LOCUS       MR211601                8121 bp    DNA     circular  SYN 
DEFINITION  Usp8 (Myc-DDK-tagged) - Mouse ubiquitin specific peptidase 8 (Usp8)
ACCESSION   NM_019729
VERSION     NM_019729.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4278..4369 
                     /label="Myc-DDK"  
     misc_feature    4399..4984 
                     /label="PolyA signal"  
     misc_feature    4456..4475 
                     /label="Restriction Enzyme"  
     misc_feature    5260..5932 
                     /label="ColE1"  
     misc_feature    6362..7156 
                     /label="Kan/Neo"  
     misc_feature    7191..7493 
                     /label="SV40 ori"  
     misc_feature    7579..8056 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4260..4266 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4260 
                     /label="Usp8"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCTGCTGTA GCTTCAGTTC CTAAAGAACT CTACCTCAGT TCTTCACTAA 
     1081 AAGACCTCAA TAAGAAGACC GAAGTTAAAC CTGAGAAAAC CAGCACCAAG AATTATATAC 
     1141 ACAGCGCGCA GAAGATCTTC AAGACAGCAG AAGAATGCAG ACTAGATCGT GATGAGGAAA 
     1201 GGGCCTATGT GCTTTATATG AAATATGTGG CAGTTTATAA TCTTATCAAA AAGAGACCTG 
     1261 ATTTCAAGCA ACAGCAGGAT TATTATCTTT CAATACTTGG ACCTGCAAAC ATCAAAAAAG 
     1321 CTATTGAAGA AGCTGAAAGA CTCTCCGAAA GCCTTAAACT AAGATACGAA GAGGCTGAAG 
     1381 TTCGGAAACA ACTTGAAGAA AAGGACAGAC GGGAGGAAGA GCAGCTGCAG CAACAGAAAA 
     1441 GGCAGGAGAT GGGGAGAGAG GACAGCGGTG CGGCAGCCAA ACGCTCTGTG GAAAATTTAC 
     1501 TGGATTCCAA AACCAAAACC CAAAGGATTA ACGGCGAGAA GAGTGAAGGA GCTGCAGCTG 
     1561 CAGAGAGAGG AGCCATCACA GCAAAGGAAC TATATACGAT GATGATGGAT AAAAACACAA 
     1621 GCTTAATTAT AATGGATGCT CGAAAAATAC AGGATTATCA GCATTCCTGT ATCTTGGATT 
     1681 CTCTCAGTGT TCCTGAAGAA GCTATCAGTC CAGGAGTCAC TGCTAGTTGG ATTGAAGCAA 
     1741 ACCTCTCAGA TGATTCTAAA GACACATGGA AAAAGAGGGG GAGTGTGGAC TATGTGGTCC 
     1801 TTCTCGACTG GTTTAGTTCA GCGAAAGATT TGCTGCTTGG GACCACTCTA CGGAGTCTGA 
     1861 AAGATGCACT TTTCAAGTGG GAAAGTAAAA CTGTCCTGCG CCATGAGCCT TTGGTGTTGG 
     1921 AGGGCGGCTA TGAAAACTGG CTGCTTTGCT ACCCGCAGTT TACAACCAAT GCTAAGGTCA 
     1981 CTCCACCCCC TCGGAGCAGA GCTGAAGAGG TGTCTGTCTC ATTGGATTTT ACTTATCCCT 
     2041 CATTGGAAGA ACCAGTTCCT TCCAAACTTC CTACCCAGAT GCCACCTCCT CCTATAGAAA 
     2101 CAAATGAAAA GGCACTGTTG GTAACTGATC AAGATGAAAA GCTGAGACTG TCAACCCAGC 
     2161 CAGCTCTAGC TGGACCTGGT GCGGCTCCCA GAGCTGAAGC CTCACCCATA ATTCAGCCAG 
     2221 CGCCTGCTAC AAAGAGTGTT CCACAGGTTG ATCGTACGAA AAAACCGTCA GTCAAGTTGC 
     2281 CTGAAGATCA TAGAATAAAA TCTGAAAATA CAGATCAGAG TGGAAGAGTT CTTTCTGATC 
     2341 GATCCACCAA GCCAGTATTT CCCTCTCCAA CCACCATGTT AACAGATGAA GAAAAGGCTC 
     2401 GTATTCATCA AGAAACTGCC CTTCTTATGG AAAAGAATAA ACAGGAGAAG GAACTTTGGG 
     2461 ACAAGCAGCA GAAGGAACAG AAAGAGAAGC TGAGAAGGGA GGAACAAGAG CGCAAAGCTG 
     2521 GAAAGACACA GGATGCAGAT GAACGTGACT CCACTGAGAA TCAGCACAAA GCAAAGGATG 
     2581 GACAAGAGAA AAAAGACAGC AAACAGACCA AGACGGAAGA CAGAGAGCTT TCAGCAGACG 
     2641 GGGCCCAGGA AGCCACAGGA ACACAAAGAC AAAGTAAGAG TGAGCATGAA GCTTCTGATG 
     2701 CTAAGGTACC TGTGGAAGGT AAAAGGTGTC CCACGTCAGA GGCGCAGAAG AGGCCAGCAG 
     2761 ATGTGTCCCC TGCATCCGTG TCAGGAGAGC TGAATGCAGG CAAGGCTCAG CGAGAACCTT 
     2821 TGACGAGAGC ACGAAGTGAA GAAATGGGGA GAATTGTGCC GGGACTGCCT TTGGGCTGGG 
     2881 CCAAGTTTCT TGATCCAATC ACCGGGACCT TTCGTTACTA CCATTCCCCC ACAAACACAG 
