LOCUS       MR211572                8088 bp    DNA     circular  SYN 
DEFINITION  Vcl (Myc-DDK-tagged) - Mouse vinculin (Vcl)
ACCESSION   NM_009502
VERSION     NM_009502.4
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4243..4334 
                     /label="Myc-DDK"  
     misc_feature    4364..4949 
                     /label="PolyA signal"  
     misc_feature    4421..4440 
                     /label="Restriction Enzyme"  
     misc_feature    5225..5897 
                     /label="ColE1"  
     misc_feature    6327..7121 
                     /label="Kan/Neo"  
     misc_feature    7156..7458 
                     /label="SV40 ori"  
     misc_feature    7544..8021 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4218..4225 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4218 
                     /label="Vcl"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCGGTGTTT CACACGCGTA CGATCGAGAG CATCCTGGAG CCGGTGGCGC 
     1081 AGCAGATCTC GCACCTGGTG ATTATGCACG AGGAGGGCGA GGTGGACGGC AAAGCCATTC 
     1141 CTGACCTCAC CGCGCCCGTA GCCGCCGTGC AGGCGGCCGT CAGCAACCTC GTCCGGGTTG 
     1201 GAAAAGAGAC TGTTCAGACC ACTGAGGATC AGATTCTGAA GAGAGATATG CCACCAGCCT 
     1261 TTATTAAGGT TGAAAATGCT TGCACCAAGC TTGTTCAGGC AGCCCAGATG CTTCAGTCAG 
     1321 ACCCATACTC GGTTCCTGCG CGGGATTACC TCATTGACGG CTCTAGGGGA ATCCTTTCTG 
     1381 GCACATCTGA CCTACTGCTT ACCTTCGATG AGGCTGAGGT TCGTAAAATT ATTAGGGTTT 
     1441 GCAAAGGAAT TTTGGAATAT CTTACAGTGG CAGAGGTAGT GGAAACTATG GAAGACTTGG 
     1501 TCACTTACAC AAAGAATCTT GGGCCAGGAA TGACTAAGAT GGCCAAAATG ATTGATGAGA 
     1561 GACAGCAGGA GTTGACTCAC CAGGAACACC GTGTGATGTT GGTGAACTCA ATGAACACTG 
     1621 TCAAAGAGCT GCTTCCTGTT CTCATTTCAG CTATGAAGAT TTTTGTTACA ACCAAAAACT 
     1681 CAAAAAACCA AGGAATAGAA GAAGCTTTGA AAAATCGAAA TTTTACTGTA GAAAAGATGA 
     1741 GTGCTGAAAT TAACGAGATC ATTCGTGTGT TACAACTCAC TTCCTGGGAT GAAGATGCCT 
     1801 GGGCCAGCAA GGACACTGAA GCCATGAAGA GAGCGCTGGC GTCCATAGAC TCCAAATTGA 
     1861 ACCAGGCCAA AGGTTGGCTC CGTGACCCCA ATGCCTCCCC AGGGGATGCT GGAGAGCAGG 
     1921 CCATCAGGCA GATCTTAGAT GAAGCTGGAA AAGTTGGTGA ACTTTGTGCA GGCAAGGAAC 
     1981 GCAGGGAGAT CCTAGGAACC TGCAAAATGC TAGGGCAGAT GACTGACCAA GTGGCTGACC 
     2041 TCCGAGCCAG AGGACAAGGA GCTTCCCCAG TGGCCATGCA GAAGGCCCAG CAAGTGTCTC 
     2101 AGGGGCTCGA CGTGCTTACC GCCAAAGTGG AGAATGCAGC TCGGAAGCTG GAAGCCATGA 
     2161 CGAACTCAAA GCAGAGCATT GCAAAGAAGA TTGATGCTGC CCAGAATTGG CTGGCGGATC 
     2221 CAAATGGTGG ACCTGAGGGA GAAGAACAGA TTCGAGGGGC TTTGGCTGAA GCTCGGAAGA 
     2281 TTGCAGAATT ATGTGATGAT CCTAAGGAGA GAGATGACAT CCTCCGCTCC CTTGGAGAGA 
     2341 TAGCTGCTCT GACCTCTAAA CTAGGAGACT TGCGAAGACA GGGGAAAGGA GACTCGCCAG 
     2401 AGGCTCGAGC CTTGGCTAAA CAAGTGGCGA CGGCACTACA GAACCTGCAG ACCAAAACCA 
     2461 ACAGGGCCGT GGCCAACAGC AGACCTGCCA AAGCAGCTGT CCACCTCGAG GGCAAGATTG 
     2521 AACAGGCGCA GCGGTGGATT GATAACCCCA CAGTAGATGA CCGTGGAGTC GGTCAGGCTG 
     2581 CCATCCGTGG ACTTGTGGCT GAGGGGCATC GGCTGGCCAA TGTCATGATG GGACCTTATC 
     2641 GCCAAGATCT TCTTGCCAAA TGTGACCGTG TAGACCAGCT AACAGCTCAG CTGGCTGACC 
     2701 TGGCTGCCCG AGGGGAGGGG GAGAGTCCTC AGGCGAGAGC ACTTGCATCC CAGCTTCAGG 
     2761 ACTCCTTAAA GGATCTTAAA GCCCAGATGC AGGAAGCTAT GACTCAAGAG GTATCCGATG 
     2821 TTTTCAGCGA TACTACAACT CCTATCAAGC TGTTGGCAGT AGCCGCCACT GCTCCTCCTG 
