LOCUS       MR211475                7956 bp    DNA     circular  SYN 
DEFINITION  Trhde (Myc-DDK-tagged) - Mouse TRH-degrading enzyme (Trhde)
ACCESSION   NM_146241
VERSION     NM_146241.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4113..4204 
                     /label="Myc-DDK"  
     misc_feature    4234..4819 
                     /label="PolyA signal"  
     misc_feature    4291..4310 
                     /label="Restriction Enzyme"  
     misc_feature    5095..5767 
                     /label="ColE1"  
     misc_feature    6197..6991 
                     /label="Kan/Neo"  
     misc_feature    7026..7328 
                     /label="SV40 ori"  
     misc_feature    7414..7891 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4095..4101 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4095 
                     /label="Trhde"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGAGAAGAC GACGCCGCGC TCCGGGCAAG CGGCAGGGGG CTCTCGGACC 
     1081 CGTGGGCTGA CTCGGTGGGA GTGAGACCCC GCACCACGGA GCGCCACATC GCAGTGCACA 
     1141 AGAGGCTTGT GCTGGCCTTT GCCGTGTCCA TCGTGGCACT GCTGGCTGTC ACCATGCTGG 
     1201 CCGTCCTGCT CAGCTTGCGA TTCGACGAGT GTGGAGCGAG CGCGGCGATG CCTGGCACCG 
     1261 ACGGTGGCCT CGGGGGTTTC CCCGAGCGCG ACAGCAACAG CAGCTTTCCA GGTTCTGCCC 
     1321 GGCGCAACCA CCACGCGGGT GGGGAATCCT CTCAGCGTGA GAGCGGCGAG GTGGGCACCC 
     1381 CGGGGACCCC GTCTGCCCAG CCGCCGTCGG AGGAAGAGCG GGAGCAGTGG CAGCCCTGGA 
     1441 CACAGCTGCG CCTATCCGGC CACCTCAAGC CGCTGCACTA CAATTTGATG CTCACCGCTT 
     1501 TCATGGAGAA CTTCACCTTC TCTGGGGAGG TCAACGTGGA GATCGCGTGC CGGAACGCCA 
     1561 CCCGCTACGT GGTACTGCAC GCCTCCCGAG TGGCGGTGGA GAAGGTGCAA GTAGCTGAAG 
     1621 ACAGGGCGTT CGGGGCTGTC CCGGTTGCAG GCTTTTTCCT CTACCCACAA ACGCAGGTCT 
     1681 TGGTGGTGGT ATTGAACAGA ACCCTGGACG CGCAGAGGCA TTACAATCTG AAGATTATCT 
     1741 ACAATGCCCT GATAGAGAAC GAGCTTTTGG GCTTCTTCCG CAGCTCCTAC GTGATCCACG 
     1801 GGGAGAGAAG ATTCCTTGGT GTTACTCAGT TTTCACCTAC ACATGCCAGG AAGGCGTTTC 
     1861 CGTGTTTCGA TGAACCAATC TACAAGGCCA CTTTCAAAAT CAGCATCAAA CATCAAGCAA 
     1921 CCTATTTGTC TCTATCCAAC ATGCCAGTGG AAACGTCTGT GTTCGAGGAA GATGGATGGG 
     1981 TGACAGATCA CTTTTCACAG ACCCCTCTCA TGTCTACATA TTATTTAGCC TGGGCGATTT 
     2041 GCAACTTCAC ATACAGAGAA ACTACCACCA AGAGTGGGGT TGTAGTCCGA TTATATGCGA 
     2101 GACCCGACGC TATCAGAAGA GGATCCGGGG ACTATGCTCT CCACATTACA AAGAGATTAA 
     2161 TAGAATTTTA TGAAGACTAC TTTAAAGTGC CCTATTCTTT GCCAAAACTA GATCTTTTAG 
     2221 CTGTGCCTAA GCATCCTTAT GCTGCTATGG AGAACTGGGG ACTAAGTATT TTTGTGGAAC 
     2281 AAAGAATACT GCTGGATCCC AGTGTGTCAT CTATTTCTTA TTTGCTGGAT GTCACCATGG 
     2341 TCATTGTTCA TGAAATATGT CACCAGTGGT TTGGTGACCT TGTGACTCCA GTTTGGTGGG 
     2401 AAGATGTGTG GTTGAAGGAA GGCTTTGCTC ACTACTTTGA ATTTGTGGGT ACAGACTACC 
     2461 TCTACCCTGC CTGGAACATG GAGAAGCAGA GATTTCTGAC AGATGTTCTG CATGAAGTGA 
     2521 TGCTACTTGA TGGCTTGGCC AGTTCCCATC CAGTATCACA GGAAGTGCTA CGGGCAACAG 
     2581 ATATTGACAG AGTGTTCGAC TGGATCGCAT ACAAAAAGGG TGCTGCTCTA ATTAGAATGC 
     2641 TGGCTAATTT TATGGGTCAT TCAGTTTTTC AGAGGGGATT GCAAGATTAT TTAACCATTC 
     2701 ACAAGTATGG CAATGCAGCC AGAAATGATC TCTGGAATAC ATTATCAGAG GCTTTAAGAA 
     2761 GGAATGGAAA ATACGTGAAC ATACAAGAAG TAATGGATCA GTGGACACTG CAGATGGGAT 
     2821 ATCCTGTTAT CACTATCCTG GGAAACACGA CGGCAGAAAA TAGAATACTT ATCACCCAAC 
