LOCUS       MR211329                7770 bp    DNA     circular  SYN 
DEFINITION  Kif5b (Myc-DDK-tagged) - Mouse kinesin family member 5B (Kif5b)
ACCESSION   NM_008448
VERSION     NM_008448.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3927..4018 
                     /label="Myc-DDK"  
     misc_feature    4048..4633 
                     /label="PolyA signal"  
     misc_feature    4105..4124 
                     /label="Restriction Enzyme"  
     misc_feature    4909..5581 
                     /label="ColE1"  
     misc_feature    6011..6805 
                     /label="Kan/Neo"  
     misc_feature    6840..7142 
                     /label="SV40 ori"  
     misc_feature    7228..7705 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3909..3915 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3909 
                     /label="Kif5b"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGGACCCG GCGGAGTGCA ACATCAAAGT GATGTGTCGC TTCAGACCTC 
     1081 TCAACGAATC TGAAGTGAAC CGCGGCGATA AGTACGTCGC CAAATTCCAG GGAGAAGACA 
     1141 CGGTGATGAT CGCGTCCAAG CCTTATGCCT TTGATCGTGT GTTCCAGTCA AGCACATCTC 
     1201 AAGAGCAAGT ATACAACGAC TGTGCAAAAA AGATTGTTAA AGATGTTCTT GAGGGCTATA 
     1261 ATGGAACAAT ATTTGCATAT GGACAAACAT CATCTGGGAA GACCCACACG ATGGAGGGTA 
     1321 AACTTCATGA TCCAGAAGGC ATGGGAATTA TTCCAAGAAT AGTGCAAGAT ATTTTTAATT 
     1381 ATATTTACTC CATGGATGAA AATTTGGAAT TTCATATTAA GGTTTCATAT TTTGAAATAT 
     1441 ATTTGGATAA GATAAGGGAC TTGTTAGATG TTTCAAAGAC TAACCTTTCA GTCCATGAAG 
     1501 ACAAAAACCG TGTTCCCTAT GTAAAGGGGT GCACAGAACG TTTCGTGTGT AGTCCAGATG 
     1561 AAGTCATGGA TACCATAGAT GAAGGGAAAT CCAACAGACA TGTCGCAGTT ACAAATATGA 
     1621 ATGAACATAG CTCTAGGAGT CACAGCATAT TTCTTATTAA TGTAAAACAA GAGAATACAC 
     1681 AAACGGAACA GAAACTCAGT GGAAAGCTTT ATCTGGTTGA TTTAGCTGGC AGTGAGAAGG 
     1741 TTAGTAAGAC TGGGGCTGAA GGTGCTGTGC TGGATGAAGC TAAGAACATC AACAAGTCAC 
     1801 TTTCTGCACT TGGAAATGTC ATTTCTGCTT TGGCAGAGGG CAGTACCTAT GTTCCTTATC 
     1861 GAGATAGTAA AATGACCAGA ATTCTTCAAG ATTCATTAGG TGGCAACTGT AGGACCACTA 
     1921 TTGTCATATG CTGCTCTCCA TCATCATACA ATGAGTCTGA GACAAAGTCA ACACTCCTCT 
     1981 TTGGTCAAAG GGCCAAAACA ATTAAGAACA CAGTCTGTGT CAATGTAGAG TTAACTGCGG 
     2041 AGCAGTGGAA AAAGAAGTAT GAAAAAGAAA AGGAAAAAAA TAAAACTCTA CGGAACACTA 
     2101 TTCAGTGGCT GGAAAACGAG CTAAACCGTT GGCGTAACGG GGAGACAGTG CCTATTGATG 
     2161 AGCAGTTTGA CAAAGAGAAA GCTAATTTGG AAGCCTTCAC AGCGGATAAA GATATTGCTA 
     2221 TTACCAGTGA TAAACCAGCT GCTGCAGTCG GAATGGCTGG TAGTTTTACC GATGCTGAAA 
     2281 GAAGAAAGTG TGAAGAAGAA CTTGCTAAAT TGTATAAACA ACTTGATGAC AAGGATGAAG 
     2341 AGATTAACCA ACAAAGCCAA TTGGTAGAGA AATTGAAGAC ACAAATGCTG GATCAGGAAG 
     2401 AGCTTCTGGC ATCAACCAGA AGGGATCAAG ATAATATGCA AGCTGAACTG AATCGCCTCC 
     2461 AAGCAGAAAA TGATGCTTCT AAAGAAGAAG TCAAAGAAGT TTTACAGGCC TTAGAGGAAC 
     2521 TGGCTGTTAA TTATGATCAG AAGTCTCAGG AAGTTGAAGA CAAAACAAAG GAATATGAAT 
     2581 TGCTTAGTGA TGAATTGAAT CAAAAATCTG CAACTTTAGC AAGTATTGAT GCTGAGCTTC 
     2641 AGAAGCTGAA GGAAATGACC AACCACCAGA AGAAACGAGC AGCTGAAATG ATGGCATCAT 
     2701 TATTAAAAGA CCTTGCAGAA ATAGGAATTG CTGTGGGGAA TAACGATGTG AAGCAACCAG 
     2761 AAGGAACTGG TATGATAGAT GAAGAGTTTA CTGTTGCAAG ACTCTACATT AGCAAAATGA 
