LOCUS       MR211571                8079 bp    DNA     circular  SYN 
DEFINITION  Rnf31 (Myc-DDK-tagged) - Mouse ring finger protein 31 (Rnf31)
ACCESSION   NM_194346
VERSION     NM_194346.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4236..4327 
                     /label="Myc-DDK"  
     misc_feature    4357..4942 
                     /label="PolyA signal"  
     misc_feature    4414..4433 
                     /label="Restriction Enzyme"  
     misc_feature    5218..5890 
                     /label="ColE1"  
     misc_feature    6320..7114 
                     /label="Kan/Neo"  
     misc_feature    7149..7451 
                     /label="SV40 ori"  
     misc_feature    7537..8014 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4218..4224 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4218 
                     /label="Rnf31"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCGGGAGAC GAGGAGCGAG GCTTCCTGGC GGCCCGCGAG GAGCTGGCGA 
     1081 GCGCCCTGAG GTGGGATTCT GCGCAGGTTT TTCCCCTGGA GCAGCTCATG CCGCTTCTGG 
     1141 CCACCTCTCT GCCACCAGCC GCCCGCTACC TGCAGCTGGA CGCCGGACGC TTGGTCCGCT 
     1201 GCAACGCTCA TGGGGAGCCT CGAAACTACC TCAACACCCT ATCCACGGCC CTGAACATCC 
     1261 TGGAAAAATA TGGTCGCAAC CTCCTCAGCC CGCAGCGGCC CCGGTATTGG CGCTCAGTGA 
     1321 AGTTTAATAA CCCCGTCTTT CGCAGCACGG TGGATGCTGT GCAGGGTGGC CGGGATGTAC 
     1381 TACGGTTGTA TGGCTATACT GAGGAGCGCC CAGATGGATT GAGTTTCCCC GAAGGGCAGG 
     1441 AGGAACCAGA TGAATACCAG GTTGCTGTTG TCACACTAGA AGTACTGCTG CTTCGCACCG 
     1501 AGCTCAGTTT GCTGTTGCAG AATACTCATC CCAGACAGAA TGCACTGGAC CAGCTGCTAA 
     1561 GAGAGAGCGT TGAAGATGGT ATGCTGCAGC TTTCAGAGTT TCACCCCCTT CTGAGGGAGA 
     1621 TTGTTCCTGG CCCCCGCCCC TCTGCCCAAG GCTCCACTCC TGGTCCCTGT TTCCTCTGTG 
     1681 GTTCTGCCCC AGGCACACTG CACTGTCCAG CCTGTAACCA AGTCTCGTGC CCAGCTTGTG 
     1741 ACATTTTGTT CCATGGGCAT CCGTCCCGTG CACATCACCT TCGCCAAGCC CTGCCTGGGT 
     1801 CCCACCAGAC TGCCAGCCTG AGCTCTAGTT TACCTGCCTC GTCCCAACCA CGGCCCCCCT 
     1861 CCTCCTCCTT GGCCCTGGGA GATAGCTCTC TTTCTTCCCC TGACCCTGCA AATGCCTGTC 
     1921 TGCCCTGGCA TTGTCTTACC TGTGCCACAC TAAATGAGCC TTGGGCAGTG TTCTGTGCAG 
     1981 TCTGTAGTCA GCCCAAAGGC TGCAAGGTGC CGGGAATAGA GGGTTCCCAT GGAACCGGGG 
     2041 GCCTAGAACC TGAGCCTGCA CGGGATCAAT GGGCCTGCCA GAGCTGTACC TTTGAGAATG 
     2101 AGGCAGCAGC TGTGCTATGC GCCATATGTG AGCGACCTCG GCTGGCCCAG CCTCCCAGCT 
     2161 TGGTGGTGGA TTCCCATGAT GCTGGTGTTT GCCAACAGTC CCTTAAGCAG GAGGATCCTT 
     2221 TGCTCACCGC TGCCCAGCCT CAGGTGTGGT ACTGTGACCA TTGTACCTTC TGCAATTCAG 
     2281 GCCCTGTCTG GGTGTGTGCC ATGTGCAACC GAACCCGAGA CCCCATCCCT ACACAGCCTG 
     2341 CCCTCCAGTC CTATCCCAGC TCTTTGGAAA AGGGACGCCC AAAGCCAGGG TCCTCACAAC 
     2401 ACCTTGGTTC CTCCCTGCCT GCTTCCTGTG GAGACCCAGA GAAACAACGC CAAGATAAGA 
     2461 TGCGGAAGGA AGGTCTCCAG CTCGTGAGCA TGATCCAGGA AGGAGAAACT GCGGGTGCCA 
     2521 GTCCAGAAGA GGTCTTCTCA GCTCTCCAAT ACTCAGGCAC AGAGGTGCCC CTCCAGTGGT 
     2581 TGCGTTCAGA GCTGTCCTAC GTCCTGGAGA TGGTGGCTGA GCTTGCTGGA CAACAGGATC 
     2641 CAGAGCTGGG GGCCTTTTCC TGTCAGGAAG CCCGGAAAGC CTGGCTTGAT CGCCATGGCA 
     2701 ACCTGGATGA AGCTGTAGAG GAGTGTGTGA GGGCCAGGAG GAGGAAGGTG CACGAGCTGC 
     2761 AGTCCCTGGG CTTTGGGCCT AAGGAAGGGT CACTACAGGC ATTGTTCCAG CATGGGGGTG 
     2821 ACGTGGCTCG GGCCCTGACT GAGTTACAGC GCCAGCGCCT GGAGCCCTTC CATCAGCGCC 
     2881 TATGGGACAG AGACCCTGAA CCCACTCCCT GCTGGGATGG GCTGGACAGA CAGAGCTTGG 
