LOCUS       MR211455                7932 bp    DNA     circular  SYN 
DEFINITION  Emilin1 (Myc-DDK-tagged) - Mouse elastin microfibril interfacer 1 (Emilin1)
ACCESSION   NM_133918
VERSION     NM_133918.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4089..4180 
                     /label="Myc-DDK"  
     misc_feature    4210..4795 
                     /label="PolyA signal"  
     misc_feature    4267..4286 
                     /label="Restriction Enzyme"  
     misc_feature    5071..5743 
                     /label="ColE1"  
     misc_feature    6173..6967 
                     /label="Kan/Neo"  
     misc_feature    7002..7304 
                     /label="SV40 ori"  
     misc_feature    7390..7867 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4071..4077 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4071 
                     /label="Emilin1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCCCCCAGA GCCCTCTGGA GCTGCTATCT CTGCTGTCTG CTGACCATAG 
     1081 CCACGGAAGC TGCCAGCTAC CCTCCTCGAG GTTACAGCCT CTACACAGGA GGCACTGGGG 
     1141 CCCTGAGTCC TGGGGGACCC CAGGCCCAGA ACTCACCACG GCCTGCCAGC CGCCACAGGA 
     1201 ACTGGTGTGC CTACGTGGTG ACTCGGACAG TGAGCTGCGT CCTTGAGGAT GGAGTGGAGA 
     1261 CCATTGTCAA GCCAGACTAT CAACCCTGTG GCTGGGGCCA GCCCCACTGT TCCCGAAGTA 
     1321 TCATGTACCG GAGCTTTCTT CGCCCTCGCT ATCGGGTGGC TTACAAGACA GTGACAGACA 
     1381 TGGAGTGGAG GTGCTGTCAG GGTTATGGGG GCGATGACTG TGGAGAGGGC CCTGCTTCCG 
     1441 TTCTGGGCCC AGCACCCTCC ACACCACTTC CCCGGCCCAG GCCTGTGCGC CCCAACCTGT 
     1501 CTGGCTCCAG TGCCGGCAGC CATCTCAGTG GACTGGGAGG AGAAGGTCCT GTTGAGTCAG 
     1561 AGAAGGTGCA GCAGCTAGAG CGGCAGGTGA AGAGTCTGAC CAAAGAGCTG CAAGGCCTTC 
     1621 GGGGTGTCCT GCAGGGGATG AATGGGCGCC TAGCAGAAGA TGTACAGCGA GCTGTGGACA 
     1681 CAGTCTTTAA TGGAAGGCAG CAGCCAGCAG ATGCAGCTGC CCGCCCGGGC GTGCATGAAA 
     1741 CCCTCAGTGA GATCCAGCAG CAGCTGCAGC TCCTGGACAA CCGTGTCTCC ACTCATGACC 
     1801 AGGAGCTGGG CCACCTTAAC AACCATCATA ATGGAGGCCC TGGTGGAGGT GGCAGGGCCT 
     1861 CAGGCCCTGT CCCAGTTCCT TCTGGCCCCA GTGAAGAACT GTTAAGGCAG CTGGAACGGC 
     1921 AGCTGCAAGA GTCTTGCTCA GTGTGCCTGA CGGGGCTGGA TGGCTTCCGC CAGCAGCAGC 
     1981 AAGAGGATAG GGAGCGGCTG CGAACCCTGG AGAAGCTAAT GTCCTCTATG GAGGAGCGGC 
     2041 AGCAACAGCT TGTGGGACCT GCCATGGCCA GGAGACCCCC TCAGGAATGC TGCCCCCCAG 
     2101 AGCTGGGTCG ACGAGTGTCT GAGCTGGAGC GGAGGCTAGA TGTAGTGACT GGCTCACTGA 
     2161 CAGTGCTAAG TGGACGCAGA GGTTCTGAGC TTGGAGGAGC AGCTGGGCAG GGGGGCCACC 
     2221 CTCCAGGCTA CACCAGCTTG GCCTCCCGCC TTTCTCGCCT GGAGGACCGC TTCAACTCTA 
     2281 CCCTAGGTCC CTCAGAGGAG CAAGAGAAGA ACTGGCCTGG AGGACCAGGG AGGCTGGGCC 
     2341 ACTGGTTGCC TGCTGCTCCA GGACGGCTAG AAAAGCTGGA GGGACTACTA GCCAATGTGA 
     2401 GCAGGGAGCT GGGTGGCCGC ATGGATCTGC TGGAAGAGCA GGTGGCAGGG GCTGTTCGGA 
     2461 CTTGTGGGCA GATTTGCTCT GGGGCACCCG GGGAACAGGA TTCTCGGGTC AATGAGATCC 
     2521 TCAGTGCCTT GGAACGCAGG GTGCTGGACA GCGAGGGCCG GTTACAGCTA GTGGGCTCTG 
     2581 GCTTGCATGA AGCAGAGGCA GCAGGGGAGG CTCAGCAGGC CGTGTTGGAG GGACTGCAAG 
     2641 GGCTCCTGAG CCGGCTTCGG GAGCGCATGG ATGCACAGGA GGAGACTGCA GCAGAAATCT 
     2701 TACTGCGCCT CAATCTTACC GCAGCCCAGC TAAGCCAGCT GGAGGGTCTG CTGCAAGCCC 
     2761 GTGGGGATGA GGGTTGTGGC GCCTGTGGTG GTGTCCAGGA GGAGCTGGGC CGCCTTCGGG 
