LOCUS       MR211333                7776 bp    DNA     circular  SYN 
DEFINITION  Vps54 (Myc-DDK-tagged) - Mouse vacuolar protein sorting 54 (yeast) (cDNA clone MGC:32446 IMAGE:5042879)
ACCESSION   BC025012
VERSION     BC025012.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3933..4024 
                     /label="Myc-DDK"  
     misc_feature    4054..4639 
                     /label="PolyA signal"  
     misc_feature    4111..4130 
                     /label="Restriction Enzyme"  
     misc_feature    4915..5587 
                     /label="ColE1"  
     misc_feature    6017..6811 
                     /label="Kan/Neo"  
     misc_feature    6846..7148 
                     /label="SV40 ori"  
     misc_feature    7234..7711 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3915..3921 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3915 
                     /label="Vps54"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCTTCAAGC CACAGTTCTT CTCCAGTGCC TCAAGGAAGC AGCAGTGATG 
     1081 TTTTCTTTAA AAAAGAGGTA GATCCAACAA AACACATTCG ACCTGTGCAG TCACTGCCAG 
     1141 ATGTGTGTCC CAAGGAACCC ACAGTTACAG ATCAACATAG ATGGACTGTG TATCATTCCA 
     1201 AAGTAAATCT CCCAGCAGCA TTAAATGATC CTACACTAGC AAAAAGAGAA TCTGACTTCT 
     1261 TCACAAAAAC TTGGGGATTG GACTTTGTGG ACACTGAAGT CATACCTTCA CTCTACCTCC 
     1321 CACAGATCAG CAAGGAGAAT TTTATAGCAT ATCAGCAGGA GATCTCTCAG AGAGAGAAGA 
     1381 TTCATGAGAG ATGCAAGAAT ATTTGTCCTC CTAAAGATAC CTTTGACAGG ACTCTTTTAC 
     1441 ATATTCATGA TAAATCCAGG ACAGATCTGG AGCAAGTACC TAAGATTTTT ATGAAACCAG 
     1501 ATTTTGCCTT GGATGATTCT TTAACTTTTA ATTCAGTTTT ACCATGGTCT CATTTTAATA 
     1561 CTGCTGGTGG GAAAGGAAGT CGCGACGCAG CTTCTTCCAA GTTGCTTCAG GAAAAGCTGA 
     1621 GCCATTATCT GGATATTGTG GAAGTAAACA TTGCTCACCA GATCTCTCTA CGTTCAGAAG 
     1681 CATTTTTTCA TGCAATGACC TCTCAACACG AATTGCAGGA CTACCTCAAG AAAACTACCC 
     1741 AGGCTGTAAA AATGCTTCGA GATAAAATTG CACAGATTGA TAAAGTTATG TGTGAAGGAT 
     1801 CACTACAGAT TTTAAGACTG GCACTTACCA GAAATAATTG TGTTAAAGTA TACAATAAAC 
     1861 TGAAGTTAAT GGCCACTGTA CACCAAACTC AGCCTACAGT GCAGGTGTTG TTATCTACTT 
     1921 CTGAATTTGT TGGAGCGTTG GACTTAATAG CAACAACTCA AGAGGTTCTG CAGCAGGAGC 
     1981 TTCAGGGCAT TCACAGCTTC CGGCATTTGG GATCACAGCT TTGTGAATTA GAAAAGCTGA 
     2041 TTGATAAAAT GATGATTGCA GAATTTTCTA CTTATTCTCA TAGTGACTTA AATAGACCAC 
     2101 TGGAAGGTGA ATGCCAAGTT TTAGAAGAGG AAAGATTAGT ATCTCTTGTA TTTGGGCTTT 
     2161 TGAAACAAAG AAAACTTAAT TTTTTAGAAA TCTATGGTGA AGAAATGATT ATTACAGCAA 
     2221 AGAACATCAT TAAAGAGCGT GTGATTAATA AAGTTTCACA AATAGAAGAA ATAGACACAG 
     2281 ACGTTGTTGT AAAGCTTGCA GATCAAATGA GAATGTTGAA TTTTCCCCAG TGGATTGATC 
     2341 TGTTAAAGGA CATTTTCTCT AAGTTTACAG TTTTCCTACA GAGAGTTAAG GCAACATTGA 
     2401 ATATTATCCA CAGTGTTGTT CTGTCAGTTC TTGAGAAAAG CCAAAGGACT AGAGAATTGG 
     2461 AAGAGATCCC ACAGCAGAGG AGCGCTGGGA AGGACAGCTC CTTGGACACA GACGTGGCTT 
     2521 ACCTGACACA CGAGGGCTGG TTCATAAGTG ATGCTTTCAG TGAGGGTGAG CCAGCATCTG 
     2581 CGGCGGTTGA TACTACCTCT CAAAGAAACA CGTCTCCACA TAGCGAGCCC TGCAGCAGTG 
     2641 ATTCGGTATC AGAACCAGAA TGTACTACTG ATTCTTCATC CAGCAAGGAG CAGACATCAG 
     2701 CGTGTGCTCC TCCAGGAGGT ATAGAGATTA TAGTCAGTGA AGACATGAGG TTAACTGACT 
