LOCUS       MR211558                8055 bp    DNA     circular  SYN 
DEFINITION  Gemin4 (Myc-DDK-tagged) - Mouse gem (nuclear organelle) associated protein 4 (Gemin4)
ACCESSION   NM_177367
VERSION     NM_177367.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4212..4303 
                     /label="Myc-DDK"  
     misc_feature    4333..4918 
                     /label="PolyA signal"  
     misc_feature    4390..4409 
                     /label="Restriction Enzyme"  
     misc_feature    5194..5866 
                     /label="ColE1"  
     misc_feature    6296..7090 
                     /label="Kan/Neo"  
     misc_feature    7125..7427 
                     /label="SV40 ori"  
     misc_feature    7513..7990 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4194..4200 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4194 
                     /label="Gemin4"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGACCTAGGA CCCTTGAATA TCTGTGAGGA GATGACTATT CTGCATGGGG 
     1081 GCTTCTTGAT GGCTGAGCAG CTGTTCCACC CCAAAGCCCT GGTACAGTTG ACCAAGTCTG 
     1141 ATTGGGAACA CGTGGGACAG CCCATTGTGG AGGCCTTGAA GGAGATCTCC TCAGCCACAG 
     1201 CACATTCCCA GCCCTTTGCC TGGAAGAAGA AAGCACTGAT CATCATCTGG ACCAAGGTTC 
     1261 TTCAGCCCTC CCCTGTCAGC CCTTCTGACA CTGATACTCG GTGGCAGGAA GATGTATTCT 
     1321 TCTCCGTGGG CAACATGATC CCTACCATCA ACCACACAGT CCTTTTTGAG CTGCTCAAGT 
     1381 CTCTGGAAGC TTCTGGACTC TTCATTCAGC TGCTAATGGC CTTGCCTACC ACCATTGGCC 
     1441 GCTCAGAACT ACAGAGCTTC CTGGAGCACA TGACTGTTGA CACATCTTCC AAGGATGTGG 
     1501 CCTTCTTCTT AGATGTCTGG TGGGAAATGA TGAAGCACAA GGGCGACCAG CAGGACCCCT 
     1561 TGCTTTCCCA GTTCAGGACG ATGGCCCATA AATACTTGCC ATCCTCAGAT GAGTTTTCCC 
     1621 ATCCTCCAAA GAGGTTTAAG TCTGACCCAG ATGTGGGTCC CACCATGCCT CTTTTGGCCA 
     1681 TGTTGCTTAA CGGGCTGAAG CAGATCCAAA AGAGAATCCT GTCCCTTGGA ATGAAGTGCT 
     1741 GTGCCTTAGC TAACTTGGCT GACATGCTGA GTGTGTTTGC ACTTCTGGAG GATGACACCC 
     1801 AGGAGGTGTC GGCCACCGTG TACCTAGACA AACTAGCTAC AGTGATCTCT GTGTGGAACT 
     1861 CGGACACCCA GAACCCATAC CATCGACAGG CCCTGGCAGA CAAGGTAAAG GAGGCAGAGC 
     1921 GAGATGTGAG TCTCACCTCA CTGGCCAAAC TCCCCAGCGA GACTGTTTTC ATGGGGTTTG 
     1981 AGCTTATGTA CAACCTGCTG CAAGAGTGGG GTGCAGAACT GCAGGACATG CTCAACAGCA 
     2041 GTCAGGGAAC AAATTATGAC ACTTACCGGC TGTGTGACAG CCTGACTTCC TTCAGCCAGA 
     2101 ACTTGGAGCT CTACCTGGAT ACTACCAACA TGCCCAAGGA GGAGAGGGAG GTGGTCTCAG 
     2161 AATTGGCTGA GTGTGTCAGG GACTTCCTGA GGAAAACCAG CAGAATGCTG AATGACAAGG 
