LOCUS       MR211593                8112 bp    DNA     circular  SYN 
DEFINITION  Helb (Myc-DDK-tagged) - Mouse helicase (DNA) B (Helb)
ACCESSION   NM_080446
VERSION     NM_080446.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4267..4358 
                     /label="Myc-DDK"  
     misc_feature    4388..4973 
                     /label="PolyA signal"  
     misc_feature    4445..4464 
                     /label="Restriction Enzyme"  
     misc_feature    5249..5921 
                     /label="ColE1"  
     misc_feature    6351..7145 
                     /label="Kan/Neo"  
     misc_feature    7180..7482 
                     /label="SV40 ori"  
     misc_feature    7568..8045 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4242..4249 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4242 
                     /label="Helb"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCTAGGCAG GATCGTCTGC GCGAGCTCCT GGGCCCTCTG CACCCGTACA 
     1081 AGAGCGACGA CGAGGAGGAG GATTGTGCGC AGGAGGAGGA GGGGGAGCAG GAGGAGGAGT 
     1141 TTGTGGATGC CGAGGAACTC TGCAGCGGGG GCATCAAGGC GGGCAGCCTC CCTGGGCGCG 
     1201 CGCGAGTGTC TATCCCTGAT GAGTATACTA AAGAGAAATG TACCGTGTAC GGACGTTTCC 
     1261 CACTCAAAGG TCCGTGGTGG CGCGTGAAGG TCCAGGTCCT GAAACCCCAG CGATCCAGGA 
     1321 GCTATCAAGT TCAAGGCTTT CCGGCATACT TTCTACAGGT TGATATGTCG CCACCAGATC 
     1381 AAAAACAAAT CTGTTCGCTT TTTCTTAAAG AATGCAACTT AGCCTCTGAG CGCATACAGG 
     1441 AATTTTTAAA ATGGGTAGAA AAAGTGTCAA GCTTTGAAAA TCTACACTTT GAAAATCTTT 
     1501 GGGAAACATT AAGGCTTTTC TACAGAGAAA CCGAAAAGAA AGATAAAAAG CTGTCTACAC 
     1561 CAAGGGAGCA GCAGGGTGAG GAGATGAGAG TGGAAAAATC ATTCGCATTT ATAAGTGCAA 
     1621 TGGTAGCTCT GCAATTCCCC AAAGTCATGG AATTCCTTCC ATCGCTTTTC CCTCGACACT 
     1681 TCAAAAGGCT CATCAGCTCG AGTTCTGATT GGGTGCTGGG ATGCATAGAA GATGTTCTGG 
     1741 GTACACAGCC GTGGAAGCTG GGCTTCAGGA GGATAACCTA CAGAGAAATG AAACTAGTGC 
     1801 GCTGTGAGGC CAGCTGGACA GCGTTCAGTC AGTGCCCCTC GCTGCTCCAG CTGATGACGC 
     1861 CCCTGCAGAA GAATGCATTG GTCATATATT CTAAGCTGAG GCAGACATGC AGAGAAGACG 
     1921 GACACACGTA CATTGAAGTA AAAGACTTGA CCTCGGGCTT GTCGGAGCAT ATGTCATTCG 
     1981 AGGAAGCGTG TCAGTCTCTG GCGTTTCTGA AGGACATTGA CGTGGTCATT TATGAGAAGG 
     2041 ACTATGTCTT TCTTTCTGAA CTGTACGAGG CCGAGCAAGA CATCGCCTCC TCCATCTGCG 
     2101 AGCTGATGAG CAGACCTCCG TGGCACTTAA AAGTGGACGT GAAGAACGTC CTGGCGTCAA 
     2161 TTAGAGGCGC AAAGCCCAAC GATCCAGGAA GTGCTGAGGC AGTGGAAGGG AGCAAGCCGG 
     2221 AGGAGGTGGG GTCAGAGCAG GGTGACAGTG TTTTAGACGC CCAAGATGGC GATGACCATG 
     2281 TTAGAAGCAA TGGCGAACAC GTCGCAAATG CAGAAATAAA CGACGTCCCA CTGGATCAAG 
     2341 ATCAGGTGGT CGCCCTGGAG ACGATCTGCG CCAACGCGGT GACTGTCCTC AGTGGGAAAG 
     2401 GCGGCTGTGG GAAGACCACC ATTGTTAGCC GTCTTTTTAA GCACATGGAA CATCTGGAAG 
     2461 AAACGGAAGT GCAGCAAGCC TGCGAGGATT TTGAACAAGA CCAGGAAGCC TCAGAAGAGT 
     2521 GGCTTGACTG TCCTAAGCAG AGCCCAGCCG GTGTGGACAA GGCTGTAGAA GTCTTGCTCA 
     2581 CCGCACCTAC AGGGAAGGCT GCTGGCTTAT TGAGGCAGAG GACCGATCTT CCGGCTTACA 
     2641 CGCTGTGCCA GGTCAATTAC AGCTTCTATA TGTGGAAAAC GAAAAATGAG GTGGACAAGC 
     2701 CGTGGAAATT CTCCACGGTT CGAGTTCTGG TTGTGGACGA AGGGAGTTTG GTGTCTGTAG 
     2761 GGATCTTCAA GTCAGTGTTA CAGTTGCTGT GCAAACACTC CAAGCTGTCC AAGCTCATCA 
     2821 TCCTTGGTGA TGTTAGACAG TTGCCCAGTA TTGAACCTGG TAACATGCTG CAGGATGTCT 
