LOCUS       MR211589                8100 bp    DNA     circular  SYN 
DEFINITION  Ret (Myc-DDK-tagged) - Mouse ret proto-oncogene (Ret), transcript variant 4
ACCESSION   NM_001080780
VERSION     NM_001080780.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4257..4348 
                     /label="Myc-DDK"  
     misc_feature    4378..4963 
                     /label="PolyA signal"  
     misc_feature    4435..4454 
                     /label="Restriction Enzyme"  
     misc_feature    5239..5911 
                     /label="ColE1"  
     misc_feature    6341..7135 
                     /label="Kan/Neo"  
     misc_feature    7170..7472 
                     /label="SV40 ori"  
     misc_feature    7558..8035 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4239..4245 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4239 
                     /label="Ret"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGAAAGCG ACGTCCGGCG CCGCAGGGCT GGGGCTGAAG CTGATTTTGC 
     1081 TCCTGCCGCT GCTAGGAGAA GCCCCACTGG GCCTCTATTT CTCAAGGGAT GCTTACTGGG 
     1141 AGAGGCTGTA TGTAGACCAG CCAGCTGGCA CACCTCTGCT CTATGTCCAT GCCCTACGGG 
     1201 ATGCCCCTGG AGAAGTGCCG AGCTTCCGCC TGGGCCAGCA TCTCTATGGC GTCTACCGTA 
     1261 CACGGCTGCA TGAGAATGAC TGGATCCGCA TCAATGAGAC TACTGGCCTT CTCTACCTCA 
     1321 ATCAGAGCCT GGACCACAGT TCCTGGGAAC AGCTCAGCAT CCGCAATGGT GGTTTCCCCC 
     1381 TGCTCACCAT CTTCCTCCAG GTCTTTCTGG GGTCCACAGC CCAGAGAGAG GGAGAATGCC 
     1441 ATTGGCCAGG CTGTACCCGT GTGTACTTCT CCTTCATCAA CGACACCTTC CCAAATTGTA 
     1501 GCTCCTTCAA AGCCCAGGAT CTCTGCATCC CAGAGACAGC CGTGTCCTTC CGAGTCAGGG 
     1561 AGAACAGGCC TCCTGGCACC TTCTACCACT TCCACATGTT ACCCGTGCAG TTCCTTTGTC 
     1621 CCAACATCAG TGTGAAGTAC AGTCTCTTAG GAGGGGATAG TCTGCCCTTC CGTTGTGACC 
     1681 CAGACTGCCT GGAGGTGAGC ACTCGCTGGG CCCTGGATCG AGAGCTCCGG GAGAAGTATG 
     1741 TGCTGGAGGC TTTGTGCATA GTGGCAGGCC CTGGTGCCAA CAAAGAGACG GTGACTCTGT 
     1801 CCTTCCCAGT GACAGTGTAT GATGAGGACG ACTCGGCGCC CACCTTCTCT GGAGGTGTGG 
     1861 GCACTGCCAG CGCGGTGGTG GAGTTTAAGC GGAAGGAGGG CACTGTGGTG GCCACCCTGC 
     1921 AGGTGTTCGA TGCAGATGTG GTGCCAGCGT CTGGGGAGCT GGTGAGACGG TACACAAACA 
     1981 CACTCCTCTC AGGGGACTCC TGGGCCCAGC AGACCTTCCG GGTGGAGCAT TCGCCCATCG 
     2041 AGACCTTGGT CCAGGTCAAC AACAACTCCG TTCGGGCAAC CATGCACAAT TACAAGCTGA 
     2101 TTCTCAACAG GAGCCTGTCT ATCTCAGAGA GCCGAGTCCT GCAGCTCGCG GTCCTGGTCA 
     2161 ACGACTCAGA CTTCCAGGGG CCTGGGGCAG GTGGTATCCT CGTCCTCCAT TTCAACGTGT 
     2221 CTGTACTGCC CGTCACCCTG AACCTACCCA GGGCCTACTC CTTCCCAGTG AATAAGAGGG 
     2281 CCCGCCGCTA TGCCCAGATC GGGAAAGTCT GTGTGGAAAA CTGCCAGGAG TTCAGCGGTG 
     2341 TCTCCATCCA GTACAAGCTG CAGCCTTCCA GCATCAACTG CACTGCCCTA GGTGTGGTCA 
     2401 CCTCACCCGA GGACACCTCG GGGACCCTAT TTGTAAATGA CACAGAGGCC CTGCGGCGAC 
     2461 CTGAGTGCAC CAAGCTTCAG TACACGGTGG TAGCCACTGA CCGGCAGACC CGCAGACAGA 
     2521 CCCAGGCTTC GCTAGTGGTC ACTGTGGAGG GGACATCCAT TACTGAAGAA GTAGGCTGCC 
     2581 CCAAGTCCTG TGCAGTAAAC AAGAGGCGCC CCGAGTGTGA GGAATGTGGT GGCCTGGGTT 
     2641 CTCCAACTGG CAGGTGCGAG TGGCGCCAGG GAGATGGTAA AGGGATCACC AGGAACTTCT 
     2701 CCACCTGCTC CCCCAGTACC AGGACCTGCC CCGACGGCCA CTGTGATGCT GTGGAGAGCC 
     2761 GGGATGCCAA CATTTGCCCC CAGGACTGTC TCCGTGCCGA CATTGTTGGA GGACACGAGC 
     2821 GAGGGGAGCG CCAGGGCATT AAAGCAGGCT ACGGCATCTG CAACTGTTTC CCTGATGAGA 
     2881 AGAAATGCTT CTGCGAGCCA GAGGACAGCC AGGGCCCACT GTGTGATGCG CTGTGCCGCA 
