LOCUS       MR211386                7836 bp    DNA     circular  SYN 
DEFINITION  Pum2 (Myc-DDK-tagged) - Mouse pumilio 2 (Drosophila) (Pum2), transcript variant 5
ACCESSION   NM_001160222
VERSION     NM_001160222.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3993..4084 
                     /label="Myc-DDK"  
     misc_feature    4114..4699 
                     /label="PolyA signal"  
     misc_feature    4171..4190 
                     /label="Restriction Enzyme"  
     misc_feature    4975..5647 
                     /label="ColE1"  
     misc_feature    6077..6871 
                     /label="Kan/Neo"  
     misc_feature    6906..7208 
                     /label="SV40 ori"  
     misc_feature    7294..7771 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3975..3981 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3975 
                     /label="Pum2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAATCATGAT TTTCAAGCTC TTGCATTAGA ATCTCGGGGA ATGGGAGAGC 
     1081 TTTTGCCTAC CAAAAAGTTT TGGGAACCTG ATGATTCAAC AAAAGATGGA CAAAAAGGCA 
     1141 TATTTCTTGG GGATGATGAA TGGAGAGAGA CTGCATGGGG AACTTCTCAT CATTCAATGT 
     1201 CCCAGCCTAT TATGGTGCAG AGAAGATCTG GACAGAGTTT TCATGGAAAC AGTGAAGTAA 
     1261 ATGCAATTCT TTCTCCACGC TCAGAAAGTG GAGGCCTTGG TGTGAGCATG GTAGAATATG 
     1321 TATTAAGTTC CTCTCCTGCT GATAAATTGG ATTCTCGATT TAGGAAGGGA ACTTTTGGTA 
     1381 CTAGAGATGC TGAAACAGAT GGACCTGAGA AAGGAGATCA AAAAGGCAAG GCTTCTCCAT 
     1441 TTGAGGAGGA CCAAAACAGA GATCTTAAAC AAGATGATGA GGACTCTAAA ATAAATGGCA 
     1501 GAGGTTTGCC AAATGGAATG GATGCCGATT GCAAAGATTT TAATCGCACT CCTGGAAGTC 
     1561 GCCAAGCCTC TCCAACTGAA GTAGTTGAGC GCCTTGGCCC TAGTACTAAT CCCCCAGAAG 
     1621 GATTGGGCCC TCTTCCTAAT CCGACAGCGA ATAAACCACT TGTTGAAGAA TTTTCAAACC 
     1681 CTGAAACTCA GAATCTGGAT GCAATGGACC AAGTTGGTCT GGATTCCTTA CAGTTTGACT 
     1741 ATCCTGGTAA TCAGGTACCC ATGGATTCTT CAGGAGCTAC TGTAGGCCTT TTTGACTACA 
     1801 ATTCCCAACA GCAGCTCTTT CAGAGGACTA GTGCACTAAC AGTTCAGCAG TTAACTGCAG 
     1861 CTCAGCAGCA GCAGTATGCA TTAGCAGCAG CTCAGCAGCC ACATATAGCT GGTGTATTCT 
     1921 CAGCAGGCCT TGCTCCAGCT GCATTTGTGC CAAATCCATA TATTATTAGT GCTGCTCCTC 
     1981 CAGGGACTGA CCCGTATACT GCAGCAGGAT TGGCTGCAGC AGCAACATTA GCAGGTCCAG 
     2041 CAGTGGTTCC ACCTCAGTAT TACGGTGTTC CATGGGGAGT GTATCCAGCC AATTTATTTC 
     2101 AGCAACAAGC TGCAGCTGCG GCAAGCAACA CAGCCAACCA GCAAGCAGCA TCACAAGCTC 
     2161 AGCCTGGACA GCAGCAGGTT CTTCGTCCTG GAGCAGGTCA GCGTCCTATT ACTCCAAGTC 
     2221 AGGGCCAACA AGGGCAGCAA GCAGAGTCAC TTGCAGCAGC TGCAAACCCA ACTTTGGCTT 
     2281 TTGGTCAGAG TCTTGCTGCA GGCATGCCAG GCTATCAAGT ACTAGCTCCA ACTGCCTATT 
     2341 ATGATCAGAC TGGTGCCTTA GTGGTTGGCC CCGGAGCAAG AACTGGCCTT GGAGCTCCAG 
     2401 TACGATTAAT GGCTCCAACA CCTGTCTTAA TAAGTTCAAC AGCAGCACAA GCTGCAGCAG 
     2461 CAGCAGCAGC AGCTGGAGGA ACTGCAAATA GTCTTACAGG CAGCACAAAT GGTCTGTTTC 
     2521 GGCCAATTGG CACTCAGCCA CCACAGCAGC AGCAGCAGCA GCAGCAACCA AGCACTAACC 
     2581 TGCAGTCTAA TTCATTTTAT GGGAGCAGCT CTTTGACTAA CAGCTCCCAG AGCAGCTCTT 
     2641 TATTCTCTCA TGGACCTGGC CAACCTGGAA GTGCGTCTCT TGGCTTTGGA AGTGGTAGCT 
     2701 CTTTAGGAGC TGCTATAGGC TCAGCTCTCA GTGGATTTGG CTCATCAGGA GGACTGACAA 
     2761 ACGGTAGTGG TCGGTATATC TCTGCAGCAC CTGGAGCAGA AGCAAAGTAC CGAAGTGCTT 
