LOCUS       MR211580                8091 bp    DNA     circular  SYN 
DEFINITION  Ube3b (Myc-DDK-tagged) - Mouse ubiquitin protein ligase E3B (Ube3b)
ACCESSION   NM_054093
VERSION     NM_054093.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4248..4339 
                     /label="Myc-DDK"  
     misc_feature    4369..4954 
                     /label="PolyA signal"  
     misc_feature    4426..4445 
                     /label="Restriction Enzyme"  
     misc_feature    5230..5902 
                     /label="ColE1"  
     misc_feature    6332..7126 
                     /label="Kan/Neo"  
     misc_feature    7161..7463 
                     /label="SV40 ori"  
     misc_feature    7549..8026 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4230..4236 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4230 
                     /label="Ube3b"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTTCACTGTA TCTCAGACCT CCAGAGCATG GTTCATCGAC AGAGCCCGGC 
     1081 AGGCACGAGA AGAGAGGCTG GTGCAGAAGG AGCGGGAGCG CTCAGCGGTG ACGATCCAGG 
     1141 CCCTAGTCCG CAGTTTCCTC TGCCGGAGGC GACTGCACAG AGACATCAGG AAAGAGATTG 
     1201 ATGAGTTTTT TAGTGCTGAT GAGTCTGGGT CCAGTAAAAG AAGTGCACTT TGTATTTTTA 
     1261 AGATTGCCAG GAGACTGCTG TTTATATGCA AAACTACCGA GGACAGCGAG AGACTGGAGA 
     1321 AGTTGTGTCG AAGCATCCTG AACAGCATGG ATGCCGAGAA TGAGCCAAAG GTGTGGTACG 
     1381 TGTCCCTGGC TCTCTCCAAG GACCTCACGC TGCTGTGGAT TAAGCAGATC AAGAGCATCC 
     1441 TGTGGCACTG CTGTGAGCTT CTGGGGCAGC TCAAGCCAGA GATCTTACAG GACTCCAGGC 
     1501 TCATCACGCT GTACCTCACC ATGCTTGTGA CCTTCACAGA CACTTCAACA TGGAAGATTC 
     1561 TCCGGGGAAA AGGTGAGAGC CTTCGGCCAG CCCTGAACCA CATTTGCGCA AATATAATGG 
     1621 GCCATCTCAA CCAGCGTGGA CTTTACTCTG TATTGCAGGT ACTGCTGACC CGAGGCCTGG 
     1681 CCAGGCCCCG GCCCTGTCTG TCCAAAGGCA TGTTAACAGC AGCCTTCTCT CTGGCCCTGC 
     1741 GCCCTGTGGT TGCTGCGCAG TTCTCAGACA ATCTTATGCG ACCATTCATC ATCCACGTCA 
     1801 TGTCGGTACC TGCTCTCGTG GCTCATCTCA GCACCGTGGC CCCTGAGCGC CTCGGCGTTT 
     1861 TAGAGTCCCA TGACATGCTT CGTAAATTCA TCGTCTTCCT GAGAGATAGA GACCGATGCC 
     1921 GCGATGCTTG TGAGAGTTTA GAAGGCTGCC ATACCCTGTG TCTCATGGGC AATCTCCTGC 
     1981 ACTTGGGCTC CCTCAGCCTC AGGCTGTTAG AGGAAGAGAT GGATGGGTTT GTGAGCGCGC 
     2041 TCACGCAGAT GCTGTGCTAC TGTCAGAAGT ACGTGGCTCA GAAGAAGTCC AACCTGACCC 
     2101 ACTGGCACCC TGTGCTCGGC TGGTTTTCCC AACCTGTGGA TTACGGCCTC AATGATTCCA 
     2161 TGTACCTGAT CACCAAGCAG CTGCAGTTCC TGTGGGCGGT GCCTCTCATC CGCATTCTCT 
     2221 TCAGTGACAT CCTGAGCAGG AAGCTGCTGG AGCATGCAGA GCCGGCCCCG GTGCAACCGC 
     2281 AGCCGTCCTC ACCGCAGACG GTGCTCCCCG TGAAGAGCCT CCTCAAGCGT GCGTTTCAGA 
     2341 AGTCGGCTTC GGTTCGGAAC ATCCTCAGGC CTGTTGGGGG CCGCCGTGTC GACTCCGCTG 
     2401 AGGTGCGGAA GGTCTGCAAC ATTTGCGTGC TCTACCAGAC CTCGCTGACG ACCCTCACCC 
     2461 AGATCCGCCT GCAGATCCTC ACAGGTCTTA CGTACCTGGA TGACCTGCTG CCCAAGCTCT 
     2521 GGGCGTTTAT CTGTGAGCTG GGGCCCCACG GCGGGCTCAA GCTCTTCCTG GAGTGCCTGA 
     2581 ACAATGACAC TGGAGAGTCC AAGCAGCTCC TGGCCATGCT GATGCTCTTC TGTGACTGTT 
     2641 CCCGGCACCT CATCACCATC CTCGATGACA TTGAAGTTTA TGAAGAACAG ATCTCCTTCA 
     2701 AGCTGGAAGA GCTGGTCACC ATCTCCTCGT TCCTCAATTC CTTCGTGTTT AAGATGATCT 
     2761 GGGACGGAAT TGTAGAGAAT GCCAAGGGTG AGACCTTGGA GCTGTTCCAG TCCGTCCATG 
     2821 GCTGGCTGAT GGTGCTGTAT GAGCGAGACT GCCGCCGGCG CTTTGCTCCG GAGGACCACT 
     2881 GGCTACGGAG GGACCTCAAG CCTGGTGTGC TCTTCCAGGA GCTCGACAAG GACCGAAGGA 
