LOCUS       MR211427                7890 bp    DNA     circular  SYN 
DEFINITION  Hltf (Myc-DDK-tagged) - Mouse helicase-like transcription factor (Hltf), transcript variant 1
ACCESSION   NM_009210
VERSION     NM_009210.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4047..4138 
                     /label="Myc-DDK"  
     misc_feature    4168..4753 
                     /label="PolyA signal"  
     misc_feature    4225..4244 
                     /label="Restriction Enzyme"  
     misc_feature    5029..5701 
                     /label="ColE1"  
     misc_feature    6131..6925 
                     /label="Kan/Neo"  
     misc_feature    6960..7262 
                     /label="SV40 ori"  
     misc_feature    7348..7825 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4029..4035 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4029 
                     /label="Hltf"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCCTATACG TTCACGAGGG GTCCTGTTTG GAAGTATTCG CAGTCTGTCC 
     1081 AGTATGGATC ACACGAAAAT ATTCCAAGAC TTTCATACTC AACTTTCTTG CCACATTTTG 
     1141 AATTCCAAGA CATTATTCCT CCAGATGACT TCCTAACGAG TGATGAAGAG CAAGATTTGG 
     1201 TTTTATTTGG AACTATGAGA GGACAAGTTG TTGGACTACG CTATTACACA GGAGTTGTAA 
     1261 ATAATAATGA AATGGTTGCT TTACAACGAG AGCCTAATAA TCCCTATGAT AAGAATGCTA 
     1321 TTAAAGTAAA TAATGTGAAT GGAAATCAGG TTGGCCATAT AAAGAGAGAG ATTGCAGCTG 
     1381 CCGTGGCCTA TATCATGGAC AACAAATTGG CACAAGTGGA AGGTGTAGTT CCTTTTGGTG 
     1441 CAAGTAATAC TTTTACCATG CCTCTTTATA TGACTTTCTG GGGAAAAGAA GAAAATAGAA 
     1501 ACGTGGTTTT AGAGCAGTTG AAGAAACATG GATTTAAGTT GGGTCCCACA CCAAAGACTT 
     1561 TAGGGTCGAG TTTGGAGAAC GCATGGGGCT CTGGAAGAGC TGGACCAAGC TATAGTAGGC 
     1621 CGGCTCATGT GGCAGTACAG ATGACAACTG ACCAGCTCAA AACGGAATTT GACAAATTAT 
     1681 TTGAAGATTT AAAAGAAGAT GATAGAACTG TTGAAATGGA ACCAGCTGAG GCTATTGAAA 
     1741 CACCGTTGCT TCCACACCAA AAACAAGCTC TAGCTTGGAT GATCGCACGA GAGAACAGTA 
     1801 AAGAACTACC ACCATTCTGG GAACAGCGCA ATGACTTATA TTATAATACA ATAACAAATT 
     1861 TTTCTGTGAA GGAACGACCG GAAAATGTTC ATGGAGGAAT TTTAGCGGAT GATATGGGTT 
     1921 TGGGCAAAAC TCTTACTGCT ATTGCTGTCA TTCTTACCAA CTTTGATGAT GGCAGGCCTC 
     1981 TTCTCAGTAA GAGAGGCAAG AAGAATCATC CAGGAAAGGA ATACAAGGAT GAGACCATTA 
     2041 AACGCAGAGG AAGTAATATG GACAAGAAGG AAGATGGACA CTCAGAGTCA TCCACGTGTG 
     2101 GTGAAGAACC CAGTATTTCA GGAACTCCGG AGAAGAGTAG CTGTACTCTG TCGCAGCTGT 
     2161 CTAGCGTCTG CCCTAAGAGA AGAAAAATTT CTGTACAGTA CATTGAAAGT AGTGATTCAG 
     2221 AGGAAATCGA GACAAGCGAA CTGCCCCAGA AAATGAAAGG CAAGCTGAAA AATGTACAGT 
     2281 TAAATACTAA AAGTAGAGTA AAAGGATCAT CTAAGGTTAA GGAAGATTCA AAATTTGCAC 
     2341 TTACTTTTTT TGCCTCTGCA ACACAAAGGA AAATGTTGAA AAAAGGCATG TCTATGATGG 
     2401 AGTGCTCAGA GGCATGTGAC ACTGGAGAGA GGACAAGAGC AACACTGATC ATCTGTCCAC 
     2461 TCTCTGTGCT CAGCAACTGG ATTGACCAGT TTGGACAACA TGTAAAATCA GAAGTGCACT 
     2521 TGAATTTTTA TGTTTATTAT GGTCCTGATC GTATTAGAGA TTCAGCCTGG CTTTCAAAAC 
     2581 AGGATATTAT TTTGACTACT TACAATATTT TAACTCATGA TTATGGAACT AAAGATGATA 
     2641 GTCCATTGCA TAGCATCAAG TGGTTAAGGG TGATCCTGGA TGAAGGCCAT GCCATTCGAA 
     2701 ATCCAAACGC TCAGCAGACA AAAGCTGTAC TTGAGTTAGA GGCAGAAAGA AGATGGGTAT 
     2761 TGACAGGTAC TCCTATCCAG AATTCTTTAA AGGACTTGTG GTCTCTTCTG TCCTTCTTAA 