     2221 GCCTTGAGGA CATCACTGCC TCCATTGCCA TGGCCATCAT TGAGCAGAAG ATGGACCGGC 
     2281 ACATGGAAAT GTGCTACATT TTTGCTTCTG AGAAGAAGTG GGCCTTTTCA GACGAGTGGG 
     2341 TTGCCTGCCT GGTTAACAAC AGGTCTCTCT TCAGAGAACC AGACTTGGTC TTGAGTCTGC 
     2401 TAGAAACAGT GATGGAAGTC ATCAGCTCTG ATCGGGTTAT CCCCCAGCCT CAGATCAAGC 
     2461 AGGTAATCTG CTTGACCCTG GAATGCTATG AAGACCTCTC TCTTCCAGAC AAAAATAAAG 
     2521 TTCTTTCTGG TGTCCTGCAT TATTTGGGGC CAAAGGGTCT CTCAGACAGG TTGTCTGATT 
     2581 ACTTGGAGGG TTTTCAGGAA GACCTCAACA CGACGTTTAA CCAGCTCGCA CAGAGTACCT 
     2641 CTGAACAGGG CCTGGCTAAA GCTGTGGCCT CCGTGGCCCA CCTGGTGATC CTGAACCCAG 
     2701 AAGTTGTGGT GAAGAAGATG TGTAGCCTAG CTGTGACCAA TCTTGGCACA CATAGGTTCT 
     2761 TGGCTCAGAT TCTCACTGCC TTCCCTGCTC TGAAGTTCAC GGAAGAACAA GGGGCAAATC 
     2821 CATGTACCAG TTTCATGGTG TCTTGCCTCA AAGAAACTGT CTGGACCAAG TTCTCCACAC 
     2881 CCAAAGAAGA GAAGCAGTTT CTGGAGCTGG TAAGCTGCCT GACGAATCCT GTGAAGCCAC 
     2941 AGGGGATTCC AGTGGCTGCT CTTCTGGAGC CAGATGAGGT GCTCAAGGAG TTTGTCCTGC 
     3001 CTTTCTTGAT GTTGGACATG GAGGAGGTGG GCCTGAGTCT GAAGCTCTTC ATCCAGACCC 
     3061 TCGAAGCCAA TGCAGGCCTG GAAGAGTATT GGCTGCAGAC CTGCTCCCCA TTCCCTCTGC 
     3121 TCTTCAGCCT GTGCCAGCTC CTGGACAGCT TCAGCAAGTT CTGGCAGCTC CCCCGGGAGA 
     3181 AGCGCTACCT CACGCTGGAC AGCAAGGATC TTGTGACCCA CATTCTGGAA CTCCTCTGTG 
     3241 AGATCGTGCT AGCCAACACA GAAACCTTCT CCCCAGACAC CTGGGCCAAG TCCCTGTCCT 
     3301 GGCTCCACCG CAAATTAGAC CAGCTAGATT GGACTGTGGG CCTGAGACTA AAGAACTTCT 
     3361 TTGAAGGCCA CTTCAAGTGT GAGGTGCCAG CTACCCTTTT TGATATCTGT AAGCTCTCTG 
     3421 AGGCCCAGTG GACCTCACAG GTCCACGAAG GGTATGGGCC TGGCACGGGC CTTCTCGCCT 
     3481 GGATGGAGTG CTGTTCAGTC TCCAGCAGTA TCTCTGACCA GATGCTTTCC CTTCTGGTGA 
     3541 TGGATGTGGG CAATCCTGAG GAGGTCAGAC TGTTTAGCAA GGGCTTTCTG GTGGCCCTGG 
     3601 TACAAGTCAT GCCTTGGTGT AGCCCCCAGG AATGGCAGTA CCTTCAGCAG CTGACCAGGA 
     3661 AGCTATTGGA GAAACAGCTT CTTCATGTCC CTTACAGTCT AGAGTATATT CAGTTTGTTC 
     3721 CCCTGCTCAA CCTGAAGCCT TTTGCCCAGG AACTTCAGCT CTCTGTTCTT TCTTTGAGAG 
     3781 TGTTCCAGTT TCTCTGCAGC CAGAGCTGTT GTAACTGGCT TCCTTTGGAC GGTTGGAGCC 
     3841 ATGTGGTCAA ACTCCTCTGT AACAGTCTGA CCAGTGTCCT GGATGCAGTC AGGTTGATGC 
     3901 AGTCGGTGGG TCCCTGGGCC CAAGGACAAG AAGGGGATCT TACTCAGGAA ACCCTGTTTG 
     3961 TCTATACCCA GATGTTCTGT CATGTTCTGC ATATCATGGC CATGCTCCCT CAGGAGGTCT 
     4021 GTGAACCCCT CTACGTGCTG GCCTTGGAAA TCCTTACCTG CTACGAGACT CTAAGCAAAA 
     4081 GCAACCCTTC TGTCAGCTCC TTGCTCCAGA AGGTAGACGA GCAGCGCTTC TTAAAGTCTA 