     2881 TTGAGACTCT TAAGTCAAGA CAGTGTGCCA TCGAACTAAA GACGAACCAC AGGACAGAGT 
     2941 CTCAGCTGAT TGTGGACAAT GCTACAAGAA TCTCCAGACG TCAGTTTCCA AAATTTGATG 
     3001 CTGAACTAAA TATCTGTGGT AATCCTACAT TACCTCTCTC CATTCAAGAT AAGACATTTA 
     3061 TCTTTGTGAG ACTCCCTGAA GAAGACAGCA GATCTCAGTC GTCTAAAGGT GAACACCGAT 
     3121 CTAATTTATA TACTGCAGTT AAAACTTTGC TTCAAGGAAA GGACTTCTGC TCTTTTGAAA 
     3181 GTTCAAAAAC ATCGCAATTT ATTGCATTTA GAAGGCAAGA CTGTGACCTC ATCAATGACT 
     3241 GCTGCTGCAA GCACTACACA GGCCACCTCA TCAAAGACCA TGAGAAGAAA CTCATCTTTG 
     3301 CCGTCGGTGA TAAGATCTGC TGTACCCGGA ATGCATACCT CTCAGATTTA CTCCCCGACA 
     3361 AAGACCAGGA AGCCGAGGGG AAAGGCTATG GAGACGCCCC TGACGACGAT GCTAAAATCA 
     3421 AACAGGATTT CGAAAGCAGC ACCCGCCTGT GCAACGGGGA AATATTTTTC ATAACACGGG 
     3481 ATGTAACTGA CGTAACTTTC AAAAGAAAAA GACTTCTGAC CATTAATAAC GAGGCCGGTT 
     3541 TGGAAGTGAC TGTGGACTTT AGCAAACTAA TGGCAAACTG TCAGATCAAG CATGCCTGGG 
     3601 CAAGGACCAT TCATACTTTT CAGGGGTCCG AGGAGAACAC AGTCGTCTAC GTCGTGGGGA 
     3661 AGGCAGGCCG CCAGCACTGG CAACATGTTT ACACGGCAGT GACCCGGGGC CGCTCCAGAG 
     3721 TGTACATCAT CGCGCAGGAG TCTGAACTCA GGAGTGCCAC CCGGAAGCGC GGTTTCCCCA 
     3781 GGCAGACGCG TTTGAAACAC TTCTTGCAGA AGAAGCTCTC TGGCAGCTGC GCACCCTCAA 
     3841 CAGGTTTCGC ATCCCAGCCT TCGAGCCCTC GAGTCGGAGG AAGACCTGAC ACCCAGCCTC 
     3901 CAGCGTCACA CCTCTGTAGA ACCCCAGACA ACAAAGCCAC TGCAGACAGC GCCAGAGGCG 
     3961 ACGAAAGATG GCTGTCAGCT TCTGTTAACG ATGACGTGGA TACGGATGAG GAGTCAGCAC 
     4021 AGCTCAGAGG GTCCAAAAGA ATCGGTGACG GCTTCCCGTT TGATGAAGAA AGCCCCAGTA 
     4081 AATTCCGTAT GGTTGAAGCG CCATCTCCAC AGGTGTCCTC TGTGTTTCAG AATATGAGGT 
     4141 TGAATACTCT CACTCCCAGA CAGCTTTTTA AACCCACCGA CAATCAAGAC ACAGGTACGG 
     4201 CAGGTGTGGC GGACGACGCC AACGATCCCA GCAATCAGGA GATGGAAATG AGCGGACCGA 
     4261 CGCGTACGCG GCCGCTCGAG CAGAAACTCA TCTCAGAAGA GGATCTGGCA GCAAATGATA 
     4321 TCCTGGATTA CAAGGATGAC GACGATAAGG TTTAAACGGC CGGCCGCGGT CATAGCTGTT 
     4381 TCCTGAACAG ATCCCGGGTG GCATCCCTGT GACCCCTCCC CAGTGCCTCT CCTGGCCCTG 
     4441 GAAGTTGCCA CTCCAGTGCC CACCAGCCTT GTCCTAATAA AATTAAGTTG CATCATTTTG 
     4501 TCTGACTAGG TGTCCTTCTA TAATATTATG GGGTGGAGGG GGGTGGTATG GAGCAAGGGG 
     4561 CAAGTTGGGA AGACAACCTG TAGGGCCTGC GGGGTCTATT GGGAACCAAG CTGGAGTGCA 
     4621 GTGGCACAAT CTTGGCTCAC TGCAATCTCC GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT 
     4681 CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA TGCATGACCA GGCTCAGCTA ATTTTTGTTT 
     4741 TTTTGGTAGA GACGGGGTTT CACCATATTG GCCAGGCTGG TCTCCAACTC CTAATCTCAG 
     4801 GTGATCTACC CACCTTGGCC TCCCAAATTG CTGGGATTAC AGGCGTGAAC CACTGCTCCC 
     4861 TTCCCTGTCC TTCTGATTTT AAAATAACTA TACCAGCAGG AGGACGTCCA GACACAGCAT 
     4921 AGGCTACCTG GCCATGCCCA ACCGGTGGGA CATTTGAGTT GCTTGCTTGG CACTGTCCTC 
     4981 TCATGCGTTG GGTCCACTCA GTAGATGCCT GTTGAATTGG GTACGCGGCC AGCGGCGAGC 
     5041 GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG 
     5101 AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT 
     5161 GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA 
     5221 GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT 
     5281 CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC 
     5341 GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT 
     5401 TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC 
     5461 CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC 
