LOCUS       MR211529                8025 bp    DNA     circular  SYN 
DEFINITION  Gucy2c (Myc-DDK-tagged) - Mouse guanylate cyclase 2c (Gucy2c), transcript variant 2
ACCESSION   NM_145067
VERSION     NM_145067.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4182..4273 
                     /label="Myc-DDK"  
     misc_feature    4303..4888 
                     /label="PolyA signal"  
     misc_feature    4360..4379 
                     /label="Restriction Enzyme"  
     misc_feature    5164..5836 
                     /label="ColE1"  
     misc_feature    6266..7060 
                     /label="Kan/Neo"  
     misc_feature    7095..7397 
                     /label="SV40 ori"  
     misc_feature    7483..7960 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4164..4170 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4164 
                     /label="Gucy2c"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GACGTCACTG CTGGGCTTGG CTGTGCGGTT ACTGCTCTTC CAGCCCGCGC 
     1081 TGATGGTGTT CTGGGCCTCT CAGGTGAGGC AGAACTGCCG CAATGGCAGC TACGAGATCA 
     1141 GCGTCCTGAT GATGGACAAC TCAGCCTACA AAGAACCTAT GCAAAACCTG AGGGAGGCTG 
     1201 TGGAGGAAGG ACTGGACATA GTGCGAAAGC GCCTGCGTGA AGCCGACCTA AATGTGACTG 
     1261 TGAACGCGAC TTTCATCTAC TCCGACGGTC TGATTCATAA GTCAGGTGAC TGCCGGAGCA 
     1321 GCACCTGTGA AGGCCTTGAC CTACTCAGGG AGATTACAAG AGATCATAAG ATGGGCTGCG 
     1381 CCCTCATGGG GCCCTCGTGC ACGTATTCCA CCTTCCAGAT GTACCTCGAC ACAGAGTTGA 
     1441 ACTATCCCAT GATTTCCGCT GGAAGTTATG GATTGTCCTG TGACTATAAG GAAACCCTAA 
     1501 CCAGGATCCT GCCTCCAGCC AGGAAGCTGA TGTACTTCTT GGTCGATTTC TGGAAAGTCA 
     1561 ACAATGCATC TTTCAAACCC TTTTCCTGGA ACTCTTCGTA TGTTTACAAG AATGGATCGG 
     1621 AACCTGAAGA TTGTTTCTGG TACCTCAATG CTCTGGAGGC TGGGGTGTCC TATTTTTCTG 
     1681 AGGTGCTCAA CTTCAAGGAT GTACTGAGAC GCAGCGAACA GTTCCAGGAA ATCTTAACAG 
     1741 GCCATAACAG AAAGAGCAAT GTGATTGTTA TGTGTGGCAC GCCAGAAAGC TTCTATGATG 
     1801 TGAAAGGTGA CCTCCAAGTG GCTGAAGATA CTGTTGTCAT CCTGGTAGAT CTGTTCAGTA 
     1861 ACCATTACTT TGAGGAGAAC ACCACAGCTC CTGAGTATAT GGACAATGTC CTCGTCCTGA 
     1921 CGCTGCCGTC TGAACAGTCC ACCTCAAACA CCTCTGTCGC CGAGAGGTTT TCATCGGGGA 
     1981 GAAGTGACTT TTCTCTCGCT TACTTGGAGG GAACCTTGCT ATTTGGACAC ATGCTGCAGA 
     2041 CGTTTCTTGA AAATGGAGAA AATGTCACGG GTCCCAAGTT TGCTCGTGCA TTCAGGAATC 
     2101 TCACTTTTCA AGGCTTTGCA GGACCTGTGA CTCTGGATGA CAGTGGGGAC ATTGACAACA 
     2161 TTATGTCCCT TCTGTATGTG TCTCTGGATA CCAGGAAATA CAAGGTTCTT ATGAAGTATG 
     2221 ACACCCACAA AAACAAAACT ATTCCGGTGG CTGAGAACCC CAACTTCATC TGGAAGAACC 
     2281 ACAAGCTCCC CAATGACGTT CCTGGGCTGG GCCCTCAAAT CCTGATGATT GCCGTCTTCA 
     2341 CGCTCACGGG GATCCTGGTA GTTCTGCTGC TGATTGCCCT CCTCGTGCTG AGAAAATACA 
     2401 GAAGAGATCA TGCACTTCGA CAGAAGAAAT GGTCCCACAT TCCTTCTGAA AACATCTTTC 
     2461 CTCTGGAGAC CAACGAGACC AACCACATCA GCCTGAAGAT TGACGATGAC AGGAGACGAG 
     2521 ACACAATCCA GAGAGTGCGA CAGTGCAAAT ACGACAAGAA GTTGCTGCAG TCTGACTACT 
     2581 ACAACCTGAC TAAGTTCTAC GGCACCGTGA AGCTGGACAC CAGGATCTTT GGGGTGGTTG 
     2641 AGTACTGCGA GAGGGGATCC CTCCGGGAAG TGTTAAACGA CACAATTTCC TACCCTGACG 
     2701 GCACGTTCAT GGATTGGGAG TTTAAGATCT CTGTCTTAAA TGACATCGCT AAGGGGATGT 
     2761 CCTACCTGCA CTCCAGTAAG ATTGAAGTCC ACGGGCGTCT CAAATCCACC AACTGCGTGG 
     2821 TGGACAGCCG CATGGTGGTG AAGATCACCG ACTTTGGGTG CAATTCCATC CTGCCTCCAA 
