LOCUS       MR211543                8040 bp    DNA     circular  SYN 
DEFINITION  Uba6 (Myc-DDK-tagged) - Mouse ubiquitin-like modifier activating enzyme 6 (Uba6)
ACCESSION   NM_172712
VERSION     NM_172712.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4197..4288 
                     /label="Myc-DDK"  
     misc_feature    4318..4903 
                     /label="PolyA signal"  
     misc_feature    4375..4394 
                     /label="Restriction Enzyme"  
     misc_feature    5179..5851 
                     /label="ColE1"  
     misc_feature    6281..7075 
                     /label="Kan/Neo"  
     misc_feature    7110..7412 
                     /label="SV40 ori"  
     misc_feature    7498..7975 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4179..4185 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4179 
                     /label="Uba6"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGCGGTCC GAGCCGTTGG CTGTGCTTTC CTGTGAAGAG GCGTCCTGCT 
     1081 CCTCCTGGGG AGCCTGCGGT GCAAGTAAAA ATTTACCTAC CATGACAACA GAATCTTTGG 
     1141 AAATCGATGA TGGATTATAC AGTCGCCAGA GATATGTTCT TGGTGACACA GCAATGCAGA 
     1201 AGATGGCCAA GTCCTGTGTC TTCTTAAGTG GTATGGGTGG TCTTGGAGTG GAAATTGCAA 
     1261 AGAATCTTGT TCTTGCAGGG ATTAAGGCCC TAACAATTCA TGATACAAAA AAATGCCAAG 
     1321 CATGGGATCT AGGGACCAAT TTCTTCCTGT GTGAAGATGA TGTTGTTAAT GAGAGAAACA 
     1381 GGGCTGAAGC TGTACTTCAT CGTATTGCAG AACTAAATCC ATATGTTCAG GTCTCATCAT 
     1441 CCTCTGCCCC TCTTGATGAA ACCACAGACC TCTCTTTCTT AGAAAAATAT CAGTGTGTAG 
     1501 TATTGACTGA AATAAAACTG ACATTACAAA AGAAGATCAA CAATTTTTGC CATTCTCATT 
     1561 GCCCTCCAAT TAAGTTCATT AGTGCAGATG TACATGGAAT TTGGTCCAGG TTGTTTTGTG 
     1621 ATTTTGGTGA TGAATTTGAA GTTTCAGATA CAACAGGAGA AGAACCAAAA GAAATTTTCA 
     1681 TTTCAAACAT AACGCAAGCT AATCCAGGTA TTGTCACTTG CCTTGAAAGT CATCCTCACA 
     1741 AGCTTGAGAC AGGACAGTTC CTAACATTTC GAGAAATTCA TGGAATGACA GGCTTAAATG 
     1801 GATCTGTACA ACAGATAACT GTCATATCAC CATTTTCCTT CAGCATTGGT GATACTACAA 
     1861 AACTGGACCC ATATTTACAT GGAGGCATAG CCGTGCAGGT GAAGACTCCT AAAACATTCT 
     1921 GCTTTGAACC CCTGGAGAGC CAGATAAAAC ATCCAAGGTG CCTTATTGCA GATTTTAGCA 
     1981 AACCTGAGGC ACCTTTAGAG ATTCATCTCG CTATGCTTGC CTTGGACCAG TTTCAGGAGA 
     2041 ACTATAACCG TAAGCCAAAT ATCAGATGTC AGCAGGATTC AGATGAACTA TTGAAGCTAA 
     2101 CAGTATCTAT AAATGAAACC TTGGAAGAGA AGCCTGAAGT GAATGCTGAC ATTGTGCACT 
     2161 GGCTTTCGTG GACTGCCCAA GGCTTTTTAC CACCACTCGC TGCTGCAGTT GGAGGTGTTG 
     2221 CTAGCCAAGA AGTCTTAAAA GCTGTGACGG GGAAGTTCTC GCCCTTGTGT CAATGGTTGT 
     2281 ACCTTGAAGC AGCAGATACT GTGGAATCCC TAGGCAATCC TGGGCATGAA GAGTTTCTCC 
     2341 CACGAGGAGA TAGATATGAT GCCATACGTG CCTGCATTGG AAATACATTG TGTCAGAAGC 
     2401 TACAAAATTT GAATATCTTC TTAGTAGGAT GTGGTGCCAT AGGCTGTGAA ATGTTAAAAA 
     2461 ATTTTGCATT ACTGGGTGTT GGTACAGGCA GAGAGAAAGG AATGGTAACA GTTACAGATC 
     2521 CTGACTTGAT AGAAAAATCC AATTTAAACA GACAGTTCCT GTTTCGTCCT CATCACATAC 
     2581 AGAAACCTAA AAGCTATACT GCTGCTGAAG CAACTCTGAA AATAAATCCT CAATTAAAAA 
     2641 TAGATGCACA CCTGAACAAA GTTTGTCCAG CCACTGAGAG TATATACAGT GATGAATTTT 
     2701 ACACTAAGCA AGATATCATT ATTACAGCAC TAGATAATGT GGAAGCCAGG AGATATGTAG 
     2761 ACAGTCGCTG TTTAGCAAAT CTACGGCCTC TCTTAGATTC TGGAACAATG GGCACCAAGG 
     2821 GACATACTGA AATTATCGTA CCTCAATTAA CTGAATCTTA TAATAGTCAT CGAGACCCCC 
