LOCUS       MR211491                7980 bp    DNA     circular  SYN 
DEFINITION  Kdm4a (Myc-DDK-tagged) - Mouse lysine (K)-specific demethylase 4A (Kdm4a), transcript variant 2
ACCESSION   NM_172382
VERSION     NM_172382.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4137..4228 
                     /label="Myc-DDK"  
     misc_feature    4258..4843 
                     /label="PolyA signal"  
     misc_feature    4315..4334 
                     /label="Restriction Enzyme"  
     misc_feature    5119..5791 
                     /label="ColE1"  
     misc_feature    6221..7015 
                     /label="Kan/Neo"  
     misc_feature    7050..7352 
                     /label="SV40 ori"  
     misc_feature    7438..7915 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4119..4125 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4119 
                     /label="Kdm4a"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCTTCTGAA TCAGAAACTT TGAACCCCAG TGCTCGGATC ATGACCTTTT 
     1081 ACCCCACCAT GGAAGAGTTC CGGAACTTCA GCCGCTACAT TGCCTACATT GAATCCCAAG 
     1141 GAGCTCATCG GGCTGGGCTA GCCAAGGTCG TTCCTCCCAA AGAGTGGAAG CCTCGAACAT 
     1201 CCTACGACGA TATTGATGAT CTAGTCATCC CTGCGCCCAT CCAGCAGCTG GTGACTGGCC 
     1261 AGTCTGGCCT CTTCACTCAG TACAACATAC AGAAGAAAGC CATGACCGTT CGTGAGTTCC 
     1321 GCAAGATCGC CAATAGCGAC AAGTACTGCA CCCCGCGATA CAGTGAATTT GAAGAACTTG 
     1381 AGCGTAAATA CTGGAAAAAC CTCACATTCA ATCCTCCCAT CTATGGAGCA GACGTGAATG 
     1441 GTACTCTCTA TGAACAGCAC GTTGATGAGT GGAATATCGG CCGGCTGAAG ACCATCCTGG 
     1501 ACCTGGTGGA GAAGGAGAGC GGGATCACCA TTGAGGGGGT GAACACCCCC TACCTTTACT 
     1561 TCGGCATGTG GAAGACGTCC TTCGCCTGGC ACACGGAAGA CATGGATCTA TACAGCATCA 
     1621 ACTATCTGCA CTTCGGAGAG CCAAAGTCTT GGTACTCTGT TCCACCTGAG CATGGGAAAC 
     1681 GCCTGGAGCG TCTTGCCAAA GGCTTTTTCC CAGGAAGTGC TCAAAGCTGT GAGGCTTTTC 
     1741 TCCGCCACAA GATGACCCTG ATCTCCCCAT TAATGCTGAA GAAGTATGGC ATTCCCTTTG 
     1801 ACAAGGTGAC CCAAGAAGCT GGCGAGTTTA TGATCACTTT TCCGTATGGT TACCATGCTG 
     1861 GCTTCAACCA TGGCTTCAAC TGTGCAGAGT CAACCAATTT TGCTACCCGT CGGTGGATTG 
     1921 AGTATGGCAA GCAGGCTGTG CTGTGCTCCT GCAGAAAAGA TATGGTGAAG ATCTCCATGG 
     1981 ACGTGTTCGT GAGGAGGTTC CAGCCGGAAC GCTACAAACT TTGGAAAGCT GGGAAGGACA 
     2041 GCATGGTTAT TGACCACACT CTGCCCACAC CGGAAGCAGC CGAGTTCCTG AAGGACAGCG 
     2101 GCGGACTAAC CCCGAGAGCA GGGAGTGAGG AGTGCCCAGA GGAGGACGTG GAAGCAGCGG 
     2161 ATCAGGGAGA GGAGGGGGAT GTGAAGAGAA GCCTGGCCAA GCATCGGATC GGGACAAAGA 
     2221 GACACAGAGT CTGTCTGGAA ATACCCCAGG AGGTCAGTCA GAGCGAGCTC TTCCCCAAGG 
     2281 AAGAGCTAAG TTCTGGACAG TATGAGATGA CAGAGTGCCC GGCCACCCTC GCCCCAGTGA 
     2341 GGCCCACCCA CAGCTCCGTG CGGCAAGTTG AGGACAGTCT TCCCTTCCCA GATTACTCTG 
     2401 ACCCCACTGA AGTCAAATTT GAAGAGCTGA AGAATGTCAA ACTAGAAGAG GAAGATGAGG 
     2461 AGGATGAGCC CGAAGCAGCC GCACTGGACC TTTCTGTGAA TCCTGCGTCT GTAGGAGGAC 
     2521 GCCTCGTCTT CTCGGGTTCC AAAAAGAAAT CATCTTCCAG CCTGGGCTCC ACTTCATCTC 
     2581 AGGATTCAGT TTCTTCAGAT TCTGAAACCG CCGAGTCTGT CTCTTGCCAG GGCCAAGAGA 
     2641 AAACGGGAGT TCTCACCGTG CACAGCTATG CCAGAGGGGA CGGCAAGGCT GCCACAGGAG 
     2701 AGCCGAGTGT GAAGAAGAAG CGCAGTGCTC CCCGCAGCAT CAGCGAGCAG GAGCTGGCAG 
     2761 AGGTTGCAGA TGAGTACATG CTTTCCCTGG AGGAGAATAA GAAGACCAAG GGTCGCCGCC 
     2821 AGCCCTTGAG CAAGCTCCCA CGCCACCACC CACTCGTGCT GCAGGAGTGC GGCAGCGATG 