     2941 CGATCATCAC AGCTGCCCTC TTCTCCCTTA TCATCTCCAT CCTGCTGTCC ATCTTCTGTG 
     3001 TCTGCCACCA CCACAAGCAT GGGCACAAGC CGCCCATTGC ATCAGCGGAA ATGACCTTCT 
     3061 GCCGGCCGGC CCAGGGCTTC CCAATCAGTT ATTCCTCCTC AGGCACCCGC CGGCCCTCAC 
     3121 TGGATTCCAC GGAGAACCAG GTTCCTGTGG ACTCTTTCAA GATCCCGGAG GATCCAAAGT 
     3181 GGGAATTTCC TCGGAAGAAC TTAGTTCTTG GGAAAACTCT GGGAGAAGGC GAGTTTGGAA 
     3241 AAGTTGTCAA GGCCACAGCC TTCCGTCTGA AAGGCCGGGC AGGATACACC ACAGTGGCTG 
     3301 TGAAAATGCT GAAAGAAAAC GCCTCCCAGA GTGAGTTACG AGACCTGCTG TCTGAGTTCA 
     3361 ACCTTCTGAA ACAAGTCAAC CATCCACATG TCATCAAGTT GTATGGGGCC TGCAGCCAGG 
     3421 ATGGGCCACT TCTTCTCATT GTGGAGTATG CCAAGTATGG CTCCCTGCGG GGATTCCTCC 
     3481 GTGACAGCCG CAAGATTGGG CCTGCCTATG TGAGCGGTGG AGGCAGCCGC AACTCCAGCT 
     3541 CGCTGGACCA CCCAGATGAA AGGGTACTGA CCATGGGTGA CCTCATCTCC TTCGCCTGGC 
     3601 AGATCTCGAG GGGTATGCAG TACTTGGCAG AAATGAAGCT TGTACATCGG GACTTAGCTG 
     3661 CCAGGAACAT CTTGGTGGCT GAGGGACGGA AGATGAAGAT TTCCGACTTT GGGCTGTCCC 
     3721 GAGATGTTTA TGAGGAAGAT TCCTATGTGA AGAAAAGCAA GGGCCGGATT CCCGTCAAGT 
     3781 GGATGGCAAT TGAGTCCCTT TTCGATCACA TCTATACTAC TCAAAGTGAT GTGTGGTCCT 
     3841 TTGGAGTGCT GCTCTGGGAG ATTGTGACCC TGGGAGGCAA CCCCTACCCT GGAATTCCTC 
     3901 CTGAACGACT CTTCAACCTT CTGAAGACAG GCCACAGGAT GGAGAGGCCA GACAACTGCA 
     3961 GCGAGGAAAT GTACCGTCTG ATGCTGCAGT GCTGGAAGCA GGAGCCAGAC AAGAGGCCAG 
     4021 TGTTTGCTGA CATCAGCAAG GATCTGGAGA AGATGATGGT CAAGAGCAGA GACTACTTGG 
     4081 ACCTGGCTGC ATCCACACCT TCGGACTCAC TGCTGTATGA CGATGGGCTC TCAGAAGAGG 
     4141 AGACACCCCT GGTGGACTGT AACAATGCTC CCCTCCCGCG CTCCCTCCCT TCCACATGGA 
     4201 TTGAAAACAA ACTCTATGGT AGAATTTCAC ATGCATTTAC TAGATTCACG CGTACGCGGC 
     4261 CGCTCGAGCA GAAACTCATC TCAGAAGAGG ATCTGGCAGC AAATGATATC CTGGATTACA 
     4321 AGGATGACGA CGATAAGGTT TAAACGGCCG GCCGCGGTCA TAGCTGTTTC CTGAACAGAT 
     4381 CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA GTGCCTCTCC TGGCCCTGGA AGTTGCCACT 
     4441 CCAGTGCCCA CCAGCCTTGT CCTAATAAAA TTAAGTTGCA TCATTTTGTC TGACTAGGTG 
     4501 TCCTTCTATA ATATTATGGG GTGGAGGGGG GTGGTATGGA GCAAGGGGCA AGTTGGGAAG 
     4561 ACAACCTGTA GGGCCTGCGG GGTCTATTGG GAACCAAGCT GGAGTGCAGT GGCACAATCT 
     4621 TGGCTCACTG CAATCTCCGC CTCCTGGGTT CAAGCGATTC TCCTGCCTCA GCCTCCCGAG 
     4681 TTGTTGGGAT TCCAGGCATG CATGACCAGG CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA 
     4741 CGGGGTTTCA CCATATTGGC CAGGCTGGTC TCCAACTCCT AATCTCAGGT GATCTACCCA 
     4801 CCTTGGCCTC CCAAATTGCT GGGATTACAG GCGTGAACCA CTGCTCCCTT CCCTGTCCTT 
     4861 CTGATTTTAA AATAACTATA CCAGCAGGAG GACGTCCAGA CACAGCATAG GCTACCTGGC 
     4921 CATGCCCAAC CGGTGGGACA TTTGAGTTGC TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG 
     4981 TCCACTCAGT AGATGCCTGT TGAATTGGGT ACGCGGCCAG CGGCGAGCGG TATCAGCTCA 
     5041 CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG 
     5101 AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA 
     5161 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA 
     5221 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC 
     5281 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC 
     5341 GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT 
     5401 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG 
     5461 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG 