     2941 TTCATATGTA TCCACCTGAA ATGGCTCCTT CGTCTGCACC TCCTTCCACC CCGCCCACTC 
     3001 ACAAAGTCAA GCCCCAGGTC CCTGCCGAGC GGGACAGGGA GCCATCGAAA CTGAAGCGCT 
     3061 CCTACTCCTC ACCAGATATC ACTCAGGCCC TGCAGGAGGA GGAGAAGAGG AGGCCGGCAG 
     3121 TGACCCCGAT GGTCAACCGG GAAAACAAGC CACCATGTTA CCCTAAAGCT GAGATTTCGA 
     3181 GGCTTTCTGC TTCTCAGATT CGGAACCTCA ATCCTGTATT TGGAGGATCA GGACCAGCTC 
     3241 TTACTGGACT TCGTAATTTG GGAAATACTT GTTACATGAA CTCAATCTTG CAGTGCCTGT 
     3301 GTAATGCTCC ACATCTGGCT GATTATTTCA ACCGAAACTG CTACCAGGAT GATATCAACA 
     3361 GGTCAAATTT GTTGGGGCAT AAAGGTGAAG TGGCAGAAGA ATTTGGTATA ATCATGAAAG 
     3421 CACTGTGGAC AGGACAGTAT AGATACATCA GTCCAAAGGA CTTTAAAGTC ACCATTGGTA 
     3481 AGATTAATGA CCAGTTTGCA GGCTCCAGCC AACAGGATTC ACAAGAGCTG CTTCTGTTCC 
     3541 TCATGGATGG CCTGCATGAG GATCTGAATA AGGCTGACAA TCGGAAGAGG CACAAGGAAG 
     3601 AGAACAACGA GCACCTGGAT GACCTGCAGG CGGCCGAGCA CGCCTGGCAG AAGCACAAGC 
     3661 AGCTCAACGA GTCCATCATC GTGGCCCTGT TCCAGGGCCA GTTCAAGTCC ACAGTGCAGT 
     3721 GCCTCACCTG CCGCAGGAGG TCGCGCACCT TCGAGGCCTT CATGTACTTG TCTTTGCCGC 
     3781 TAGCATCCAC AAGTAAATGT ACTCTACAGG ACTGCCTTAG ATTATTTTCC AAAGAAGAAA 
     3841 AGCTTACAGA TAACAACAGA TTTTACTGCA GCCATTGCCG AGCTCGGCGG GATTCTCTAA 
     3901 AGAAAATAGA AATCTGGAAA TTACCTCCTG TGCTGTTAGT GCACCTGAAA CGATTTTCCT 
     3961 ATGACGGCAG GTGGAAGCAG AAGCTGCAAA CATCCGTGGA TTTCCCATTG GAAAATCTTG 
     4021 ACCTGTCACA GTACGTTATT GGTCCAAAAA ACAGCTTGAA GAAATATAAC TTGTTTTCTG 
     4081 TTTCAAACCA CTACGGCGGG CTAGACGGAG GCCACTACAC AGCCTACTGT AAGAACGCGG 
     4141 CAAGGCAGCG CTGGTTTAAG TTTGATGACC ATGAAGTTTC TGATATCTCT GTGTCTTCTG 
     4201 TGAGGTCATC AGCAGCTTAT ATCCTCTTTT ATACTTCCCT GGGACCACGC ATAACTGATG 
     4261 TAGCCACAAC GCGTACGCGG CCGCTCGAGC AGAAACTCAT CTCAGAAGAG GATCTGGCAG 
     4321 CAAATGATAT CCTGGATTAC AAGGATGACG ACGATAAGGT TTAAACGGCC GGCCGCGGTC 
     4381 ATAGCTGTTT CCTGAACAGA TCCCGGGTGG CATCCCTGTG ACCCCTCCCC AGTGCCTCTC 
     4441 CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC ACCAGCCTTG TCCTAATAAA ATTAAGTTGC 
     4501 ATCATTTTGT CTGACTAGGT GTCCTTCTAT AATATTATGG GGTGGAGGGG GGTGGTATGG 
     4561 AGCAAGGGGC AAGTTGGGAA GACAACCTGT AGGGCCTGCG GGGTCTATTG GGAACCAAGC 
     4621 TGGAGTGCAG TGGCACAATC TTGGCTCACT GCAATCTCCG CCTCCTGGGT TCAAGCGATT 
     4681 CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA TTCCAGGCAT GCATGACCAG GCTCAGCTAA 
     4741 TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC ACCATATTGG CCAGGCTGGT CTCCAACTCC 
     4801 TAATCTCAGG TGATCTACCC ACCTTGGCCT CCCAAATTGC TGGGATTACA GGCGTGAACC 
     4861 ACTGCTCCCT TCCCTGTCCT TCTGATTTTA AAATAACTAT ACCAGCAGGA GGACGTCCAG 
     4921 ACACAGCATA GGCTACCTGG CCATGCCCAA CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC 
     4981 ACTGTCCTCT CATGCGTTGG GTCCACTCAG TAGATGCCTG TTGAATTGGG TACGCGGCCA 
     5041 GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA 
     5101 TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC 
     5161 CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG 
     5221 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG 
     5281 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT 
     5341 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT 
     5401 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG 
     5461 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT 
     5521 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT 
