LOCUS       MR211381                7824 bp    DNA     circular  SYN 
DEFINITION  Usp15 (Myc-DDK-tagged) - Mouse ubiquitin specific peptidase 15 (Usp15)
ACCESSION   NM_027604
VERSION     NM_027604.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3981..4072 
                     /label="Myc-DDK"  
     misc_feature    4102..4687 
                     /label="PolyA signal"  
     misc_feature    4159..4178 
                     /label="Restriction Enzyme"  
     misc_feature    4963..5635 
                     /label="ColE1"  
     misc_feature    6065..6859 
                     /label="Kan/Neo"  
     misc_feature    6894..7196 
                     /label="SV40 ori"  
     misc_feature    7282..7759 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3963..3969 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3963 
                     /label="Usp15"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGGAAGGC GGAGCGGCGG ACCTGGACAC CCAGCGTTCT GACATCGCGA 
     1081 CGCTGCTCAA AACCTCGCTC CGGAAAGGGG ACACCTGGTA TCTAGTAGAT AGTCGGTGGT 
     1141 TCAAACAGTG GAAAAAATAT GTTGGCTTTG ACAGCTGGGA CAAATACCAG ATGGGAGATC 
     1201 AAAATGTCTA TCCTGGACCC ATCGATAACT CTGGACTTCT CAAAGATGGC GACGCGCAGT 
     1261 CACTTAAGGA GCACCTTATT GATGAGTTGG ATTACATACT CTTGCCAACT GAGGGCTGGA 
     1321 ATAAACTTGT CAGCTGGTAC ACACTGATGG AGGGCCAGGA GCCAATAGCA AGAAAGGTGG 
     1381 TAGAACAGGG TATGTTTGTA AAGCACTGCA AAGTAGAAGT CTACCTCACA GAACTGAAGC 
     1441 TCTGTGAGAA CGGGAACATG AACAATGTTG TAACTCGGAG GTTTAGCAAA GCTGACACAA 
     1501 TAGATACGAT TGAGAAGGAA ATAAGAAAAA TCTTCAATAT TCCAGATGAG AAGGAGGCCA 
     1561 GACTGTGGAA CAAGTATATG AGCAACACAT TTGAACCACT GAATAAGCCA GACAGCACCA 
     1621 TCCAGGATGC CGGCCTATAC CAAGGACAGG TGTTAGTGAT AGAGCAAAAA AATGAAGATG 
     1681 GAACGTGGCC CAGGGGTCCC TCTACTCCTA AGTCCCCAGG TGCATCCAAT TTTTCAACTT 
     1741 TACCAAAGAT CTCTCCTTCA TCTCTATCAA ATAATTATAA CAACATCAAC AACAGAAATG 
     1801 TGAAAAACTC AAATTATTGT CTCCCGTCAT ATACTGCTTA TAAGAACTAT GATTATTCAG 
     1861 AACCTGGAAG AAACAATGAA CAGCCAGGCC TGTGTGGCTT AAGTAACTTG GGAAATACGT 
     1921 GTTTCATGAA CTCAGCCATT CAGTGTTTGA GCAACACACC TCCACTTACT GAATATTTCC 
     1981 TCAATGATAA GTACCAAGAA GAGCTGAATT TTGACAATCC CTTAGGAATG AGAGGTGAAA 
     2041 TAGCTAAATC ATACGCTGAA CTCATCAAGC AGATGTGGTC TGGAAAGTTT AGCTACGTCA 
     2101 CTCCAAGAGC ATTCAAGACA CAGGTAGGGC GCTTCGCACC TCAGTTCTCT GGATATCAAC 
     2161 AGCAGGACTG CCAAGAACTA CTGGCTTTCC TGTTGGATGG ACTGCACGAG GACCTGAATA 
     2221 GAATTAGGAA AAAACCCTAT ATCCAGTTAA AAGATGCTGA TGGCAGGCCA GACAAGGTGG 
     2281 TTGCTGAAGA AGCCTGGGAG AACCATTTAA AGCGAAATGA TTCCATCATA GTAGACATAT 
     2341 TCCATGGCCT TTTCAAGTCT ACGTTAGTGT GTCCTGAGTG TGCTAAGATC TCGGTGACCT 
     2401 TTGACCCTTT CTGTTACTTG ACTCTTCCAT TGCCTATGAA AAAAGAACGT AGCTTGGAAG 
     2461 TTTATTTAGT TAGAATGGAT CCACTTGCTA AACCTATGCA ATACAAAGTT ATTGTGCCCA 
     2521 AAATTGGAAA TATACTAGAT CTTTGTACAG CGCTGTCTGC TTTGTCGGGA GTACCTGCAG 
     2581 ATAAGATGAT AGTCACTGAC ATATACAATC ATAGATTTCA CAGGATATTT GCCGTGGATG 
     2641 AAAACCTGAG TAGTATTATG GAGCGGGATG ATATTTACGT GTTTGAAATT AACATCAACA 
     2701 GGGCAGAAGA CACAGAGCAT GTAGTTATCC CTGTCTGCCT AAGAGAAAAA TTCAGACACT 
     2761 CAAGTTACAC TCACCACACT GGCTCTTCAC TGTTCGGCCA GCCTTTTCTT ATGGCTATAC 