     2881 CCGAAGAGGA AATACCATTT TGCACTCTAA AGTCCTTTCC AGCAGCTATC GAACATACTA 
     2941 TCCAGTGGGC AAGAGACAAG TTTGAAAGTT CCTTCTCCCA CAAACCTTCA CTGTTTAACA 
     3001 AGTTTTGGCA AGCATATCCC TCTGCAGAAG ATGTCTTACA GAAGATACAA AATGGACAAA 
     3061 GTTTAGAAGG CTGTTTTCAA GTTATTAAGC TACTTAGCAG AAGACCTAGA ATTTGGTCCC 
     3121 AGTGTGTAGA GTTAGCAAGA CTAAAGTTTG AAAAATACTT TAACCATAAG GCTCTGCAGC 
     3181 TTCTTCACTG TTTTCCTCTT GAGACAAGAC TAAAAGATGG CAGTTTGTTT TGGCAATCAC 
     3241 CAAAAAGGCC GCCTTCTCCA ATAAAATTTG ATTTAAATGA ACCTTTGCAC CTCAGTTTTC 
     3301 TTCAGAGTGC TGCAAAACTA TATGCTACAG TATATTGTAT TCCGTTTTCA GAAAAGGACT 
     3361 TATCAGTGAA TAGCCTCATG GACATTCTTT CAGAAGTTAA AATTGAGGAA TTCAAGCCTT 
     3421 CCAACAAGGT TGTTCAAACA GACGAAACTG CAAGGAAGCC CGACCATGTT CCTGTCTCCA 
     3481 GTGAAGATGA AAGGAATGCT GTTTTCCAAC TAGAAGAGGC TCTTTCCTCT AACAAAGCTA 
     3541 CCAAAAGTGA CCTTCAGATG ACAGTGCTTT CATTTGAAAA AGATGATGAC CGTAATGGAC 
     3601 ACATAGATTT CATCACAGCT GCTTCCAACC TTCGAGCCAA AATGTATAGC ATTGAACCAG 
     3661 CTGACCGTTT TAAAACTAAA CGCATAGCTG GTAAAATTAT ACCTGCTATA GCAACATCTA 
     3721 CTGCTGCAGT TTCTGGCTTG GTTGCTTTGG AGATGATCAA GGTAGCTGGT GGCTATCCCT 
     3781 TCGACGCTTA CAAAAACTGT TTCCTTAATT TAGCCATTCC AATAATAGTG TTTACAGAGA 
     3841 CATCTGAAGT AAGAAAAACT GAAATCAGAA ATGGAATATC ATTTACAATT TGGGACAGAT 
     3901 GGACTGTACA TGGAAAAGAA GATTTCACCC TCTCAGATTT CATAAATGCT GTCAAAGAAA 
     3961 ACTATGGAAT TGAGCCGACA ATGGTGGTGC AGGGAGTCAA AATGCTTTAT GTCCCTGTAA 
     4021 TGCCAGGTCA TGCAAAAAGG CTAAAGTTAA CAATGCACAA ACTTGTGAAG CCTTCCACTG 
     4081 AGAAGAAATA TGTGGATCTT ACTGTGTCAT TTGCCCCAGA CGCTGATGGA GATGAAGATT 
     4141 TGCCCGGCCC TCCAGTGAGA TACTACTTCA GTCATGACAC TAATGAGACG CGTACGCGGC 
     4201 CGCTCGAGCA GAAACTCATC TCAGAAGAGG ATCTGGCAGC AAATGATATC CTGGATTACA 
     4261 AGGATGACGA CGATAAGGTT TAAACGGCCG GCCGCGGTCA TAGCTGTTTC CTGAACAGAT 
     4321 CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA GTGCCTCTCC TGGCCCTGGA AGTTGCCACT 
     4381 CCAGTGCCCA CCAGCCTTGT CCTAATAAAA TTAAGTTGCA TCATTTTGTC TGACTAGGTG 
     4441 TCCTTCTATA ATATTATGGG GTGGAGGGGG GTGGTATGGA GCAAGGGGCA AGTTGGGAAG 
     4501 ACAACCTGTA GGGCCTGCGG GGTCTATTGG GAACCAAGCT GGAGTGCAGT GGCACAATCT 
     4561 TGGCTCACTG CAATCTCCGC CTCCTGGGTT CAAGCGATTC TCCTGCCTCA GCCTCCCGAG 
     4621 TTGTTGGGAT TCCAGGCATG CATGACCAGG CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA 
     4681 CGGGGTTTCA CCATATTGGC CAGGCTGGTC TCCAACTCCT AATCTCAGGT GATCTACCCA 
     4741 CCTTGGCCTC CCAAATTGCT GGGATTACAG GCGTGAACCA CTGCTCCCTT CCCTGTCCTT 
     4801 CTGATTTTAA AATAACTATA CCAGCAGGAG GACGTCCAGA CACAGCATAG GCTACCTGGC 
     4861 CATGCCCAAC CGGTGGGACA TTTGAGTTGC TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG 
     4921 TCCACTCAGT AGATGCCTGT TGAATTGGGT ACGCGGCCAG CGGCGAGCGG TATCAGCTCA 
     4981 CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG 
     5041 AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA 
     5101 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA 
     5161 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC 
     5221 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC 
     5281 GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT 
     5341 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG 
     5401 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG 
     5461 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA 