     2941 GGGCTCAGCT GGTCCTGCAG CACATCCCCC ACGTCGTGCC TCACAAGAAC AGAGTTCTTC 
     3001 TGTTCCGGAA CATGGTTATC AAGGAGAAAG AGAAGCTGGG GCTTGTGGAG ACCAGCTCTG 
     3061 CCTCTCCTCA CGTCACCCAC ATCACCATCC GAAGGTCCCG GATGTTGGAG GACGGCTACG 
     3121 AACAGCTCCG GCAGCTTTCC CAGCATGCCA TGAAGGGTGT GATCCGAGTG AAGTTTGTCA 
     3181 ACGACCTCGG GGTGGACGAG GCTGGGATTG ACCAGGATGG TGTCTTCAAG GAGTTTCTGG 
     3241 AAGAGATCAT CAAGAGGGTG TTTGACCCCG CACTCAACCT GTTCAAGACA ACCAGCGGGG 
     3301 ATGAGCGGCT GTACCCATCA CCCACGTCCT ACATTCACGA GAATTACCTG CAGCTCTTCG 
     3361 AGTTTGTGGG GAAGATGCTG GGAAAGGCAG TGTATGAGGG GATCGTGGTA GATGTGCCCT 
     3421 TTGCCTCCTT CTTCCTGAGC CAGATGCTCG GGCACCACCA CAGCGTCTTC TACAGCTCCG 
     3481 TGGATGAGCT TCCCTCCCTG GACTCGGAGT TTTATAAAAA CCTCACCTCC ATCAAGCGCT 
     3541 ACGATGGAGA CATCGCAGAC CTCGGGCTGA CGCTGTCTTA CGATGAAGAC GTCATGGGTC 
     3601 AGCTCGTCTG CCACGAACTG GTTCCCGGAG GGAAGACCAT CCCGGTTACA GATGAGAACA 
     3661 AAATTAGCTA CATCCATCTG ATGGCGCATT TCCGAATGCA CACTCAAATT AAAAACCAGA 
     3721 CAGCTGCTCT TATCAGTGGC TTCCGTTCCA TCATCAAGCC CGAGTGGATC CGAATGTTCT 
     3781 CTACCCCTGA GCTTCAGCGC CTCATCTCCG GGGACAACGC TGAGATAGAT CTGGAAGACT 
     3841 TAAAGAAGCA CACAGTGTAC TACGGTGGCT TTCATGGGAG CCACAGGGTC ATCATCTGGC 
     3901 TCTGGGATAT CCTGGCTTCC GACTTCACGC CTGAAGAAAG AGCCATGTTC CTGAAGTTTG 
     3961 TGACCAGCTG CTCCCGGCCC CCGCTCCTAG GATTCGCCTA CCTCAAGCCT CCGTTCTCCA 
     4021 TCCGCTGTGT GGAAGTATCA GATGACCAGG ACACTGGGGA TACCCTGGGC AGCGTCCTCA 
     4081 GGGGCTTCTT CACCATCCGC AAGAGGGAGC CAGGGGGGCG TCTGCCCACA TCTTCCACTT 
     4141 GCTTCAACCT GCTTAAGCTG CCCAACTACA GCAAGAAGAG CGTACTGCGA GAGAAACTAC 
     4201 GCTATGCCAT CAGCATGAAC ACGGGCTTTG AGCTCTCCAC GCGTACGCGG CCGCTCGAGC 
     4261 AGAAACTCAT CTCAGAAGAG GATCTGGCAG CAAATGATAT CCTGGATTAC AAGGATGACG 
     4321 ACGATAAGGT TTAAACGGCC GGCCGCGGTC ATAGCTGTTT CCTGAACAGA TCCCGGGTGG 
     4381 CATCCCTGTG ACCCCTCCCC AGTGCCTCTC CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC 
     4441 ACCAGCCTTG TCCTAATAAA ATTAAGTTGC ATCATTTTGT CTGACTAGGT GTCCTTCTAT 
     4501 AATATTATGG GGTGGAGGGG GGTGGTATGG AGCAAGGGGC AAGTTGGGAA GACAACCTGT 
     4561 AGGGCCTGCG GGGTCTATTG GGAACCAAGC TGGAGTGCAG TGGCACAATC TTGGCTCACT 
     4621 GCAATCTCCG CCTCCTGGGT TCAAGCGATT CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA 
     4681 TTCCAGGCAT GCATGACCAG GCTCAGCTAA TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC 
     4741 ACCATATTGG CCAGGCTGGT CTCCAACTCC TAATCTCAGG TGATCTACCC ACCTTGGCCT 
     4801 CCCAAATTGC TGGGATTACA GGCGTGAACC ACTGCTCCCT TCCCTGTCCT TCTGATTTTA 
     4861 AAATAACTAT ACCAGCAGGA GGACGTCCAG ACACAGCATA GGCTACCTGG CCATGCCCAA 
     4921 CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC ACTGTCCTCT CATGCGTTGG GTCCACTCAG 
     4981 TAGATGCCTG TTGAATTGGG TACGCGGCCA GCGGCGAGCG GTATCAGCTC ACTCAAAGGC 
     5041 GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG 
     5101 CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG 
     5161 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG 
     5221 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC 
     5281 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA 
     5341 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT 
     5401 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC 
     5461 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG 
     5521 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC 
