LOCUS       MR211401                7860 bp    DNA     circular  SYN 
DEFINITION  Ankrd27 (Myc-DDK-tagged) - Mouse ankyrin repeat domain 27 (VPS9 domain) (cDNA clone MGC:85985 IMAGE:6855501)
ACCESSION   BC065093
VERSION     BC065093
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4017..4108 
                     /label="Myc-DDK"  
     misc_feature    4138..4723 
                     /label="PolyA signal"  
     misc_feature    4195..4214 
                     /label="Restriction Enzyme"  
     misc_feature    4999..5671 
                     /label="ColE1"  
     misc_feature    6101..6895 
                     /label="Kan/Neo"  
     misc_feature    6930..7232 
                     /label="SV40 ori"  
     misc_feature    7318..7795 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3999..4005 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3999 
                     /label="Ankrd27"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCTCTATAT GATGAAGATC TCCTGAAAAA CCCTTTCTAC CTGGCCCTTC 
     1081 AGAAGTGGCG CCCCGACTTG TGCAGCAAGG TGGCCCAGAT CCATGGCATT GTCCTAGTGC 
     1141 CTTGCAGAGG AAGCCTGCCC GGCAGTGTGC AGGCCTCCTG CCAGTTCGAG TCCTATGTTT 
     1201 TGGTACCCAC GGAAGGACAC TTTCAGACCT TAGATGGAAA GGCTGTCGTC ATTGAAGGAA 
     1261 ACAGGATTAA GCTAGGAGCA GGTTTTGCTT GCCTTCTCTC TGTGCCCATC CTCTTTGAGG 
     1321 AGACTTTCTA CAACGAGAAA GAGGAGAGTT TCAGCATTCT CTGCATTGCT CATCCTTTGG 
     1381 AGAGGAGAGA GACTTCAGAA GAACCTTCAG CGCCTGCAGA TCCTTTCTCC CTGAAAACCA 
     1441 TCGAAGATGT GAGAGAATTT TTGGGAAGAC ACTCAGAGAA ATTTGACAAG AACATTGCCT 
     1501 CTTTCCACCG GACGTTCCGA GAATGTGAAA GAAAGAGCCT CCGCCACCAC ATAGACTCCG 
     1561 TAAATGCTCT CTACACCAAA TGCCTCCAGC AGCTTCTCCG AGACTCTCAC CTGAAGGTTC 
     1621 TTGCAAAGCA GGAGGCCCAG ATGAACCTGA TGAAGCAGGC CGTAGAGATG TATGTCCATC 
     1681 ACGATATTTA CGACCTGATC TTTAAATACG TGGGGACCAT GGAGGCGAGC GAGGATGCCG 
     1741 CCTTTAACAA AATCACGAGA AGCCTTCAAG ATCTCCAGCA GAAAGACATC GGCGTGAAAC 
     1801 CCGAGTTCAG CTTCAACATC CCTCGGGCAA AGAGAGAGCT GGGTCAGTTG AACAAGTGTA 
     1861 CGTCGCCACA GCAGAAGCTG CTGTGCCTGA GGAAGGTGGT CCAGCTCATG ACACAATCTC 
     1921 CCAGCCAGAG AGTGAACTTG GAGACCATGT GTGCCGATGA TCTCCTCTCG GTCCTGTTAT 
     1981 ATCTGCTCGT GAAGACAGAG ATCCCTAACT GGATGGCAAA TTTGAGCTAC ATCAAAAACT 
     2041 TCAGATTTAG CAGCTCGGCC AAAGATGAGC TAGGATACTG CCTGACCTCA GTCGAGGCTG 
     2101 CGATTGAATA CATCCGGCAA GGAAGTCTCT CCACGAAGAC GCCTGACGCT GAGGGATTTG 
     2161 GCGACAGACT ATTTCTCAAG CAGCGGATGA ACTTGCTGTC CCAGATGACA TCAACACCCA 
     2221 TCGACTGCCT GTTCAAGCAC ATCGCCTCTG GTAACCAAAA GGAAGTGGAA AGACTTCTGA 
     2281 GCCAAGATGA CCAGGACAAA GACGCCATGC AAAAGATGTG CCACCCACTT TGTTCCTGTG 
     2341 AGGACTGTGA GAAGCTTATC TCTGGGAGGC TGAATGACCC GTCAGTTGTC ACTCCTTTCT 
     2401 CCAGAGATGA CAGAGGTCAA ACACCCCTCC ATGTGGCTGC CCTCTGTGGT CAGGCATCCC 
     2461 TCATTGACTT CCTGGTCTCC AAAGGTGCCG TAGTGAACGC CACAGACTAT CATGGGTCCA 
     2521 CCCCCTTACA TCTGGCATGT CAGAAAGGCT TCCAAAGTGT GACGCTGCTG CTGCTGCACT 
     2581 ACAAGGCCAG CACTGAGGTT CAGGACAACA ACGGCAACAC CCCGCTGCAC CTGGCCTGCA 
     2641 CCTATGGGCA GGAGGATTGC GTGAAGGCCC TGGTCTACTA TGATGTCCAG GCCTGCAGAT 
     2701 TGGATATTGG TAATGAGAAA GGAGACACAG CCCTGCACAT TGCTGCACGC TGGGGTTACG 
     2761 AGGGCATCAT AGAGACACTG CTGCAGAATG GAGCCCCCAC AGCAGTCCAG AACAGACTGA 