     2821 AACTTAAGCC ATTTATTGAT AGAGAGTGGT GGTATAGGAT CATACAGCGT CCTGTCACTA 
     2881 CAGGAGATGA GGGAGGACTT AGGCGTTTAC AGTCCTTAAT AAAAAATATC ACGCTTAGAA 
     2941 GAACAAAGAC AAGCAAAATT AAAGGGAAAC CTGTTTTGGA GTTGCCAGAA AGGAAAGTAT 
     3001 TTATTCAGCA CATTACTCTG TCAGAAGAAG AGAGAAAGAT TTATCAGTCT GTTAAAAATG 
     3061 AAGGCAAAGC TGCCATTGGA AGGTATTTTA CTGAAGGGAC TGTCCTTGCA CACTATGCAG 
     3121 ATGTCTTGGG TCTTCTGCTT AGATTGCGGC AGATTTGTTG CCATACCCAT CTTCTTACAA 
     3181 ACGGAATGTC TTCAAGTGGC CCCTCCAGAA GTGACACGCC TGAAGAGCTG AGAAAGATGT 
     3241 TAATAGAGAA GATGAAGATA ATTCTGAGCT CTGGTTCAGA TGAGGAATGT GCAATCTGCC 
     3301 TGGATTCCTT AACATTTCCT GTGATAACCC ACTGTGCACA CGTGTTCTGT AAACCTTGTA 
     3361 TCTGTCAGGT CATTCACAGT GAGCAGCCAC ATGCAAAGTG TCCTTTGTGC AGAAATGAGA 
     3421 TACATGGAGA TAATCTATTA GAATGTCCTC CCGAAGAATT GGCATGTGAC AGTGACAAAG 
     3481 AGTCCAGTAT GGAATGGAAG TCCAGCTCAA AGATTAATGC TCTAATGCAT GCTTTGATTG 
     3541 AATTACGGAC AAAGGACCCC AACATAAAAA GTTTAGTTGT TTCTCAGTTT ACCACATTCT 
     3601 TGTCTTTAAT AGAAACACCT CTTAAAGCCT CAGGATTTGT GTTTACTCGT TTAGATGGTT 
     3661 CCATGGCTCA AAAGAAAAGA GTTGAATCAA TTCAGCGTTT TCAAAACACA GAAGCAGGAT 
     3721 CTCCTACGAT AATGCTACTG TCCTTAAAAG CAGGTGGAGT TGGCTTGAAT CTCTGCGCAG 
     3781 CTTCTCGAGT GTTCTTAATG GATCCCGCCT GGAATCCTGC TGCAGAAGAT CAGTGCTTTG 
     3841 ACCGGTGCCA TCGACTTGGC CAGAAGCAAG AGGTGATCAT CACAAAATTC ATTGTGAAGG 
     3901 ACTCTGTGGA AGAAAATATG TTGAAAATAC AGAACACGAA GCGAGACCTT GCAGCTGGAG 
     3961 CCTTTGGCAC TAAGAAAACA GACGCCAATG ACATGAAACA AGCTAAAATC AACGAAATCA 
     4021 GAACTTTAAT TGACTTGACG CGTACGCGGC CGCTCGAGCA GAAACTCATC TCAGAAGAGG 
     4081 ATCTGGCAGC AAATGATATC CTGGATTACA AGGATGACGA CGATAAGGTT TAAACGGCCG 
     4141 GCCGCGGTCA TAGCTGTTTC CTGAACAGAT CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA 
     4201 GTGCCTCTCC TGGCCCTGGA AGTTGCCACT CCAGTGCCCA CCAGCCTTGT CCTAATAAAA 
     4261 TTAAGTTGCA TCATTTTGTC TGACTAGGTG TCCTTCTATA ATATTATGGG GTGGAGGGGG 
     4321 GTGGTATGGA GCAAGGGGCA AGTTGGGAAG ACAACCTGTA GGGCCTGCGG GGTCTATTGG 
     4381 GAACCAAGCT GGAGTGCAGT GGCACAATCT TGGCTCACTG CAATCTCCGC CTCCTGGGTT 
     4441 CAAGCGATTC TCCTGCCTCA GCCTCCCGAG TTGTTGGGAT TCCAGGCATG CATGACCAGG 
     4501 CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA CGGGGTTTCA CCATATTGGC CAGGCTGGTC 
     4561 TCCAACTCCT AATCTCAGGT GATCTACCCA CCTTGGCCTC CCAAATTGCT GGGATTACAG 
     4621 GCGTGAACCA CTGCTCCCTT CCCTGTCCTT CTGATTTTAA AATAACTATA CCAGCAGGAG 
     4681 GACGTCCAGA CACAGCATAG GCTACCTGGC CATGCCCAAC CGGTGGGACA TTTGAGTTGC 
     4741 TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG TCCACTCAGT AGATGCCTGT TGAATTGGGT 
     4801 ACGCGGCCAG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA 
     4861 ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG 
     4921 TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA 
     4981 AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT 
     5041 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT 
     5101 GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT 
     5161 CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC 
     5221 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT 
     5281 ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC 
     5341 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT 
