LOCUS       MR211477                7962 bp    DNA     circular  SYN 
DEFINITION  Kif5a (Myc-DDK-tagged) - Mouse kinesin family member 5A (Kif5a), transcript variant 2
ACCESSION   NM_008447
VERSION     NM_008447.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4119..4210 
                     /label="Myc-DDK"  
     misc_feature    4240..4825 
                     /label="PolyA signal"  
     misc_feature    4297..4316 
                     /label="Restriction Enzyme"  
     misc_feature    5101..5773 
                     /label="ColE1"  
     misc_feature    6203..6997 
                     /label="Kan/Neo"  
     misc_feature    7032..7334 
                     /label="SV40 ori"  
     misc_feature    7420..7897 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4101..4107 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4101 
                     /label="Kif5a"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGGAGACT AACAACGAAT GCAGCATCAA GGTGCTTTGC CGATTTCGGC 
     1081 CCCTGAACCA GGCCGAGATT CTGCGGGGGG ACAAGTTCAT CCCCATTTTC CAAGGGGACG 
     1141 ACAGCGTCAT TATTGGGGGA AAGCCATATG TCTTTGACCG CGTCTTCCCC CCAAACACCA 
     1201 CTCAGGAGCA GGTTTACCAC GCCTGTGCCA TGCAGATCGT CAAAGACGTC CTTGCTGGTT 
     1261 ACAATGGCAC AATCTTCGCT TATGGACAGA CATCCTCAGG GAAAACGCAT ACCATGGAGG 
     1321 GGAAGCTGCA CGACCCTCAG CTGATGGGCA TCATTCCCCG GATCGCTCGA GACATCTTCA 
     1381 ACCACATCTA CTCCATGGAT GAGAACCTTG AATTCCACAT TAAGGTATCT TACTTCGAGA 
     1441 TTTACCTGGA TAAGATCCGT GACCTTTTGG ATGTGACCAA GACGAACCTG TCCGTGCATG 
     1501 AGGACAAAAA CCGGGTGCCG TTTGTCAAGG GTTGTACCGA ACGCTTTGTG TCCAGCCCAG 
     1561 AGGAGATTCT GGATGTGATC GATGAGGGGA AGTCCAACCG TCACGTAGCT GTCACCAACA 
     1621 TGAACGAGCA CAGTTCTCGG AGCCACAGCA TCTTCCTCAT CAACATCAAG CAGGAGAACG 
     1681 TAGAGACCGA GCAGAAGCTC AGCGGGAAGC TGTACCTCGT GGATCTGGCC GGAAGCGAGA 
     1741 AGGTCAGCAA GACAGGGGCA GAGGGAGCCG TTCTGGACGA GGCAAAGAAT ATCAACAAGT 
     1801 CGCTGTCGGC CCTGGGGAAC GTGATCTCTG CACTGGCAGA GGGCACCAAA AGCTACGTGC 
     1861 CGTACCGCGA CAGCAAAATG ACGAGGATTC TCCAGGACTC TCTGGGAGGG AACTGCAGGA 
     1921 CTACCATGTT CATCTGCTGC TCGCCGTCCA GCTACAATGA CGCAGAGACC AAGTCCACGC 
     1981 TCATGTTTGG ACAGCGGGCG AAGACCATCA AGAACACTGC CTCAGTGAAT CTGGAGCTGA 
     2041 CTGCTGAGCA GTGGAAGAAG AAGTATGAGA AGGAGAAGGA GAAGACCAAG GCCCAGAAGG 
     2101 AGACAATTGC GAAGCTAGAG GCTGAGCTTA GCCGGTGGCG CAATGGAGAG AATGTGCCTG 
     2161 AGACTGAGCG CCTGGCTGGA GAGGACTCAG CTCTGGGAGC TGAGCTCTGC GAGGAGACCC 
     2221 CTGTGAATGA CAACTCATCC ATTGTGGTAC GCATCGCACC TGAGGAAAGG CAGAAATATG 
     2281 AGGAAGAGAT CCGCCGTCTC TACAAGCAGC TTGATGACAA GGATGATGAG ATCAACCAGC 
     2341 AGAGCCAGCT CATTGAGAAG CTGAAGCAGC AGATGCTGGA CCAGGAAGAG CTGCTCGTGT 
     2401 CCACTCGGGG AGACAACGAG AAGGTCCAGC GGGAGCTTAG CCACCTGCAG TCCGAGAACG 
     2461 ATGCTGCGAA GGACGAGGTG AAGGAAGTGC TGCAGGCCCT AGAGGAGCTG GCGGTCAACT 
     2521 ACGACCAGAA GTCCCAGGAG GTGGAGGAGA AGAGCCAGCA GAACCAGCTG CTGGTGGACG 
     2581 AGCTGTCCCA GAAAGTGGCC ACCATGCTGT CCCTGGAGTC CGAGCTACAG CGGCTCCAGG 
     2641 AGGTCAGTGG ACACCAGCGA AAGCGGATCG CTGAGGTGCT GAATGGGCTG ATGAGGGACC 
     2701 TGAGTGAGTT CAGTGTCATC GTGGGCAACG GCGAGATTAA GCTGCCCGTG GAGATCAGTG 
     2761 GGGCCATCGA GGAGGAGTTC ACGGTGGCCC GGCTCTACAT CAGCAAGATC AAGTCGGAGG 