     5521 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA 
     5581 CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG 
     5641 AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT 
     5701 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT 
     5761 TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG 
     5821 ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT 
     5881 CTAAAGTATA TATGAGTAAC CTGAGGCTAT GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC 
     5941 GAGCCCTGGG CCTTCACCCG AACTTGGGGG GTGGGGTGGG GAAAAGGAAG AAACGCGGGC 
     6001 GTATTGGCCC CAATGGGGTC TCGGTGGGGT ATCGACAGAG TGCCAGCCCT GGGACCGAAC 
     6061 CCCGCGTTTA TGAACAAACG ACCCAACACC GTGCGTTTTA TTCTGTCTTT TTATTGCCGT 
     6121 CATAGCGCGG GTTCCTTCCG GTATTGTCTC CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG 
     6181 GTGGGCGAAG AACTCCAGCA TGAGATCCCC GCGCTGGAGG ATCATCCAGC CGGCGTCCCG 
     6241 GAAAACGATT CCGAAGCCCA ACCTTTCATA GAAGGCGGCG GTGGAATCGA AATCTCGTGA 
     6301 TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT TTCGAACCCC AGAGTCCCGC TCAGAAGAAC 
     6361 TCGTCAAGAA GGCGATAGAA GGCGATGCGC TGCGAATCGG GAGCGGCGAT ACCGTAAAGC 
     6421 ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA AGCTCTTCAG CAATATCACG GGTAGCCAAC 
     6481 GCTATGTCCT GATAGCGATC CGCCACACCC AGCCGGCCAC AGTCGATGAA TCCAGAAAAG 
     6541 CGGCCATTTT CCACCATGAT ATTCGGCAAG CAGGCATCGC CATGGGTCAC GACGAGATCC 
     6601 TCGCCGTCGG GCATGCTCGC CTTGAGCCTG GCGAACAGTT CGGCTGGCGC GAGCCCCTGA 
     6661 TGCTCTTCGT CCAGATCATC CTGATCGACA AGACCGGCTT CCATCCGAGT ACGTGCTCGC 
     6721 TCGATGCGAT GTTTCGCTTG GTGGTCGAAT GGGCAGGTAG CCGGATCAAG CGTATGCAGC 
     6781 CGCCGCATTG CATCAGCCAT GATGGATACT TTCTCGGCAG GAGCAAGGTG AGATGACAGG 
     6841 AGATCCTGCC CCGGCACTTC GCCCAATAGC AGCCAGTCCC TTCCCGCTTC AGTGACAACG 
     6901 TCGAGCACAG CTGCGCAAGG AACGCCCGTC GTGGCCAGCC ACGATAGCCG CGCTGCCTCG 
     6961 TCTTGCAGTT CATTCAGGGC ACCGGACAGG TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC 
     7021 TGCGCTGACA GCCGGAACAC GGCGGCATCA GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA 
     7081 TAGCCGAATA GCCTCTCCAC CCAAGCGGCC GGAGAACCTG CGTGCAATCC ATCTTGTTCA 
     7141 ATCATGCGAA ACGATCCTCA TCCTGTCTCT TGATCGATCT TTGCAAAAGC CTAGGCCTCC 
     7201 AAAAAAGCCT CCTCACTACT TCTGGAATAG CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC 
     7261 ATAAATAAAA AAAATTAGTC AGCCATGGGG CGGAGAATGG GCGGAACTGG GCGGAGTTAG 
     7321 GGGCGGGATG GGCGGAGTTA GGGGCGGGAC TATGGTTGCT GACTAATTGA GATGCATGCT 
     7381 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCTGGTTG CTGACTAATT 
     7441 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCCTAA 
     7501 CTGACACACA TTCCACAGCT GGTTCTTTCC GCCTCAGGAC TCTTCCTTTT TCAATATTAT 
     7561 TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA 
     7621 AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA CGCGCCCTGT 
     7681 AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC 
     7741 AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC 
     7801 TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG 
     7861 CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA 
     7921 TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC 
     7981 CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT TTGATTTATA AGGGATTTTG 
     8041 CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//