LOCUS       MR211458                7935 bp    DNA     circular  SYN 
DEFINITION  Man2b2 (Myc-DDK-tagged) - Mouse mannosidase 2, alpha B2 (Man2b2)
ACCESSION   NM_008550
VERSION     NM_008550.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4092..4183 
                     /label="Myc-DDK"  
     misc_feature    4213..4798 
                     /label="PolyA signal"  
     misc_feature    4270..4289 
                     /label="Restriction Enzyme"  
     misc_feature    5074..5746 
                     /label="ColE1"  
     misc_feature    6176..6970 
                     /label="Kan/Neo"  
     misc_feature    7005..7307 
                     /label="SV40 ori"  
     misc_feature    7393..7870 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4074..4080 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4074 
                     /label="Man2b2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGGCCGCTG CGCTGGCTGC CGCTACTCGG CCAGCTGTTG CTGTTGTGGC 
     1081 CGCGGGCAGC TCAGCCCGCC GGCCCGATCC GGGCCTTCGT GGTGCCCCAC AGTCACATGG 
     1141 ACGTGGGCTG GGTCTTCACG GTCCAGGAAA GCATGAGAGC CTATGCAGCC AATGTCTACA 
     1201 CTACCGTGGT GGCGGAACTG GTCCGCGGAG GGCAGCGGCG CTTCATCGCC GTGGAGCAGG 
     1261 AGTTCTTCCG GCTGTGGTGG GACGGTGTGG CCTCTGAGCA GCAGAAACAG CAGGTCCGCC 
     1321 AGCTCCTGCA TGAAGGACGC CTGGAGTTTG TTCTCGGAGG CCAAGTCATG CATGATGAGG 
     1381 CTGTGACCCA CCTGGATGAC CAGATCTTAC AGCTCACAGA AGGACATGGC TTTCTCTATG 
     1441 AAACGTTTGG AATCCGGCCA CAGTTCTCCT GGCACGTGGA CCCTTTCGGT GCATCAGCAA 
     1501 CGACCCCTAC CCTGTTTGCC CTGGCAGGCT TCAATGCCCA TCTCATCTCT CGTATTGACT 
     1561 ACGACCTCAA GGATGCCATG CAGGAAGCTC AGATGCTGCA GTTCGTGTGG CATGGGTCCC 
     1621 CATCCCTGTC AGGGCAGCAG GAAATCTTCA CTCACGTCAT GGACCATTAT AGCTACTGCA 
     1681 CCCCCTCACA CATCCCCTTT TCTAACAGGT CTGGATTTTA CTGGAATGGT GTGGCCGTCT 
     1741 TCCCAGAGCC ACCCCCAGAC GGCGTGTATC CTAACATGAG CGAGCCTGTC ACCGGAGCCA 
     1801 ACATCCACCT CTACGCTGAG GCTCTGGTGG CCAACGTTAA ACAGAGGGCT GCGTGGTTCC 
     1861 GGACGCCCCA CGTCCTCTGG CCCTGGGGAT GTGACAAGCA GTTCTTCAAT GCCTCGGTGC 
     1921 AGTTTGACAA CATGGATCCC CTGCTGGACT ACATCAACCA GCGCACGGCC CAGTTTGGCA 
     1981 TCTCGGTGCA GTACGCCACA CTGAACGACT ACTTCCAAGC CCTGCACGCC ACCAACATGA 
     2041 CCTGGGGCAT CCGTGACCAC CAGGACTTCC TTCCCTATTC TTCAGAACCA CTTCAGGCCT 
     2101 GGACCGGCTT CTACACATCC CGGAGCACCC TCAAGGGTCT AGCGAGACAA GCCAGTGCCT 
     2161 TGCTCTATGC TGGGGAGTCC ATGTTCACAC GCTACATGTG GCCAGACCCC AGTGGGACTC 
     2221 TGGACCCTAC CTGGGCCCTG CAGCAGCTCC AACAGCTTCG CTGGGCTGTG TCTGAGGTCC 
     2281 AGCACCACGA TGCCATCACA GGCACTGAGT CCCCCAAGGT GAAGAACATG TACACGGAAC 
     2341 ATCTCAGGAT GGGGATGCTG GGCGTGCGCA AGCTGATGGT CTCCATTGCC CTGGGCGGGC 
     2401 CCCCAGGGTC TGGCACAGGA GCCCCCAAAG ACATCATGGG GCCTCAAGTG ACACCTGTCC 
     2461 TCTCGGTAGA CACAAGGCCT GTAGGATACT CCGCCTCTGT GTACAACCCG CTGGCCTGGA 
     2521 AGATCACCAC CATTATCACC CTGACTGTCG CTTTCCCCAA TGTCAGCGTC ACCGATGAGT 
     2581 TGGGCCACCC GGTGTCAACA CAGATTCAGA ACTCTACAAA GGACCCTTCT GCATACGACC 
     2641 TGCTCATCCT GACCACCATC CCCGGCCTCA ACTATCGGCA CTATCAAGTC ATGCACGCCC 
     2701 GCGGGGACCA GGCAGGCACC AGGGAGCTGG TCGCCCCCAG GGCCAACACC CTCAAGTTTA 
     2761 GTCTCAAACT GAGGAACCAG CCCAGCCAGG AAGGCAAGCG CCTGGTGCCC GTGATGAATG 