     5581 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT 
     5641 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA 
     5701 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG 
     5761 GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA 
     5821 AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT 
     5881 ATATGAGTAA CCTGAGGCTA TGGCAGGGCC TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG 
     5941 GCCTTCACCC GAACTTGGGG GGTGGGGTGG GGAAAAGGAA GAAACGCGGG CGTATTGGCC 
     6001 CCAATGGGGT CTCGGTGGGG TATCGACAGA GTGCCAGCCC TGGGACCGAA CCCCGCGTTT 
     6061 ATGAACAAAC GACCCAACAC CGTGCGTTTT ATTCTGTCTT TTTATTGCCG TCATAGCGCG 
     6121 GGTTCCTTCC GGTATTGTCT CCTTCCGTGT TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA 
     6181 GAACTCCAGC ATGAGATCCC CGCGCTGGAG GATCATCCAG CCGGCGTCCC GGAAAACGAT 
     6241 TCCGAAGCCC AACCTTTCAT AGAAGGCGGC GGTGGAATCG AAATCTCGTG ATGGCAGGTT 
     6301 GGGCGTCGCT TGGTCGGTCA TTTCGAACCC CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA 
     6361 AGGCGATAGA AGGCGATGCG CTGCGAATCG GGAGCGGCGA TACCGTAAAG CACGAGGAAG 
     6421 CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA GCAATATCAC GGGTAGCCAA CGCTATGTCC 
     6481 TGATAGCGAT CCGCCACACC CAGCCGGCCA CAGTCGATGA ATCCAGAAAA GCGGCCATTT 
     6541 TCCACCATGA TATTCGGCAA GCAGGCATCG CCATGGGTCA CGACGAGATC CTCGCCGTCG 
     6601 GGCATGCTCG CCTTGAGCCT GGCGAACAGT TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG 
     6661 TCCAGATCAT CCTGATCGAC AAGACCGGCT TCCATCCGAG TACGTGCTCG CTCGATGCGA 
     6721 TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA GCCGGATCAA GCGTATGCAG CCGCCGCATT 
     6781 GCATCAGCCA TGATGGATAC TTTCTCGGCA GGAGCAAGGT GAGATGACAG GAGATCCTGC 
     6841 CCCGGCACTT CGCCCAATAG CAGCCAGTCC CTTCCCGCTT CAGTGACAAC GTCGAGCACA 
     6901 GCTGCGCAAG GAACGCCCGT CGTGGCCAGC CACGATAGCC GCGCTGCCTC GTCTTGCAGT 
     6961 TCATTCAGGG CACCGGACAG GTCGGTCTTG ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC 
     7021 AGCCGGAACA CGGCGGCATC AGAGCAGCCG ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT 
     7081 AGCCTCTCCA CCCAAGCGGC CGGAGAACCT GCGTGCAATC CATCTTGTTC AATCATGCGA 
     7141 AACGATCCTC ATCCTGTCTC TTGATCGATC TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC 
     7201 TCCTCACTAC TTCTGGAATA GCTCAGAGGC CGAGGCGGCC TCGGCCTCTG CATAAATAAA 
     7261 AAAAATTAGT CAGCCATGGG GCGGAGAATG GGCGGAACTG GGCGGAGTTA GGGGCGGGAT 
     7321 GGGCGGAGTT AGGGGCGGGA CTATGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     7381 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCTGGTT GCTGACTAAT TGAGATGCAT 
     7441 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCCTA ACTGACACAC 
     7501 ATTCCACAGC TGGTTCTTTC CGCCTCAGGA CTCTTCCTTT TTCAATATTA TTGAAGCATT 
     7561 TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA 
     7621 ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGCGCCCTG TAGCGGCGCA 
     7681 TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA 
     7741 GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT 
     7801 CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC 
     7861 CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT 
     7921 TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA 
     7981 ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG 
     8041 GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT T
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