     2761 TGGAGCTAGG AAAACTAGCA AGTAACATCC AGGAGTTACT GTGTAATGCT TCAGATGTAT 
     2821 GCCATGATCG AGCTGTCAAA TTTCTCATGT CAAGAGCAAA GGACGGTTTT CTTGAAAAAT 
     2881 TGAATTCCAC GGAATTCATA GCACTCTCGA GATTAATGGA GACATTCATA GTGGACACTG 
     2941 AACAGATCTG TGGGAGGAAG AGCACGTCAC TGCTTGGGGC CCTTCAGAGC CAAGCAAATA 
     3001 AGTTTGTGAA CAGGTTTCAT GAAGAGAGGC GGACCAAGCT CAGCCTCCTC TTAGACAACG 
     3061 AGCGTTGGAA GCAGGCTGAT GTTCCTGCAG AATTTCAGGA TCTTGTTGAT TCTATAGCGG 
     3121 ATGGAAAGAT TGCTTTACCT GAAAAAAAGC CAGTGGTTAC AGAAGAAAGG AAGCCAGCTG 
     3181 ATGTTCTTGT TGTTGAGGGA CACCAGTATG CAGTTGTCGG AACCGTCTTG CTGTTAATAA 
     3241 GAATCATTCT TGAATATTGC CAGTGTGTGG ATAACATCCC CTCCGTCACT ACTGACATGC 
     3301 TCACTCGCCT GACAGATCTG CTGAAGTACT TCAATTCAAG AAGTTGCCAG TTAGTTCTTG 
     3361 GAGCTGGTGC TTTGCAAGTT GTTGGACTAA AAACAATTAC TACAAAAAAC TTGGCCCTCT 
     3421 CTTCACGGTG TCTGCAGTTG ATTGTGCACT ACATTCCCGT GATCCGGGCT CACTTTGAAG 
     3481 CCCGACTGCC CCCCAAGCAA TGGAGCATGC TTAGACATTT TGATCACATC ACAAAGGATT 
     3541 ACCATGATCA TATAGCTGAA ATTTCAGCTA AGCTTGTAGC GATAATGGAT AGCCTATTTG 
     3601 ATAAGCTGTT ATCAAAGTAT GAGGTGAAAG CTCCTGTTCC TTCCCCGTGT TTCAGGAATA 
     3661 TTTGTAAGCA AATGACAAAA ATGCATGAAG CTATATTTGA TCTTCTTCCC GAAGAACAAA 
     3721 CACAGATGTT ATTTTTAAGA ATTAATGCAA GTTACAAACT CCACTTGAAA AAACAGTTAT 
     3781 CACACTTAAA TGTAATAAAT GACGGAGGAC CTCAAAATGG GTTGGTCACA GCAGATGTAG 
     3841 CTTTTTACAC TGGAAATCTT CAAGCCTTAA AAGGCCTTAA AGATCTGGAT CTAAATATGG 
     3901 CCGAGATCTG GGAACAGAAG AGGACGCGTA CGCGGCCGCT CGAGCAGAAA CTCATCTCAG 
     3961 AAGAGGATCT GGCAGCAAAT GATATCCTGG ATTACAAGGA TGACGACGAT AAGGTTTAAA 
     4021 CGGCCGGCCG CGGTCATAGC TGTTTCCTGA ACAGATCCCG GGTGGCATCC CTGTGACCCC 
     4081 TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT GCCACTCCAG TGCCCACCAG CCTTGTCCTA 
     4141 ATAAAATTAA GTTGCATCAT TTTGTCTGAC TAGGTGTCCT TCTATAATAT TATGGGGTGG 
     4201 AGGGGGGTGG TATGGAGCAA GGGGCAAGTT GGGAAGACAA CCTGTAGGGC CTGCGGGGTC 
     4261 TATTGGGAAC CAAGCTGGAG TGCAGTGGCA CAATCTTGGC TCACTGCAAT CTCCGCCTCC 
     4321 TGGGTTCAAG CGATTCTCCT GCCTCAGCCT CCCGAGTTGT TGGGATTCCA GGCATGCATG 
     4381 ACCAGGCTCA GCTAATTTTT GTTTTTTTGG TAGAGACGGG GTTTCACCAT ATTGGCCAGG 
     4441 CTGGTCTCCA ACTCCTAATC TCAGGTGATC TACCCACCTT GGCCTCCCAA ATTGCTGGGA 
     4501 TTACAGGCGT GAACCACTGC TCCCTTCCCT GTCCTTCTGA TTTTAAAATA ACTATACCAG 
     4561 CAGGAGGACG TCCAGACACA GCATAGGCTA CCTGGCCATG CCCAACCGGT GGGACATTTG 
     4621 AGTTGCTTGC TTGGCACTGT CCTCTCATGC GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA 
     4681 TTGGGTACGC GGCCAGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC 
     4741 CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG 
     4801 GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA 
     4861 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA 
     4921 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG 
     4981 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG 
     5041 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT 
     5101 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA 
     5161 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG 
     5221 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT 
