LOCUS       MR211447                7920 bp    DNA     circular  SYN 
DEFINITION  Tcof1 (Myc-DDK-tagged) - Mouse Treacher Collins Franceschetti syndrome 1, homolog (cDNA clone MGC:36035 IMAGE:5351783)
ACCESSION   BC021514
VERSION     BC021514.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4077..4168 
                     /label="Myc-DDK"  
     misc_feature    4198..4783 
                     /label="PolyA signal"  
     misc_feature    4255..4274 
                     /label="Restriction Enzyme"  
     misc_feature    5059..5731 
                     /label="ColE1"  
     misc_feature    6161..6955 
                     /label="Kan/Neo"  
     misc_feature    6990..7292 
                     /label="SV40 ori"  
     misc_feature    7378..7855 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4059..4065 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4059 
                     /label="Tcof1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCCGAGGCC AGGAAGCGGC GGGAGCTGCT TCCCCTCATT TACCATCATC 
     1081 TGTTGCAAGC AGGCTACGTC CGCGCGGCGC GGGAAGTAAA GGAGCAGAGC GGCCAGAAGA 
     1141 GTTTCCTGAC TCAGCCCGTC ACCCTTCTGG ACATCTATAC ACACTGGCAA CAGACCTCAG 
     1201 AGCTTGGCCA GAAGCAGAAG GCAGAGGATG ATGAGACCCT TCAGGCTAAG AAGTCTCGAG 
     1261 TGTCGGATCC TGTTAGCAGC TCAGAGAGCT CGGATCAGGA GAAGGAAGAG GAGGCAGCAA 
     1321 CCGAAAGGGC CAAAGCCACC CCAAGACCGA CACCTGTCAA TTCTGCAACC GCAGCTTTGC 
     1381 CATCAAAAGT AAAAGAAAAG GGAAAGACCA AGACAGCCAA CAAGACGGTG AACTCTGTGT 
     1441 CGCACCCTGG GTCCGGAAAG ACGGTGGTCC ACCTGCTCTC TGGGAAGTCA CCCAAGAAGT 
     1501 CAGCAGAGCC CTTGGCAAAC ACTGTCTTGG CCTCAGAAAC TGAGGAGGAG GGCAATGCCC 
     1561 AAGCCCTCGG ACCCACTGCC AAGTCTGGAA CGGTGTCAGC GGGCCAAGGC AGCAGTTCCA 
     1621 GTGAAGATTC CTCCATCTCA AGCGATGAGA CAGATGTCGA GGTGAAATCT CCAGCAAAAC 
     1681 CAGCCCAGGC CAAAGCTTCA GCAGCCCCTG CCAAGGATCC TCCAGCAAGA ACAGCCCCAG 
     1741 GCCCTACCAA GTTAGGGAAT GTGGCGCCCA CACCTGCTAA ACCAGCCAGG GCGGCAGCGG 
     1801 CAGCTGCTGC TGCTGCTGTG GCTGCTGCTG CTGCTGCAGC AGCAGAAGAG TCTGAGAGCA 
     1861 GTGAGGAGGA CTCAGACAGT GAGGACGAGG CCCCTGCTGG TCTGCCCAGC CAGGTAAAAG 
     1921 CCTCTGGAAA AGGTCCCCAT GTCAGAGCCG ACTCGGTGTC TGCCAAGGGG ATCTCTGGGA 
     1981 AAGGGCCCAT CTTAGCAACC CCAGGGAAGA CTGGGCCTGC AGCCACCCAG GCCAAGGCAG 
     2041 AAAGGCCAGA GAAGGACTCG GAAACCAGCA GTGAGGACGA TTCTGATAGT GAGGATGAAA 
     2101 TGCCAGTCAC TGTGAATACT CCTCAGGCAA GGACTTCTGG GAAGAGCCCT CGGGCCAGAG 
     2161 GTACCTCAGC CCCCGCCAAG GAGTCATCCC AGAAAGGGGC TCCTGCAGTC ACCCCTGGAA 
     2221 AGGCAAGGCC TGTGGCAGCC CAGGCAGGGA AACCAGAAGC CAAGAGCAGT GAGGAGTCAG 
     2281 AGAGTGACAG TGGGGAGACA CCAGCTGCTG CGACTCTGAC CACGAGTCCT GCCAAGGTGA 
     2341 AACCTTTGGG GAAGAGCTCC CAGGTCAGAC CTGTTTCCAC CGTCACCCCG GGGTCATCGG 
     2401 GAAAAGGTGC CAACCTGCCC TGCCCTGGGA AGGTGGGGTC AGCAGCTCTC AGGGTCCAAA 
     2461 TGGTAAAGAA AGAAGATGTC TCGGAGAGCA GCAGTGCAGA GCTGGACAGT GACGGGCCTG 
     2521 GGAGCCCAGC CAAGGCAAAG GCCTCCCTTG CGCTCCCTCA GAAGGTGAGG CCTGTGGCCA 
     2581 CCCAGGTCAA GACTGACAGG GGCAAAGGCC ACTCAGGGAG CAGTGAGGAG TCATCTGACA 
     2641 GCGAAGAGGA GGCAGCACCA GCAGCCTCTG CTGCTCAGGC TAAGCCAGCT CTGGAAAAGC 
     2701 AGATGAAAGC TTCCTCTAGG AAAGGCACGC CTGCATCCGC AACAGGAGCG AGCACCTCGT 
     2761 CCCATTGTAA GGCAGGAGCG GTGACCTCTT CAGCCAGCCT GTCATCCCCA GCTCTGGCCA 