     2821 ATGGTGTGGA ACGTTGCTCC TGCCCATTGT TACCTCCACG GGGCCCTGGA GCTGGCCCAG 
     2881 GGGTTGGGGG ACCAAGCCGT GGGCCTCTGG ATGGTTTCAG TGTGTTTGGG GGCAGTTCAG 
     2941 GCTCAGCCCT CCAGGCCCTT CAAGGAGAAC TCTCTGAGGT TATTCTCACC TTCAGCTCCC 
     3001 TGAACGACTC ACTCCACGAG CTCCAGACCA CTGTGGAGGG TCAGGGTGCC GATCTGGCTG 
     3061 ACCTGGGGGC CACCAAGGAC AGCATCATCT CTGAAATCAA CAGACTACAG CAGGAGGCCA 
     3121 CGGAGCACGT CACAGAGAGC GAGGAGCGCT TCCGAGGCCT GGAGGAGGGC CAGGCACAGG 
     3181 CTGGCCAATG CCCTAGCTTA GAGGGGCGAT TAGGCCGCCT TGAGGGAGTC TGCGAACGAC 
     3241 TGGACACCGT GGCAGGGGGA CTACAAGGCC TACGTGAAGG CCTCTCCAGA CACGTGGCTG 
     3301 GGCTCTGGGC TGCAGTACGG GAAAGCAACA GCACCAGCCT GACACAGGCC GCCCTGCTGG 
     3361 AGAAGCTGCT GGGGGGCCAG GCAGGCCTGG GCAGGCGGCT TGGTGCCCTC AACAATTCCC 
     3421 TGCTGCTCCT GGAAGACCGT CTGCAGCAAC TTAGCCTAAA GGACTTCACT GGACCTTCAG 
     3481 GCAAGGCTGG GCCCCCAGGG CCTCCCGGGC TACAAGGGCC TTCAGGCCCT GCAGGACCTC 
     3541 CAGGACCTCC TGGCAAAGAC GGACAACAGG GGGCCATTGG CCCACCAGGT CCTCAAGGGG 
     3601 AGCAGGGAGC GGAGGGAGCA CCAGCAGCCC CTGTGCCTAG GGTAGCATTT TCAGCTGCCC 
     3661 TGAGTTTGCC ACGGTCAGAA CCTGGCACAG TCCCCTTCGA CAGAGTCTTG CTCAATGATG 
     3721 GAGGCTACTA TGACCCAGAG ACAGGTGTAT TCACTGCACC ACTGGCTGGA CGCTATTTGC 
     3781 TGAGCGCTGT ACTTACTGGG CACCGGCATG AGAAAGTGGA AGCAGTATTG TCACGCTCCA 
     3841 ACCTGGGCGT GGCCCGCATA GACTCGGGAG GCTATGAACC CGAGGGACTG GAGAATAAGC 
     3901 CTGTGGCCGA AAGTCAGCCC AGCCCAGGCG CTCTGGGCGT CTTCAGCCTC ATTCTGCCAC 
     3961 TGCAGGTCGG AGACACTGTC TGCATCGACC TGGTCATGGG GCAGCTGGCA CACTCCGAGG 
     4021 AGCCGCTCAC CATCTTCAGC GGAGCCCTGC TCTACGAGGA CACAGAGCTT GAACAGGTGA 
     4081 CGCGTACGCG GCCGCTCGAG CAGAAACTCA TCTCAGAAGA GGATCTGGCA GCAAATGATA 
     4141 TCCTGGATTA CAAGGATGAC GACGATAAGG TTTAAACGGC CGGCCGCGGT CATAGCTGTT 
     4201 TCCTGAACAG ATCCCGGGTG GCATCCCTGT GACCCCTCCC CAGTGCCTCT CCTGGCCCTG 
     4261 GAAGTTGCCA CTCCAGTGCC CACCAGCCTT GTCCTAATAA AATTAAGTTG CATCATTTTG 
     4321 TCTGACTAGG TGTCCTTCTA TAATATTATG GGGTGGAGGG GGGTGGTATG GAGCAAGGGG 
     4381 CAAGTTGGGA AGACAACCTG TAGGGCCTGC GGGGTCTATT GGGAACCAAG CTGGAGTGCA 
     4441 GTGGCACAAT CTTGGCTCAC TGCAATCTCC GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT 
     4501 CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA TGCATGACCA GGCTCAGCTA ATTTTTGTTT 
     4561 TTTTGGTAGA GACGGGGTTT CACCATATTG GCCAGGCTGG TCTCCAACTC CTAATCTCAG 
     4621 GTGATCTACC CACCTTGGCC TCCCAAATTG CTGGGATTAC AGGCGTGAAC CACTGCTCCC 
     4681 TTCCCTGTCC TTCTGATTTT AAAATAACTA TACCAGCAGG AGGACGTCCA GACACAGCAT 
     4741 AGGCTACCTG GCCATGCCCA ACCGGTGGGA CATTTGAGTT GCTTGCTTGG CACTGTCCTC 
     4801 TCATGCGTTG GGTCCACTCA GTAGATGCCT GTTGAATTGG GTACGCGGCC AGCGGCGAGC 
     4861 GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG 
     4921 AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT 
     4981 GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA 
     5041 GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT 
     5101 CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC 
     5161 GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT 
     5221 TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC 
     5281 CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC 
     5341 CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG 