     2881 ACGAGACTTC TGAGCAGCTG ACCCCCGAGG AGGAGGCTGA GGAGACAGAG GCTTGGGCCA 
     2941 AGCCTCTGAG CCAGCTGTGG CAGAACCGAC CCCCGAACTT TGAGGCTGAA AAGGAATTCA 
     3001 ATGAGATCAT GGCCCAACAG GCCCCTCACT GTGCTGTGTG TATGATCTTC CAGACCTACC 
     3061 ATCAGGTGGA ATTTGGAGCC TTCAGTCAGA GCTGTGGGGA TGCTTCAGAG CCGGCTGCCC 
     3121 AGACTCAGAG GACCAAGCCA CTGATCCCCG AAATGTGCTT TACCACCACT GGCTGCAGCA 
     3181 CAGACATCAA TCTGTCCACC CCGTACCTAG AGGAGGATGG CACCAGCATG CTAGTGTCCT 
     3241 GCAAGAAGTG CAGTGTCCGG GTCCACGCCA GTTGCTATGG CGTCCCTCCT GCCAAGGCTT 
     3301 CTGAAGAATG GATGTGCTCT CGATGTTCAG CCAATGCCCT GGAGGAAGAC TGCTGCTTAT 
     3361 GCTCGTTGCG TGGAGGGGCC CTGCAGAGAG CCAATGACGA CAGATGGGTT CATGTTTCAT 
     3421 GTGCAGTGGC AATTCTAGAA GCAAGGTTTG TCAACATTGC TGAAAGAAGT CCAGTGGATG 
     3481 TGAGCAAAAT CCCTCTGCCC CGCTTCAAAC TGAAGTGTGT TTTTTGTAAG AAGCGGAGAA 
     3541 AACGGAATGC TGGCTGCTGT GTGCAGTGCT CCCATGGCCG CTGCCCCACT GCCTTCCATG 
     3601 TGAGCTGTGC CCAGGCTGCT GGGGTGATGA TGCAACCCGA CGACTGGCCC TTTGTTGTCT 
     3661 TCATCACGTG CTTCCGTCAC AAGATTCCCA ATCTGGAGCG TGCTAAGGGA GCCTTGCTGA 
     3721 GCATCACGGC GGGCCAGAAG GTTATCAGCA AACACAAGAA CGGGCGCTTC TACCAGTGTG 
     3781 AGGTGGTCAG ACTCACCACC GAGACCTTCT ACGAAGTCAA CTTTGACGAC GGCTCCTTCA 
     3841 GTGACAACCT TTATCCTGAG GACATAGTGG TAATGTCTGA AAGCGGCTTC TGGCAGCACT 
     3901 TTGGGTCTGG GACATCTTCT TGTTATTGCC GGCTGGATGA CTGTGGTCTC TTTGCCTGCC 
     3961 CCTGGTCAGT TTCCAAACAG AAAGAGCCTT TATTCCCAGG TTCCCTTTCA AGGAAGAGTG 
     4021 GACATGCTGG GGCGCTCAGC TTCCCCGAGG AGTTCAGAGG AGTCAGTGTT CCCTGCAGTC 
     4081 CACTGAAATA TGCTTATATC TCAGACCAAA TTATTTCTAA TAGCATCACG CGTACGCGGC 
     4141 CGCTCGAGCA GAAACTCATC TCAGAAGAGG ATCTGGCAGC AAATGATATC CTGGATTACA 
     4201 AGGATGACGA CGATAAGGTT TAAACGGCCG GCCGCGGTCA TAGCTGTTTC CTGAACAGAT 
     4261 CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA GTGCCTCTCC TGGCCCTGGA AGTTGCCACT 
     4321 CCAGTGCCCA CCAGCCTTGT CCTAATAAAA TTAAGTTGCA TCATTTTGTC TGACTAGGTG 
     4381 TCCTTCTATA ATATTATGGG GTGGAGGGGG GTGGTATGGA GCAAGGGGCA AGTTGGGAAG 
     4441 ACAACCTGTA GGGCCTGCGG GGTCTATTGG GAACCAAGCT GGAGTGCAGT GGCACAATCT 
     4501 TGGCTCACTG CAATCTCCGC CTCCTGGGTT CAAGCGATTC TCCTGCCTCA GCCTCCCGAG 
     4561 TTGTTGGGAT TCCAGGCATG CATGACCAGG CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA 
     4621 CGGGGTTTCA CCATATTGGC CAGGCTGGTC TCCAACTCCT AATCTCAGGT GATCTACCCA 
     4681 CCTTGGCCTC CCAAATTGCT GGGATTACAG GCGTGAACCA CTGCTCCCTT CCCTGTCCTT 
     4741 CTGATTTTAA AATAACTATA CCAGCAGGAG GACGTCCAGA CACAGCATAG GCTACCTGGC 
     4801 CATGCCCAAC CGGTGGGACA TTTGAGTTGC TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG 
     4861 TCCACTCAGT AGATGCCTGT TGAATTGGGT ACGCGGCCAG CGGCGAGCGG TATCAGCTCA 
     4921 CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG 
     4981 AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA 
     5041 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA 
     5101 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC 
     5161 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC 
     5221 GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT 
     5281 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG 
     5341 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG 
     5401 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA 
