LOCUS       MR211506                7998 bp    DNA     circular  SYN 
DEFINITION  Man2c1 (Myc-DDK-tagged) - Mouse mannosidase, alpha, class 2C, member 1 (Man2c1)
ACCESSION   NM_028636
VERSION     NM_028636.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4155..4246 
                     /label="Myc-DDK"  
     misc_feature    4276..4861 
                     /label="PolyA signal"  
     misc_feature    4333..4352 
                     /label="Restriction Enzyme"  
     misc_feature    5137..5809 
                     /label="ColE1"  
     misc_feature    6239..7033 
                     /label="Kan/Neo"  
     misc_feature    7068..7370 
                     /label="SV40 ori"  
     misc_feature    7456..7933 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4137..4143 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4137 
                     /label="Man2c1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGGCAGCG CCGTTCTTGA AGCACTGGCG CACCACTTTC GAGCGGGTGG 
     1081 AGAAGTTCGT GTCCCCGATC TACTTCACCG ACTGTAACCT TCGCGGCAGG CTCTTCGGGG 
     1141 ACAGCTGCTC GGTGACACTC TCCAGCTTCC TGACGCCCGA GAGGCTTCCC TATGAGAAGG 
     1201 CGGTGCAGCA GAACTTCAGC CCTGCGCAGG TCGGCGACAG CTTCGGACCC ACATGGTGGA 
     1261 CCTGCTGGTT TCGGGTAGAA CTGGTCATCC CTGAAGTTTG GGTGGGCCAG GAAGTTCATC 
     1321 TTTGCTGGGA AAGCGATGGA GAAAGCCTTG TCTGGCGTGA TGGGGAACCT GTCCAGGGTT 
     1381 TGACCAAAGA AGGCGAAAAG ACCAGTTATG TCCTGAGCGA GAGGCTGCGA GCATCAGACC 
     1441 CTCGGAGCTT GACTCTCTAT GTGGAAGTAG CCTGCAATGG GCTTCTGGGG GCCGGGAAGG 
     1501 GAAGCATGAT CGCAGCCCCT GACCCAGAGA AGATGTTCCA GCTGAGCCAG GCTAAACTGG 
     1561 CTGTGTTCCA CCGGGATGTC CACAGTCTCC TGGTGGACCT GGAGCTGTTG CTGGGTGTGG 
     1621 CCAAGGGCCT CGGGGAGGAC AGCCAGCGCA GCTTCCAGGC CCTGCACACA GCCAACCAGA 
     1681 TGGTGAACAT ATGTGACCCT GCTCAGCCTG AGACTTACCC AGCAGCCAAA GCCCTGGCCT 
     1741 CCAAGTTCTT TGGCCAACAT GGAGGTGAAA GCCAGCATAC CATCCATGCC ATGGGACACT 
     1801 GTCACATTGA TACAGCCTGG CTTTGGCCCT TCAAGGAGAC TGTGAGGAAA TGTGCCCGGA 
     1861 GCTGGTCGAC AGCTGTGACG CTCATGGAGC AGAACACAGA CTTCATCTTT GCCTGCTCTC 
     1921 AGGCACAACA ACTTGAATGG GTCAAGAGCC AGTACCCTGG TCTGCATGCC CGGCTCCAGG 
     1981 AGTTTGCCTG CCGAGGACAG TTTGTGCCTG TGGGGGGCAC CTGGGTAGAA ATGGATGGGA 
     2041 ACCTTCCCAG TGGAGAGGCC ATGGTGAGGC AGTTCCTGCA GGGCCAGAAC TTCTTTCTGC 
     2101 AAGAGTTTGG GAAGATGTGC TCTGAGTTCT GGCTGCCAGA CACGTTTGGC TACTCAGCAC 
     2161 AGCTCCCCCA GATCATGCAA GGCTGTGGCA TCAAGCGCTT CCTGACCCAG AAACTGAGCT 
     2221 GGAACTTGGT CAACTCCTTT CCACACCATA CCTTTTTCTG GGAGGGGCTG GATGGCTCCC 
     2281 GAGTGCTGGT CCACTTCCCA CCTGGAGACT CATATGGAAT GCAGGGCAGT GTGGAGGAGG 
     2341 TGCTGAAGAC TGTGACCAAC AACCGGGACA AGGGGCGGAC CAACCACAGT GGCTTCCTCT 
     2401 TCGGCTTCGG GGATGGGGGA GGTGGCCCCA CACAGACCAT GTTGGACCGA TTGAAGCGGC 
     2461 TGAGCAACAC TGATGGGCTG CCCAGGGTGC AGCTATCTTC TCCTGGCCAG CTCTTCACGG 
     2521 CACTGGAGAG AGACTCAGGA CAGCTGTGCA CATGGGTTGG AGAACTCTTC CTGGAACTGC 
     2581 ACAATGGCAC CTACACTACG CATGCCCAGC TCAAGAAGGG GAACAGAGAA TGTGAGCAGA 
     2641 TTCTTCATGA CGTGGAGGTG CTCAGCAGCC TGGCCCTAGC CCGAAGTGCC CAGTTCCTAT 
     2701 ACCCGGCAGC CCAGCTGCAG CACCTCTGGA GGCTCCTGCT CCTCAACCAG TTCCATGATG 
     2761 TGGTGACTGG AAGCTGCATT CAGCTGGTGG CAGAAGATGC CATGAACTAC TATGAAGACA 