     2821 CAAGCACTTC CAGTCTATTT AGCTCCAGCA GCCAGCTCTT TCCTCCTTCT CGGCTCCGCT 
     2881 ATAATAGATC TGACATCATG CCCTCCGGCC GCAGTAGGTT ATTGGAAGAT TTCAGGAACA 
     2941 ACCGCTTCCC CAACCTTCAG CTCAGAGACT TAATTGGACA TATAGTCGAG TTTTCTCAAG 
     3001 ACCAGCATGG TTCCAGATTC ATACAGCAAA AGCTAGAGAG AGCTACTCCA GCTGAGCGAC 
     3061 AAATAGTATT TAATGAAATT CTACAGGCAG CCTATCAATT AATGACAGAT GTTTTTGGAA 
     3121 ACTATGTTAT ACAGAAGTTT TTTGAGTTTG GAAGTTTGGA TCAGAAATTA GCCCTGGCTA 
     3181 CTCGTATTCG TGGTCATGTT CTACCATTAG CCTTGCAGAT GTATGGCTGC CGTGTTATTC 
     3241 AAAAGGCGTT AGAATCTATT TCTTCTGATC AGCAGAGTGA AATGGTTAAG GAACTAGATG 
     3301 GCCATGTACT TAAATGTGTG AAAGATCAAA ATGGAAACCA TGTTGTACAG AAATGCATTG 
     3361 AATGTGTTCA GCCACAGTCA CTGCAGTTCA TCATCGATGC TTTCAAAGGA CAAGTATTTG 
     3421 TGCTTTCAAC CCATCCTTAT GGCTGCAGAG TCATTCAGCG TATCTTAGAG CACTGCACGG 
     3481 CAGAGCAGAC CTTACCCATC TTAGAAGAAC TTCACCAACA CACAGAACAG TTGGTACAGG 
     3541 ATCAGTATGG CAATTATGTT ATTCAGCATG TACTGGAACA TGGTCGACCT GAAGACAAGA 
     3601 GCAAAATTGT TTCCGAAATC AGAGGAAAGG TCTTAGCCCT GAGTCAACAC AAATTTGCCA 
     3661 GCAATGTAGT AGAAAAGTGT GTTACTCATG CCTCCCGTGC TGAGAGAGCT TTACTGATTG 
     3721 ATGAGGTCTG CTGTCAGAAT GATGGTCCTC ACAGTGCCTT ATACACCATG ATGAAGGATC 
     3781 AGTATGCCAA CTATGTGGTT CAGAAGATGA TTGATATGGC TGAGCCTGCG CAGAGAAAGA 
     3841 TAATCATGCA CAAGATTCGA CCTCATATTA CTACTCTTCG CAAATACACA TATGGGAAGC 
     3901 ATATACTGGC CAAGTTGGAA AAATACTATC TGAAAAACAG CCCAGATCTA GGGCCAATTG 
     3961 GAGGACCACC AAATGGGATG CTGACGCGTA CGCGGCCGCT CGAGCAGAAA CTCATCTCAG 
     4021 AAGAGGATCT GGCAGCAAAT GATATCCTGG ATTACAAGGA TGACGACGAT AAGGTTTAAA 
     4081 CGGCCGGCCG CGGTCATAGC TGTTTCCTGA ACAGATCCCG GGTGGCATCC CTGTGACCCC 
     4141 TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT GCCACTCCAG TGCCCACCAG CCTTGTCCTA 
     4201 ATAAAATTAA GTTGCATCAT TTTGTCTGAC TAGGTGTCCT TCTATAATAT TATGGGGTGG 
     4261 AGGGGGGTGG TATGGAGCAA GGGGCAAGTT GGGAAGACAA CCTGTAGGGC CTGCGGGGTC 
     4321 TATTGGGAAC CAAGCTGGAG TGCAGTGGCA CAATCTTGGC TCACTGCAAT CTCCGCCTCC 
     4381 TGGGTTCAAG CGATTCTCCT GCCTCAGCCT CCCGAGTTGT TGGGATTCCA GGCATGCATG 
     4441 ACCAGGCTCA GCTAATTTTT GTTTTTTTGG TAGAGACGGG GTTTCACCAT ATTGGCCAGG 
     4501 CTGGTCTCCA ACTCCTAATC TCAGGTGATC TACCCACCTT GGCCTCCCAA ATTGCTGGGA 
     4561 TTACAGGCGT GAACCACTGC TCCCTTCCCT GTCCTTCTGA TTTTAAAATA ACTATACCAG 
     4621 CAGGAGGACG TCCAGACACA GCATAGGCTA CCTGGCCATG CCCAACCGGT GGGACATTTG 
     4681 AGTTGCTTGC TTGGCACTGT CCTCTCATGC GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA 
     4741 TTGGGTACGC GGCCAGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC 
     4801 CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG 
     4861 GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA 
     4921 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA 
     4981 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG 
     5041 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG 
     5101 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT 
     5161 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA 
     5221 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG 
     5281 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT 