     2821 TGAAGTCCGT GGTTAAGCGA TGTCGGCAGC TGGAGAACCT CCAGGTGGAG TGTCATCGCA 
     2881 AGATGGAGGT GACCGGTAGG GAGCTGTCAT CTTGCCAACT GCTCATCTCA CAGCATGAGG 
     2941 CCAAGATCCG TTCACTCACG GAGTACATGC AGACTGTGGA GTTGAAGAAA CGGCACCTGG 
     3001 AAGAGTCCTA CGACTCCCTG AGCGATGAGC TTGCCAGGCT CCAGGCGCAC GAAACTGTAC 
     3061 ACGAGGTAGC TCTGAAAGAC AAGGAGCCAG ACACACAGGA CGCGGAGGAG GTGAAGAAGG 
     3121 CCCTGGAACT ACAGATGGAG AATCACCGTG AGGCCCATCA CCGGCAGCTG GCCCGCCTCC 
     3181 GGGATGAGAT TAATGAGAAA CAGAAAACCA TTGATGAGCT GAAAGACCTG AACCAGAAGC 
     3241 TCCAGTTAGA GCTGGAGAAG CTTCAGGCCG ACTATGAGAG GCTGAAGAAT GAAGAGAACG 
     3301 AGAAGAGCGC CAAGCTCCAG GAGCTGACAT TTCTGTATGA GCGACATGAG CAGTCCAAGC 
     3361 AGGACCTCAA GGGGCTGGAG GAGACAGTTG CCCGTGAACT CCAGACCCTC CACAACCTTC 
     3421 GCAAGCTGTT CGTTCAAGAC GTCACGACTC GAGTCAAGAA AAGTGCAGAA ATGGAGCCCG 
     3481 AGGACAGTGG GGGGATTCAT TCCCAAAAGC AGAAGATCTC CTTTCTTGAG AACAACCTGG 
     3541 AACAGCTTAC AAAGGTTCAC AAACAGCTGG TACGTGACAA TGCAGATCTG CGTTGTGAGC 
     3601 TTCCTAAATT GGAAAAACGA CTTCGGGCTA CGGCTGAGAG AGTTAAGGCC CTGGAGGGTG 
     3661 CACTGAAGGA GGCCAAGGAG GGCGCTATGA AGGACAAGCG TAGATACCAG CAGGAGGTGG 
     3721 ACCGCATCAA AGAAGCCGTG CGGTACAAGA GCTCCGGCAA GCGGGGCCAT TCTGCCCAGA 
     3781 TCGCTAAGCC TGTGAGGCCT GGCCACTATC CTGCCTCCTC ACCCACCAAC CCCTACGGCA 
     3841 CCCGGAGCCC CGAGTGTATC AGCTACACCA ACAACCTCTT CCAGAACTAC CAGAACCTGC 
     3901 ACCTGCAGGC TGCGCCTAGC TCCACTTCAG ATATGTACTT TGCCAGCAGC GGAGCCACAT 
     3961 CTGTTGCCCC CTTGGCTTCC TACCAGAAGG CCAACATGGA CAATGGAAAT GCCACAGATA 
     4021 TCAACGACAA CAGGAGTGAC CTGCCGTGTG GCTATGAGGC TGAGGACCAG GCCAAGCTTT 
     4081 TCCCTCTCCA CCAAGAGACA GCAGCCAGCA CGCGTACGCG GCCGCTCGAG CAGAAACTCA 
     4141 TCTCAGAAGA GGATCTGGCA GCAAATGATA TCCTGGATTA CAAGGATGAC GACGATAAGG 
     4201 TTTAAACGGC CGGCCGCGGT CATAGCTGTT TCCTGAACAG ATCCCGGGTG GCATCCCTGT 
     4261 GACCCCTCCC CAGTGCCTCT CCTGGCCCTG GAAGTTGCCA CTCCAGTGCC CACCAGCCTT 
     4321 GTCCTAATAA AATTAAGTTG CATCATTTTG TCTGACTAGG TGTCCTTCTA TAATATTATG 
     4381 GGGTGGAGGG GGGTGGTATG GAGCAAGGGG CAAGTTGGGA AGACAACCTG TAGGGCCTGC 
     4441 GGGGTCTATT GGGAACCAAG CTGGAGTGCA GTGGCACAAT CTTGGCTCAC TGCAATCTCC 
     4501 GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA 
     4561 TGCATGACCA GGCTCAGCTA ATTTTTGTTT TTTTGGTAGA GACGGGGTTT CACCATATTG 
     4621 GCCAGGCTGG TCTCCAACTC CTAATCTCAG GTGATCTACC CACCTTGGCC TCCCAAATTG 
     4681 CTGGGATTAC AGGCGTGAAC CACTGCTCCC TTCCCTGTCC TTCTGATTTT AAAATAACTA 
     4741 TACCAGCAGG AGGACGTCCA GACACAGCAT AGGCTACCTG GCCATGCCCA ACCGGTGGGA 
     4801 CATTTGAGTT GCTTGCTTGG CACTGTCCTC TCATGCGTTG GGTCCACTCA GTAGATGCCT 
     4861 GTTGAATTGG GTACGCGGCC AGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG 
     4921 GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA 
     4981 GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA 
     5041 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG 
     5101 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT 
     5161 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG 
     5221 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC 
     5281 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT 
     5341 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA 
     5401 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC 
