LOCUS       MR211352                7800 bp    DNA     circular  SYN 
DEFINITION  Rnf20 (Myc-DDK-tagged) - Mouse ring finger protein 20 (Rnf20), transcript variant 1
ACCESSION   NM_001163263
VERSION     NM_001163263.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3957..4048 
                     /label="Myc-DDK"  
     misc_feature    4078..4663 
                     /label="PolyA signal"  
     misc_feature    4135..4154 
                     /label="Restriction Enzyme"  
     misc_feature    4939..5611 
                     /label="ColE1"  
     misc_feature    6041..6835 
                     /label="Kan/Neo"  
     misc_feature    6870..7172 
                     /label="SV40 ori"  
     misc_feature    7258..7735 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3939..3945 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3939 
                     /label="Rnf20"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCAGGAATT GGAAATAAAA GAGCAGCTGG AGAGCCGGGC ACATCTATGC 
     1081 CTCCAGAGAA GAAGACAGCT GTTGAAGATT CAGGGACCAC CGTGGAAACA ATTAAGTTAG 
     1141 GAGGTGTCTC TTCAACGGAG GAATTAGACA TTCGAACACT GCAATCCAAG AATCGTAAGC 
     1201 TAGCAGAAAT GCTAGATCAG CGGCAGGCCA TTGAAGATGA GCTTCGGGAA CACATTGAAA 
     1261 AGCTGGAGCG ACGCCAGGCC ACTGATGATG CCTCACTGTT GATTGTGAAC CGGTACTGGA 
     1321 GCCAGTTTGA TGAAAACATC CGCATCATCC TTAAACGTTA TGATCTGGAC CAGGGTTTGG 
     1381 GGGACCTACT CACAGAAAGG AAAGCCTTGG TTGTGCCAGA ACCAGAACCT GACTCTGATA 
     1441 GCAATCAGGA ACGCAAAGAT GACCGTGAGA GAGGGGATGG GCAAGAGCCA GCTTTCTCTT 
     1501 TCCTTGCTAC TTTGGCTAGC AGTTCCAGTG AAGAGATGGA ATCTCAGCTT CAGGAGCGTG 
     1561 TAGAGTCCTC CCGCCGTGCT GTGTCCCAGA TTGTGACTGT CTATGATAAA TTGCAAGAAA 
     1621 AAGTGGATCT CTTATCCCGG AAACTGAACA GTGGAGATAA CCTGATAGTG GAGGAAGCAG 
     1681 TGCAGGAGTT GAACTCCTTC CTTGCACAAG AGAATGTGAG GCTTCAGGAG CTGACAGACC 
     1741 TCCTTCAGGA GAAGCATCAC ACCATGTCTC AGGAGTTCTG TAAGTTGCAG GGTAAAGTAG 
     1801 AGACAGCTGA GTCACGAGTG TCCGTCTTGG AGTCCATGAT TGATGACCTG CAGTGGGATA 
     1861 TTGACAAAAT TCGAAAAAGA GAACAGCGTC TCAACCGGCA TTTGGCTGAA GTCCTAGAGC 
     1921 GGGTGAATTC AAAAGGCTAT AAAGTATATG GAGCGGGGAG CAGTCTCTAT GGAGGCACAA 
     1981 TCACCATCAA TGCCCGGAAG TTTGAGGAAA TGAATGCAGA GCTTGAGGAG AACAAAGAGT 
     2041 TGGCTCAGAA CCGACATTGT GAGCTGGAGA AGCTTCGTCA AGACTTTGAA GAGGTCACTA 
     2101 CACAGAATGA AAAGTTGAAG GTGGAATTGC GGAGTGCAGT GGAGGAAGTG GTTAAGGAAA 
     2161 CCCCAGAGTA TCGCTGCATG CAGTCACAGT TCTCTGTCCT GTATAACGAG AGTCTGCAGT 
     2221 TGAAAGCCCA CTTGGATGAA GCTCGGACCC TGCTTCATGG CACCCGGGGT ACTCACCAGC 
     2281 GCCAGGTTGA ACTCATTGAG CGAGATGAGG TTAGTCTGCA TAAGAAGCTG AGAACTGAAG 
     2341 TCATTCAGCT GGAGGATACC TTGGCCCAGG TTCGCAAGGA GTATGAGATG TTGAGGATAG 
     2401 AGTTTGAGCA GACCCTTGCT GCCAATGAAC AAGCAGGCCC CATAAACCGG GAGATGCGGC 
     2461 ATCTCATTAG CAGCCTCCAG AATCATAACC ACCAGCTGAA GGGTGAAGTC CTAAGGTATA 
     2521 AACGGAAGCT GAGAGAGGCG CAGTCTGACC TGAACAAGAC ACGTCTGCGC AGTGGCAGTG 
     2581 CCCTCCTGCA GTCTCAGTCT AGTACTGAGG ACCCCAAGGA CGAGCCCACA GAGCTGAAGC 
     2641 AGGATTCTGA AGACCTAGCC ACTCATTCCT CAGCGCTGAA GGCATCTCAG GAGGATGAAG 
     2701 TTAAGTCCAA ACGGGATGAA GAAGAGAGAG AACGAGAAAG GAGGGAGAAA GAAAGAGAGC 
     2761 GAGAGCGAGA ACGAGAAAAG GAAAAGGAGA GAGAACGAGA GAAACAGAAA CTGAAAGAGT 