     2821 CACGAAACAA TACTGAAGAT AAACTCTATA ATCTTCTACT CTTGAGAATG TGCCGGTATG 
     2881 TCAAAATGTC TACTGAAACT GAGGAAACAG ATGGACACCT ACGTTGCTGT GAGGACCAGA 
     2941 ATATTAATGG GAATGGGCCA AATGGCCTAC ATGAAGAGGG TTCACCAAGT GAGATGGAAA 
     3001 CCGACGAGCC AGATGACGAG TCCAGCCAGG ATCAAGAGCT TCCCTCAGAG AACGAGAACA 
     3061 GTCAGTCTGA AGATTCAGTC GGAGGAGATA ACGATTCTGA AAATGGATTG TGCACTGAAG 
     3121 AAACATGCAA AGGTCAACTT ACGGGACACA AAAAGCGCTT GTTTACATTC CAGTTCAACA 
     3181 ACTTAGGCAA CAATGACATC AACTACATCA AAGACGACAC CAGTCACATC CGATTCGATG 
     3241 ACAGGCAGCT TCGGCTGGAC GAAAGATCTT TTCTCGCTTT GGACTGGGAC CCTGACTTGA 
     3301 AGAAGAGATA CTTTGATGAG AATGCTGCAG AGGATTTTGA AAAACATGAA AGTGTGGAAT 
     3361 ACAAACCTCC AAAAAGGCCC TTTGTGAAGT TAAAAGACTG CATTGAGCTC TTCACCACCA 
     3421 AGGAGAAGCT GGGTGCTGAG GACCCCTGGT ATTGTCCAAA TTGTAAAGAG CATCAGCAAG 
     3481 CCACAAAGAA ATTAGATCTG TGGTCCCTGC CTCCAGTTCT TGTGGTGCAT CTCAAGAGAT 
     3541 TCTCCTATAG TCGCTACATG AGAGACAAGC TAGATACATT AGTCGATTTT CCCATCAGTG 
     3601 ACTTGGATAT GTCAGAATTC CTAATTAACC CAAATGCAGG TCCTTGCCGC TATAATTTGA 
     3661 TTGCTGTTTC CAACCACTAT GGAGGGATGG GAGGAGGACA TTATACTGCT TTTGCAAAAA 
     3721 ACAAAGATGA TGGGAAGTGG TACTATTTCG ATGACAGTAG CGTCTCCACT GCATCTGAAG 
     3781 ACCAAATCGT GTCCAAGGCA GCGTACGTGC TCTTCTACCA GAGACAGGAC ACTTTCAGTG 
     3841 GGACTGGCTT CTTCCCTCTT GACCGAGAAA CTAAAGGTGC TTCAGCTGCC ACAGGTATCC 
     3901 CCCTGGAAAG TGACGAAGAC AGCAATGATA ATGACAATGA CCTAGAAAAT GAAAACTGTA 
     3961 TGCACACTAA TACGCGTACG CGGCCGCTCG AGCAGAAACT CATCTCAGAA GAGGATCTGG 
     4021 CAGCAAATGA TATCCTGGAT TACAAGGATG ACGACGATAA GGTTTAAACG GCCGGCCGCG 
     4081 GTCATAGCTG TTTCCTGAAC AGATCCCGGG TGGCATCCCT GTGACCCCTC CCCAGTGCCT 
     4141 CTCCTGGCCC TGGAAGTTGC CACTCCAGTG CCCACCAGCC TTGTCCTAAT AAAATTAAGT 
     4201 TGCATCATTT TGTCTGACTA GGTGTCCTTC TATAATATTA TGGGGTGGAG GGGGGTGGTA 
     4261 TGGAGCAAGG GGCAAGTTGG GAAGACAACC TGTAGGGCCT GCGGGGTCTA TTGGGAACCA 
     4321 AGCTGGAGTG CAGTGGCACA ATCTTGGCTC ACTGCAATCT CCGCCTCCTG GGTTCAAGCG 
     4381 ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG GGATTCCAGG CATGCATGAC CAGGCTCAGC 
     4441 TAATTTTTGT TTTTTTGGTA GAGACGGGGT TTCACCATAT TGGCCAGGCT GGTCTCCAAC 
     4501 TCCTAATCTC AGGTGATCTA CCCACCTTGG CCTCCCAAAT TGCTGGGATT ACAGGCGTGA 
     4561 ACCACTGCTC CCTTCCCTGT CCTTCTGATT TTAAAATAAC TATACCAGCA GGAGGACGTC 
     4621 CAGACACAGC ATAGGCTACC TGGCCATGCC CAACCGGTGG GACATTTGAG TTGCTTGCTT 
     4681 GGCACTGTCC TCTCATGCGT TGGGTCCACT CAGTAGATGC CTGTTGAATT GGGTACGCGG 
     4741 CCAGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG 
     4801 GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA 
     4861 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG 
     4921 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC 
     4981 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC 
     5041 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC 
     5101 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG 
     5161 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC 
     5221 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA 
     5281 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC 