     5341 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC 
     5401 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG 
     5461 CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG 
     5521 GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA 
     5581 GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAACCTGA 
     5641 GGCTATGGCA GGGCCTGCCG CCCCGACGTT GGCTGCGAGC CCTGGGCCTT CACCCGAACT 
     5701 TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG 
     5761 TGGGGTATCG ACAGAGTGCC AGCCCTGGGA CCGAACCCCG CGTTTATGAA CAAACGACCC 
     5821 AACACCGTGC GTTTTATTCT GTCTTTTTAT TGCCGTCATA GCGCGGGTTC CTTCCGGTAT 
     5881 TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC CCTAGGGTGG GCGAAGAACT CCAGCATGAG 
     5941 ATCCCCGCGC TGGAGGATCA TCCAGCCGGC GTCCCGGAAA ACGATTCCGA AGCCCAACCT 
     6001 TTCATAGAAG GCGGCGGTGG AATCGAAATC TCGTGATGGC AGGTTGGGCG TCGCTTGGTC 
     6061 GGTCATTTCG AACCCCAGAG TCCCGCTCAG AAGAACTCGT CAAGAAGGCG ATAGAAGGCG 
     6121 ATGCGCTGCG AATCGGGAGC GGCGATACCG TAAAGCACGA GGAAGCGGTC AGCCCATTCG 
     6181 CCGCCAAGCT CTTCAGCAAT ATCACGGGTA GCCAACGCTA TGTCCTGATA GCGATCCGCC 
     6241 ACACCCAGCC GGCCACAGTC GATGAATCCA GAAAAGCGGC CATTTTCCAC CATGATATTC 
     6301 GGCAAGCAGG CATCGCCATG GGTCACGACG AGATCCTCGC CGTCGGGCAT GCTCGCCTTG 
     6361 AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC CCCTGATGCT CTTCGTCCAG ATCATCCTGA 
     6421 TCGACAAGAC CGGCTTCCAT CCGAGTACGT GCTCGCTCGA TGCGATGTTT CGCTTGGTGG 
     6481 TCGAATGGGC AGGTAGCCGG ATCAAGCGTA TGCAGCCGCC GCATTGCATC AGCCATGATG 
     6541 GATACTTTCT CGGCAGGAGC AAGGTGAGAT GACAGGAGAT CCTGCCCCGG CACTTCGCCC 
     6601 AATAGCAGCC AGTCCCTTCC CGCTTCAGTG ACAACGTCGA GCACAGCTGC GCAAGGAACG 
     6661 CCCGTCGTGG CCAGCCACGA TAGCCGCGCT GCCTCGTCTT GCAGTTCATT CAGGGCACCG 
     6721 GACAGGTCGG TCTTGACAAA AAGAACCGGG CGCCCCTGCG CTGACAGCCG GAACACGGCG 
     6781 GCATCAGAGC AGCCGATTGT CTGTTGTGCC CAGTCATAGC CGAATAGCCT CTCCACCCAA 
     6841 GCGGCCGGAG AACCTGCGTG CAATCCATCT TGTTCAATCA TGCGAAACGA TCCTCATCCT 
     6901 GTCTCTTGAT CGATCTTTGC AAAAGCCTAG GCCTCCAAAA AAGCCTCCTC ACTACTTCTG 
     6961 GAATAGCTCA GAGGCCGAGG CGGCCTCGGC CTCTGCATAA ATAAAAAAAA TTAGTCAGCC 
     7021 ATGGGGCGGA GAATGGGCGG AACTGGGCGG AGTTAGGGGC GGGATGGGCG GAGTTAGGGG 
     7081 CGGGACTATG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     7141 GCCTGGGGAC TTTCCACACC TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     7201 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCCTAACTGA CACACATTCC ACAGCTGGTT 
     7261 CTTTCCGCCT CAGGACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT 
     7321 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC 
     7381 ACATTTCCCC GAAAAGTGCC ACCTGACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT 
     7441 GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC 
     7501 GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG 
     7561 GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT 
     7621 TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG 
     7681 TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT 
     7741 ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA 
     7801 AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTT
//