     2881 ATGCACCCAA TAGGGAAGAG GTATTTGATG AAAGGGCAGC CAATTTTGAA AACCATTCAG 
     2941 GAAGGCTTGG AGCCACAGCA GAGAAGGCGG CTGCTGTTGG TACTGCCAAT AAATCGACAG 
     3001 TGGAAGGCAT TCAGGCATCT GTGAAGACAG CCCGAGAACT CACTCCCCAG GTCATCTCCG 
     3061 CTGCTCGGAT CTTACTGAGG AACCCTGGTA ACCAGGCTGC TTATGAACAT TTTGAGACCA 
     3121 TGAAGAACCA GTGGATTGAT AATGTTGAAA AAATGACAGG GCTGGTGGAC GAGGCTATTG 
     3181 ATACCAAGTC TCTGTTGGAT GCTTCTGAAG AAGCAATTAA AAAAGACCTG GACAAGTGTA 
     3241 AGGTAGCCAT GGCCAATATT CAGCCTCAGA TGCTGGTCGC TGGAGCAACC AGTATTGCTC 
     3301 GTCGGGCCAA CCGGATTCTG CTGGTTGCTA AGAGGGAGGT AGAGAACTCT GAGGACCCGA 
     3361 AGTTCCGAGA GGCTGTGAAA GCTGCCTCTG ATGAACTGAG CAAAACAATC TCCCCCATGG 
     3421 TGATGGATGC CAAGGCTGTG GCTGGAAACA TCTCTGACCC TGGCCTGCAA AAGAGCTTCC 
     3481 TGGACTCAGG ATATCGGATC CTGGGAGCTG TGGCCAAGGT CAGAGAAGCC TTCCAACCTC 
     3541 AGGAGCCTGA CTTCCCGCCT CCTCCACCAG ACCTTGAACA GCTACGACTA ACTGATGAGC 
     3601 TGGCTCCTCC TAAGCCACCT CTGCCTGAGG GTGAAGTCCC TCCACCCAGG CCCCCACCAC 
     3661 CAGAAGAGAA GGATGAAGAG TTCCCTGAGC AGAAAGCTGG TGAGGTGATT AACCAGCCAA 
     3721 TGATGATGGC CGCCAGGCAG CTCCACGATG AAGCTCGGAA ATGGTCTAGC AAGGGCAATG 
     3781 ACATCATTGC AGCAGCCAAG CGCATGGCTC TGCTGATGGC AGAGATGTCT CGGCTGGTAA 
     3841 GAGGGGGCAG TGGTACCAAG CGGGCACTTA TTCAGTGTGC CAAGGATATC GCCAAGGCCT 
     3901 CTGATGAGGT GACGAGGTTG GCCAAGGAGG TTGCCAAGCA GTGCACAGAT AAGCGGATTA 
     3961 GAACCAATCT CTTACAGGTA TGCGAGCGAA TCCCAACTAT AAGCACCCAG CTCAAAATCC 
     4021 TATCCACAGT GAAGGCCACT ATGCTGGGCC GGACCAACAT CAGTGATGAG GAGTCTGAGC 
     4081 AGGCCACAGA GATGCTGGTT CATAATGCCC AGAACCTCAT GCAGTCTGTG AAGGAGACTG 
     4141 TGCGAGAGGC TGAAGCTGCT TCAATCAAAA TCCGAACAGA TGCTGGCTTT ACTCTGCGCT 
     4201 GGGTCAGAAA GACTCCCTGG TACCAGAGCG GACCGACGCG TACGCGGCCG CTCGAGCAGA 
     4261 AACTCATCTC AGAAGAGGAT CTGGCAGCAA ATGATATCCT GGATTACAAG GATGACGACG 
     4321 ATAAGGTTTA AACGGCCGGC CGCGGTCATA GCTGTTTCCT GAACAGATCC CGGGTGGCAT 
     4381 CCCTGTGACC CCTCCCCAGT GCCTCTCCTG GCCCTGGAAG TTGCCACTCC AGTGCCCACC 
     4441 AGCCTTGTCC TAATAAAATT AAGTTGCATC ATTTTGTCTG ACTAGGTGTC CTTCTATAAT 
     4501 ATTATGGGGT GGAGGGGGGT GGTATGGAGC AAGGGGCAAG TTGGGAAGAC AACCTGTAGG 
     4561 GCCTGCGGGG TCTATTGGGA ACCAAGCTGG AGTGCAGTGG CACAATCTTG GCTCACTGCA 
     4621 ATCTCCGCCT CCTGGGTTCA AGCGATTCTC CTGCCTCAGC CTCCCGAGTT GTTGGGATTC 
     4681 CAGGCATGCA TGACCAGGCT CAGCTAATTT TTGTTTTTTT GGTAGAGACG GGGTTTCACC 
     4741 ATATTGGCCA GGCTGGTCTC CAACTCCTAA TCTCAGGTGA TCTACCCACC TTGGCCTCCC 
     4801 AAATTGCTGG GATTACAGGC GTGAACCACT GCTCCCTTCC CTGTCCTTCT GATTTTAAAA 
     4861 TAACTATACC AGCAGGAGGA CGTCCAGACA CAGCATAGGC TACCTGGCCA TGCCCAACCG 
     4921 GTGGGACATT TGAGTTGCTT GCTTGGCACT GTCCTCTCAT GCGTTGGGTC CACTCAGTAG 
     4981 ATGCCTGTTG AATTGGGTAC GCGGCCAGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT 
     5041 AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA 
     5101 GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC 
     5161 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT 
     5221 ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT 
     5281 GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG 
     5341 CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA 
     5401 CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA 
     5461 CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC 