     2821 CAGAAAAGGA GAGAGATTCT GTTAAGGATA AAGAGAAAGG GAAACATGAT GATGGAAGGA 
     2881 AGAAGGAAGC AGAAATTATC AAGCAGCTGA AGATTGAACT TAAGAAAGCA CAAGAGAGCC 
     2941 AGAAGGAGAT GAAACTACTG CTGGATATGT ACCGCTCTGC CCCCAAGGAG CAGAGAGACA 
     3001 AAGTGCAGCT AATGGCAGCT GAGAAGAAGT CAAAGGCAGA GTTGGAGGAT CTCAGACAAA 
     3061 GACTCAAGGA TCTGGAAGAT AAGGAGAAGA AAGAGAACAA GAAAATGGCT GATGAGGATG 
     3121 CCTTGAGGAA GATCCGGGCA GTGGAGGAGC AGATTGAATA CCTGCAGAAG AAGCTGGCTA 
     3181 TGGCCAAGCA GGAGGAAGAG GCTCTCCTGT CTGAGATGGA TGTCACAGGA CAGGCCTTTG 
     3241 AGGACATGCA GGAGCAGAAC ATCCGTCTGA TGCAGCAGTT GCGGGAGAAG GACGATGCAA 
     3301 ACTTCAAGCT CATGTCAGAG CGAATCAAGT CTAATCAGAT CCATAAGTTG CTTAAAGAGG 
     3361 AAAAGGAAGA GTTAGCTGAT CAGGTTTTGA CACTGAAGAC TCAGGTAGAT GCCCAGTTAC 
     3421 AGGTAGTAAG AAAACTGGAA GAGAAGGAGC ACCTACTTCA GAGTAACATT GGCACAGGGG 
     3481 AGAAGGAGCT GGGTCTTAGG ACTCAAGCCT TGGAGATGAA TAAACGCAAG GCAATGGAGG 
     3541 CGGCCCAGCT TGCAGATGAC CTCAAAGCAC AGCTAGAACT GGCTCAGAAG AAGCTCCATG 
     3601 ATTTCCAGGA TGAAATCGTG GAGAACAGTG TCACCAAAGA AAAGGACTTG TTCAATTTCA 
     3661 AACGAGCACA GGAAGACATC TCTAGGCTTC GAAGAAAGCT AGAGACCACC AAGAAGCCAG 
     3721 ACAATGTGCC CAAGTGTGAT GAAATTCTGA TGGAGGAGAT AAAGGACTAC AAGGCACGGC 
     3781 TGACCTGCCC TTGCTGTAAC ATGCGTAAAA AGGATGCAGT ACTTACCAAG TGTTTTCACG 
     3841 TTTTCTGCTT TGAGTGTGTG AAGACGCGCT ATGACACTCG CCAGCGCAAG TGTCCCAAGT 
     3901 GTAATGCTGC TTTTGGTGCC AATGATTTCC ATCGCATCTA CATTGGCACG CGTACGCGGC 
     3961 CGCTCGAGCA GAAACTCATC TCAGAAGAGG ATCTGGCAGC AAATGATATC CTGGATTACA 
     4021 AGGATGACGA CGATAAGGTT TAAACGGCCG GCCGCGGTCA TAGCTGTTTC CTGAACAGAT 
     4081 CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA GTGCCTCTCC TGGCCCTGGA AGTTGCCACT 
     4141 CCAGTGCCCA CCAGCCTTGT CCTAATAAAA TTAAGTTGCA TCATTTTGTC TGACTAGGTG 
     4201 TCCTTCTATA ATATTATGGG GTGGAGGGGG GTGGTATGGA GCAAGGGGCA AGTTGGGAAG 
     4261 ACAACCTGTA GGGCCTGCGG GGTCTATTGG GAACCAAGCT GGAGTGCAGT GGCACAATCT 
     4321 TGGCTCACTG CAATCTCCGC CTCCTGGGTT CAAGCGATTC TCCTGCCTCA GCCTCCCGAG 
     4381 TTGTTGGGAT TCCAGGCATG CATGACCAGG CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA 
     4441 CGGGGTTTCA CCATATTGGC CAGGCTGGTC TCCAACTCCT AATCTCAGGT GATCTACCCA 
     4501 CCTTGGCCTC CCAAATTGCT GGGATTACAG GCGTGAACCA CTGCTCCCTT CCCTGTCCTT 
     4561 CTGATTTTAA AATAACTATA CCAGCAGGAG GACGTCCAGA CACAGCATAG GCTACCTGGC 
     4621 CATGCCCAAC CGGTGGGACA TTTGAGTTGC TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG 
     4681 TCCACTCAGT AGATGCCTGT TGAATTGGGT ACGCGGCCAG CGGCGAGCGG TATCAGCTCA 
     4741 CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG 
     4801 AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA 
     4861 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA 
     4921 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC 
     4981 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC 
     5041 GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT 
     5101 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG 
     5161 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG 
     5221 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA 
     5281 CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG 
