LOCUS       MR211476                7965 bp    DNA     circular  SYN 
DEFINITION  Dpyd (Myc-DDK-tagged) - Mouse dihydropyrimidine dehydrogenase (Dpyd)
ACCESSION   NM_170778
VERSION     NM_170778.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4120..4211 
                     /label="Myc-DDK"  
     misc_feature    4241..4826 
                     /label="PolyA signal"  
     misc_feature    4298..4317 
                     /label="Restriction Enzyme"  
     misc_feature    5102..5774 
                     /label="ColE1"  
     misc_feature    6204..6998 
                     /label="Kan/Neo"  
     misc_feature    7033..7335 
                     /label="SV40 ori"  
     misc_feature    7421..7898 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4095..4102 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4095 
                     /label="Dpyd"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGGGTGTG CTGAGCAGGG ACGCGCCGGA CATCGAGAGT ATCCTGGCTT 
     1081 TAAACCCTCG AGTACAAGCT CATGCAACTC TGCGTTCCAC CGCAGCCAAG AAACTAGACA 
     1141 AGAAACACTG GAAAAGGAAC ACGGACAAGA ACTGCTTTAC CTGTGAGAAG CTGGAGAGTA 
     1201 ATTTTGATGA CATCAAGCAC ACGACTCTTG GTGAGCGAGG AGCTCTCCGA GAAGCAGTGA 
     1261 GATGCCTGAA GTGCGCAGAT GCTCCCTGCC AGAAGAGCTG TCCCACATCT CTTGACATTA 
     1321 AATCATTTAT CACAAGTATC GCCAACAAGA ACTACTATGG TGCAGCTAAG CTGATTTTTT 
     1381 CTGATAACCC TCTTGGTCTT ACTTGTGGAA TGGTTTGTCC AACATCTGAC CTCTGTGTTG 
     1441 GAGGATGCAA CTTACATGCT GCTGAAGAGG GACCAATTAA TATTGGTGGG CTGCAGCAGT 
     1501 TTGCTACTGA GGTGTTCAAA GCAATGAACA TCCCACAGAT CAGAAACCCA TCGCTGCCTC 
     1561 CTCCGGAACA TATGCCGGAA GCCTACTCAG CAAAAATTGC ACTTTTTGGT GCAGGGCCTG 
     1621 CGAGTATAAG CTGTGCCTCC TTTTTGGCTC GGTTGGGCTA TTCCAACATT ACTATATTCG 
     1681 AAAAACAAGA ATATGTTGGT GGTTTAAGCA CTTCTGAAAT CCCTCAGTTT CGACTCCCAT 
     1741 ATGACGTCGT GAATTTTGAG ATTGAGCTCA TGAAGGACCT TGGTGTAAAG ATAATTTGTG 
     1801 GTAAAAGCCT TTCCACCGAT GAAATGACTC TTAGCTCTTT GAAAGAAAAT GGCTACAGAG 
     1861 CTGCATTTAT TGGAATAGGC TTGCCAGAAC CCAAAAAGGA CCATATTTTC CAAGGCTTGA 
     1921 CACAAGTCCA GGGATTTTAC ACATCTAAAG ACTTTCTGCC ACTTGTCGCC AAAAGCAGTA 
     1981 AAACAGGAAT GTGCGCCTGT CACTCTCCAT TGCCATCCAT AAGGGGAGCC GTGATTGTAC 
     2041 TCGGAGCTGG AGATACTGCG TTTGACTGTG CAACATCCGC TCTGCGCTGT GGAGCCCTTC 
     2101 GCGTGTTCAT CGTCTTCAGA AAGGGCTTTG TTAATATTCG AGCTGTTCCA GAGGAGATGG 
     2161 AGCTCGCTAA GGAAGAGAAA TGTGAATTTT TGCCTTTCCT CTCGCCACGG AAGGTTATAG 
     2221 TCAAAGATGG AAAGATTGTG GCAATGCAAT TTGTTCGAAC TGAGCAAGAT GAAACCGGAA 
     2281 ACTGGGTGGA AGATGAAGAG CAGACGGTGC GGCTAAAGGC TGATGTGGTT ATTAGTGCCT 
     2341 TTGGATCTGT CCTGGAGGAT CCCAAAGTGA AAGAAGCATT GAGTCCCATC AAGTTTAACA 
     2401 GATGGGGTCT CCCAGAAGTA AATCCAGAAA CCATGCAAAC CAGTGAACCA TGGGTGTTTG 
     2461 CAGGTGGTGA TGTTGTGGGT ATGGCTAACA CCACAGTGGA ATCTGTCAAC GATGGAAAGC 
     2521 AAGCTTCATG GTACATTCAC AAGCACATAC AGGCACAATA TGGAACCTCA GTGCCTTCTC 
     2581 AGCCTACAAT GCCCCTATTT TACACTCCGG TTGACTTAGT GGACATCAGC GTGGAAATGG 
     2641 CAGGGTTGAG GTTTCCCAAT CCCTTTGGCC TTGCCAGTGC GACACCAGCC ACTAGCACAC 
     2701 CAATGATTCG AAGGGCCTTT GAAGCTGGCT GGGGTTTTGC TCTGACCAAA ACTTTCTCTC 
     2761 TTGATAAGGA CATCGTGACA AACGTCTCAC CAAGAATCAT CCGGGGGACA ACTTCTGGCC 
     2821 CCTTGTATGG CCCTGGACAA AGCTCCTTTC TTAACATTGA GCTCATCAGT GAGAAAACAG 