     5281 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC 
     5341 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG 
     5401 CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG 
     5461 GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA 
     5521 GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAACCTGA 
     5581 GGCTATGGCA GGGCCTGCCG CCCCGACGTT GGCTGCGAGC CCTGGGCCTT CACCCGAACT 
     5641 TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG 
     5701 TGGGGTATCG ACAGAGTGCC AGCCCTGGGA CCGAACCCCG CGTTTATGAA CAAACGACCC 
     5761 AACACCGTGC GTTTTATTCT GTCTTTTTAT TGCCGTCATA GCGCGGGTTC CTTCCGGTAT 
     5821 TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC CCTAGGGTGG GCGAAGAACT CCAGCATGAG 
     5881 ATCCCCGCGC TGGAGGATCA TCCAGCCGGC GTCCCGGAAA ACGATTCCGA AGCCCAACCT 
     5941 TTCATAGAAG GCGGCGGTGG AATCGAAATC TCGTGATGGC AGGTTGGGCG TCGCTTGGTC 
     6001 GGTCATTTCG AACCCCAGAG TCCCGCTCAG AAGAACTCGT CAAGAAGGCG ATAGAAGGCG 
     6061 ATGCGCTGCG AATCGGGAGC GGCGATACCG TAAAGCACGA GGAAGCGGTC AGCCCATTCG 
     6121 CCGCCAAGCT CTTCAGCAAT ATCACGGGTA GCCAACGCTA TGTCCTGATA GCGATCCGCC 
     6181 ACACCCAGCC GGCCACAGTC GATGAATCCA GAAAAGCGGC CATTTTCCAC CATGATATTC 
     6241 GGCAAGCAGG CATCGCCATG GGTCACGACG AGATCCTCGC CGTCGGGCAT GCTCGCCTTG 
     6301 AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC CCCTGATGCT CTTCGTCCAG ATCATCCTGA 
     6361 TCGACAAGAC CGGCTTCCAT CCGAGTACGT GCTCGCTCGA TGCGATGTTT CGCTTGGTGG 
     6421 TCGAATGGGC AGGTAGCCGG ATCAAGCGTA TGCAGCCGCC GCATTGCATC AGCCATGATG 
     6481 GATACTTTCT CGGCAGGAGC AAGGTGAGAT GACAGGAGAT CCTGCCCCGG CACTTCGCCC 
     6541 AATAGCAGCC AGTCCCTTCC CGCTTCAGTG ACAACGTCGA GCACAGCTGC GCAAGGAACG 
     6601 CCCGTCGTGG CCAGCCACGA TAGCCGCGCT GCCTCGTCTT GCAGTTCATT CAGGGCACCG 
     6661 GACAGGTCGG TCTTGACAAA AAGAACCGGG CGCCCCTGCG CTGACAGCCG GAACACGGCG 
     6721 GCATCAGAGC AGCCGATTGT CTGTTGTGCC CAGTCATAGC CGAATAGCCT CTCCACCCAA 
     6781 GCGGCCGGAG AACCTGCGTG CAATCCATCT TGTTCAATCA TGCGAAACGA TCCTCATCCT 
     6841 GTCTCTTGAT CGATCTTTGC AAAAGCCTAG GCCTCCAAAA AAGCCTCCTC ACTACTTCTG 
     6901 GAATAGCTCA GAGGCCGAGG CGGCCTCGGC CTCTGCATAA ATAAAAAAAA TTAGTCAGCC 
     6961 ATGGGGCGGA GAATGGGCGG AACTGGGCGG AGTTAGGGGC GGGATGGGCG GAGTTAGGGG 
     7021 CGGGACTATG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     7081 GCCTGGGGAC TTTCCACACC TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     7141 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCCTAACTGA CACACATTCC ACAGCTGGTT 
     7201 CTTTCCGCCT CAGGACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT 
     7261 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC 
     7321 ACATTTCCCC GAAAAGTGCC ACCTGACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT 
     7381 GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC 
     7441 GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG 
     7501 GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT 
     7561 TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG 
     7621 TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT 
     7681 ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA 
     7741 AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTT
//