LOCUS       MR211332                7773 bp    DNA     circular  SYN 
DEFINITION  Rtn3 (Myc-DDK-tagged) - Mouse reticulon 3 (Rtn3), transcript variant 1
ACCESSION   NM_001003934
VERSION     NM_001003934.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3930..4021 
                     /label="Myc-DDK"  
     misc_feature    4051..4636 
                     /label="PolyA signal"  
     misc_feature    4108..4127 
                     /label="Restriction Enzyme"  
     misc_feature    4912..5584 
                     /label="ColE1"  
     misc_feature    6014..6808 
                     /label="Kan/Neo"  
     misc_feature    6843..7145 
                     /label="SV40 ori"  
     misc_feature    7231..7708 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3912..3918 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3912 
                     /label="Rtn3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGGAGTCG TCAGCGGCCA CTCAGTCCCC GTCAGTCTCC TCGTCGTCCT 
     1081 CCGGGGCCGA GCCGTCAGCT CTCGGCGGCG GCGGCGGGAG CCCTGGAGCC TGCCCCGCCC 
     1141 TGGGGGCGAA GAGCTGCGGC TCCTCGTGTG CGGATTCCTT TGTTTCTTCC TCTTCCTCTC 
     1201 AGCCTGTATC TATATTTTCG ACCTCACAAG CGGGATTGAG CTCTCTTTGC TCTGATGAGC 
     1261 CACCTTCAAA AAGTATGACT TCCTCCTTTC TTTCATCTTC TGAAATACAT AACCCTGACC 
     1321 CTACAACACC GCTTGGAGAA AAGAGTGAAA CATTAGGTAG CCAGTTTGTT CTAGCTAAAG 
     1381 GAAAAGACCC CTTGGTTCTT CTAGATAAGA AAAAACTGGA CTCACCTCAG GGGACCAACA 
     1441 AGGACAGAGT AGACGCTCCG GTTTCTCTTG CAACTGGCAT TCCTTGCAGC CACCCTTCTA 
     1501 TTCCAGACAG TTTCCCAGAG CAACCTGCTT TTCTGTCAAA AGAAATTGGT CCAGCAGAAG 
     1561 AGTGGGTAGT TAAAGACCAA GAACCCAAGA ACCCAAACAA GGTTCCAGAT GGGGAGGACA 
     1621 GAAGTGCACT GGATTTTGGG CAGTCTAAGG CAGAACACAT TTGTACATAT TCCTTGTCCC 
     1681 CATCTGAACT TCCAGTAGCC AGTGTAGAAA AAGATTCTCC TGAGTCACCG TTTGAAGTAA 
     1741 TTATTGACAA AGCAACATTT GACAGAGAAT TTAAAGATTT GTATAAGGAA AACCCAAATG 
     1801 ATTTGGGTGG CTGGGCAGCT CATGGCGATA GAGAATCCCC CGCAGACTTG TTGGAAATGA 
     1861 ATGACAAACT CTTTCCACTG AGAAATAAAG AGGCAGGGCG TTACCCATCC TCTGTACTGC 
     1921 TCGGTAGACA GTTCTCACAC ACTACAGCAG CACTGGAAGA GGTGTCTAGA TGTGTGAATG 
     1981 ATATGCATAA CTTTACTAAT GAAATACTGA CTTGGGACTT AGATCCCCAA GCAAAACAAC 
     2041 AGGCCAATAA AACATCTTGC ACCACAGAAA GTACAGGACT AGACAGGAGT GAACTTCGCT 
     2101 CAGAAATTCC AGTTATAAAT CTTAAAACAA ACCCTCAACA GAAAATGCCT GTATGTTCTT 
     2161 TTAATGGGAG CACTCCCATT ACCAAATCCA CAGGTGACTG GACAGAAGCA TTTACAGAAG 
     2221 GAAAACCTGT AAGAGACTAC CTCAGTTCCA CAAAAGAAGC TGGTGGCAAC GGTGTGCCAG 
     2281 GCAGTTCTCA GCTTCATTCT GAGCTGCCTG GCTCTATGCC TGAGAAATGG GTCTCAGGCT 
     2341 CTGGAGCAGC CACAGTGGAA GTAACTTTAC CTAACCTGAG GGGTGCCTGG CCTAACTCTG 
     2401 TGATGGGGGA AGTCACAGAG GTTGATAGTT CTGGGGAATC TGATGACACA GTAATAGAGG 
     2461 ACATCACAGA AAAACCTGAC TCCCTTCCAA GTGCTGCTGC AAAAACAAGT GAAAGGGAAA 
     2521 TCAAAGAGAC TCCCAGTCGT GAAACTGTGA GGAGTGAAAT GTGTGAAAAC TCTGAGCAGC 
     2581 CGCAGGCCCA GCCAGAAACT CCTACTCAGA AGAGTCTGGA AGGTGAGGTG GCTTCACAAG 
     2641 TACCTAATAC CCTGAATGAA GTCACACCTG AAAAGCTTGA TATGACTAAC AACCCCAAAG 
     2701 TTTGCTCAGC AGCACCTCCA AGTGTTCTTA ATGAGACAGG ATTCTCACTA ACTGTGCCAG 