     2881 AGCATTTCAT TTATGACATT GGTGCTAAAA CTAAAGCACT TCAACTTCAA AACAGCAGTT 
     2941 ACCTGTGGCA GATTCCATTA ACCATTGTGG TAGGAAATAG AAGCCATGTG TCTTCAGAAG 
     3001 CAATTATTTG GGTGTCTAAC AAATCAGAAC ATCACAGAAT AGCGTACTTA GACAGAGGAA 
     3061 GCTGGATCCT TGGGAACATC AATCAAACCG GCTACTTTAG AGTCAACTAT GACCTAAGGA 
     3121 ACTGGAGATT ACTAATCGAT CAGTTAATCA GGAACCACGA GGTACTTTCT GTCAGTAACC 
     3181 GCGCGGCTTT GATCGATGAT GCCTTCAGCC TGGCCAGGGC AGGCTATTTG CCTCAGAATA 
     3241 TCCCCCTAGA GATCATCAGA TACCTGTCTG AGGAAAAGGA TTTTCTTCCT TGGCACGCTG 
     3301 CCAGCCGAGC TCTTTATCCT CTGGATAAAT TACTGGACCG CATGGAGAAC TACAATATCT 
     3361 TCAATGAATA TATTCTAAAA CAAGTTGCAA CAACATACAT CAAGCTTGGA TGGCCAAGAA 
     3421 ATAACTTCAA TGGATCTCTC GTTCAAGCCT CCTACCAACA TGAGGAGCTA CGGAGGGAAG 
     3481 TGATAATGTT GGCATGTAGT TTTGGGAACA AGCACTGTCA CCAGCAGGCA TCAACACTTA 
     3541 TTTCAGACTG GATTTCCAGC AACAGAAACA GAATACCCCT GAATGTTAGA GACATCGTCT 
     3601 ACTGCACAGG AGTGTCACTG CTGGATGAGG ATGTCTGGGA GTTTATCTGG ATGAAATTCC 
     3661 ACTCTACCAC TGCAGTTTCT GAGAAAAAGA TACTGCTGGA GGCTCTAACT TGCAGCGATG 
     3721 ACAGGAATTT ATTAAGTAGG CTTCTAAATC TGTCTCTGAA TTCTGAGGTG GTGTTGGATC 
     3781 AAGATGCAAT TGATGTAATT ATCCACGTAG CCAGAAATCC ACATGGCCGA GACCTTGCCT 
     3841 GGAAGTTTTT CAGAGACAAA TGGAAAATAC TAAATACCAG GTATGGAGAA GCACTGTTTA 
     3901 TGAATTCCAA ACTTATCAGT GGAGTTACAG AATTTCTTAA TACAGAAGGT GAACTTAAAG 
     3961 AGCTAAAGAA TTTCATGAAG TCCTATGATG GGGTAGCCTC TGCTTCTTTC TCACGAGCTG 
     4021 TGGAAACTGT GGAAGCCAAT GTGCGCTGGA AAAGGTTTTA CCAAGATGAG CTTTTCCAGT 
     4081 GGCTGGGAAA AGCCATGAGA CACACGCGTA CGCGGCCGCT CGAGCAGAAA CTCATCTCAG 
     4141 AAGAGGATCT GGCAGCAAAT GATATCCTGG ATTACAAGGA TGACGACGAT AAGGTTTAAA 
     4201 CGGCCGGCCG CGGTCATAGC TGTTTCCTGA ACAGATCCCG GGTGGCATCC CTGTGACCCC 
     4261 TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT GCCACTCCAG TGCCCACCAG CCTTGTCCTA 
     4321 ATAAAATTAA GTTGCATCAT TTTGTCTGAC TAGGTGTCCT TCTATAATAT TATGGGGTGG 
     4381 AGGGGGGTGG TATGGAGCAA GGGGCAAGTT GGGAAGACAA CCTGTAGGGC CTGCGGGGTC 
     4441 TATTGGGAAC CAAGCTGGAG TGCAGTGGCA CAATCTTGGC TCACTGCAAT CTCCGCCTCC 
     4501 TGGGTTCAAG CGATTCTCCT GCCTCAGCCT CCCGAGTTGT TGGGATTCCA GGCATGCATG 
     4561 ACCAGGCTCA GCTAATTTTT GTTTTTTTGG TAGAGACGGG GTTTCACCAT ATTGGCCAGG 
     4621 CTGGTCTCCA ACTCCTAATC TCAGGTGATC TACCCACCTT GGCCTCCCAA ATTGCTGGGA 
     4681 TTACAGGCGT GAACCACTGC TCCCTTCCCT GTCCTTCTGA TTTTAAAATA ACTATACCAG 
     4741 CAGGAGGACG TCCAGACACA GCATAGGCTA CCTGGCCATG CCCAACCGGT GGGACATTTG 
     4801 AGTTGCTTGC TTGGCACTGT CCTCTCATGC GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA 
     4861 TTGGGTACGC GGCCAGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC 
     4921 CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG 
     4981 GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA 
     5041 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA 
     5101 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG 
     5161 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG 
     5221 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT 
     5281 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA 
     5341 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG 