     2821 AATCAGAAGT AAAGACCATG GTGAAACGCT GCAAACAGCT AGAAAGCACA CAGACTGAGA 
     2881 GCAACAAAAA AATGGAAGAA AATGAGAAAG AGTTAGCAGC ATGCCAGCTT CGGATCTCCC 
     2941 AACATGAAGC CAAAATCAAG TCACTGACTG AGTACCTTCA GAATGTAGAA CAAAAGAAGA 
     3001 GGCAGCTGGA GGAGTCTGTT GATTCCCTTG GTGAGGAGCT AGTCCAACTC CGAGCACAAG 
     3061 AGAAAGTCCA TGAAATGGAA AAAGAGCACT TGAACAAGGT TCAGACTGCA AATGAAGTCA 
     3121 AGCAAGCTGT TGAGCAGCAG ATCCAGAGTC ACAGAGAAAC CCACCAAAAA CAAATCAGTA 
     3181 GTTTGCGAGA TGAAGTGGAG GCAAAGGAAA AGCTAATCAC TGACCTCCAA GACCAAAACC 
     3241 AGAAGATGGT GTTGGAGCAG GAACGGCTAA GGGTGGAGCA TGAGAGGCTG AAGGCTACAG 
     3301 ACCAAGAGAA GAGCAGGAAG CTGCACGAGC TCACGGTTAT GCAAGACAGA CGAGAACAAG 
     3361 CAAGACAAGA CTTGAAGGGT TTGGAGGAGA CCGTGGCAAA AGAACTTCAG ACTTTACACA 
     3421 ACCTGCGTAA GCTCTTTGTT CAGGACTTGG CTACCAGGGT GAAAAAGAGC GCCGAGGTCG 
     3481 ACTCTGACGA CACTGGCGGC AGTGCTGCAC AGAAGCAGAA AATCTCCTTC CTTGAAAACA 
     3541 ACCTTGAACA GCTCACCAAA GTGCACAAGC AGTTGGTACG CGATAATGCA GATCTTCGCT 
     3601 GTGAGCTTCC TAAGTTAGAG AAACGGCTTA GAGCTACTGC AGAAAGAGTG AAAGCTTTGG 
     3661 AGTCAGCCCT GAAAGAAGCC AAAGAAAATG CATCTCGAGA CCGTAAACGC TATCAGCAAG 
     3721 AAGTAGACCG GATAAAGGAA GCAGTCAGGT CAAAGAACAT GGCCAGAAGG GGACATTCTG 
     3781 CCCAGATTGC AAAGCCGATC CGTCCTGGAC AGCATCCAGC GGCCTCGCCA ACTCACCCGG 
     3841 GCACAGTTCG TGGAGGAGGC TCATTTGTTC AGAACAACCA GCCAGTGGGG CTTCGTGGTG 
     3901 GTGGAGGCAA GCAGTCGACG CGTACGCGGC CGCTCGAGCA GAAACTCATC TCAGAAGAGG 
     3961 ATCTGGCAGC AAATGATATC CTGGATTACA AGGATGACGA CGATAAGGTT TAAACGGCCG 
     4021 GCCGCGGTCA TAGCTGTTTC CTGAACAGAT CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA 
     4081 GTGCCTCTCC TGGCCCTGGA AGTTGCCACT CCAGTGCCCA CCAGCCTTGT CCTAATAAAA 
     4141 TTAAGTTGCA TCATTTTGTC TGACTAGGTG TCCTTCTATA ATATTATGGG GTGGAGGGGG 
     4201 GTGGTATGGA GCAAGGGGCA AGTTGGGAAG ACAACCTGTA GGGCCTGCGG GGTCTATTGG 
     4261 GAACCAAGCT GGAGTGCAGT GGCACAATCT TGGCTCACTG CAATCTCCGC CTCCTGGGTT 
     4321 CAAGCGATTC TCCTGCCTCA GCCTCCCGAG TTGTTGGGAT TCCAGGCATG CATGACCAGG 
     4381 CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA CGGGGTTTCA CCATATTGGC CAGGCTGGTC 
     4441 TCCAACTCCT AATCTCAGGT GATCTACCCA CCTTGGCCTC CCAAATTGCT GGGATTACAG 
     4501 GCGTGAACCA CTGCTCCCTT CCCTGTCCTT CTGATTTTAA AATAACTATA CCAGCAGGAG 
     4561 GACGTCCAGA CACAGCATAG GCTACCTGGC CATGCCCAAC CGGTGGGACA TTTGAGTTGC 
     4621 TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG TCCACTCAGT AGATGCCTGT TGAATTGGGT 
     4681 ACGCGGCCAG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA 
     4741 ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG 
     4801 TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA 
     4861 AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT 
     4921 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT 
     4981 GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT 
     5041 CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC 
     5101 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT 
     5161 ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC 
     5221 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT 
     5281 CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA 