     2881 CTGCATATTG GTGTCACAGT GTCACCGAAC TAAAGGCTGA CTTCCCGGAC AATATCCTGA 
     2941 TCGCCAGCAT CATGTGCAGT TACAACAAGA GTGACTGGAT GGAACTCTCC AAAATGGCTG 
     3001 AGGCTTCTGG AGCAGATGCC CTGGAGTTAA ATTTATCGTG TCCACATGGC ATGGGGGAGA 
     3061 GAGGAATGGG CCTGGCTTGT GGGCAGGATC CAGAGCTGGT GAGGAACATC TGTCGCTGGG 
     3121 TTAGGCAAGC TGTTCGGGTT CCATTTTTTG CCAAGTTGAC CCCAAATGTC ACTGATATTG 
     3181 TAAGCATCGC AAGAGCAGCA AAGGAAGGTG GAGCAGATGG TGTAACAGCC ACCAACACCG 
     3241 TCTCGGGTCT GATGGGACTG AAAGCTGATG GCACACCCTG GCCTGCTGTG GGCATTGGAA 
     3301 GGAGGACTAC ATATGGAGGA GTGTCAGGAA CTGCTATCAG GCCCATTGCT TTGAGAGCTG 
     3361 TGACCGCCAT TGCCCGTGCT TTGCCTGGGT TTCCTATTTT GGCCACAGGT GGAATTGACT 
     3421 CAGCTGAAAG TGGACTTCAG TTTCTTCATA GTGGTGCTTC AGTTCTTCAG GTATGCAGCG 
     3481 CTATTCAGAA TCAGGACTTC ACTGTGATTG AAGATTACTG CACTGGACTC AAAGCCCTGC 
     3541 TTTATCTGAA GAGCATTGAA GAGTTAGCAG ACTGGGATGG TCAGAGTCCA CCCATTATAA 
     3601 GTCATCAGAA AGGGAAACCA GTTCCACGCG TTGCTGAGCT CATGGGACAG AAACTGCCCA 
     3661 GCTTTGGACC TTACCTTGAA CAGCGCAAGA AAATCATAGC AGCAAGCAAA ATCAGACAGA 
     3721 AAGATCAAAA CACAGCTTGC TCACCTCTCC AGAGAAAGCA CTTTAACTCG CAAAAGCCTA 
     3781 TTCCTGCCAT CAAGGATGTA ATTGGAAAAT CACTGCAATA CCTGGGAACA TTTGGTGAGA 
     3841 TGAGCATCAT GGAACAAGTT GTGGCCCTGA TCGATGAGGA AATGTGCATC AATTGCGGCA 
     3901 AATGTTACAT GACCTGTAAT GACTCTGGCT ACCAGGCTAT CCAGTTCGAT CCAGAAACTC 
     3961 ACCTGCCTAC CGTTAGCGAC ACATGTACAG GCTGTACTCT CTGCCTCAGC GTCTGCCCTA 
     4021 TTATGGACTG TATCAGGATG GTTTCCAGGG CAACACCTTA TCAACCAAAG AGAGGCCTAC 
     4081 CATTAGCCGT GAAGCCGGTG TGTAGCGGAC CGACGCGTAC GCGGCCGCTC GAGCAGAAAC 
     4141 TCATCTCAGA AGAGGATCTG GCAGCAAATG ATATCCTGGA TTACAAGGAT GACGACGATA 
     4201 AGGTTTAAAC GGCCGGCCGC GGTCATAGCT GTTTCCTGAA CAGATCCCGG GTGGCATCCC 
     4261 TGTGACCCCT CCCCAGTGCC TCTCCTGGCC CTGGAAGTTG CCACTCCAGT GCCCACCAGC 
     4321 CTTGTCCTAA TAAAATTAAG TTGCATCATT TTGTCTGACT AGGTGTCCTT CTATAATATT 
     4381 ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG GGGCAAGTTG GGAAGACAAC CTGTAGGGCC 
     4441 TGCGGGGTCT ATTGGGAACC AAGCTGGAGT GCAGTGGCAC AATCTTGGCT CACTGCAATC 
     4501 TCCGCCTCCT GGGTTCAAGC GATTCTCCTG CCTCAGCCTC CCGAGTTGTT GGGATTCCAG 
     4561 GCATGCATGA CCAGGCTCAG CTAATTTTTG TTTTTTTGGT AGAGACGGGG TTTCACCATA 
     4621 TTGGCCAGGC TGGTCTCCAA CTCCTAATCT CAGGTGATCT ACCCACCTTG GCCTCCCAAA 
     4681 TTGCTGGGAT TACAGGCGTG AACCACTGCT CCCTTCCCTG TCCTTCTGAT TTTAAAATAA 
     4741 CTATACCAGC AGGAGGACGT CCAGACACAG CATAGGCTAC CTGGCCATGC CCAACCGGTG 
     4801 GGACATTTGA GTTGCTTGCT TGGCACTGTC CTCTCATGCG TTGGGTCCAC TCAGTAGATG 
     4861 CCTGTTGAAT TGGGTACGCG GCCAGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT 
     4921 ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA 
     4981 AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC 
     5041 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA 
     5101 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC 
     5161 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC 
     5221 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA 
     5281 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC 
     5341 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG 
     5401 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG 