     4141 TCGCCAAGAA CATTAGCCCT GAGGAGCGGC GCCAAACCCT GCTACAAAAG ATCAACAACT 
     4201 TCACGCGTAC GCGGCCGCTC GAGCAGAAAC TCATCTCAGA AGAGGATCTG GCAGCAAATG 
     4261 ATATCCTGGA TTACAAGGAT GACGACGATA AGGTTTAAAC GGCCGGCCGC GGTCATAGCT 
     4321 GTTTCCTGAA CAGATCCCGG GTGGCATCCC TGTGACCCCT CCCCAGTGCC TCTCCTGGCC 
     4381 CTGGAAGTTG CCACTCCAGT GCCCACCAGC CTTGTCCTAA TAAAATTAAG TTGCATCATT 
     4441 TTGTCTGACT AGGTGTCCTT CTATAATATT ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG 
     4501 GGGCAAGTTG GGAAGACAAC CTGTAGGGCC TGCGGGGTCT ATTGGGAACC AAGCTGGAGT 
     4561 GCAGTGGCAC AATCTTGGCT CACTGCAATC TCCGCCTCCT GGGTTCAAGC GATTCTCCTG 
     4621 CCTCAGCCTC CCGAGTTGTT GGGATTCCAG GCATGCATGA CCAGGCTCAG CTAATTTTTG 
     4681 TTTTTTTGGT AGAGACGGGG TTTCACCATA TTGGCCAGGC TGGTCTCCAA CTCCTAATCT 
     4741 CAGGTGATCT ACCCACCTTG GCCTCCCAAA TTGCTGGGAT TACAGGCGTG AACCACTGCT 
     4801 CCCTTCCCTG TCCTTCTGAT TTTAAAATAA CTATACCAGC AGGAGGACGT CCAGACACAG 
     4861 CATAGGCTAC CTGGCCATGC CCAACCGGTG GGACATTTGA GTTGCTTGCT TGGCACTGTC 
     4921 CTCTCATGCG TTGGGTCCAC TCAGTAGATG CCTGTTGAAT TGGGTACGCG GCCAGCGGCG 
     4981 AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC 
     5041 AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT 
     5101 GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG 
     5161 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC 
     5221 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC 
     5281 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT 
     5341 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT 
     5401 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC 
     5461 AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA 
     5521 GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA 
     5581 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG 
     5641 TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA 
     5701 AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG 
     5761 GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG 
     5821 AAGTTTTAAA TCAATCTAAA GTATATATGA GTAACCTGAG GCTATGGCAG GGCCTGCCGC 
     5881 CCCGACGTTG GCTGCGAGCC CTGGGCCTTC ACCCGAACTT GGGGGGTGGG GTGGGGAAAA 
     5941 GGAAGAAACG CGGGCGTATT GGCCCCAATG GGGTCTCGGT GGGGTATCGA CAGAGTGCCA 
     6001 GCCCTGGGAC CGAACCCCGC GTTTATGAAC AAACGACCCA ACACCGTGCG TTTTATTCTG 
     6061 TCTTTTTATT GCCGTCATAG CGCGGGTTCC TTCCGGTATT GTCTCCTTCC GTGTTTCAGT 
     6121 TAGCCTCCCC CTAGGGTGGG CGAAGAACTC CAGCATGAGA TCCCCGCGCT GGAGGATCAT 
