LOCUS       MR211437                7914 bp    DNA     circular  SYN 
DEFINITION  Tnk2 (Myc-DDK-tagged) - Mouse tyrosine kinase, non-receptor, 2 (Tnk2), transcript variant 2
ACCESSION   NM_001110147
VERSION     NM_001110147.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4069..4160 
                     /label="Myc-DDK"  
     misc_feature    4190..4775 
                     /label="PolyA signal"  
     misc_feature    4247..4266 
                     /label="Restriction Enzyme"  
     misc_feature    5051..5723 
                     /label="ColE1"  
     misc_feature    6153..6947 
                     /label="Kan/Neo"  
     misc_feature    6982..7284 
                     /label="SV40 ori"  
     misc_feature    7370..7847 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4044..4051 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4044 
                     /label="Tnk2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCAGCCGGAG GAGGGAACGG GCTGGCTGTT GGAGCTGCTG TCCGAGGTGC 
     1081 AGCTACAACA GTATTTCCTG AGGCTTCGAG ATGACCTCAA CATTACCCGC CTATCTCATT 
     1141 TTGAATATGT CAAAAATGAA GACCTGGAAA AGATTGGCAT GGGCCGGCCT GGCCAGAGGC 
     1201 GGCTGTGGGA GGCCGTGAAG AGGAGGAAGG CCATGTGCAA ACGCAAGTCA TGGATGAGCA 
     1261 AGGTGTTCAG TGGAAAGCGG TTGGAGGCCG AGTTCCCTTC CCAGCACTCT CAGAGCACCT 
     1321 TCCGGAAGCC CTCCCCCACC CCAGGGAGCC TGCCAGGGGA GGGGACCCTG CAGAGCCTCA 
     1381 CCTGCCTCAT TGGGGAGAAA GACCTACGCC TGCTGGAGAA GCTGGGGGAT GGCTCCTTTG 
     1441 GCGTGGTGCG CAGGGGTGAA TGGGACGCCC CCGCAGGGAA GACGGTGAGT GTGGCCGTCA 
     1501 AGTGCCTGAA GCCTGACGTG CTGAGCCAGC CAGAGGCCAT GGACGACTTC ATCCGGGAGG 
     1561 TCAATGCCAT GCATTCACTA GACCACCGAA ACCTCATTCG CTTGTATGGT GTGGTGCTCA 
     1621 CACTACCCAT GAAGATGGTG ACAGAGCTGG CACCTCTGGG ATCTTTGTTG GACCGCCTAC 
     1681 GTAAACACCA AGGTCATTTC CTCTTGGGGA CGCTGAGTCG CTACGCTGTG CAGGTGGCTG 
     1741 AGGGTATGGC CTACCTGGAG TCCAAGCGCT TCATTCACCG GGATCTGGCT GCTCGAAACC 
     1801 TGCTTTTGGC TACCCGGGAC CTGGTCAAGA TTGGGGACTT CGGACTGATG CGAGCTCTGC 
     1861 CCCAGAATGA TGACCACTAT GTCATGCAAG AACACCGCAA GGTGCCCTTT GCCTGGTGTG 
     1921 CCCCTGAGAG CCTGAAGACA CGGACTTTCT CCCATGCCAG TGACACCTGG ATGTTTGGGG 
     1981 TCACACTGTG GGAGATGTTC ACCTATGGCC AGGAGCCCTG GATTGGCCTC AATGGCAGCC 
     2041 AGATCCTGCA TAAGATCGAC AAGGAAGGGG AGCGCCTGCC CCGGCCGGAG GACTGCCCTC 
     2101 AAGACATCTA CAATGTCATG GTCCAGTGCT GGGCCCACAA GCCAGAGGAC AGACCCACAT 
     2161 TTGTGGCTCT TCGGGACTTC CTGCTGGAGG CTCAGCCCAC TGACATGCGG GCTCTTCAGG 
     2221 ACTTTGAGGA GCCAGATAAA CTGCACATCC AGATGAATGA CGTCATCACT GTCATCGAGG 
     2281 GAAGGGCTGA GAACTACTGG TGGCGTGGGC AGAATACGCG GACCCTGTGT GTAGGACCCT 
     2341 TCCCTCGAAA TGTCGTGACC TCCGTGGCTG GCCTGTCAGC CCAGGACATC AGCCAGCCTC 
     2401 TACAGAATAG CTTCATTCAC ACAGGACATG GTGACAGTGA CCCCCGCCAC TGCTGGGGGT 
     2461 TCCCTGACAG GATCGATGAA CTGTACCTGG GAAACCCCAT GGACCCTCCT GACCTGCTGA 
     2521 GTGTGGAACT GAGCACCTCC CGACCCACCC AGCACCTAGG ACGGGTGAAA AAGCCAACAT 
     2581 ACGACCCTGT GAGTGAGGAC CCAGACCCCC TGTCCAGCGA CTTCAAGAGG CTTGGCCTGA 
     2641 GGAAGCCAGC CCTGCCTCGA GGGCTGTGGC TGGCAAAGCC CTCAGCCCGA GTGCCAGGCA 
     2701 CCAAGGCAGA CCGCAGCAGT GGGGGTGAGG TCACACTCAT CGACTTCGGT GAGGAGCCTG 
     2761 TGGTCCCAAC CCCTCGGCCC TGTGCACCCT CCTTGGCACA GTTGGCCATG GATGCCTGCT 