     5521 CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG 
     5581 AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT 
     5641 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT 
     5701 TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG 
     5761 ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT 
     5821 CTAAAGTATA TATGAGTAAC CTGAGGCTAT GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC 
     5881 GAGCCCTGGG CCTTCACCCG AACTTGGGGG GTGGGGTGGG GAAAAGGAAG AAACGCGGGC 
     5941 GTATTGGCCC CAATGGGGTC TCGGTGGGGT ATCGACAGAG TGCCAGCCCT GGGACCGAAC 
     6001 CCCGCGTTTA TGAACAAACG ACCCAACACC GTGCGTTTTA TTCTGTCTTT TTATTGCCGT 
     6061 CATAGCGCGG GTTCCTTCCG GTATTGTCTC CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG 
     6121 GTGGGCGAAG AACTCCAGCA TGAGATCCCC GCGCTGGAGG ATCATCCAGC CGGCGTCCCG 
     6181 GAAAACGATT CCGAAGCCCA ACCTTTCATA GAAGGCGGCG GTGGAATCGA AATCTCGTGA 
     6241 TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT TTCGAACCCC AGAGTCCCGC TCAGAAGAAC 
     6301 TCGTCAAGAA GGCGATAGAA GGCGATGCGC TGCGAATCGG GAGCGGCGAT ACCGTAAAGC 
     6361 ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA AGCTCTTCAG CAATATCACG GGTAGCCAAC 
     6421 GCTATGTCCT GATAGCGATC CGCCACACCC AGCCGGCCAC AGTCGATGAA TCCAGAAAAG 
     6481 CGGCCATTTT CCACCATGAT ATTCGGCAAG CAGGCATCGC CATGGGTCAC GACGAGATCC 
     6541 TCGCCGTCGG GCATGCTCGC CTTGAGCCTG GCGAACAGTT CGGCTGGCGC GAGCCCCTGA 
     6601 TGCTCTTCGT CCAGATCATC CTGATCGACA AGACCGGCTT CCATCCGAGT ACGTGCTCGC 
     6661 TCGATGCGAT GTTTCGCTTG GTGGTCGAAT GGGCAGGTAG CCGGATCAAG CGTATGCAGC 
     6721 CGCCGCATTG CATCAGCCAT GATGGATACT TTCTCGGCAG GAGCAAGGTG AGATGACAGG 
     6781 AGATCCTGCC CCGGCACTTC GCCCAATAGC AGCCAGTCCC TTCCCGCTTC AGTGACAACG 
     6841 TCGAGCACAG CTGCGCAAGG AACGCCCGTC GTGGCCAGCC ACGATAGCCG CGCTGCCTCG 
     6901 TCTTGCAGTT CATTCAGGGC ACCGGACAGG TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC 
     6961 TGCGCTGACA GCCGGAACAC GGCGGCATCA GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA 
     7021 TAGCCGAATA GCCTCTCCAC CCAAGCGGCC GGAGAACCTG CGTGCAATCC ATCTTGTTCA 
     7081 ATCATGCGAA ACGATCCTCA TCCTGTCTCT TGATCGATCT TTGCAAAAGC CTAGGCCTCC 
     7141 AAAAAAGCCT CCTCACTACT TCTGGAATAG CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC 
     7201 ATAAATAAAA AAAATTAGTC AGCCATGGGG CGGAGAATGG GCGGAACTGG GCGGAGTTAG 
     7261 GGGCGGGATG GGCGGAGTTA GGGGCGGGAC TATGGTTGCT GACTAATTGA GATGCATGCT 
     7321 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCTGGTTG CTGACTAATT 
     7381 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCCTAA 
     7441 CTGACACACA TTCCACAGCT GGTTCTTTCC GCCTCAGGAC TCTTCCTTTT TCAATATTAT 
     7501 TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA 
     7561 AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA CGCGCCCTGT 
     7621 AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC 
     7681 AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC 
     7741 TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG 
     7801 CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA 
     7861 TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC 
     7921 CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT TTGATTTATA AGGGATTTTG 
     7981 CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//