     6181 CCAGCCGGCG TCCCGGAAAA CGATTCCGAA GCCCAACCTT TCATAGAAGG CGGCGGTGGA 
     6241 ATCGAAATCT CGTGATGGCA GGTTGGGCGT CGCTTGGTCG GTCATTTCGA ACCCCAGAGT 
     6301 CCCGCTCAGA AGAACTCGTC AAGAAGGCGA TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG 
     6361 GCGATACCGT AAAGCACGAG GAAGCGGTCA GCCCATTCGC CGCCAAGCTC TTCAGCAATA 
     6421 TCACGGGTAG CCAACGCTAT GTCCTGATAG CGATCCGCCA CACCCAGCCG GCCACAGTCG 
     6481 ATGAATCCAG AAAAGCGGCC ATTTTCCACC ATGATATTCG GCAAGCAGGC ATCGCCATGG 
     6541 GTCACGACGA GATCCTCGCC GTCGGGCATG CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT 
     6601 GGCGCGAGCC CCTGATGCTC TTCGTCCAGA TCATCCTGAT CGACAAGACC GGCTTCCATC 
     6661 CGAGTACGTG CTCGCTCGAT GCGATGTTTC GCTTGGTGGT CGAATGGGCA GGTAGCCGGA 
     6721 TCAAGCGTAT GCAGCCGCCG CATTGCATCA GCCATGATGG ATACTTTCTC GGCAGGAGCA 
     6781 AGGTGAGATG ACAGGAGATC CTGCCCCGGC ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC 
     6841 GCTTCAGTGA CAACGTCGAG CACAGCTGCG CAAGGAACGC CCGTCGTGGC CAGCCACGAT 
     6901 AGCCGCGCTG CCTCGTCTTG CAGTTCATTC AGGGCACCGG ACAGGTCGGT CTTGACAAAA 
     6961 AGAACCGGGC GCCCCTGCGC TGACAGCCGG AACACGGCGG CATCAGAGCA GCCGATTGTC 
     7021 TGTTGTGCCC AGTCATAGCC GAATAGCCTC TCCACCCAAG CGGCCGGAGA ACCTGCGTGC 
     7081 AATCCATCTT GTTCAATCAT GCGAAACGAT CCTCATCCTG TCTCTTGATC GATCTTTGCA 
     7141 AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA CTACTTCTGG AATAGCTCAG AGGCCGAGGC 
     7201 GGCCTCGGCC TCTGCATAAA TAAAAAAAAT TAGTCAGCCA TGGGGCGGAG AATGGGCGGA 
     7261 ACTGGGCGGA GTTAGGGGCG GGATGGGCGG AGTTAGGGGC GGGACTATGG TTGCTGACTA 
     7321 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCT 
     7381 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     7441 CTTTCCACAC CCTAACTGAC ACACATTCCA CAGCTGGTTC TTTCCGCCTC AGGACTCTTC 
     7501 CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT 
     7561 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA 
     7621 CCTGACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG 
     7681 ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC 
     7741 GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GGCTCCCTTT AGGGTTCCGA 
     7801 TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT AGGGTGATGG TTCACGTAGT 
     7861 GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC GTTCTTTAAT 
     7921 AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGTCTA TTCTTTTGAT 
     7981 TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA 
     8041 TTTAACGCGA ATTTT
//