     5401 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT 
     5461 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC 
     5521 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG 
     5581 CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG 
     5641 GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA 
     5701 GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAACCTGA 
     5761 GGCTATGGCA GGGCCTGCCG CCCCGACGTT GGCTGCGAGC CCTGGGCCTT CACCCGAACT 
     5821 TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG 
     5881 TGGGGTATCG ACAGAGTGCC AGCCCTGGGA CCGAACCCCG CGTTTATGAA CAAACGACCC 
     5941 AACACCGTGC GTTTTATTCT GTCTTTTTAT TGCCGTCATA GCGCGGGTTC CTTCCGGTAT 
     6001 TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC CCTAGGGTGG GCGAAGAACT CCAGCATGAG 
     6061 ATCCCCGCGC TGGAGGATCA TCCAGCCGGC GTCCCGGAAA ACGATTCCGA AGCCCAACCT 
     6121 TTCATAGAAG GCGGCGGTGG AATCGAAATC TCGTGATGGC AGGTTGGGCG TCGCTTGGTC 
     6181 GGTCATTTCG AACCCCAGAG TCCCGCTCAG AAGAACTCGT CAAGAAGGCG ATAGAAGGCG 
     6241 ATGCGCTGCG AATCGGGAGC GGCGATACCG TAAAGCACGA GGAAGCGGTC AGCCCATTCG 
     6301 CCGCCAAGCT CTTCAGCAAT ATCACGGGTA GCCAACGCTA TGTCCTGATA GCGATCCGCC 
     6361 ACACCCAGCC GGCCACAGTC GATGAATCCA GAAAAGCGGC CATTTTCCAC CATGATATTC 
     6421 GGCAAGCAGG CATCGCCATG GGTCACGACG AGATCCTCGC CGTCGGGCAT GCTCGCCTTG 
     6481 AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC CCCTGATGCT CTTCGTCCAG ATCATCCTGA 
     6541 TCGACAAGAC CGGCTTCCAT CCGAGTACGT GCTCGCTCGA TGCGATGTTT CGCTTGGTGG 
     6601 TCGAATGGGC AGGTAGCCGG ATCAAGCGTA TGCAGCCGCC GCATTGCATC AGCCATGATG 
     6661 GATACTTTCT CGGCAGGAGC AAGGTGAGAT GACAGGAGAT CCTGCCCCGG CACTTCGCCC 
     6721 AATAGCAGCC AGTCCCTTCC CGCTTCAGTG ACAACGTCGA GCACAGCTGC GCAAGGAACG 
     6781 CCCGTCGTGG CCAGCCACGA TAGCCGCGCT GCCTCGTCTT GCAGTTCATT CAGGGCACCG 
     6841 GACAGGTCGG TCTTGACAAA AAGAACCGGG CGCCCCTGCG CTGACAGCCG GAACACGGCG 
     6901 GCATCAGAGC AGCCGATTGT CTGTTGTGCC CAGTCATAGC CGAATAGCCT CTCCACCCAA 
     6961 GCGGCCGGAG AACCTGCGTG CAATCCATCT TGTTCAATCA TGCGAAACGA TCCTCATCCT 
     7021 GTCTCTTGAT CGATCTTTGC AAAAGCCTAG GCCTCCAAAA AAGCCTCCTC ACTACTTCTG 
     7081 GAATAGCTCA GAGGCCGAGG CGGCCTCGGC CTCTGCATAA ATAAAAAAAA TTAGTCAGCC 
     7141 ATGGGGCGGA GAATGGGCGG AACTGGGCGG AGTTAGGGGC GGGATGGGCG GAGTTAGGGG 
     7201 CGGGACTATG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     7261 GCCTGGGGAC TTTCCACACC TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     7321 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCCTAACTGA CACACATTCC ACAGCTGGTT 
     7381 CTTTCCGCCT CAGGACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT 
     7441 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC 
     7501 ACATTTCCCC GAAAAGTGCC ACCTGACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT 
     7561 GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC 
     7621 GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG 
     7681 GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT 
     7741 TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG 
     7801 TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT 
     7861 ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA 
     7921 AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTT
//