     2761 CTTCTGCCAA GTTGGAATCT TTGCTTGGAA AATATGTTGA AGATACAGAT GGTTCCTCCC 
     2821 CAGAGGACTT GATGGCTGTC CTCACAGGAG CCGAGGAGAA GGGGATAGTG GATAAAGAGG 
     2881 AAGGTGATGT TTTGGAAGCA GTGTTAGAGA AGATAGCAGA CTTTAAAAAC ACTTTGCCTG 
     2941 TGGAACTCTT GCATGAAAGT GAGCTAAGTG GTTCTGAAAC CAAAAATATT AAAAGCAAAT 
     3001 ACAGTGAAGA CAGCAGAGAA ACAACTGGAG GTGCCCCTAC GATGTCTCCC GACTTAGAGC 
     3061 AGGAGCAGCT CACCATCAGA GCCATTAAAG AATTAGGAGA AAGGCAAGCT GAGAAGGTGC 
     3121 AGGATGAAGG AATATCTTCT GGAGGAAAAC TCAAGCAAAC CTTTGCTCCA CAGTCTGGGC 
     3181 CACAGAGTTC ATCTGACATC CTAGAACACA CAGATGTCAA AACTGGATCT GATCTTGGAA 
     3241 TTCCCAAAAA TCCTACTATC ATCAAAAACA CTAGAATAGA TTCTATTTCC AGCCTTACCA 
     3301 AGACTGAAAT GGTTAACAAG AATGTTCTAG CAAGACTTCT CAGTGATTTC CCAGTGCACG 
     3361 ATCTGATTTT CTGGCGAGAT GTGAAGAAGA CTGGGTTTGT CTTTGGCACC ACACTGATCA 
     3421 TGCTGCTCTC TCTGGCAGCC TTCAGTGTTA TCAGTGTGGT CTCTTACCTC ATCCTGGCTC 
     3481 TTCTCTCTGT CACCATCAGC TTCAGAGTCT ATAAGTCTGT CATTCAAGCT GTGCAGAAGT 
     3541 CAGAAGAAGG ACATCCATTC AAGGCCTACT TGGATGTGGA CATTACCCTG TCTTCAGAAG 
     3601 CTTTCCACAA CTACATGAAT GCTGCGATGG TGCATGTCAA CAAGGCCCTC AAACTCATTA 
     3661 TTCGTCTCTT TCTGGTAGAA GACTTGGTTG ACTCCTTGAA GCTGGCTGTC TTCATGTGGC 
     3721 TGATGACCTA TGTTGGTGCT GTTTTTAATG GAATTACCCT TCTGATTCTC GCCGAGCTGC 
     3781 TTGTCTTCAG CGTCCCAATT GTCTATGAGA AGTATAAGAC ACAGATTGAC CACTATGTTG 
     3841 GGATTGCCCG GGATCAGACC AAGTCAATTG TTGAAAAGAT CCAAGCAAAG CTTCCTGGAA 
     3901 TCGCCAAAAA AAAGGCAGAA ACGCGTACGC GGCCGCTCGA GCAGAAACTC ATCTCAGAAG 
     3961 AGGATCTGGC AGCAAATGAT ATCCTGGATT ACAAGGATGA CGACGATAAG GTTTAAACGG 
     4021 CCGGCCGCGG TCATAGCTGT TTCCTGAACA GATCCCGGGT GGCATCCCTG TGACCCCTCC 
     4081 CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA 
     4141 AAATTAAGTT GCATCATTTT GTCTGACTAG GTGTCCTTCT ATAATATTAT GGGGTGGAGG 
     4201 GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG AAGACAACCT GTAGGGCCTG CGGGGTCTAT 
     4261 TGGGAACCAA GCTGGAGTGC AGTGGCACAA TCTTGGCTCA CTGCAATCTC CGCCTCCTGG 
     4321 GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC GAGTTGTTGG GATTCCAGGC ATGCATGACC 
     4381 AGGCTCAGCT AATTTTTGTT TTTTTGGTAG AGACGGGGTT TCACCATATT GGCCAGGCTG 
     4441 GTCTCCAACT CCTAATCTCA GGTGATCTAC CCACCTTGGC CTCCCAAATT GCTGGGATTA 
     4501 CAGGCGTGAA CCACTGCTCC CTTCCCTGTC CTTCTGATTT TAAAATAACT ATACCAGCAG 
     4561 GAGGACGTCC AGACACAGCA TAGGCTACCT GGCCATGCCC AACCGGTGGG ACATTTGAGT 
     4621 TGCTTGCTTG GCACTGTCCT CTCATGCGTT GGGTCCACTC AGTAGATGCC TGTTGAATTG 
     4681 GGTACGCGGC CAGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC 
     4741 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA 
     4801 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA 
     4861 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC 
     4921 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA 
     4981 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA 
     5041 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA 
     5101 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA 
     5161 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG 
     5221 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG 