     5341 AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT 
     5401 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT 
     5461 TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG 
     5521 ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT 
     5581 CTAAAGTATA TATGAGTAAC CTGAGGCTAT GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC 
     5641 GAGCCCTGGG CCTTCACCCG AACTTGGGGG GTGGGGTGGG GAAAAGGAAG AAACGCGGGC 
     5701 GTATTGGCCC CAATGGGGTC TCGGTGGGGT ATCGACAGAG TGCCAGCCCT GGGACCGAAC 
     5761 CCCGCGTTTA TGAACAAACG ACCCAACACC GTGCGTTTTA TTCTGTCTTT TTATTGCCGT 
     5821 CATAGCGCGG GTTCCTTCCG GTATTGTCTC CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG 
     5881 GTGGGCGAAG AACTCCAGCA TGAGATCCCC GCGCTGGAGG ATCATCCAGC CGGCGTCCCG 
     5941 GAAAACGATT CCGAAGCCCA ACCTTTCATA GAAGGCGGCG GTGGAATCGA AATCTCGTGA 
     6001 TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT TTCGAACCCC AGAGTCCCGC TCAGAAGAAC 
     6061 TCGTCAAGAA GGCGATAGAA GGCGATGCGC TGCGAATCGG GAGCGGCGAT ACCGTAAAGC 
     6121 ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA AGCTCTTCAG CAATATCACG GGTAGCCAAC 
     6181 GCTATGTCCT GATAGCGATC CGCCACACCC AGCCGGCCAC AGTCGATGAA TCCAGAAAAG 
     6241 CGGCCATTTT CCACCATGAT ATTCGGCAAG CAGGCATCGC CATGGGTCAC GACGAGATCC 
     6301 TCGCCGTCGG GCATGCTCGC CTTGAGCCTG GCGAACAGTT CGGCTGGCGC GAGCCCCTGA 
     6361 TGCTCTTCGT CCAGATCATC CTGATCGACA AGACCGGCTT CCATCCGAGT ACGTGCTCGC 
     6421 TCGATGCGAT GTTTCGCTTG GTGGTCGAAT GGGCAGGTAG CCGGATCAAG CGTATGCAGC 
     6481 CGCCGCATTG CATCAGCCAT GATGGATACT TTCTCGGCAG GAGCAAGGTG AGATGACAGG 
     6541 AGATCCTGCC CCGGCACTTC GCCCAATAGC AGCCAGTCCC TTCCCGCTTC AGTGACAACG 
     6601 TCGAGCACAG CTGCGCAAGG AACGCCCGTC GTGGCCAGCC ACGATAGCCG CGCTGCCTCG 
     6661 TCTTGCAGTT CATTCAGGGC ACCGGACAGG TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC 
     6721 TGCGCTGACA GCCGGAACAC GGCGGCATCA GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA 
     6781 TAGCCGAATA GCCTCTCCAC CCAAGCGGCC GGAGAACCTG CGTGCAATCC ATCTTGTTCA 
     6841 ATCATGCGAA ACGATCCTCA TCCTGTCTCT TGATCGATCT TTGCAAAAGC CTAGGCCTCC 
     6901 AAAAAAGCCT CCTCACTACT TCTGGAATAG CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC 
     6961 ATAAATAAAA AAAATTAGTC AGCCATGGGG CGGAGAATGG GCGGAACTGG GCGGAGTTAG 
     7021 GGGCGGGATG GGCGGAGTTA GGGGCGGGAC TATGGTTGCT GACTAATTGA GATGCATGCT 
     7081 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCTGGTTG CTGACTAATT 
     7141 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCCTAA 
     7201 CTGACACACA TTCCACAGCT GGTTCTTTCC GCCTCAGGAC TCTTCCTTTT TCAATATTAT 
     7261 TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA 
     7321 AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA CGCGCCCTGT 
     7381 AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC 
     7441 AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC 
     7501 TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG 
     7561 CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA 
     7621 TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC 
     7681 CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT TTGATTTATA AGGGATTTTG 
     7741 CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//