     2821 TCCGTTCTCA TGGCAACCCA CTGCTCAGTG CTGCAGCCGC AGCCTTGTGT GCCGGGGAGC 
     2881 CAGGTCCCAA GGGCCTCCTC ATTATCAACA CACTGCCCTG GAAGCGCACT GAAGTGTTGG 
     2941 CACTGCCCAA GCCTTGTGGG GCTCACAGCC TAGCCCTGGT GACAGTGCCA AGTATTGGCT 
     3001 ACGCTCCTGC TCCCACCCCT ACTTCACTGC AGCCCCTCCT CCCCCAGCAG CCTGTGTTTG 
     3061 TGATGCAGGA GACTGACGGT TCTGTGACTT TGGACAACGG CATCATCCGG GTGAGACTGG 
     3121 ATCCAACTGG CTGTCTTACC TCCCTGGTCC TGGTGGCTTC TGGCAGGGAA GCCATTGCTG 
     3181 AGGGAGCCCT GGGGAACCAG TTTGTGTTGT TTGATGATGT GCCATTGTAC TGGGATGCAT 
     3241 GGGATGTCAT GGATTACCAT CTAGAGACAC GGAAGCCTGT GCTGGGCCAG GCAGGGACCC 
     3301 TGGCAGTGGG CACTGAAGGT GGCCTCCGGG GCAGTGCCTG GTTCCTGCTC CAGATCAGCC 
     3361 CCAACAGCCG GCTCAGCCAG GAGGTTGTGC TAGATGTTGG CTGCCCCTAT GTCCGCTTCC 
     3421 ACACTGAGGT GCACTGGCAC GAGGCCCACA AGTTCCTGAA GGTGGAGTTC CCTGCTAGAA 
     3481 TACGGAGCCC CCAGGCTACC TATGAGATCC AGTTTGGGCA CCTCCAGAGG CCGACGCACA 
     3541 ACAACACCTC TTGGGACTGG GCTCGCTACG AGGTGTGGGC TCACCGCTGG ATAGATCTGT 
     3601 CCGAGTGTGA CTTTGGGCTG GCCCTGCTCA ACAACTGCAA GTATGGAGCA TCAGTACGAG 
     3661 GCAACGTCCT CAGCCTCTCA CTGTTGCGTG CACCCAAGGC TCCAGACGCT ACTGCTGACA 
     3721 TGGGGCGCCA TGAATTCACT TACGCTTTGA TGCCACACAA GGGCTCCTTC CAAGAAGCTG 
     3781 GTGTCATCCA TGCTGCCTAC AACCTCAACT TTCCCCTGCT GGCACTGCCG GCTCCAGGCC 
     3841 CGGCCCCTGA CACCACCTGG AGTGCCTTTT CTGTGTCTTC ACCAGCAGTA GTGCTGGAGA 
     3901 CCATCAAGCA GGCTGAGAGG TGCCATCAGC ACCGCACACT GGTTCTAAGG CTGTACGAGG 
     3961 CTCATGGCAG CCATGTGGAC TGCTGGCTGC ACACGTCGTT GCCTGTTCAG GAGGCCACCC 
     4021 TTTGTGACCT CCTGGAGCAG CGAGACCCCA CTGGTCACCT TTCCCTCCAG GACAACCGTC 
     4081 TGAAGCTCAC TTTTTCTCCC TTCCAAGTGA GATCCCTATT GCTTGTGCTT CAGTCTCCAC 
     4141 CAAACACGCG TACGCGGCCG CTCGAGCAGA AACTCATCTC AGAAGAGGAT CTGGCAGCAA 
     4201 ATGATATCCT GGATTACAAG GATGACGACG ATAAGGTTTA AACGGCCGGC CGCGGTCATA 
     4261 GCTGTTTCCT GAACAGATCC CGGGTGGCAT CCCTGTGACC CCTCCCCAGT GCCTCTCCTG 
     4321 GCCCTGGAAG TTGCCACTCC AGTGCCCACC AGCCTTGTCC TAATAAAATT AAGTTGCATC 
     4381 ATTTTGTCTG ACTAGGTGTC CTTCTATAAT ATTATGGGGT GGAGGGGGGT GGTATGGAGC 
     4441 AAGGGGCAAG TTGGGAAGAC AACCTGTAGG GCCTGCGGGG TCTATTGGGA ACCAAGCTGG 
     4501 AGTGCAGTGG CACAATCTTG GCTCACTGCA ATCTCCGCCT CCTGGGTTCA AGCGATTCTC 
     4561 CTGCCTCAGC CTCCCGAGTT GTTGGGATTC CAGGCATGCA TGACCAGGCT CAGCTAATTT 
     4621 TTGTTTTTTT GGTAGAGACG GGGTTTCACC ATATTGGCCA GGCTGGTCTC CAACTCCTAA 
     4681 TCTCAGGTGA TCTACCCACC TTGGCCTCCC AAATTGCTGG GATTACAGGC GTGAACCACT 
     4741 GCTCCCTTCC CTGTCCTTCT GATTTTAAAA TAACTATACC AGCAGGAGGA CGTCCAGACA 
     4801 CAGCATAGGC TACCTGGCCA TGCCCAACCG GTGGGACATT TGAGTTGCTT GCTTGGCACT 
     4861 GTCCTCTCAT GCGTTGGGTC CACTCAGTAG ATGCCTGTTG AATTGGGTAC GCGGCCAGCG 
     4921 GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA 
     4981 CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC 
     5041 GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC 
     5101 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG 
     5161 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT 
     5221 CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA 
     5281 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC 
     5341 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC 