     2881 AAAAAGACCT GTGGACGGCC CCGGAGCACC TGCGCCAGGC CACCATCTCT CAGAAAGGAG 
     2941 ACGTGTACAG CTTCGCCATC ATTGCCCAGG AGATCATCCT CCGTAAGGAG ACTTTTTACA 
     3001 CGCTGAGCTG TCGGGATCAC AATGAGAAGA TTTTCAGAGT GGAAAATTCA TACGGGAAAC 
     3061 CTTTCCGCCC AGACCTCTTC CTGGAGACTG CAGATGAGAA GGAGCTGGAG GTCTATCTAC 
     3121 TTGTCAAAAG CTGTTGGGAG GAGGATCCAG AAAAGAGGCC AGATTTCAAG AAAATCGAGA 
     3181 GCACACTGGC CAAGATATTT GGCCTTTTCC ATGACCAGAA AAACGAGTCT TACATGGACA 
     3241 CCTTGATCCG ACGTCTCCAG CTGTACTCTC GAAACCTGGA ACATCTGGTG GAGGAAAGGA 
     3301 CTCAGCTGTA CAAGGCGGAG AGGGACAGGG CTGACCACCT TAACTTCATG CTCCTCCCAC 
     3361 GGCTGGTGGT AAAGTCACTG AAGGAGAAAG GCATCGTGGA GCCAGAGCTG TACGAAGAAG 
     3421 TCACAATCTA CTTCAGTGAC ATTGTGGGCT TCACCACCAT CTGCAAGTAT AGCACGCCCA 
     3481 TGGAGGTGGT GGACATGCTC AACGACATCT ACAAGAGCTT TGACCAGATT GTGGACCACC 
     3541 ATGACGTCTA CAAGGTAGAA ACCATCGGTG ACGCCTACGT GGTGGCCAGC GGTCTGCCTA 
     3601 TGAGAAACGG CAACCGACAC GCGGTAGACA TTTCCAAGAT GGCCTTGGAC ATCCTCAGCT 
     3661 TCATAGGGAC CTTTGAGTTG GAGCATCTCC CTGGCCTCCC CGTGTGGATC CGCATTGGAG 
     3721 TTCATTCTGG GCCCTGCGCT GCTGGTGTTG TGGGGATCAA GATGCCTCGC TATTGCCTGT 
     3781 TTGGAGACAC TGTCAACACT GCCTCCAGGA TGGAATCCAC CGGCCTCCCC TTGAGGATTC 
     3841 ACATGAGCAG CTCCACCATA ACCATCCTGA AGAGAACGGA TTGCCAGTTC CTGTATGAAG 
     3901 TGAGGGGAGA AACCTACTTA AAGGGAAGAG GGACAGAGAC CACATACTGG CTGACTGGGA 
     3961 TGAAGGACCA AGAATACAAC CTGCCATCCC CACCGACAGT GGAGAACCAA CAGCGTCTGC 
     4021 AGACTGAGTT CTCAGACATG ATCGTTAGCG CCTTACAGAA AAGACAGGCC TCGGGCAAGA 
     4081 AGAGCCGGAG GCCCACTCGG GTGGCCAGCT ACAAGAAAGG CTTTCTGGAA TACATGCAGC 
     4141 TGAACAATTC AGACCACGAT AGCACCTATT TTACGCGTAC GCGGCCGCTC GAGCAGAAAC 
     4201 TCATCTCAGA AGAGGATCTG GCAGCAAATG ATATCCTGGA TTACAAGGAT GACGACGATA 
     4261 AGGTTTAAAC GGCCGGCCGC GGTCATAGCT GTTTCCTGAA CAGATCCCGG GTGGCATCCC 
     4321 TGTGACCCCT CCCCAGTGCC TCTCCTGGCC CTGGAAGTTG CCACTCCAGT GCCCACCAGC 
     4381 CTTGTCCTAA TAAAATTAAG TTGCATCATT TTGTCTGACT AGGTGTCCTT CTATAATATT 
     4441 ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG GGGCAAGTTG GGAAGACAAC CTGTAGGGCC 
     4501 TGCGGGGTCT ATTGGGAACC AAGCTGGAGT GCAGTGGCAC AATCTTGGCT CACTGCAATC 
     4561 TCCGCCTCCT GGGTTCAAGC GATTCTCCTG CCTCAGCCTC CCGAGTTGTT GGGATTCCAG 
     4621 GCATGCATGA CCAGGCTCAG CTAATTTTTG TTTTTTTGGT AGAGACGGGG TTTCACCATA 
     4681 TTGGCCAGGC TGGTCTCCAA CTCCTAATCT CAGGTGATCT ACCCACCTTG GCCTCCCAAA 
     4741 TTGCTGGGAT TACAGGCGTG AACCACTGCT CCCTTCCCTG TCCTTCTGAT TTTAAAATAA 
     4801 CTATACCAGC AGGAGGACGT CCAGACACAG CATAGGCTAC CTGGCCATGC CCAACCGGTG 
     4861 GGACATTTGA GTTGCTTGCT TGGCACTGTC CTCTCATGCG TTGGGTCCAC TCAGTAGATG 
     4921 CCTGTTGAAT TGGGTACGCG GCCAGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT 
     4981 ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA 
     5041 AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC 
     5101 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA 
     5161 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC 
     5221 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC 
     5281 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA 
     5341 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC 
     5401 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG 
     5461 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG 