     5341 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC 
     5401 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG 
     5461 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC 
     5521 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA 
     5581 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAACCTGAGG CTATGGCAGG 
     5641 GCCTGCCGCC CCGACGTTGG CTGCGAGCCC TGGGCCTTCA CCCGAACTTG GGGGGTGGGG 
     5701 TGGGGAAAAG GAAGAAACGC GGGCGTATTG GCCCCAATGG GGTCTCGGTG GGGTATCGAC 
     5761 AGAGTGCCAG CCCTGGGACC GAACCCCGCG TTTATGAACA AACGACCCAA CACCGTGCGT 
     5821 TTTATTCTGT CTTTTTATTG CCGTCATAGC GCGGGTTCCT TCCGGTATTG TCTCCTTCCG 
     5881 TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC GAAGAACTCC AGCATGAGAT CCCCGCGCTG 
     5941 GAGGATCATC CAGCCGGCGT CCCGGAAAAC GATTCCGAAG CCCAACCTTT CATAGAAGGC 
     6001 GGCGGTGGAA TCGAAATCTC GTGATGGCAG GTTGGGCGTC GCTTGGTCGG TCATTTCGAA 
     6061 CCCCAGAGTC CCGCTCAGAA GAACTCGTCA AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA 
     6121 TCGGGAGCGG CGATACCGTA AAGCACGAGG AAGCGGTCAG CCCATTCGCC GCCAAGCTCT 
     6181 TCAGCAATAT CACGGGTAGC CAACGCTATG TCCTGATAGC GATCCGCCAC ACCCAGCCGG 
     6241 CCACAGTCGA TGAATCCAGA AAAGCGGCCA TTTTCCACCA TGATATTCGG CAAGCAGGCA 
     6301 TCGCCATGGG TCACGACGAG ATCCTCGCCG TCGGGCATGC TCGCCTTGAG CCTGGCGAAC 
     6361 AGTTCGGCTG GCGCGAGCCC CTGATGCTCT TCGTCCAGAT CATCCTGATC GACAAGACCG 
     6421 GCTTCCATCC GAGTACGTGC TCGCTCGATG CGATGTTTCG CTTGGTGGTC GAATGGGCAG 
     6481 GTAGCCGGAT CAAGCGTATG CAGCCGCCGC ATTGCATCAG CCATGATGGA TACTTTCTCG 
     6541 GCAGGAGCAA GGTGAGATGA CAGGAGATCC TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG 
     6601 TCCCTTCCCG CTTCAGTGAC AACGTCGAGC ACAGCTGCGC AAGGAACGCC CGTCGTGGCC 
     6661 AGCCACGATA GCCGCGCTGC CTCGTCTTGC AGTTCATTCA GGGCACCGGA CAGGTCGGTC 
     6721 TTGACAAAAA GAACCGGGCG CCCCTGCGCT GACAGCCGGA ACACGGCGGC ATCAGAGCAG 
     6781 CCGATTGTCT GTTGTGCCCA GTCATAGCCG AATAGCCTCT CCACCCAAGC GGCCGGAGAA 
     6841 CCTGCGTGCA ATCCATCTTG TTCAATCATG CGAAACGATC CTCATCCTGT CTCTTGATCG 
     6901 ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA GCCTCCTCAC TACTTCTGGA ATAGCTCAGA 
     6961 GGCCGAGGCG GCCTCGGCCT CTGCATAAAT AAAAAAAATT AGTCAGCCAT GGGGCGGAGA 
     7021 ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG GATGGGCGGA GTTAGGGGCG GGACTATGGT 
     7081 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     7141 TCCACACCTG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     7201 GCCTGGGGAC TTTCCACACC CTAACTGACA CACATTCCAC AGCTGGTTCT TTCCGCCTCA 
     7261 GGACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA 
     7321 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA 
     7381 AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG 
     7441 CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT 
     7501 TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA 
     7561 GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT 
     7621 TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG 
     7681 TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT 
     7741 TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT 
     7801 TAACAAAAAT TTAACGCGAA TTTT
//