     5521 GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG 
     5581 AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG 
     5641 TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA 
     5701 GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC 
     5761 TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG 
     5821 GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA 
     5881 TGAGTAACCT GAGGCTATGG CAGGGCCTGC CGCCCCGACG TTGGCTGCGA GCCCTGGGCC 
     5941 TTCACCCGAA CTTGGGGGGT GGGGTGGGGA AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA 
     6001 ATGGGGTCTC GGTGGGGTAT CGACAGAGTG CCAGCCCTGG GACCGAACCC CGCGTTTATG 
     6061 AACAAACGAC CCAACACCGT GCGTTTTATT CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT 
     6121 TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA 
     6181 CTCCAGCATG AGATCCCCGC GCTGGAGGAT CATCCAGCCG GCGTCCCGGA AAACGATTCC 
     6241 GAAGCCCAAC CTTTCATAGA AGGCGGCGGT GGAATCGAAA TCTCGTGATG GCAGGTTGGG 
     6301 CGTCGCTTGG TCGGTCATTT CGAACCCCAG AGTCCCGCTC AGAAGAACTC GTCAAGAAGG 
     6361 CGATAGAAGG CGATGCGCTG CGAATCGGGA GCGGCGATAC CGTAAAGCAC GAGGAAGCGG 
     6421 TCAGCCCATT CGCCGCCAAG CTCTTCAGCA ATATCACGGG TAGCCAACGC TATGTCCTGA 
     6481 TAGCGATCCG CCACACCCAG CCGGCCACAG TCGATGAATC CAGAAAAGCG GCCATTTTCC 
     6541 ACCATGATAT TCGGCAAGCA GGCATCGCCA TGGGTCACGA CGAGATCCTC GCCGTCGGGC 
     6601 ATGCTCGCCT TGAGCCTGGC GAACAGTTCG GCTGGCGCGA GCCCCTGATG CTCTTCGTCC 
     6661 AGATCATCCT GATCGACAAG ACCGGCTTCC ATCCGAGTAC GTGCTCGCTC GATGCGATGT 
     6721 TTCGCTTGGT GGTCGAATGG GCAGGTAGCC GGATCAAGCG TATGCAGCCG CCGCATTGCA 
     6781 TCAGCCATGA TGGATACTTT CTCGGCAGGA GCAAGGTGAG ATGACAGGAG ATCCTGCCCC 
     6841 GGCACTTCGC CCAATAGCAG CCAGTCCCTT CCCGCTTCAG TGACAACGTC GAGCACAGCT 
     6901 GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC GATAGCCGCG CTGCCTCGTC TTGCAGTTCA 
     6961 TTCAGGGCAC CGGACAGGTC GGTCTTGACA AAAAGAACCG GGCGCCCCTG CGCTGACAGC 
     7021 CGGAACACGG CGGCATCAGA GCAGCCGATT GTCTGTTGTG CCCAGTCATA GCCGAATAGC 
     7081 CTCTCCACCC AAGCGGCCGG AGAACCTGCG TGCAATCCAT CTTGTTCAAT CATGCGAAAC 
     7141 GATCCTCATC CTGTCTCTTG ATCGATCTTT GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC 
     7201 TCACTACTTC TGGAATAGCT CAGAGGCCGA GGCGGCCTCG GCCTCTGCAT AAATAAAAAA 
     7261 AATTAGTCAG CCATGGGGCG GAGAATGGGC GGAACTGGGC GGAGTTAGGG GCGGGATGGG 
     7321 CGGAGTTAGG GGCGGGACTA TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     7381 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCTGGTTGCT GACTAATTGA GATGCATGCT 
     7441 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCCTAACT GACACACATT 
     7501 CCACAGCTGG TTCTTTCCGC CTCAGGACTC TTCCTTTTTC AATATTATTG AAGCATTTAT 
     7561 CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA 
     7621 GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG CGCCCTGTAG CGGCGCATTA 
     7681 AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG 
     7741 CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA 
     7801 GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC 
     7861 AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT 
     7921 CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA 
     7981 ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC 
     8041 TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTT
//