     5461 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC 
     5521 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT 
     5581 TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC 
     5641 TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT 
     5701 CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA 
     5761 ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC GACGTTGGCT GCGAGCCCTG GGCCTTCACC 
     5821 CGAACTTGGG GGGTGGGGTG GGGAAAAGGA AGAAACGCGG GCGTATTGGC CCCAATGGGG 
     5881 TCTCGGTGGG GTATCGACAG AGTGCCAGCC CTGGGACCGA ACCCCGCGTT TATGAACAAA 
     5941 CGACCCAACA CCGTGCGTTT TATTCTGTCT TTTTATTGCC GTCATAGCGC GGGTTCCTTC 
     6001 CGGTATTGTC TCCTTCCGTG TTTCAGTTAG CCTCCCCCTA GGGTGGGCGA AGAACTCCAG 
     6061 CATGAGATCC CCGCGCTGGA GGATCATCCA GCCGGCGTCC CGGAAAACGA TTCCGAAGCC 
     6121 CAACCTTTCA TAGAAGGCGG CGGTGGAATC GAAATCTCGT GATGGCAGGT TGGGCGTCGC 
     6181 TTGGTCGGTC ATTTCGAACC CCAGAGTCCC GCTCAGAAGA ACTCGTCAAG AAGGCGATAG 
     6241 AAGGCGATGC GCTGCGAATC GGGAGCGGCG ATACCGTAAA GCACGAGGAA GCGGTCAGCC 
     6301 CATTCGCCGC CAAGCTCTTC AGCAATATCA CGGGTAGCCA ACGCTATGTC CTGATAGCGA 
     6361 TCCGCCACAC CCAGCCGGCC ACAGTCGATG AATCCAGAAA AGCGGCCATT TTCCACCATG 
     6421 ATATTCGGCA AGCAGGCATC GCCATGGGTC ACGACGAGAT CCTCGCCGTC GGGCATGCTC 
     6481 GCCTTGAGCC TGGCGAACAG TTCGGCTGGC GCGAGCCCCT GATGCTCTTC GTCCAGATCA 
     6541 TCCTGATCGA CAAGACCGGC TTCCATCCGA GTACGTGCTC GCTCGATGCG ATGTTTCGCT 
     6601 TGGTGGTCGA ATGGGCAGGT AGCCGGATCA AGCGTATGCA GCCGCCGCAT TGCATCAGCC 
     6661 ATGATGGATA CTTTCTCGGC AGGAGCAAGG TGAGATGACA GGAGATCCTG CCCCGGCACT 
     6721 TCGCCCAATA GCAGCCAGTC CCTTCCCGCT TCAGTGACAA CGTCGAGCAC AGCTGCGCAA 
     6781 GGAACGCCCG TCGTGGCCAG CCACGATAGC CGCGCTGCCT CGTCTTGCAG TTCATTCAGG 
     6841 GCACCGGACA GGTCGGTCTT GACAAAAAGA ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC 
     6901 ACGGCGGCAT CAGAGCAGCC GATTGTCTGT TGTGCCCAGT CATAGCCGAA TAGCCTCTCC 
     6961 ACCCAAGCGG CCGGAGAACC TGCGTGCAAT CCATCTTGTT CAATCATGCG AAACGATCCT 
     7021 CATCCTGTCT CTTGATCGAT CTTTGCAAAA GCCTAGGCCT CCAAAAAAGC CTCCTCACTA 
     7081 CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC CTCGGCCTCT GCATAAATAA AAAAAATTAG 
     7141 TCAGCCATGG GGCGGAGAAT GGGCGGAACT GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT 
     7201 TAGGGGCGGG ACTATGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     7261 TGGGGAGCCT GGGGACTTTC CACACCTGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     7321 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCCT AACTGACACA CATTCCACAG 
     7381 CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT 
     7441 TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT 
     7501 CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGCGCCCT GTAGCGGCGC ATTAAGCGCG 
     7561 GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT 
     7621 CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA 
     7681 AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA 
     7741 CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT 
     7801 TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC 
     7861 AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG 
     7921 TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TT
//