     5461 AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG 
     5521 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA 
     5581 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA 
     5641 CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT 
     5701 CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA 
     5761 GTAACCTGAG GCTATGGCAG GGCCTGCCGC CCCGACGTTG GCTGCGAGCC CTGGGCCTTC 
     5821 ACCCGAACTT GGGGGGTGGG GTGGGGAAAA GGAAGAAACG CGGGCGTATT GGCCCCAATG 
     5881 GGGTCTCGGT GGGGTATCGA CAGAGTGCCA GCCCTGGGAC CGAACCCCGC GTTTATGAAC 
     5941 AAACGACCCA ACACCGTGCG TTTTATTCTG TCTTTTTATT GCCGTCATAG CGCGGGTTCC 
     6001 TTCCGGTATT GTCTCCTTCC GTGTTTCAGT TAGCCTCCCC CTAGGGTGGG CGAAGAACTC 
     6061 CAGCATGAGA TCCCCGCGCT GGAGGATCAT CCAGCCGGCG TCCCGGAAAA CGATTCCGAA 
     6121 GCCCAACCTT TCATAGAAGG CGGCGGTGGA ATCGAAATCT CGTGATGGCA GGTTGGGCGT 
     6181 CGCTTGGTCG GTCATTTCGA ACCCCAGAGT CCCGCTCAGA AGAACTCGTC AAGAAGGCGA 
     6241 TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG GCGATACCGT AAAGCACGAG GAAGCGGTCA 
     6301 GCCCATTCGC CGCCAAGCTC TTCAGCAATA TCACGGGTAG CCAACGCTAT GTCCTGATAG 
     6361 CGATCCGCCA CACCCAGCCG GCCACAGTCG ATGAATCCAG AAAAGCGGCC ATTTTCCACC 
     6421 ATGATATTCG GCAAGCAGGC ATCGCCATGG GTCACGACGA GATCCTCGCC GTCGGGCATG 
     6481 CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT GGCGCGAGCC CCTGATGCTC TTCGTCCAGA 
     6541 TCATCCTGAT CGACAAGACC GGCTTCCATC CGAGTACGTG CTCGCTCGAT GCGATGTTTC 
     6601 GCTTGGTGGT CGAATGGGCA GGTAGCCGGA TCAAGCGTAT GCAGCCGCCG CATTGCATCA 
     6661 GCCATGATGG ATACTTTCTC GGCAGGAGCA AGGTGAGATG ACAGGAGATC CTGCCCCGGC 
     6721 ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC GCTTCAGTGA CAACGTCGAG CACAGCTGCG 
     6781 CAAGGAACGC CCGTCGTGGC CAGCCACGAT AGCCGCGCTG CCTCGTCTTG CAGTTCATTC 
     6841 AGGGCACCGG ACAGGTCGGT CTTGACAAAA AGAACCGGGC GCCCCTGCGC TGACAGCCGG 
     6901 AACACGGCGG CATCAGAGCA GCCGATTGTC TGTTGTGCCC AGTCATAGCC GAATAGCCTC 
     6961 TCCACCCAAG CGGCCGGAGA ACCTGCGTGC AATCCATCTT GTTCAATCAT GCGAAACGAT 
     7021 CCTCATCCTG TCTCTTGATC GATCTTTGCA AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA 
     7081 CTACTTCTGG AATAGCTCAG AGGCCGAGGC GGCCTCGGCC TCTGCATAAA TAAAAAAAAT 
     7141 TAGTCAGCCA TGGGGCGGAG AATGGGCGGA ACTGGGCGGA GTTAGGGGCG GGATGGGCGG 
     7201 AGTTAGGGGC GGGACTATGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     7261 TGCTGGGGAG CCTGGGGACT TTCCACACCT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     7321 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CCTAACTGAC ACACATTCCA 
     7381 CAGCTGGTTC TTTCCGCCTC AGGACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG 
     7441 GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG 
     7501 GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGCGC CCTGTAGCGG CGCATTAAGC 
     7561 GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC 
     7621 GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT 
     7681 CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA 
     7741 AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC 
     7801 CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA 
     7861 CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT 
     7921 TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTT
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