     5521 CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG 
     5581 GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC 
     5641 AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG 
     5701 CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA 
     5761 TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT 
     5821 TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG 
     5881 TTTTAAATCA ATCTAAAGTA TATATGAGTA ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC 
     5941 GACGTTGGCT GCGAGCCCTG GGCCTTCACC CGAACTTGGG GGGTGGGGTG GGGAAAAGGA 
     6001 AGAAACGCGG GCGTATTGGC CCCAATGGGG TCTCGGTGGG GTATCGACAG AGTGCCAGCC 
     6061 CTGGGACCGA ACCCCGCGTT TATGAACAAA CGACCCAACA CCGTGCGTTT TATTCTGTCT 
     6121 TTTTATTGCC GTCATAGCGC GGGTTCCTTC CGGTATTGTC TCCTTCCGTG TTTCAGTTAG 
     6181 CCTCCCCCTA GGGTGGGCGA AGAACTCCAG CATGAGATCC CCGCGCTGGA GGATCATCCA 
     6241 GCCGGCGTCC CGGAAAACGA TTCCGAAGCC CAACCTTTCA TAGAAGGCGG CGGTGGAATC 
     6301 GAAATCTCGT GATGGCAGGT TGGGCGTCGC TTGGTCGGTC ATTTCGAACC CCAGAGTCCC 
     6361 GCTCAGAAGA ACTCGTCAAG AAGGCGATAG AAGGCGATGC GCTGCGAATC GGGAGCGGCG 
     6421 ATACCGTAAA GCACGAGGAA GCGGTCAGCC CATTCGCCGC CAAGCTCTTC AGCAATATCA 
     6481 CGGGTAGCCA ACGCTATGTC CTGATAGCGA TCCGCCACAC CCAGCCGGCC ACAGTCGATG 
     6541 AATCCAGAAA AGCGGCCATT TTCCACCATG ATATTCGGCA AGCAGGCATC GCCATGGGTC 
     6601 ACGACGAGAT CCTCGCCGTC GGGCATGCTC GCCTTGAGCC TGGCGAACAG TTCGGCTGGC 
     6661 GCGAGCCCCT GATGCTCTTC GTCCAGATCA TCCTGATCGA CAAGACCGGC TTCCATCCGA 
     6721 GTACGTGCTC GCTCGATGCG ATGTTTCGCT TGGTGGTCGA ATGGGCAGGT AGCCGGATCA 
     6781 AGCGTATGCA GCCGCCGCAT TGCATCAGCC ATGATGGATA CTTTCTCGGC AGGAGCAAGG 
     6841 TGAGATGACA GGAGATCCTG CCCCGGCACT TCGCCCAATA GCAGCCAGTC CCTTCCCGCT 
     6901 TCAGTGACAA CGTCGAGCAC AGCTGCGCAA GGAACGCCCG TCGTGGCCAG CCACGATAGC 
     6961 CGCGCTGCCT CGTCTTGCAG TTCATTCAGG GCACCGGACA GGTCGGTCTT GACAAAAAGA 
     7021 ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC ACGGCGGCAT CAGAGCAGCC GATTGTCTGT 
     7081 TGTGCCCAGT CATAGCCGAA TAGCCTCTCC ACCCAAGCGG CCGGAGAACC TGCGTGCAAT 
     7141 CCATCTTGTT CAATCATGCG AAACGATCCT CATCCTGTCT CTTGATCGAT CTTTGCAAAA 
     7201 GCCTAGGCCT CCAAAAAAGC CTCCTCACTA CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC 
     7261 CTCGGCCTCT GCATAAATAA AAAAAATTAG TCAGCCATGG GGCGGAGAAT GGGCGGAACT 
     7321 GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT TAGGGGCGGG ACTATGGTTG CTGACTAATT 
     7381 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCTGGT 
     7441 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     7501 TCCACACCCT AACTGACACA CATTCCACAG CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT 
     7561 TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA 
     7621 TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT 
     7681 GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC 
     7741 GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC 
     7801 ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT 
     7861 AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG 
     7921 CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT 
     7981 GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA 
     8041 TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT 
     8101 AACGCGAATT TT
//