     2821 CCTTGCTGGA CAAGACACCA CCACAGAGCC CCACACGGGC ACTGCCACGA CCTTTACACC 
     2881 CCACCCCTGT GGTAGACTGG GACGCGCGTC CGCTGCCCCC GCCCCCTGCC TATGACGACG 
     2941 TGGCCCAGGA TGAGGATGAC TTTGAGGTCT GCTCCATCAA CAGCACGCTA GTAGGTGCAG 
     3001 GCCTCCCTGC TGGGCCTAGC CAAGGCGAGA CCAATTACGC CTTTGTACCT GAGCAGGCGC 
     3061 AGATGCCCCC TGCCCTGGAG GATAACCTGT TCCTTCCACC CCAGGGCGGA GGCAAGCCGC 
     3121 CCAGCTCAGT GCAGACTGCA GAGATTTTCC AGGCACTGCA GCAGGAGTGT ATGCGGCAGC 
     3181 TACAGGTCCC CACTGGCCAG CTGACCCCCT CCCCGACCCC AGGAGGTGAT GACAAGCCCC 
     3241 AGGTGCCACC CCGGGTACCT ATTCCCCCTC GGCCCACGCG TCCACGTGTG GAGCTATCTC 
     3301 CAGCTCCCTC GGGTGAGGAA GAGACAAGCC GGTGGCCTGG ACCTGCCTCG CCCCCCCGAG 
     3361 TGCCTCCCCG GGAACCTCTG TCTCCTCAAG GTTCAAGGAC CCCAAGCCCC CTAGTGCCAC 
     3421 CTGGCAGCTC TCCACTACCG CATCGGCTCT CTAGCTCACC TGGAAAGACC ATGCCCACCA 
     3481 CTCAAAGCTT TGCCTCAGAC CCTAAGTATG CCACTCCACA AGTGATCCAG GCTCCTGGCC 
     3541 CACGGGCAGG CCCCTGCATC CTGCCCATTG TCCGCGATGG CAGGAAGGTC AGCAGCACTC 
     3601 ATTACTACCT GCTGCCTGAG CGCCCTCCTT ACCTGGAACG CTATCAGCGC TTCCTGCGGG 
     3661 AGGCCCAGAG CCCGGAAGAG CCGGCCGCCC TGCCTGTGCC CCCGCTGTTG CCCCCGCCCA 
     3721 GTACTCCAGC CCCTGCTGCC CCCACTGCCA CCGTCAGACC TATGCCTCAG GCCGCCCCAG 
     3781 ACCCAAAGGC CAACTTCTCC ACCAATAACA GCAACCCAGG GGCACGGCCA CCATCCCTGA 
     3841 GGGCCACGGC TCGGCTGCCA CAGAGGGGCT GCCCAGGGGA CGGGCAAGAG GCTGCTCGGC 
     3901 CAGCAGACAA GGTCCAGATG GTGGAGCAGC TCTTTGGGCT GGGTCTTCGG CCACGGGTGG 
     3961 AGTGCCACAA GGTCCTAGAG ATGTTCGACT GGAACCTAGA GCAAGCCGGC TGTCACCTTC 
     4021 TGGGCTCCTG TGGCCCTGCT CATCACAAAC GCAGCGGACC GACGCGTACG CGGCCGCTCG 
     4081 AGCAGAAACT CATCTCAGAA GAGGATCTGG CAGCAAATGA TATCCTGGAT TACAAGGATG 
     4141 ACGACGATAA GGTTTAAACG GCCGGCCGCG GTCATAGCTG TTTCCTGAAC AGATCCCGGG 
     4201 TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG 
     4261 CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC 
     4321 TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC 
     4381 TGTAGGGCCT GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC 
     4441 ACTGCAATCT CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG 
     4501 GGATTCCAGG CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT 
     4561 TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG 
     4621 CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT 
     4681 TTAAAATAAC TATACCAGCA GGAGGACGTC CAGACACAGC ATAGGCTACC TGGCCATGCC 
     4741 CAACCGGTGG GACATTTGAG TTGCTTGCTT GGCACTGTCC TCTCATGCGT TGGGTCCACT 
     4801 CAGTAGATGC CTGTTGAATT GGGTACGCGG CCAGCGGCGA GCGGTATCAG CTCACTCAAA 
     4861 GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA 
     4921 AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT 
     4981 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 
     5041 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 
     5101 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 
     5161 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 
     5221 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 
     5281 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 
     5341 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 