     2821 AGGAAACACC ACTCAAGTGT GCTCTGAACT CGAAGATTCT GTCTATCATG GAAGCTCATC 
     2881 ACCTATCCTC TGACAGGAGG CCAAGGCCTT CTGAGGTTCC TGCACAGTCC CCTACACGCT 
     2941 CTGTGGATTC CATCAGCCAG GGCTCCTCCA CCTCCAGCTT TTCTTCCATA TCGGTGAGCT 
     3001 TCAGGCAAGA AGAGGTCAAA AAGGACTACA GGGAGAAACT GGCCAGGATT TCTGCCAATG 
     3061 GGCTTAGTGT GAATGTGACC AACCAGGACG GCTTCTCTCC CCTGCACATG GCTGCCCTGC 
     3121 ATGGCCGCAC AGACCTCGTC CCCCTCCTGC TGAAGCACGG TGCTTACTCA GGTGCCAGAA 
     3181 ACACAAGCCA AGCCGTCCCG CTCCACCTGG CCTGCCAGCA GGGGCACTTT CAGGTGGCAA 
     3241 AATGTCTCTT AGATTCCAAT GCAAAACCTA ATAAAAAGGA CCTCAGTGGG AACACACCCC 
     3301 TCATTTGTGC CTGCTCTGCC GGCCACCATG AAGTCGCTGC GCTGTTGCTA CAGCACGGGG 
     3361 CCTCCATCAA TGCTTGCAAC AACAAAGGCA ACACGGCCCT GCATGAGGCT GTGATGGGAA 
     3421 GGCACACCCT GGTAGTGGAG CTGCTTCTGT TCTACGGGGC GTCGGTTGAC ATCCTGAACA 
     3481 AGAGGCAGTA CACGGCGGCG GACTGTGCCG AGCAGGACTC AAAAATAATG GAGTTGCTGC 
     3541 AGGTAGTGCC AGGCTGTGTT GCGTCTCTAG ACAGCGTCGA GGAGGCAGAC CACGAGGGTT 
     3601 ACGTGACTGT TGAGATCAGG AGAAAATGGA ACCCCAAAAT GTACAACCTG CCAGAGGAAC 
     3661 CTCTTAGGAG ACAGTTTTGC CTCATTAACC CGGGGGGTCG GTTCCAGGAG AGGACATCCA 
     3721 GGGAGACTAT GGGAAGGGAT AGAAGTGTGC CCGATCTTGC AGGACGTTCT CTTCAGGAGC 
     3781 CAGAGAAGCA AAGAGTCACC GGAAAGCAGA GTGACCTGTC AGACCTGAGC AGGTATCAGA 
     3841 CCTCTGAGGA AGGAAACAAG GGGCTCCCGG AGAGGCCCGT GTCGAGACAG GCTGCTCCAG 
     3901 GACACCGGCC GATGGTCCGC AGACACACAG TCAATGACGC AGCCATACTC CAGGTCCCAG 
     3961 AGGTGACTGT CCACCTCACC ACCCACGAGG CGAGTGTTCC TCAGTCTACG CGTACGCGGC 
     4021 CGCTCGAGCA GAAACTCATC TCAGAAGAGG ATCTGGCAGC AAATGATATC CTGGATTACA 
     4081 AGGATGACGA CGATAAGGTT TAAACGGCCG GCCGCGGTCA TAGCTGTTTC CTGAACAGAT 
     4141 CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA GTGCCTCTCC TGGCCCTGGA AGTTGCCACT 
     4201 CCAGTGCCCA CCAGCCTTGT CCTAATAAAA TTAAGTTGCA TCATTTTGTC TGACTAGGTG 
     4261 TCCTTCTATA ATATTATGGG GTGGAGGGGG GTGGTATGGA GCAAGGGGCA AGTTGGGAAG 
     4321 ACAACCTGTA GGGCCTGCGG GGTCTATTGG GAACCAAGCT GGAGTGCAGT GGCACAATCT 
     4381 TGGCTCACTG CAATCTCCGC CTCCTGGGTT CAAGCGATTC TCCTGCCTCA GCCTCCCGAG 
     4441 TTGTTGGGAT TCCAGGCATG CATGACCAGG CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA 
     4501 CGGGGTTTCA CCATATTGGC CAGGCTGGTC TCCAACTCCT AATCTCAGGT GATCTACCCA 
     4561 CCTTGGCCTC CCAAATTGCT GGGATTACAG GCGTGAACCA CTGCTCCCTT CCCTGTCCTT 
     4621 CTGATTTTAA AATAACTATA CCAGCAGGAG GACGTCCAGA CACAGCATAG GCTACCTGGC 
     4681 CATGCCCAAC CGGTGGGACA TTTGAGTTGC TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG 
     4741 TCCACTCAGT AGATGCCTGT TGAATTGGGT ACGCGGCCAG CGGCGAGCGG TATCAGCTCA 
     4801 CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG 
     4861 AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA 
     4921 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA 
     4981 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC 
     5041 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC 
     5101 GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT 
     5161 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG 
     5221 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG 
     5281 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA 
     5341 CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG 