     2941 TCAGACGCCT TCTGGCCGTC TACACACTCC CCAGCTGGGG CCGAGCAGAG CTGGCGCTGG 
     3001 CGCTGCTGCA GGAGACACCC AGGAACTATG AGTTGTTGGA CGTGGTGGAG GCTGTGAGGC 
     3061 ACAGCCAGGA CCGGGCCTTT CTGCGTCGAC TGCTTGCCCA GGAATGTGCT GTGTGCGGGT 
     3121 GGGCCCTTCC CCGCAACCGG ATGCAGGCCC TGATCTCCTG TGAGTGCACC ATATGTCCCG 
     3181 AATGCTTCCG CCAACACTTC ACCATTGCCC TGAAGGAGAA GCACATCACA GACATGGTGT 
     3241 GCCCTGCCTG TGGCCGCCCT GACCTCACTG ATGACGCTCA GTTACTCAGC TACTTCTCCA 
     3301 CCCTTGACAT CCAGCTCAGA GAGAGCCTAG ACCCCGATGC ATATGCCCTG TTTCACAAGA 
     3361 AGCTGACCGA GGCTGTGCTT ATGCGAGACC CCAAGTTCTT GTGGTGCGCC CAGTGTTCCT 
     3421 TTGGCTTCAT CTATGAACGC GAACAGCTGG AGGCGACGTG TCCCCAGTGT CACCAGACCT 
     3481 TCTGTGTGCG CTGCAAGCGC CAGTGGGAGG AGCAGCACAG AGGACGGAGT TGTGAGGATT 
     3541 TCCAGAACTG GAAACGCACC AATGACCCAG AGTACCAGGC TCAAGGCTTG GCCATGTACC 
     3601 TTCAGGAAAA CGGCATTGAC TGTCCGAAAT GCAAGTTCTC GTACGCACTG GCCCGGGGAG 
     3661 GCTGCATGCA CTTCCACTGC ACGCAGTGTC GACACCAGTT CTGCAGTGGC TGCTACAACG 
     3721 CCTTTTACGC CAAGAATAAA TGTCCAGACC CTAACTGCAA GGTGAAAAAG TCCCTGCATG 
     3781 GCCACCACCC TCGAGACTGC CTCTTCTACC TACGGGACTG GACTGCTGCC CGCCTCCAGA 
     3841 AACTGTTGCA GGACAATAAT GTCATGTTTA ATACAGAGCC TCCAGCTGGG ACACGGGCAG 
     3901 TCCCTGGAGG GGGCTGCAGA GTGATGGAGC AGAAGGAGGT CCATAGTGGG TTCAGGGATG 
     3961 AAGCTTGCGG CAAGGAAACT CCACCTGGCT ATGCCGGCCT ATGTCAGGCA CACTACAAAG 
     4021 AGTATCTCGT GAGCCTCATC AATGCCCATT CACTGGACCC AGCTACCTTG TATGAAGTGG 
     4081 AGGAGCTGGA GACAGCCACT ATTCGCTACC TACATTTAGC TCCTCAGCCC GCGGATGGAG 
     4141 AGGATCTGCC TGCTTACCAG GCCCGGCTAT TACAGAAGCT GAGAGAAGAG GTACCCTTGG 
     4201 GACAGAGTAT TGCCCGCAGA AGAAAGACGC GTACGCGGCC GCTCGAGCAG AAACTCATCT 
     4261 CAGAAGAGGA TCTGGCAGCA AATGATATCC TGGATTACAA GGATGACGAC GATAAGGTTT 
     4321 AAACGGCCGG CCGCGGTCAT AGCTGTTTCC TGAACAGATC CCGGGTGGCA TCCCTGTGAC 
     4381 CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA GTTGCCACTC CAGTGCCCAC CAGCCTTGTC 
     4441 CTAATAAAAT TAAGTTGCAT CATTTTGTCT GACTAGGTGT CCTTCTATAA TATTATGGGG 
     4501 TGGAGGGGGG TGGTATGGAG CAAGGGGCAA GTTGGGAAGA CAACCTGTAG GGCCTGCGGG 
     4561 GTCTATTGGG AACCAAGCTG GAGTGCAGTG GCACAATCTT GGCTCACTGC AATCTCCGCC 
     4621 TCCTGGGTTC AAGCGATTCT CCTGCCTCAG CCTCCCGAGT TGTTGGGATT CCAGGCATGC 
     4681 ATGACCAGGC TCAGCTAATT TTTGTTTTTT TGGTAGAGAC GGGGTTTCAC CATATTGGCC 
     4741 AGGCTGGTCT CCAACTCCTA ATCTCAGGTG ATCTACCCAC CTTGGCCTCC CAAATTGCTG 
     4801 GGATTACAGG CGTGAACCAC TGCTCCCTTC CCTGTCCTTC TGATTTTAAA ATAACTATAC 
     4861 CAGCAGGAGG ACGTCCAGAC ACAGCATAGG CTACCTGGCC ATGCCCAACC GGTGGGACAT 
     4921 TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA TGCGTTGGGT CCACTCAGTA GATGCCTGTT 
     4981 GAATTGGGTA CGCGGCCAGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT 
     5041 ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC 
     5101 CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA 
     5161 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA 
     5221 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC 
     5281 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG 
     5341 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC 
     5401 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG 
     5461 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT 
