LOCUS       MR211337                7788 bp    DNA     circular  SYN 
DEFINITION  Pkd2 (Myc-DDK-tagged) - Mouse polycystic kidney disease 2 (Pkd2)
ACCESSION   NM_008861
VERSION     NM_008861.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3943..4034 
                     /label="Myc-DDK"  
     misc_feature    4064..4649 
                     /label="PolyA signal"  
     misc_feature    4121..4140 
                     /label="Restriction Enzyme"  
     misc_feature    4925..5597 
                     /label="ColE1"  
     misc_feature    6027..6821 
                     /label="Kan/Neo"  
     misc_feature    6856..7158 
                     /label="SV40 ori"  
     misc_feature    7244..7721 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3918..3925 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3918 
                     /label="Pkd2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGTTAACTCC AGACGCGTGC AGCCGCAGCC GCCCGGGGAC GCGGGACGCT 
     1081 CGCCCGCGCC GCGAGCGTCC GGACCCGGGC GCCTGGTGGC GGGAGGCGCC GGCCTAGCTG 
     1141 TCCCCGGCGG CCTCGGGGAG CAGCGGGGCC TGGAGATCGA GATGGAGCGC ATCCGGCAGG 
     1201 CGGCCGCTCG GGATCCCCCG GCCGGAGCCT CGGCCTCGCC GTCTCCTCCG CTTTCGTCCT 
     1261 GCTCCAGGCA AGCGTGGAGC CGCGACAACC CGGGCTTTGA GGCAGAGGAG GATGACGACG 
     1321 ACGACGAGGT GGAAGGAGAA GAAGGAGGGA TGGTGGTAGA GATGGATGTG GAGTGGCGCC 
     1381 CGGGCAGTCG GAGGTCGGCC TCCTCCTCGG CCGTGAGCTC GGTGGGCGCC CGCGGCCGAG 
     1441 GGCTCGGGAG CTACCGCGGC GCGGCTCACC TGAGCGGGAG GCGGCGCCGG CTAGAGGACC 
     1501 AGGGCGCGCA GTGTCCCAGC CCCGCGGGCG GCGGGGACCC GCTGCATCGC CACCTCCCGC 
     1561 TGGAGGGCCA GCCACCCCGA GTGGCCTGGG CAGAGAGGCT GGTGCGAGGG CTGCGAGGTC 
     1621 TCTGGGGAAC AAGACTCATG GAAGAGAGCA ACGCCAACCG AGAGAAGTAC CTGAAAAGTG 
     1681 TGTTACGGGA GCTGGTCACT TACCTCTTTT TCCTCGTAGT CTTGTGCATC TTGACCTACG 
     1741 GCATGATGAG CTCCAATGTG TACTACTACA CTCGGACACT GTCACAGCTA TTCATAGACA 
     1801 CCCCAGTGTC GAAAACAGAG AAAACCAACT TTAAAACTCT TTCTTCCATG GAGGACTTCT 
     1861 GGAAGTTCAC CGAAGGCTCC TTCCTGGATG GGCTGTACTG GAAGGCACAG ACCAGCAACC 
     1921 ACACGCAAGC TGACAACCGA AGCTTTATCT TCTATGAGAA CCTGCTGCTA GGAGTGCCGC 
     1981 GTCTACGCCA ACTCCGAGTC AGAAACGGAT CCTGCTCCAT CCCTCAGGAC CTGCGAGATG 
     2041 AAATTAAAGA GTGCTATGAC GTCTACTCCG TCAGCAGTGA GGACAGAGCT CCATTTGGAC 
     2101 CGCGGAATGG AACTGCGTGG ATGTACACAA GTGAGAAGGA GCTGAATGGG AGCAGTCACT 
     2161 GGGGGATCAT TGCGTCGTAC AGTGGAGCGG GTTACTACCT GGATCTGTCC AGAACCAGGG 
     2221 AGGAGACAGC AGCCCAGCTT GCTGGCCTCA GGAGGAACTT CTGGCTGGAC CGGGGCACGC 
     2281 GGGCAGCTTT TATAGACTTC TCGGTGTATA ACGCAAACAT TAACCTGTTC TGTGTGGTCA 
     2341 GGTTATTGGC GGAGTTCCCA GCAACGGGTG GCGTGGTACC CTCTTGGCAG TTTCAGCCTG 
     2401 TAAAACTGAT CCGCTATGTC ACAGCCTTTG ATTTCTTCCT GGCAGCCTGT GAGATCATCT 
     2461 TTTGTTTCTT TATCATTTAC TATGTGGTGG AAGAGATATT GGAAATTCGG ATTCACAGAC 
     2521 TGAGCTATTT CAGGAGTTTC TGGAATTGTC TGGATGTTGT GATTGTCGTG TTATCTGTAG 
     2581 TAGCTATGGT GATTAACATT TACCGAATGT CAAATGCAGA GGGGCTGCTA CAGTTTCTTG 
     2641 AAGATCAAAA TTCTTTCCCC AACTTTGAGC ATGTGGCATA CTGGCAAATA CAGTTCAACA 
     2701 ATATAAGTGC TGTCATGGTA TTTTTGGTCT GGATTAAGCT CTTCAAATTC ATCAATTTTA 
     2761 ATAGGACCAT GAGCCAGCTC TCCACAACCA TGTCTCGATG TGCCAAAGAC CTCTTCGGCT 