     5281 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 
     5341 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG 
     5401 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA 
     5461 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT 
     5521 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AACCTGAGGC 
     5581 TATGGCAGGG CCTGCCGCCC CGACGTTGGC TGCGAGCCCT GGGCCTTCAC CCGAACTTGG 
     5641 GGGGTGGGGT GGGGAAAAGG AAGAAACGCG GGCGTATTGG CCCCAATGGG GTCTCGGTGG 
     5701 GGTATCGACA GAGTGCCAGC CCTGGGACCG AACCCCGCGT TTATGAACAA ACGACCCAAC 
     5761 ACCGTGCGTT TTATTCTGTC TTTTTATTGC CGTCATAGCG CGGGTTCCTT CCGGTATTGT 
     5821 CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT AGGGTGGGCG AAGAACTCCA GCATGAGATC 
     5881 CCCGCGCTGG AGGATCATCC AGCCGGCGTC CCGGAAAACG ATTCCGAAGC CCAACCTTTC 
     5941 ATAGAAGGCG GCGGTGGAAT CGAAATCTCG TGATGGCAGG TTGGGCGTCG CTTGGTCGGT 
     6001 CATTTCGAAC CCCAGAGTCC CGCTCAGAAG AACTCGTCAA GAAGGCGATA GAAGGCGATG 
     6061 CGCTGCGAAT CGGGAGCGGC GATACCGTAA AGCACGAGGA AGCGGTCAGC CCATTCGCCG 
     6121 CCAAGCTCTT CAGCAATATC ACGGGTAGCC AACGCTATGT CCTGATAGCG ATCCGCCACA 
     6181 CCCAGCCGGC CACAGTCGAT GAATCCAGAA AAGCGGCCAT TTTCCACCAT GATATTCGGC 
     6241 AAGCAGGCAT CGCCATGGGT CACGACGAGA TCCTCGCCGT CGGGCATGCT CGCCTTGAGC 
     6301 CTGGCGAACA GTTCGGCTGG CGCGAGCCCC TGATGCTCTT CGTCCAGATC ATCCTGATCG 
     6361 ACAAGACCGG CTTCCATCCG AGTACGTGCT CGCTCGATGC GATGTTTCGC TTGGTGGTCG 
     6421 AATGGGCAGG TAGCCGGATC AAGCGTATGC AGCCGCCGCA TTGCATCAGC CATGATGGAT 
     6481 ACTTTCTCGG CAGGAGCAAG GTGAGATGAC AGGAGATCCT GCCCCGGCAC TTCGCCCAAT 
     6541 AGCAGCCAGT CCCTTCCCGC TTCAGTGACA ACGTCGAGCA CAGCTGCGCA AGGAACGCCC 
     6601 GTCGTGGCCA GCCACGATAG CCGCGCTGCC TCGTCTTGCA GTTCATTCAG GGCACCGGAC 
     6661 AGGTCGGTCT TGACAAAAAG AACCGGGCGC CCCTGCGCTG ACAGCCGGAA CACGGCGGCA 
     6721 TCAGAGCAGC CGATTGTCTG TTGTGCCCAG TCATAGCCGA ATAGCCTCTC CACCCAAGCG 
     6781 GCCGGAGAAC CTGCGTGCAA TCCATCTTGT TCAATCATGC GAAACGATCC TCATCCTGTC 
     6841 TCTTGATCGA TCTTTGCAAA AGCCTAGGCC TCCAAAAAAG CCTCCTCACT ACTTCTGGAA 
     6901 TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC TGCATAAATA AAAAAAATTA GTCAGCCATG 
     6961 GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG 
     7021 GACTATGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     7081 TGGGGACTTT CCACACCTGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     7141 TGCTGGGGAG CCTGGGGACT TTCCACACCC TAACTGACAC ACATTCCACA GCTGGTTCTT 
     7201 TCCGCCTCAG GACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC 
     7261 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA 
     7321 TTTCCCCGAA AAGTGCCACC TGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG 
     7381 GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT 
     7441 TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG 
     7501 CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG 
     7561 GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG 
     7621 GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC 
     7681 TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT 
     7741 GAGCTGATTT AACAAAAATT TAACGCGAAT TTT
//