     5401 CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA 
     5461 ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA 
     5521 AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA 
     5581 AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT 
     5641 TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAC CTGAGGCTAT 
     5701 GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC GAGCCCTGGG CCTTCACCCG AACTTGGGGG 
     5761 GTGGGGTGGG GAAAAGGAAG AAACGCGGGC GTATTGGCCC CAATGGGGTC TCGGTGGGGT 
     5821 ATCGACAGAG TGCCAGCCCT GGGACCGAAC CCCGCGTTTA TGAACAAACG ACCCAACACC 
     5881 GTGCGTTTTA TTCTGTCTTT TTATTGCCGT CATAGCGCGG GTTCCTTCCG GTATTGTCTC 
     5941 CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG GTGGGCGAAG AACTCCAGCA TGAGATCCCC 
     6001 GCGCTGGAGG ATCATCCAGC CGGCGTCCCG GAAAACGATT CCGAAGCCCA ACCTTTCATA 
     6061 GAAGGCGGCG GTGGAATCGA AATCTCGTGA TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT 
     6121 TTCGAACCCC AGAGTCCCGC TCAGAAGAAC TCGTCAAGAA GGCGATAGAA GGCGATGCGC 
     6181 TGCGAATCGG GAGCGGCGAT ACCGTAAAGC ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA 
     6241 AGCTCTTCAG CAATATCACG GGTAGCCAAC GCTATGTCCT GATAGCGATC CGCCACACCC 
     6301 AGCCGGCCAC AGTCGATGAA TCCAGAAAAG CGGCCATTTT CCACCATGAT ATTCGGCAAG 
     6361 CAGGCATCGC CATGGGTCAC GACGAGATCC TCGCCGTCGG GCATGCTCGC CTTGAGCCTG 
     6421 GCGAACAGTT CGGCTGGCGC GAGCCCCTGA TGCTCTTCGT CCAGATCATC CTGATCGACA 
     6481 AGACCGGCTT CCATCCGAGT ACGTGCTCGC TCGATGCGAT GTTTCGCTTG GTGGTCGAAT 
     6541 GGGCAGGTAG CCGGATCAAG CGTATGCAGC CGCCGCATTG CATCAGCCAT GATGGATACT 
     6601 TTCTCGGCAG GAGCAAGGTG AGATGACAGG AGATCCTGCC CCGGCACTTC GCCCAATAGC 
     6661 AGCCAGTCCC TTCCCGCTTC AGTGACAACG TCGAGCACAG CTGCGCAAGG AACGCCCGTC 
     6721 GTGGCCAGCC ACGATAGCCG CGCTGCCTCG TCTTGCAGTT CATTCAGGGC ACCGGACAGG 
     6781 TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC TGCGCTGACA GCCGGAACAC GGCGGCATCA 
     6841 GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA TAGCCGAATA GCCTCTCCAC CCAAGCGGCC 
     6901 GGAGAACCTG CGTGCAATCC ATCTTGTTCA ATCATGCGAA ACGATCCTCA TCCTGTCTCT 
     6961 TGATCGATCT TTGCAAAAGC CTAGGCCTCC AAAAAAGCCT CCTCACTACT TCTGGAATAG 
     7021 CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC ATAAATAAAA AAAATTAGTC AGCCATGGGG 
     7081 CGGAGAATGG GCGGAACTGG GCGGAGTTAG GGGCGGGATG GGCGGAGTTA GGGGCGGGAC 
     7141 TATGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     7201 GGACTTTCCA CACCTGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     7261 TGGGGAGCCT GGGGACTTTC CACACCCTAA CTGACACACA TTCCACAGCT GGTTCTTTCC 
     7321 GCCTCAGGAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG 
     7381 AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT 
     7441 CCCCGAAAAG TGCCACCTGA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG 
     7501 GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC 
     7561 TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC 
     7621 CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT 
     7681 GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG 
     7741 TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG 
     7801 GTCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG 
     7861 CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//