     5341 ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA 
     5401 AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA 
     5461 AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT 
     5521 TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAC CTGAGGCTAT 
     5581 GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC GAGCCCTGGG CCTTCACCCG AACTTGGGGG 
     5641 GTGGGGTGGG GAAAAGGAAG AAACGCGGGC GTATTGGCCC CAATGGGGTC TCGGTGGGGT 
     5701 ATCGACAGAG TGCCAGCCCT GGGACCGAAC CCCGCGTTTA TGAACAAACG ACCCAACACC 
     5761 GTGCGTTTTA TTCTGTCTTT TTATTGCCGT CATAGCGCGG GTTCCTTCCG GTATTGTCTC 
     5821 CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG GTGGGCGAAG AACTCCAGCA TGAGATCCCC 
     5881 GCGCTGGAGG ATCATCCAGC CGGCGTCCCG GAAAACGATT CCGAAGCCCA ACCTTTCATA 
     5941 GAAGGCGGCG GTGGAATCGA AATCTCGTGA TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT 
     6001 TTCGAACCCC AGAGTCCCGC TCAGAAGAAC TCGTCAAGAA GGCGATAGAA GGCGATGCGC 
     6061 TGCGAATCGG GAGCGGCGAT ACCGTAAAGC ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA 
     6121 AGCTCTTCAG CAATATCACG GGTAGCCAAC GCTATGTCCT GATAGCGATC CGCCACACCC 
     6181 AGCCGGCCAC AGTCGATGAA TCCAGAAAAG CGGCCATTTT CCACCATGAT ATTCGGCAAG 
     6241 CAGGCATCGC CATGGGTCAC GACGAGATCC TCGCCGTCGG GCATGCTCGC CTTGAGCCTG 
     6301 GCGAACAGTT CGGCTGGCGC GAGCCCCTGA TGCTCTTCGT CCAGATCATC CTGATCGACA 
     6361 AGACCGGCTT CCATCCGAGT ACGTGCTCGC TCGATGCGAT GTTTCGCTTG GTGGTCGAAT 
     6421 GGGCAGGTAG CCGGATCAAG CGTATGCAGC CGCCGCATTG CATCAGCCAT GATGGATACT 
     6481 TTCTCGGCAG GAGCAAGGTG AGATGACAGG AGATCCTGCC CCGGCACTTC GCCCAATAGC 
     6541 AGCCAGTCCC TTCCCGCTTC AGTGACAACG TCGAGCACAG CTGCGCAAGG AACGCCCGTC 
     6601 GTGGCCAGCC ACGATAGCCG CGCTGCCTCG TCTTGCAGTT CATTCAGGGC ACCGGACAGG 
     6661 TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC TGCGCTGACA GCCGGAACAC GGCGGCATCA 
     6721 GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA TAGCCGAATA GCCTCTCCAC CCAAGCGGCC 
     6781 GGAGAACCTG CGTGCAATCC ATCTTGTTCA ATCATGCGAA ACGATCCTCA TCCTGTCTCT 
     6841 TGATCGATCT TTGCAAAAGC CTAGGCCTCC AAAAAAGCCT CCTCACTACT TCTGGAATAG 
     6901 CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC ATAAATAAAA AAAATTAGTC AGCCATGGGG 
     6961 CGGAGAATGG GCGGAACTGG GCGGAGTTAG GGGCGGGATG GGCGGAGTTA GGGGCGGGAC 
     7021 TATGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     7081 GGACTTTCCA CACCTGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     7141 TGGGGAGCCT GGGGACTTTC CACACCCTAA CTGACACACA TTCCACAGCT GGTTCTTTCC 
     7201 GCCTCAGGAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG 
     7261 AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT 
     7321 CCCCGAAAAG TGCCACCTGA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG 
     7381 GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC 
     7441 TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC 
     7501 CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT 
     7561 GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG 
     7621 TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG 
     7681 GTCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG 
     7741 CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//