGACGAG ATCCTCGCCG 
     6661 TCGGGCATGC TCGCCTTGAG CCTGGCGAAC AGTTCGGCTG GCGCGAGCCC CTGATGCTCT 
     6721 TCGTCCAGAT CATCCTGATC GACAAGACCG GCTTCCATCC GAGTACGTGC TCGCTCGATG 
     6781 CGATGTTTCG CTTGGTGGTC GAATGGGCAG GTAGCCGGAT CAAGCGTATG CAGCCGCCGC 
     6841 ATTGCATCAG CCATGATGGA TACTTTCTCG GCAGGAGCAA GGTGAGATGA CAGGAGATCC 
     6901 TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG TCCCTTCCCG CTTCAGTGAC AACGTCGAGC 
     6961 ACAGCTGCGC AAGGAACGCC CGTCGTGGCC AGCCACGATA GCCGCGCTGC CTCGTCTTGC 
     7021 AGTTCATTCA GGGCACCGGA CAGGTCGGTC TTGACAAAAA GAACCGGGCG CCCCTGCGCT 
     7081 GACAGCCGGA ACACGGCGGC ATCAGAGCAG CCGATTGTCT GTTGTGCCCA GTCATAGCCG 
     7141 AATAGCCTCT CCACCCAAGC GGCCGGAGAA CCTGCGTGCA ATCCATCTTG TTCAATCATG 
     7201 CGAAACGATC CTCATCCTGT CTCTTGATCG ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA 
     7261 GCCTCCTCAC TACTTCTGGA ATAGCTCAGA GGCCGAGGCG GCCTCGGCCT CTGCATAAAT 
     7321 AAAAAAAATT AGTCAGCCAT GGGGCGGAGA ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG 
     7381 GATGGGCGGA GTTAGGGGCG GGACTATGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     7441 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCTG GTTGCTGACT AATTGAGATG 
     7501 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC CTAACTGACA 
     7561 CACATTCCAC AGCTGGTTCT TTCCGCCTCA GGACTCTTCC TTTTTCAATA TTATTGAAGC 
     7621 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA 
     7681 CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC 
     7741 GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC 
     7801 CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC 
     7861 CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC 
     7921 GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG 
     7981 GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT 
     8041 GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT 
     8101 TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
//