     5401 GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC 
     5461 AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG 
     5521 CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA 
     5581 TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT 
     5641 TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG 
     5701 TTTTAAATCA ATCTAAAGTA TATATGAGTA ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC 
     5761 GACGTTGGCT GCGAGCCCTG GGCCTTCACC CGAACTTGGG GGGTGGGGTG GGGAAAAGGA 
     5821 AGAAACGCGG GCGTATTGGC CCCAATGGGG TCTCGGTGGG GTATCGACAG AGTGCCAGCC 
     5881 CTGGGACCGA ACCCCGCGTT TATGAACAAA CGACCCAACA CCGTGCGTTT TATTCTGTCT 
     5941 TTTTATTGCC GTCATAGCGC GGGTTCCTTC CGGTATTGTC TCCTTCCGTG TTTCAGTTAG 
     6001 CCTCCCCCTA GGGTGGGCGA AGAACTCCAG CATGAGATCC CCGCGCTGGA GGATCATCCA 
     6061 GCCGGCGTCC CGGAAAACGA TTCCGAAGCC CAACCTTTCA TAGAAGGCGG CGGTGGAATC 
     6121 GAAATCTCGT GATGGCAGGT TGGGCGTCGC TTGGTCGGTC ATTTCGAACC CCAGAGTCCC 
     6181 GCTCAGAAGA ACTCGTCAAG AAGGCGATAG AAGGCGATGC GCTGCGAATC GGGAGCGGCG 
     6241 ATACCGTAAA GCACGAGGAA GCGGTCAGCC CATTCGCCGC CAAGCTCTTC AGCAATATCA 
     6301 CGGGTAGCCA ACGCTATGTC CTGATAGCGA TCCGCCACAC CCAGCCGGCC ACAGTCGATG 
     6361 AATCCAGAAA AGCGGCCATT TTCCACCATG ATATTCGGCA AGCAGGCATC GCCATGGGTC 
     6421 ACGACGAGAT CCTCGCCGTC GGGCATGCTC GCCTTGAGCC TGGCGAACAG TTCGGCTGGC 
     6481 GCGAGCCCCT GATGCTCTTC GTCCAGATCA TCCTGATCGA CAAGACCGGC TTCCATCCGA 
     6541 GTACGTGCTC GCTCGATGCG ATGTTTCGCT TGGTGGTCGA ATGGGCAGGT AGCCGGATCA 
     6601 AGCGTATGCA GCCGCCGCAT TGCATCAGCC ATGATGGATA CTTTCTCGGC AGGAGCAAGG 
     6661 TGAGATGACA GGAGATCCTG CCCCGGCACT TCGCCCAATA GCAGCCAGTC CCTTCCCGCT 
     6721 TCAGTGACAA CGTCGAGCAC AGCTGCGCAA GGAACGCCCG TCGTGGCCAG CCACGATAGC 
     6781 CGCGCTGCCT CGTCTTGCAG TTCATTCAGG GCACCGGACA GGTCGGTCTT GACAAAAAGA 
     6841 ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC ACGGCGGCAT CAGAGCAGCC GATTGTCTGT 
     6901 TGTGCCCAGT CATAGCCGAA TAGCCTCTCC ACCCAAGCGG CCGGAGAACC TGCGTGCAAT 
     6961 CCATCTTGTT CAATCATGCG AAACGATCCT CATCCTGTCT CTTGATCGAT CTTTGCAAAA 
     7021 GCCTAGGCCT CCAAAAAAGC CTCCTCACTA CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC 
     7081 CTCGGCCTCT GCATAAATAA AAAAAATTAG TCAGCCATGG GGCGGAGAAT GGGCGGAACT 
     7141 GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT TAGGGGCGGG ACTATGGTTG CTGACTAATT 
     7201 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCTGGT 
     7261 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     7321 TCCACACCCT AACTGACACA CATTCCACAG CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT 
     7381 TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA 
     7441 TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT 
     7501 GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC 
     7561 GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC 
     7621 ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT 
     7681 AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG 
     7741 CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT 
     7801 GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA 
     7861 TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT 
     7921 AACGCGAATT TT
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