     5581 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT 
     5641 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC 
     5701 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC 
     5761 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG 
     5821 TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA 
     5881 AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA CCTGAGGCTA TGGCAGGGCC 
     5941 TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG GCCTTCACCC GAACTTGGGG GGTGGGGTGG 
     6001 GGAAAAGGAA GAAACGCGGG CGTATTGGCC CCAATGGGGT CTCGGTGGGG TATCGACAGA 
     6061 GTGCCAGCCC TGGGACCGAA CCCCGCGTTT ATGAACAAAC GACCCAACAC CGTGCGTTTT 
     6121 ATTCTGTCTT TTTATTGCCG TCATAGCGCG GGTTCCTTCC GGTATTGTCT CCTTCCGTGT 
     6181 TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA GAACTCCAGC ATGAGATCCC CGCGCTGGAG 
     6241 GATCATCCAG CCGGCGTCCC GGAAAACGAT TCCGAAGCCC AACCTTTCAT AGAAGGCGGC 
     6301 GGTGGAATCG AAATCTCGTG ATGGCAGGTT GGGCGTCGCT TGGTCGGTCA TTTCGAACCC 
     6361 CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA AGGCGATAGA AGGCGATGCG CTGCGAATCG 
     6421 GGAGCGGCGA TACCGTAAAG CACGAGGAAG CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA 
     6481 GCAATATCAC GGGTAGCCAA CGCTATGTCC TGATAGCGAT CCGCCACACC CAGCCGGCCA 
     6541 CAGTCGATGA ATCCAGAAAA GCGGCCATTT TCCACCATGA TATTCGGCAA GCAGGCATCG 
     6601 CCATGGGTCA CGACGAGATC CTCGCCGTCG GGCATGCTCG CCTTGAGCCT GGCGAACAGT 
     6661 TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG TCCAGATCAT CCTGATCGAC AAGACCGGCT 
     6721 TCCATCCGAG TACGTGCTCG CTCGATGCGA TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA 
     6781 GCCGGATCAA GCGTATGCAG CCGCCGCATT GCATCAGCCA TGATGGATAC TTTCTCGGCA 
     6841 GGAGCAAGGT GAGATGACAG GAGATCCTGC CCCGGCACTT CGCCCAATAG CAGCCAGTCC 
     6901 CTTCCCGCTT CAGTGACAAC GTCGAGCACA GCTGCGCAAG GAACGCCCGT CGTGGCCAGC 
     6961 CACGATAGCC GCGCTGCCTC GTCTTGCAGT TCATTCAGGG CACCGGACAG GTCGGTCTTG 
     7021 ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC AGCCGGAACA CGGCGGCATC AGAGCAGCCG 
     7081 ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT AGCCTCTCCA CCCAAGCGGC CGGAGAACCT 
     7141 GCGTGCAATC CATCTTGTTC AATCATGCGA AACGATCCTC ATCCTGTCTC TTGATCGATC 
     7201 TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC TCCTCACTAC TTCTGGAATA GCTCAGAGGC 
     7261 CGAGGCGGCC TCGGCCTCTG CATAAATAAA AAAAATTAGT CAGCCATGGG GCGGAGAATG 
     7321 GGCGGAACTG GGCGGAGTTA GGGGCGGGAT GGGCGGAGTT AGGGGCGGGA CTATGGTTGC 
     7381 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     7441 ACACCTGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     7501 TGGGGACTTT CCACACCCTA ACTGACACAC ATTCCACAGC TGGTTCTTTC CGCCTCAGGA 
     7561 CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC 
     7621 ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA 
     7681 GTGCCACCTG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC 
     7741 AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC 
     7801 TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG 
     7861 TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA 
     7921 CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC 
     7981 TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT 
     8041 TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA 
     8101 CAAAAATTTA ACGCGAATTT T
//