     5401 AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT 
     5461 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT 
     5521 TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG 
     5581 ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT 
     5641 CTAAAGTATA TATGAGTAAC CTGAGGCTAT GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC 
     5701 GAGCCCTGGG CCTTCACCCG AACTTGGGGG GTGGGGTGGG GAAAAGGAAG AAACGCGGGC 
     5761 GTATTGGCCC CAATGGGGTC TCGGTGGGGT ATCGACAGAG TGCCAGCCCT GGGACCGAAC 
     5821 CCCGCGTTTA TGAACAAACG ACCCAACACC GTGCGTTTTA TTCTGTCTTT TTATTGCCGT 
     5881 CATAGCGCGG GTTCCTTCCG GTATTGTCTC CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG 
     5941 GTGGGCGAAG AACTCCAGCA TGAGATCCCC GCGCTGGAGG ATCATCCAGC CGGCGTCCCG 
     6001 GAAAACGATT CCGAAGCCCA ACCTTTCATA GAAGGCGGCG GTGGAATCGA AATCTCGTGA 
     6061 TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT TTCGAACCCC AGAGTCCCGC TCAGAAGAAC 
     6121 TCGTCAAGAA GGCGATAGAA GGCGATGCGC TGCGAATCGG GAGCGGCGAT ACCGTAAAGC 
     6181 ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA AGCTCTTCAG CAATATCACG GGTAGCCAAC 
     6241 GCTATGTCCT GATAGCGATC CGCCACACCC AGCCGGCCAC AGTCGATGAA TCCAGAAAAG 
     6301 CGGCCATTTT CCACCATGAT ATTCGGCAAG CAGGCATCGC CATGGGTCAC GACGAGATCC 
     6361 TCGCCGTCGG GCATGCTCGC CTTGAGCCTG GCGAACAGTT CGGCTGGCGC GAGCCCCTGA 
     6421 TGCTCTTCGT CCAGATCATC CTGATCGACA AGACCGGCTT CCATCCGAGT ACGTGCTCGC 
     6481 TCGATGCGAT GTTTCGCTTG GTGGTCGAAT GGGCAGGTAG CCGGATCAAG CGTATGCAGC 
     6541 CGCCGCATTG CATCAGCCAT GATGGATACT TTCTCGGCAG GAGCAAGGTG AGATGACAGG 
     6601 AGATCCTGCC CCGGCACTTC GCCCAATAGC AGCCAGTCCC TTCCCGCTTC AGTGACAACG 
     6661 TCGAGCACAG CTGCGCAAGG AACGCCCGTC GTGGCCAGCC ACGATAGCCG CGCTGCCTCG 
     6721 TCTTGCAGTT CATTCAGGGC ACCGGACAGG TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC 
     6781 TGCGCTGACA GCCGGAACAC GGCGGCATCA GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA 
     6841 TAGCCGAATA GCCTCTCCAC CCAAGCGGCC GGAGAACCTG CGTGCAATCC ATCTTGTTCA 
     6901 ATCATGCGAA ACGATCCTCA TCCTGTCTCT TGATCGATCT TTGCAAAAGC CTAGGCCTCC 
     6961 AAAAAAGCCT CCTCACTACT TCTGGAATAG CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC 
     7021 ATAAATAAAA AAAATTAGTC AGCCATGGGG CGGAGAATGG GCGGAACTGG GCGGAGTTAG 
     7081 GGGCGGGATG GGCGGAGTTA GGGGCGGGAC TATGGTTGCT GACTAATTGA GATGCATGCT 
     7141 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCTGGTTG CTGACTAATT 
     7201 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCCTAA 
     7261 CTGACACACA TTCCACAGCT GGTTCTTTCC GCCTCAGGAC TCTTCCTTTT TCAATATTAT 
     7321 TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA 
     7381 AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA CGCGCCCTGT 
     7441 AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC 
     7501 AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC 
     7561 TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG 
     7621 CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA 
     7681 TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC 
     7741 CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT TTGATTTATA AGGGATTTTG 
     7801 CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTTT 
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