     2821 ACTGCTATAT TCTGCTGTTC GACCAGGACA CCAACATGTT ACATAGCATT CAGGATAGAC 
     2881 AGAGCAACCG CACGGTGCGC ATGACCCAAG AGTTCCTGGA GTACCAGGCC AACTGGGACG 
     2941 TGAAGCAGGG CCCCATTTCT GACAACTACC TCTTTGCACC CAACAACACC GCTGAGCCTT 
     3001 CCTGGGAAGC GGTGGGGATG GAAATGGTGG CAGGCACACT GGTAACGGAC ATAAGGCAGT 
     3061 ATTTCTACAG GTACATAACA GACCAGGAGT ACATCTATTC CATCCACACC CGTCTGGCCC 
     3121 ACCCGAGCCT CGCTGGAGAA CTGCTCTGCC AACGGATAGA GCAGCAGTAC CGAGTGGGCC 
     3181 CCCTGGACCT AAACCGTGAG GCCATCCTGA GGACCAGCAG CGACCTAAAC AGCCAGCAAG 
     3241 TCCTCTACTC TGACAACAAC GGCTACCAGA TGCAGCGGAG GCCCTACAAG GCTTTCAAGA 
     3301 GCAATCCCAT CCCCCGGAAT TACTACCCCA TGGTACAGTC AGCCTTCATC GAGGATGACA 
     3361 AGAGCAGGCT GGTGCTGCTG GCAGAGCGAC CGCACGGTGT CTCCAGCCAA GGGAATGGGC 
     3421 AGGTGGAGGT GATGCTCCAT CGACGGCTGT GGAACAACCT GGCCTGGGAC CTGAAGTACA 
     3481 ACCTCACGCT GAACGACACC TCCATTGTCC ACCCGGTGCT CTGGCTCATG CTGGGGCCCA 
     3541 AGTCCACCAT GACGGCCCTG CACCCAAGGA GTGGGGTGGC TCTGCAGCAC GGGCCCGTTG 
     3601 TGTTGTTAAA AGAACTGGCT GACGAAGAAA CGCCCGTTCA CGGACCCCAC AATCCCTGGC 
     3661 CTGTGACACT GCCCCCAAAC CTGCATCTGC AGATCCTCAG CGTACCTGGC TGGACGTACA 
     3721 GCAGGAGCCA TGCCCAGCAC CTGAGGAACC TTCAGAGAGG CCACCCGGAG AAGCCACAGG 
     3781 CCAACCTGCA AAGGGTGCTG CTGCGCCTGC GCCACCTGTA TGAAGCAGGG GAGGACCCAG 
     3841 TACTGTCTCG GCCTGCGACA GTGGATCTCA AGGTTGTACT TCGAGGACTA GGGTCTGTAG 
     3901 TGGCTGTGGA GGAACGCTCA CTCACAGGGA CCTGGGATGT GCAGATGCTG CAGCGCTGGC 
     3961 ACTGGAGCAC AAAGACTGAC CACCTGAAAG GCCACCCCAC CTCTCCACCA AGGCCACCAG 
     4021 GAGGCTCCAT CATCACCGTG TACCCTAAGG AAATCCGGAC CTTCTTCATT AAATTCCAGC 
     4081 AGACGCGTAC GCGGCCGCTC GAGCAGAAAC TCATCTCAGA AGAGGATCTG GCAGCAAATG 
     4141 ATATCCTGGA TTACAAGGAT GACGACGATA AGGTTTAAAC GGCCGGCCGC GGTCATAGCT 
     4201 GTTTCCTGAA CAGATCCCGG GTGGCATCCC TGTGACCCCT CCCCAGTGCC TCTCCTGGCC 
     4261 CTGGAAGTTG CCACTCCAGT GCCCACCAGC CTTGTCCTAA TAAAATTAAG TTGCATCATT 
     4321 TTGTCTGACT AGGTGTCCTT CTATAATATT ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG 
     4381 GGGCAAGTTG GGAAGACAAC CTGTAGGGCC TGCGGGGTCT ATTGGGAACC AAGCTGGAGT 
     4441 GCAGTGGCAC AATCTTGGCT CACTGCAATC TCCGCCTCCT GGGTTCAAGC GATTCTCCTG 
     4501 CCTCAGCCTC CCGAGTTGTT GGGATTCCAG GCATGCATGA CCAGGCTCAG CTAATTTTTG 
     4561 TTTTTTTGGT AGAGACGGGG TTTCACCATA TTGGCCAGGC TGGTCTCCAA CTCCTAATCT 
     4621 CAGGTGATCT ACCCACCTTG GCCTCCCAAA TTGCTGGGAT TACAGGCGTG AACCACTGCT 
     4681 CCCTTCCCTG TCCTTCTGAT TTTAAAATAA CTATACCAGC AGGAGGACGT CCAGACACAG 
     4741 CATAGGCTAC CTGGCCATGC CCAACCGGTG GGACATTTGA GTTGCTTGCT TGGCACTGTC 
     4801 CTCTCATGCG TTGGGTCCAC TCAGTAGATG CCTGTTGAAT TGGGTACGCG GCCAGCGGCG 
     4861 AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC 
     4921 AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT 
     4981 GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG 
     5041 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC 
     5101 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC 
     5161 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT 
     5221 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT 
     5281 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC 
     5341 AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA 