     2821 TCACCATAAT GTTCTCCATC ATCTTCTTGG CATACGCACA GCTGGCATAC CTTGTCTTCG 
     2881 GCACCCAGGT CGATGACTTC AGCACTTTCC AAGAATGTAT CTTCACCCAG TTCCGCATCA 
     2941 TTTTGGGTGA TATCAACTTC GCAGAGATCG AGGAAGCTAA CCGAGTTTTG GGGCCACTTT 
     3001 ATTTTACTAC ATTTGTGTTC TTTATGTTCT TCATTCTTTT GAATATGTTC CTGGCGATCA 
     3061 TCAATGATTC GTACTCTGAA GTGAAATCCG ATCTGGCCCA GCAGAAAGCA GAAATGGAAC 
     3121 TCTCAGACCT TATCAGAAAG GGCTGCCAAA AAGCACTGGT CAAACTAAAA CTGAAAAGAA 
     3181 ACACTGTAGA TGCCATCTCA GAGAGTCTCC GGCAAGGTGG TGGCAAACTG AACTTTGATG 
     3241 AGCTTCGGCA AGACCTGAAA GGGAAGGGCC ATACAGATGC AGAGATTGAG GCCATATTCA 
     3301 CTAAATATGA CCAGGATGGC GACCAGGAAC TGACCGAGCG TGAGCATCAA CAGATGAGAG 
     3361 ATGACTTGGA GAAAGAGAGG GAGGACCTAG ACTTGGAACA CAGCTCTTTA CCACGTCCGA 
     3421 TGAGCAGCAG AAGTTTCCCC AGAAGCCTGG ATGACTCCGA GGAGGAGGAT GACGAAGACA 
     3481 GTGGCCATAG CTCCAGGAGG AGGGGAAGCA TCTCCAGTGG GGTTTCCTAT GAAGAGTTCC 
     3541 AAGTACTGGT GAGGCGCGTG GACCGCATGG AGCACTCCAT TGGCAGCATC GTTTCCAAGA 
     3601 TTGATGCCGT GATTGTCAAG CTGGAGATCA TGGAGCGGGC CAAGCTGAAG AGACGAGAGG 
     3661 TGTTAGGACG GCTGCTGGAT GGCGTGGCTG AGGATGCGCG ACTGGGTCGG GACAGTGAGA 
     3721 TCCACAGGGA GCAGATGGAG CGCCTGGTGC GGGAAGAGCT GGAGCGCTGG GAATCGGATG 
     3781 ATGCAGCTTC GCAAACAGGT CATGGTGTAA GCACACAAGT GGGACTCGGT GGCCAGCCCC 
     3841 ACCCCAGAAA CCCGCGCCCT CCTTCCTCCC AGTCTGCAGA GGGCCTGGAA GGTGGAGGTG 
     3901 GAAATGGAAG TGCCAACGTC CATGCCAGCG GACCGACGCG TACGCGGCCG CTCGAGCAGA 
     3961 AACTCATCTC AGAAGAGGAT CTGGCAGCAA ATGATATCCT GGATTACAAG GATGACGACG 
     4021 ATAAGGTTTA AACGGCCGGC CGCGGTCATA GCTGTTTCCT GAACAGATCC CGGGTGGCAT 
     4081 CCCTGTGACC CCTCCCCAGT GCCTCTCCTG GCCCTGGAAG TTGCCACTCC AGTGCCCACC 
     4141 AGCCTTGTCC TAATAAAATT AAGTTGCATC ATTTTGTCTG ACTAGGTGTC CTTCTATAAT 
     4201 ATTATGGGGT GGAGGGGGGT GGTATGGAGC AAGGGGCAAG TTGGGAAGAC AACCTGTAGG 
     4261 GCCTGCGGGG TCTATTGGGA ACCAAGCTGG AGTGCAGTGG CACAATCTTG GCTCACTGCA 
     4321 ATCTCCGCCT CCTGGGTTCA AGCGATTCTC CTGCCTCAGC CTCCCGAGTT GTTGGGATTC 
     4381 CAGGCATGCA TGACCAGGCT CAGCTAATTT TTGTTTTTTT GGTAGAGACG GGGTTTCACC 
     4441 ATATTGGCCA GGCTGGTCTC CAACTCCTAA TCTCAGGTGA TCTACCCACC TTGGCCTCCC 
     4501 AAATTGCTGG GATTACAGGC GTGAACCACT GCTCCCTTCC CTGTCCTTCT GATTTTAAAA 
     4561 TAACTATACC AGCAGGAGGA CGTCCAGACA CAGCATAGGC TACCTGGCCA TGCCCAACCG 
     4621 GTGGGACATT TGAGTTGCTT GCTTGGCACT GTCCTCTCAT GCGTTGGGTC CACTCAGTAG 
     4681 ATGCCTGTTG AATTGGGTAC GCGGCCAGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT 
     4741 AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA 
     4801 GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC 
     4861 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT 
     4921 ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT 
     4981 GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG 
     5041 CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA 
     5101 CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA 
     5161 CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC 
     5221 GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG 
     5281 AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG 