     5521 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT 
     5581 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG 
     5641 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC 
     5701 GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA 
     5761 GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC 
     5821 CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAACC 
     5881 TGAGGCTATG GCAGGGCCTG CCGCCCCGAC GTTGGCTGCG AGCCCTGGGC CTTCACCCGA 
     5941 ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA AACGCGGGCG TATTGGCCCC AATGGGGTCT 
     6001 CGGTGGGGTA TCGACAGAGT GCCAGCCCTG GGACCGAACC CCGCGTTTAT GAACAAACGA 
     6061 CCCAACACCG TGCGTTTTAT TCTGTCTTTT TATTGCCGTC ATAGCGCGGG TTCCTTCCGG 
     6121 TATTGTCTCC TTCCGTGTTT CAGTTAGCCT CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT 
     6181 GAGATCCCCG CGCTGGAGGA TCATCCAGCC GGCGTCCCGG AAAACGATTC CGAAGCCCAA 
     6241 CCTTTCATAG AAGGCGGCGG TGGAATCGAA ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG 
     6301 GTCGGTCATT TCGAACCCCA GAGTCCCGCT CAGAAGAACT CGTCAAGAAG GCGATAGAAG 
     6361 GCGATGCGCT GCGAATCGGG AGCGGCGATA CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT 
     6421 TCGCCGCCAA GCTCTTCAGC AATATCACGG GTAGCCAACG CTATGTCCTG ATAGCGATCC 
     6481 GCCACACCCA GCCGGCCACA GTCGATGAAT CCAGAAAAGC GGCCATTTTC CACCATGATA 
     6541 TTCGGCAAGC AGGCATCGCC ATGGGTCACG ACGAGATCCT CGCCGTCGGG CATGCTCGCC 
     6601 TTGAGCCTGG CGAACAGTTC GGCTGGCGCG AGCCCCTGAT GCTCTTCGTC CAGATCATCC 
     6661 TGATCGACAA GACCGGCTTC CATCCGAGTA CGTGCTCGCT CGATGCGATG TTTCGCTTGG 
     6721 TGGTCGAATG GGCAGGTAGC CGGATCAAGC GTATGCAGCC GCCGCATTGC ATCAGCCATG 
     6781 ATGGATACTT TCTCGGCAGG AGCAAGGTGA GATGACAGGA GATCCTGCCC CGGCACTTCG 
     6841 CCCAATAGCA GCCAGTCCCT TCCCGCTTCA GTGACAACGT CGAGCACAGC TGCGCAAGGA 
     6901 ACGCCCGTCG TGGCCAGCCA CGATAGCCGC GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA 
     6961 CCGGACAGGT CGGTCTTGAC AAAAAGAACC GGGCGCCCCT GCGCTGACAG CCGGAACACG 
     7021 GCGGCATCAG AGCAGCCGAT TGTCTGTTGT GCCCAGTCAT AGCCGAATAG CCTCTCCACC 
     7081 CAAGCGGCCG GAGAACCTGC GTGCAATCCA TCTTGTTCAA TCATGCGAAA CGATCCTCAT 
     7141 CCTGTCTCTT GATCGATCTT TGCAAAAGCC TAGGCCTCCA AAAAAGCCTC CTCACTACTT 
     7201 CTGGAATAGC TCAGAGGCCG AGGCGGCCTC GGCCTCTGCA TAAATAAAAA AAATTAGTCA 
     7261 GCCATGGGGC GGAGAATGGG CGGAACTGGG CGGAGTTAGG GGCGGGATGG GCGGAGTTAG 
     7321 GGGCGGGACT ATGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     7381 GGAGCCTGGG GACTTTCCAC ACCTGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     7441 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCCTAAC TGACACACAT TCCACAGCTG 
     7501 GTTCTTTCCG CCTCAGGACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT 
     7561 TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG 
     7621 CGCACATTTC CCCGAAAAGT GCCACCTGAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG 
     7681 GGTGTGGTGG TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT 
     7741 TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT 
     7801 CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT 
     7861 GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG 
     7921 ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC 
     7981 CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA 
     8041 AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTTT
//