     5521 AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG 
     5581 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA 
     5641 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA 
     5701 CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT 
     5761 CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA 
     5821 GTAACCTGAG GCTATGGCAG GGCCTGCCGC CCCGACGTTG GCTGCGAGCC CTGGGCCTTC 
     5881 ACCCGAACTT GGGGGGTGGG GTGGGGAAAA GGAAGAAACG CGGGCGTATT GGCCCCAATG 
     5941 GGGTCTCGGT GGGGTATCGA CAGAGTGCCA GCCCTGGGAC CGAACCCCGC GTTTATGAAC 
     6001 AAACGACCCA ACACCGTGCG TTTTATTCTG TCTTTTTATT GCCGTCATAG CGCGGGTTCC 
     6061 TTCCGGTATT GTCTCCTTCC GTGTTTCAGT TAGCCTCCCC CTAGGGTGGG CGAAGAACTC 
     6121 CAGCATGAGA TCCCCGCGCT GGAGGATCAT CCAGCCGGCG TCCCGGAAAA CGATTCCGAA 
     6181 GCCCAACCTT TCATAGAAGG CGGCGGTGGA ATCGAAATCT CGTGATGGCA GGTTGGGCGT 
     6241 CGCTTGGTCG GTCATTTCGA ACCCCAGAGT CCCGCTCAGA AGAACTCGTC AAGAAGGCGA 
     6301 TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG GCGATACCGT AAAGCACGAG GAAGCGGTCA 
     6361 GCCCATTCGC CGCCAAGCTC TTCAGCAATA TCACGGGTAG CCAACGCTAT GTCCTGATAG 
     6421 CGATCCGCCA CACCCAGCCG GCCACAGTCG ATGAATCCAG AAAAGCGGCC ATTTTCCACC 
     6481 ATGATATTCG GCAAGCAGGC ATCGCCATGG GTCACGACGA GATCCTCGCC GTCGGGCATG 
     6541 CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT GGCGCGAGCC CCTGATGCTC TTCGTCCAGA 
     6601 TCATCCTGAT CGACAAGACC GGCTTCCATC CGAGTACGTG CTCGCTCGAT GCGATGTTTC 
     6661 GCTTGGTGGT CGAATGGGCA GGTAGCCGGA TCAAGCGTAT GCAGCCGCCG CATTGCATCA 
     6721 GCCATGATGG ATACTTTCTC GGCAGGAGCA AGGTGAGATG ACAGGAGATC CTGCCCCGGC 
     6781 ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC GCTTCAGTGA CAACGTCGAG CACAGCTGCG 
     6841 CAAGGAACGC CCGTCGTGGC CAGCCACGAT AGCCGCGCTG CCTCGTCTTG CAGTTCATTC 
     6901 AGGGCACCGG ACAGGTCGGT CTTGACAAAA AGAACCGGGC GCCCCTGCGC TGACAGCCGG 
     6961 AACACGGCGG CATCAGAGCA GCCGATTGTC TGTTGTGCCC AGTCATAGCC GAATAGCCTC 
     7021 TCCACCCAAG CGGCCGGAGA ACCTGCGTGC AATCCATCTT GTTCAATCAT GCGAAACGAT 
     7081 CCTCATCCTG TCTCTTGATC GATCTTTGCA AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA 
     7141 CTACTTCTGG AATAGCTCAG AGGCCGAGGC GGCCTCGGCC TCTGCATAAA TAAAAAAAAT 
     7201 TAGTCAGCCA TGGGGCGGAG AATGGGCGGA ACTGGGCGGA GTTAGGGGCG GGATGGGCGG 
     7261 AGTTAGGGGC GGGACTATGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     7321 TGCTGGGGAG CCTGGGGACT TTCCACACCT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     7381 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CCTAACTGAC ACACATTCCA 
     7441 CAGCTGGTTC TTTCCGCCTC AGGACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG 
     7501 GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG 
     7561 GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGCGC CCTGTAGCGG CGCATTAAGC 
     7621 GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC 
     7681 GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT 
     7741 CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA 
     7801 AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC 
     7861 CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA 
     7921 CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT 
     7981 TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTT
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