     5341 TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA 
     5401 GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC 
     5461 TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG 
     5521 GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA 
     5581 TGAGTAACCT GAGGCTATGG CAGGGCCTGC CGCCCCGACG TTGGCTGCGA GCCCTGGGCC 
     5641 TTCACCCGAA CTTGGGGGGT GGGGTGGGGA AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA 
     5701 ATGGGGTCTC GGTGGGGTAT CGACAGAGTG CCAGCCCTGG GACCGAACCC CGCGTTTATG 
     5761 AACAAACGAC CCAACACCGT GCGTTTTATT CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT 
     5821 TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA 
     5881 CTCCAGCATG AGATCCCCGC GCTGGAGGAT CATCCAGCCG GCGTCCCGGA AAACGATTCC 
     5941 GAAGCCCAAC CTTTCATAGA AGGCGGCGGT GGAATCGAAA TCTCGTGATG GCAGGTTGGG 
     6001 CGTCGCTTGG TCGGTCATTT CGAACCCCAG AGTCCCGCTC AGAAGAACTC GTCAAGAAGG 
     6061 CGATAGAAGG CGATGCGCTG CGAATCGGGA GCGGCGATAC CGTAAAGCAC GAGGAAGCGG 
     6121 TCAGCCCATT CGCCGCCAAG CTCTTCAGCA ATATCACGGG TAGCCAACGC TATGTCCTGA 
     6181 TAGCGATCCG CCACACCCAG CCGGCCACAG TCGATGAATC CAGAAAAGCG GCCATTTTCC 
     6241 ACCATGATAT TCGGCAAGCA GGCATCGCCA TGGGTCACGA CGAGATCCTC GCCGTCGGGC 
     6301 ATGCTCGCCT TGAGCCTGGC GAACAGTTCG GCTGGCGCGA GCCCCTGATG CTCTTCGTCC 
     6361 AGATCATCCT GATCGACAAG ACCGGCTTCC ATCCGAGTAC GTGCTCGCTC GATGCGATGT 
     6421 TTCGCTTGGT GGTCGAATGG GCAGGTAGCC GGATCAAGCG TATGCAGCCG CCGCATTGCA 
     6481 TCAGCCATGA TGGATACTTT CTCGGCAGGA GCAAGGTGAG ATGACAGGAG ATCCTGCCCC 
     6541 GGCACTTCGC CCAATAGCAG CCAGTCCCTT CCCGCTTCAG TGACAACGTC GAGCACAGCT 
     6601 GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC GATAGCCGCG CTGCCTCGTC TTGCAGTTCA 
     6661 TTCAGGGCAC CGGACAGGTC GGTCTTGACA AAAAGAACCG GGCGCCCCTG CGCTGACAGC 
     6721 CGGAACACGG CGGCATCAGA GCAGCCGATT GTCTGTTGTG CCCAGTCATA GCCGAATAGC 
     6781 CTCTCCACCC AAGCGGCCGG AGAACCTGCG TGCAATCCAT CTTGTTCAAT CATGCGAAAC 
     6841 GATCCTCATC CTGTCTCTTG ATCGATCTTT GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC 
     6901 TCACTACTTC TGGAATAGCT CAGAGGCCGA GGCGGCCTCG GCCTCTGCAT AAATAAAAAA 
     6961 AATTAGTCAG CCATGGGGCG GAGAATGGGC GGAACTGGGC GGAGTTAGGG GCGGGATGGG 
     7021 CGGAGTTAGG GGCGGGACTA TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     7081 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCTGGTTGCT GACTAATTGA GATGCATGCT 
     7141 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCCTAACT GACACACATT 
     7201 CCACAGCTGG TTCTTTCCGC CTCAGGACTC TTCCTTTTTC AATATTATTG AAGCATTTAT 
     7261 CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA 
     7321 GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG CGCCCTGTAG CGGCGCATTA 
     7381 AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG 
     7441 CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA 
     7501 GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC 
     7561 AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT 
     7621 CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA 
     7681 ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC 
     7741 TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTT
//