     5401 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT 
     5461 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT 
     5521 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC 
     5581 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA 
     5641 AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 
     5701 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 
     5761 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAACCT GAGGCTATGG CAGGGCCTGC 
     5821 CGCCCCGACG TTGGCTGCGA GCCCTGGGCC TTCACCCGAA CTTGGGGGGT GGGGTGGGGA 
     5881 AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA ATGGGGTCTC GGTGGGGTAT CGACAGAGTG 
     5941 CCAGCCCTGG GACCGAACCC CGCGTTTATG AACAAACGAC CCAACACCGT GCGTTTTATT 
     6001 CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC 
     6061 AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA CTCCAGCATG AGATCCCCGC GCTGGAGGAT 
     6121 CATCCAGCCG GCGTCCCGGA AAACGATTCC GAAGCCCAAC CTTTCATAGA AGGCGGCGGT 
     6181 GGAATCGAAA TCTCGTGATG GCAGGTTGGG CGTCGCTTGG TCGGTCATTT CGAACCCCAG 
     6241 AGTCCCGCTC AGAAGAACTC GTCAAGAAGG CGATAGAAGG CGATGCGCTG CGAATCGGGA 
     6301 GCGGCGATAC CGTAAAGCAC GAGGAAGCGG TCAGCCCATT CGCCGCCAAG CTCTTCAGCA 
     6361 ATATCACGGG TAGCCAACGC TATGTCCTGA TAGCGATCCG CCACACCCAG CCGGCCACAG 
     6421 TCGATGAATC CAGAAAAGCG GCCATTTTCC ACCATGATAT TCGGCAAGCA GGCATCGCCA 
     6481 TGGGTCACGA CGAGATCCTC GCCGTCGGGC ATGCTCGCCT TGAGCCTGGC GAACAGTTCG 
     6541 GCTGGCGCGA GCCCCTGATG CTCTTCGTCC AGATCATCCT GATCGACAAG ACCGGCTTCC 
     6601 ATCCGAGTAC GTGCTCGCTC GATGCGATGT TTCGCTTGGT GGTCGAATGG GCAGGTAGCC 
     6661 GGATCAAGCG TATGCAGCCG CCGCATTGCA TCAGCCATGA TGGATACTTT CTCGGCAGGA 
     6721 GCAAGGTGAG ATGACAGGAG ATCCTGCCCC GGCACTTCGC CCAATAGCAG CCAGTCCCTT 
     6781 CCCGCTTCAG TGACAACGTC GAGCACAGCT GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC 
     6841 GATAGCCGCG CTGCCTCGTC TTGCAGTTCA TTCAGGGCAC CGGACAGGTC GGTCTTGACA 
     6901 AAAAGAACCG GGCGCCCCTG CGCTGACAGC CGGAACACGG CGGCATCAGA GCAGCCGATT 
     6961 GTCTGTTGTG CCCAGTCATA GCCGAATAGC CTCTCCACCC AAGCGGCCGG AGAACCTGCG 
     7021 TGCAATCCAT CTTGTTCAAT CATGCGAAAC GATCCTCATC CTGTCTCTTG ATCGATCTTT 
     7081 GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC TCACTACTTC TGGAATAGCT CAGAGGCCGA 
     7141 GGCGGCCTCG GCCTCTGCAT AAATAAAAAA AATTAGTCAG CCATGGGGCG GAGAATGGGC 
     7201 GGAACTGGGC GGAGTTAGGG GCGGGATGGG CGGAGTTAGG GGCGGGACTA TGGTTGCTGA 
     7261 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     7321 CCTGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     7381 GGACTTTCCA CACCCTAACT GACACACATT CCACAGCTGG TTCTTTCCGC CTCAGGACTC 
     7441 TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA 
     7501 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG 
     7561 CCACCTGACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC 
     7621 GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT 
     7681 CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC 
     7741 CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT 
     7801 AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT 
     7861 AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT 
     7921 GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA 
     7981 AAATTTAACG CGAATTTT
//