     2821 AGGGCACCCA GAGGTCAGAT GTGGACTCTT CCAGTGAGTC TGAGTCAGAA GGAGCTGCTC 
     2881 CCAGCACCCC CAGGGTACAG GGGAAGTCTG GGGGCAAGGG CCTCCAAGGG AAAGCTGCCT 
     2941 TGGGGCAAGG GGTGGCCCCA GTGCACACTC AGAAGACAGG GCCTTCGGTC AAAGCTATGG 
     3001 CTCAGGAAGA CTCAGAGAGC CTCGAGGAGG ACTCCAGCAG CGAGGAAGAG GATGAGACCC 
     3061 CAGCACAGGC CACGCCCTTG GGGAGACTTC CTCAGGCCAA AGCCAACCCA CCTCCCACTA 
     3121 AGACACCTCC AGCGTCTGCA TCTGGAAAAG CTGTGGCTGC TCCAACCAAG GGAAAACCAC 
     3181 CTGTTCCGAA CAGCACCGTC TCTGCAAGGG GCCAGCGGTC TGTGCCAGCC GCGGGAAAAG 
     3241 CAGGGGCCCC AGCAACCCAA GCCCAGAAGG GTCCCGTGGC TGGCACAGGG GAGGACTCAG 
     3301 AGAGCAGCAG TAAAGAGGAG TCTGACAGTG AAGAAGAGAC GCCAGCCCAG ATAAAACCTG 
     3361 TGGGGAAGAC CTCTCAGGTC AGAGCTGCCT CAGCCCCTGC CAAGGAGTCT CCTAAAAAAG 
     3421 GAGCCCATCC AGGAACCCCC GGCAAGACGG GATCTTCAGC TACCCAGGCC CAGCCAGGGA 
     3481 AGACAGAGGA CTCAGACAGC AGTAGTGAAG AGTCTGACAG TGACACAGAG ATGCCATCAG 
     3541 CCCAGGATGC TATCTCCCAG CCTGCCAGAG GCAAAGCGTC AGGTCCAGCG TCCCCAGAGA 
     3601 AGAGCATAGA AGGGTCCTCA GAGAGCAGTG ATGAGGATCT GCCCTCCGGC CAGGCGATTA 
     3661 AATCCCCTCC AGTTTCTGTC AACCGTAACA GTAGTCCAGC TGTCCCAGCT CCTACCCCAG 
     3721 AAGGAGTCCA GGCTGTGAAC ACCACAAAGA AGGCCTCAGG CACCACTGCC CAGAGCTCCT 
     3781 CCTCTGAGAG TGAGGACGGG GACGAGGACT TGATTCCTGC CACACAACCC TCCACCTATG 
     3841 CTCTCAGAAC CAGTGTGACG ACGCCCGCAG CCCTCTCACG AGCAGCTTCC CAACCCAGCA 
     3901 AGAGTGAGCA GTCTAGCCGG ATGCCAAAAG GCAAGAAAGC AAAGGCGGCG GCGTCCGCTC 
     3961 AGGTACTGTC GGACCAGCAG TGCCGTGGAA ACACTCCCCA TGATGCCTCC CCAGAGCGCA 
     4021 CCCATCCAGC CCAAAGCCAC CAACAAGCTC GGGAAATCCA AGCTCCCACG CGTACGCGGC 
     4081 CGCTCGAGCA GAAACTCATC TCAGAAGAGG ATCTGGCAGC AAATGATATC CTGGATTACA 
     4141 AGGATGACGA CGATAAGGTT TAAACGGCCG GCCGCGGTCA TAGCTGTTTC CTGAACAGAT 
     4201 CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA GTGCCTCTCC TGGCCCTGGA AGTTGCCACT 
     4261 CCAGTGCCCA CCAGCCTTGT CCTAATAAAA TTAAGTTGCA TCATTTTGTC TGACTAGGTG 
     4321 TCCTTCTATA ATATTATGGG GTGGAGGGGG GTGGTATGGA GCAAGGGGCA AGTTGGGAAG 
     4381 ACAACCTGTA GGGCCTGCGG GGTCTATTGG GAACCAAGCT GGAGTGCAGT GGCACAATCT 
     4441 TGGCTCACTG CAATCTCCGC CTCCTGGGTT CAAGCGATTC TCCTGCCTCA GCCTCCCGAG 
     4501 TTGTTGGGAT TCCAGGCATG CATGACCAGG CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA 
     4561 CGGGGTTTCA CCATATTGGC CAGGCTGGTC TCCAACTCCT AATCTCAGGT GATCTACCCA 
     4621 CCTTGGCCTC CCAAATTGCT GGGATTACAG GCGTGAACCA CTGCTCCCTT CCCTGTCCTT 
     4681 CTGATTTTAA AATAACTATA CCAGCAGGAG GACGTCCAGA CACAGCATAG GCTACCTGGC 
     4741 CATGCCCAAC CGGTGGGACA TTTGAGTTGC TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG 
     4801 TCCACTCAGT AGATGCCTGT TGAATTGGGT ACGCGGCCAG CGGCGAGCGG TATCAGCTCA 
     4861 CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG 
     4921 AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA 
     4981 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA 
     5041 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC 
     5101 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC 
     5161 GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT 
     5221 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG 
     5281 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG 
     5341 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA 