     5401 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 
     5461 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 
     5521 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC 
     5581 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC 
     5641 AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG 
     5701 TATATATGAG TAACCTGAGG CTATGGCAGG GCCTGCCGCC CCGACGTTGG CTGCGAGCCC 
     5761 TGGGCCTTCA CCCGAACTTG GGGGGTGGGG TGGGGAAAAG GAAGAAACGC GGGCGTATTG 
     5821 GCCCCAATGG GGTCTCGGTG GGGTATCGAC AGAGTGCCAG CCCTGGGACC GAACCCCGCG 
     5881 TTTATGAACA AACGACCCAA CACCGTGCGT TTTATTCTGT CTTTTTATTG CCGTCATAGC 
     5941 GCGGGTTCCT TCCGGTATTG TCTCCTTCCG TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC 
     6001 GAAGAACTCC AGCATGAGAT CCCCGCGCTG GAGGATCATC CAGCCGGCGT CCCGGAAAAC 
     6061 GATTCCGAAG CCCAACCTTT CATAGAAGGC GGCGGTGGAA TCGAAATCTC GTGATGGCAG 
     6121 GTTGGGCGTC GCTTGGTCGG TCATTTCGAA CCCCAGAGTC CCGCTCAGAA GAACTCGTCA 
     6181 AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA TCGGGAGCGG CGATACCGTA AAGCACGAGG 
     6241 AAGCGGTCAG CCCATTCGCC GCCAAGCTCT TCAGCAATAT CACGGGTAGC CAACGCTATG 
     6301 TCCTGATAGC GATCCGCCAC ACCCAGCCGG CCACAGTCGA TGAATCCAGA AAAGCGGCCA 
     6361 TTTTCCACCA TGATATTCGG CAAGCAGGCA TCGCCATGGG TCACGACGAG ATCCTCGCCG 
     6421 TCGGGCATGC TCGCCTTGAG CCTGGCGAAC AGTTCGGCTG GCGCGAGCCC CTGATGCTCT 
     6481 TCGTCCAGAT CATCCTGATC GACAAGACCG GCTTCCATCC GAGTACGTGC TCGCTCGATG 
     6541 CGATGTTTCG CTTGGTGGTC GAATGGGCAG GTAGCCGGAT CAAGCGTATG CAGCCGCCGC 
     6601 ATTGCATCAG CCATGATGGA TACTTTCTCG GCAGGAGCAA GGTGAGATGA CAGGAGATCC 
     6661 TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG TCCCTTCCCG CTTCAGTGAC AACGTCGAGC 
     6721 ACAGCTGCGC AAGGAACGCC CGTCGTGGCC AGCCACGATA GCCGCGCTGC CTCGTCTTGC 
     6781 AGTTCATTCA GGGCACCGGA CAGGTCGGTC TTGACAAAAA GAACCGGGCG CCCCTGCGCT 
     6841 GACAGCCGGA ACACGGCGGC ATCAGAGCAG CCGATTGTCT GTTGTGCCCA GTCATAGCCG 
     6901 AATAGCCTCT CCACCCAAGC GGCCGGAGAA CCTGCGTGCA ATCCATCTTG TTCAATCATG 
     6961 CGAAACGATC CTCATCCTGT CTCTTGATCG ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA 
     7021 GCCTCCTCAC TACTTCTGGA ATAGCTCAGA GGCCGAGGCG GCCTCGGCCT CTGCATAAAT 
     7081 AAAAAAAATT AGTCAGCCAT GGGGCGGAGA ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG 
     7141 GATGGGCGGA GTTAGGGGCG GGACTATGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     7201 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCTG GTTGCTGACT AATTGAGATG 
     7261 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC CTAACTGACA 
     7321 CACATTCCAC AGCTGGTTCT TTCCGCCTCA GGACTCTTCC TTTTTCAATA TTATTGAAGC 
     7381 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA 
     7441 CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC 
     7501 GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC 
     7561 CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC 
     7621 CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC 
     7681 GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG 
     7741 GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT 
     7801 GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT 
     7861 TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
//