     5401 GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA 
     5461 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG 
     5521 TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA 
     5581 AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG 
     5641 GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG 
     5701 AAGTTTTAAA TCAATCTAAA GTATATATGA GTAACCTGAG GCTATGGCAG GGCCTGCCGC 
     5761 CCCGACGTTG GCTGCGAGCC CTGGGCCTTC ACCCGAACTT GGGGGGTGGG GTGGGGAAAA 
     5821 GGAAGAAACG CGGGCGTATT GGCCCCAATG GGGTCTCGGT GGGGTATCGA CAGAGTGCCA 
     5881 GCCCTGGGAC CGAACCCCGC GTTTATGAAC AAACGACCCA ACACCGTGCG TTTTATTCTG 
     5941 TCTTTTTATT GCCGTCATAG CGCGGGTTCC TTCCGGTATT GTCTCCTTCC GTGTTTCAGT 
     6001 TAGCCTCCCC CTAGGGTGGG CGAAGAACTC CAGCATGAGA TCCCCGCGCT GGAGGATCAT 
     6061 CCAGCCGGCG TCCCGGAAAA CGATTCCGAA GCCCAACCTT TCATAGAAGG CGGCGGTGGA 
     6121 ATCGAAATCT CGTGATGGCA GGTTGGGCGT CGCTTGGTCG GTCATTTCGA ACCCCAGAGT 
     6181 CCCGCTCAGA AGAACTCGTC AAGAAGGCGA TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG 
     6241 GCGATACCGT AAAGCACGAG GAAGCGGTCA GCCCATTCGC CGCCAAGCTC TTCAGCAATA 
     6301 TCACGGGTAG CCAACGCTAT GTCCTGATAG CGATCCGCCA CACCCAGCCG GCCACAGTCG 
     6361 ATGAATCCAG AAAAGCGGCC ATTTTCCACC ATGATATTCG GCAAGCAGGC ATCGCCATGG 
     6421 GTCACGACGA GATCCTCGCC GTCGGGCATG CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT 
     6481 GGCGCGAGCC CCTGATGCTC TTCGTCCAGA TCATCCTGAT CGACAAGACC GGCTTCCATC 
     6541 CGAGTACGTG CTCGCTCGAT GCGATGTTTC GCTTGGTGGT CGAATGGGCA GGTAGCCGGA 
     6601 TCAAGCGTAT GCAGCCGCCG CATTGCATCA GCCATGATGG ATACTTTCTC GGCAGGAGCA 
     6661 AGGTGAGATG ACAGGAGATC CTGCCCCGGC ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC 
     6721 GCTTCAGTGA CAACGTCGAG CACAGCTGCG CAAGGAACGC CCGTCGTGGC CAGCCACGAT 
     6781 AGCCGCGCTG CCTCGTCTTG CAGTTCATTC AGGGCACCGG ACAGGTCGGT CTTGACAAAA 
     6841 AGAACCGGGC GCCCCTGCGC TGACAGCCGG AACACGGCGG CATCAGAGCA GCCGATTGTC 
     6901 TGTTGTGCCC AGTCATAGCC GAATAGCCTC TCCACCCAAG CGGCCGGAGA ACCTGCGTGC 
     6961 AATCCATCTT GTTCAATCAT GCGAAACGAT CCTCATCCTG TCTCTTGATC GATCTTTGCA 
     7021 AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA CTACTTCTGG AATAGCTCAG AGGCCGAGGC 
     7081 GGCCTCGGCC TCTGCATAAA TAAAAAAAAT TAGTCAGCCA TGGGGCGGAG AATGGGCGGA 
     7141 ACTGGGCGGA GTTAGGGGCG GGATGGGCGG AGTTAGGGGC GGGACTATGG TTGCTGACTA 
     7201 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCT 
     7261 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     7321 CTTTCCACAC CCTAACTGAC ACACATTCCA CAGCTGGTTC TTTCCGCCTC AGGACTCTTC 
     7381 CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT 
     7441 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA 
     7501 CCTGACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG 
     7561 ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC 
     7621 GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GGCTCCCTTT AGGGTTCCGA 
     7681 TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT AGGGTGATGG TTCACGTAGT 
     7741 GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC GTTCTTTAAT 
     7801 AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGTCTA TTCTTTTGAT 
     7861 TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA 
     7921 TTTAACGCGA ATTTT
//