     5401 CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG 
     5461 AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT 
     5521 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT 
     5581 TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG 
     5641 ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT 
     5701 CTAAAGTATA TATGAGTAAC CTGAGGCTAT GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC 
     5761 GAGCCCTGGG CCTTCACCCG AACTTGGGGG GTGGGGTGGG GAAAAGGAAG AAACGCGGGC 
     5821 GTATTGGCCC CAATGGGGTC TCGGTGGGGT ATCGACAGAG TGCCAGCCCT GGGACCGAAC 
     5881 CCCGCGTTTA TGAACAAACG ACCCAACACC GTGCGTTTTA TTCTGTCTTT TTATTGCCGT 
     5941 CATAGCGCGG GTTCCTTCCG GTATTGTCTC CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG 
     6001 GTGGGCGAAG AACTCCAGCA TGAGATCCCC GCGCTGGAGG ATCATCCAGC CGGCGTCCCG 
     6061 GAAAACGATT CCGAAGCCCA ACCTTTCATA GAAGGCGGCG GTGGAATCGA AATCTCGTGA 
     6121 TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT TTCGAACCCC AGAGTCCCGC TCAGAAGAAC 
     6181 TCGTCAAGAA GGCGATAGAA GGCGATGCGC TGCGAATCGG GAGCGGCGAT ACCGTAAAGC 
     6241 ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA AGCTCTTCAG CAATATCACG GGTAGCCAAC 
     6301 GCTATGTCCT GATAGCGATC CGCCACACCC AGCCGGCCAC AGTCGATGAA TCCAGAAAAG 
     6361 CGGCCATTTT CCACCATGAT ATTCGGCAAG CAGGCATCGC CATGGGTCAC GACGAGATCC 
     6421 TCGCCGTCGG GCATGCTCGC CTTGAGCCTG GCGAACAGTT CGGCTGGCGC GAGCCCCTGA 
     6481 TGCTCTTCGT CCAGATCATC CTGATCGACA AGACCGGCTT CCATCCGAGT ACGTGCTCGC 
     6541 TCGATGCGAT GTTTCGCTTG GTGGTCGAAT GGGCAGGTAG CCGGATCAAG CGTATGCAGC 
     6601 CGCCGCATTG CATCAGCCAT GATGGATACT TTCTCGGCAG GAGCAAGGTG AGATGACAGG 
     6661 AGATCCTGCC CCGGCACTTC GCCCAATAGC AGCCAGTCCC TTCCCGCTTC AGTGACAACG 
     6721 TCGAGCACAG CTGCGCAAGG AACGCCCGTC GTGGCCAGCC ACGATAGCCG CGCTGCCTCG 
     6781 TCTTGCAGTT CATTCAGGGC ACCGGACAGG TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC 
     6841 TGCGCTGACA GCCGGAACAC GGCGGCATCA GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA 
     6901 TAGCCGAATA GCCTCTCCAC CCAAGCGGCC GGAGAACCTG CGTGCAATCC ATCTTGTTCA 
     6961 ATCATGCGAA ACGATCCTCA TCCTGTCTCT TGATCGATCT TTGCAAAAGC CTAGGCCTCC 
     7021 AAAAAAGCCT CCTCACTACT TCTGGAATAG CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC 
     7081 ATAAATAAAA AAAATTAGTC AGCCATGGGG CGGAGAATGG GCGGAACTGG GCGGAGTTAG 
     7141 GGGCGGGATG GGCGGAGTTA GGGGCGGGAC TATGGTTGCT GACTAATTGA GATGCATGCT 
     7201 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCTGGTTG CTGACTAATT 
     7261 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCCTAA 
     7321 CTGACACACA TTCCACAGCT GGTTCTTTCC GCCTCAGGAC TCTTCCTTTT TCAATATTAT 
     7381 TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA 
     7441 AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA CGCGCCCTGT 
     7501 AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC 
     7561 AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC 
     7621 TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG 
     7681 CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA 
     7741 TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC 
     7801 CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT TTGATTTATA AGGGATTTTG 
     7861 CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//