     5461 CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG 
     5521 AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT 
     5581 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT 
     5641 TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG 
     5701 ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT 
     5761 CTAAAGTATA TATGAGTAAC CTGAGGCTAT GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC 
     5821 GAGCCCTGGG CCTTCACCCG AACTTGGGGG GTGGGGTGGG GAAAAGGAAG AAACGCGGGC 
     5881 GTATTGGCCC CAATGGGGTC TCGGTGGGGT ATCGACAGAG TGCCAGCCCT GGGACCGAAC 
     5941 CCCGCGTTTA TGAACAAACG ACCCAACACC GTGCGTTTTA TTCTGTCTTT TTATTGCCGT 
     6001 CATAGCGCGG GTTCCTTCCG GTATTGTCTC CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG 
     6061 GTGGGCGAAG AACTCCAGCA TGAGATCCCC GCGCTGGAGG ATCATCCAGC CGGCGTCCCG 
     6121 GAAAACGATT CCGAAGCCCA ACCTTTCATA GAAGGCGGCG GTGGAATCGA AATCTCGTGA 
     6181 TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT TTCGAACCCC AGAGTCCCGC TCAGAAGAAC 
     6241 TCGTCAAGAA GGCGATAGAA GGCGATGCGC TGCGAATCGG GAGCGGCGAT ACCGTAAAGC 
     6301 ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA AGCTCTTCAG CAATATCACG GGTAGCCAAC 
     6361 GCTATGTCCT GATAGCGATC CGCCACACCC AGCCGGCCAC AGTCGATGAA TCCAGAAAAG 
     6421 CGGCCATTTT CCACCATGAT ATTCGGCAAG CAGGCATCGC CATGGGTCAC GACGAGATCC 
     6481 TCGCCGTCGG GCATGCTCGC CTTGAGCCTG GCGAACAGTT CGGCTGGCGC GAGCCCCTGA 
     6541 TGCTCTTCGT CCAGATCATC CTGATCGACA AGACCGGCTT CCATCCGAGT ACGTGCTCGC 
     6601 TCGATGCGAT GTTTCGCTTG GTGGTCGAAT GGGCAGGTAG CCGGATCAAG CGTATGCAGC 
     6661 CGCCGCATTG CATCAGCCAT GATGGATACT TTCTCGGCAG GAGCAAGGTG AGATGACAGG 
     6721 AGATCCTGCC CCGGCACTTC GCCCAATAGC AGCCAGTCCC TTCCCGCTTC AGTGACAACG 
     6781 TCGAGCACAG CTGCGCAAGG AACGCCCGTC GTGGCCAGCC ACGATAGCCG CGCTGCCTCG 
     6841 TCTTGCAGTT CATTCAGGGC ACCGGACAGG TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC 
     6901 TGCGCTGACA GCCGGAACAC GGCGGCATCA GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA 
     6961 TAGCCGAATA GCCTCTCCAC CCAAGCGGCC GGAGAACCTG CGTGCAATCC ATCTTGTTCA 
     7021 ATCATGCGAA ACGATCCTCA TCCTGTCTCT TGATCGATCT TTGCAAAAGC CTAGGCCTCC 
     7081 AAAAAAGCCT CCTCACTACT TCTGGAATAG CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC 
     7141 ATAAATAAAA AAAATTAGTC AGCCATGGGG CGGAGAATGG GCGGAACTGG GCGGAGTTAG 
     7201 GGGCGGGATG GGCGGAGTTA GGGGCGGGAC TATGGTTGCT GACTAATTGA GATGCATGCT 
     7261 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCTGGTTG CTGACTAATT 
     7321 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCCTAA 
     7381 CTGACACACA TTCCACAGCT GGTTCTTTCC GCCTCAGGAC TCTTCCTTTT TCAATATTAT 
     7441 TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA 
     7501 AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA CGCGCCCTGT 
     7561 AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC 
     7621 AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC 
     7681 TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG 
     7741 CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA 
     7801 TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC 
     7861 CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT TTGATTTATA AGGGATTTTG 
     7921 CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//