LOCUS       MR211315                7761 bp    DNA     circular  SYN 
DEFINITION  Fgd1 (Myc-DDK-tagged) - Mouse FYVE, RhoGEF and PH domain containing 1 (Fgd1)
ACCESSION   NM_008001
VERSION     NM_008001.4
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3918..4009 
                     /label="Myc-DDK"  
     misc_feature    4039..4624 
                     /label="PolyA signal"  
     misc_feature    4096..4115 
                     /label="Restriction Enzyme"  
     misc_feature    4900..5572 
                     /label="ColE1"  
     misc_feature    6002..6796 
                     /label="Kan/Neo"  
     misc_feature    6831..7133 
                     /label="SV40 ori"  
     misc_feature    7219..7696 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3900..3906 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3900 
                     /label="Fgd1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCATGGCCAC CGAGTCCCGG GGGGCCCTGG GCCTTCGGAT CCCGAACGCT 
     1081 CAGCGGCAAA TACCCCCGGT GCCGCTCCGC TGGCCTGTGC CGACTCGGAC CCTGGAGCCT 
     1141 TGGAACCCGG GCTGCCGGTG AGCAGGGGCT CAGGCACGGC TCTTGGAGGC CCTTTGGATC 
     1201 CTCAGTTTGT TGGACCCTCG GATGCCAGCC TGGGAGCGCC TCCAAGCTCC CGGGTCTTAC 
     1261 CCTGCGGCCC TAGTCCTCAG CACCACCGGG CCCTGCGCTT CTCCTACCAC CTGGAGGGCT 
     1321 CGCAGCCTCG GCCTGGACTG CACCAGGGAA ACCGGATCCT GGTTAAAAGT TTGTCCCTTG 
     1381 ACCCTGGCCA GAGCCTTGAG CCTCATCCAG AAGGTCCCCA ACGGCTTCGC TCAGATCCAG 
     1441 GCCCTCCCAC TGAAATCCCT GGCCCACGTC CTTCACCACT GAAGCGGGCA CCGGGCCCAA 
     1501 AGCCACAGGT TCCCCCAAAG CCCAGCTACC TGCAGATGCC CCGGGTGCTT CCTCCTCCTG 
     1561 AGCCCATTCC TCCACCACCA TCACGCCCTC TGCCTGCAGA CCCTAGAGTG GCCAAGGGAC 
     1621 TCGTCCCCAG AGCTGAGGCC AGCACTAGTT CTGCAGCAGT ATCATCATTG ATTGAGAAGT 
     1681 TTGAAAGAGA GCCTGTGATT GTTGCCTCGG ATAGGCCAGC CCCTGGCCCC TGCCCAGTTC 
     1741 CCCCAGAGCC AGCCATGTTG CCACAGCCAC CCCCGCAGCC AACAGGGTCC CAGCTCCCTG 
     1801 AGGGTGAAGC TTCTCGCTGC CTGTTCCTGC TGGCTCCTGG GCCCCGGGAT GGTGAGAAGG 
     1861 TGCCTAACCG GGACAGCGGC ATTGACAGCA TCAGCTCGCC ATCCAACAGT GAAGAGACCT 
     1921 GCTTCGTCAG TGATGATGGG CCCCCCATCC ACAGCCTCTG TCCTGGGCCC CCCGCCCTGG 
     1981 CTAGTATGCC AGTAGCTTTG GCCGACCCCC ACCGGCCTGG CTCCCAGGAG GTTGACAGTG 
     2041 ACCTGGAAGA GGAAGAGGAA GAAGAAGAGG AGGAAAAGGA AAGAGAAATT CCAGTGCCCC 
     2101 CGATGGAGAG ACAGGAGTCT GTGGAGCTGA CTGTGCAGCA GAAGGTGTTC CATATTGCCA 
     2161 ATGAGCTCCT GCAAACTGAG AAGGCCTATG TTTCCAGGCT CCATCTCCTG GATCAGGTAT 
     2221 TCTGTGCCCG GCTGCTGGAA GAAGCTCGGA ACCGCAGTTC CTTCCCCGCA GATGTTGTCC 
     2281 ACGGCATCTT CTCTAACATC TGCTCCATCT ATTGCTTCCA CCAGCAGTTC CTGCTGCCTG 
     2341 AGCTAGAGAA GCGCATGGAG GAATGGGACC GCTACCCACG CATTGGTGAC ATTCTTCAGA 
     2401 AGTTGGCACC CTTCCTCAAG ATGTATGGCG AGTATGTGAA AAACTTCGAC CGGGCGGTAG 
     2461 AGCTGGTCAA CACATGGACA GAGCGGTCTA CCCAGTTTAA AGTCATCATC CATGAAGTAC 
     2521 AGAAGGAGGA AGCCTGTGGC AACCTGACCT TGCAGCACCA CATGCTGGAG CCCGTGCAGC 
     2581 GTATCCCCCG CTATGAACTT CTTCTCAAGG ACTATCTGTT AAAGCTGCCT CATGGCTCCC 
     2641 CGGACAGCAA GGATGCCCAG AAGTCTTTGG AGCTGATAGC CACAGCAGCA GAACACTCTA 
     2701 ATGCTGCCAT CCGGAAAATG GAACGAATGC ACAAGCTGCT GAAGGTGTAT GAGCTGCTAG 
     2761 GGGGTGAGGA GGACATTGTC AGTCCCACCA AAGAGCTCAT AAAGGAAGGC CATATCCTTA 
     2821 AGCTGTCAGC AAAGAATGGG ACCACTCAAG ATCGATACCT CATATTATTC AACGACCGCC 
     2881 TCCTTTACTG CGTGCCCAGG CTGCGGCTCC TTGGTCAGAA GTTTAGCGTG CGGGCACGCA 
     2941 TTGATGTAGA TGGCATGGAG CTAAAGGAGA GCTCCAACCT CAACATGCCT CGGACCTTCC 
     3001 TGGTGTCAGG AAAGCAGCGC TCTCTCGAAC TGCAGGCCAG GACTGAGGAG GAGAAGAAAG 
     3061 ACTGGGTCCA GGCCATCAAT TCCACCCTCC TGAAGCATGA ACAGACCCTG GAGACTTTCA 
     3121 AACTGTTGAA CTCAACAAAC AGGGATGATG AAGATACTCC CCCTAACTCT CCAAACGTGG 
     3181 ATCTTGGGAA GAGGGCACCT ACGCCTATCC GGGAAAAGGA AGTCACCATG TGCATGCGCT 
     3241 GCCAGGAGCC CTTCAATTCC ATCACCAAAC GCAGGCACCA CTGCAAGGCC TGTGGGCATG 
     3301 TGGTTTGTGG GAAATGCTCT GAGTTCCGAG CCCGACTCAT CTATGACAAC AATCGTTCCA 
     3361 ACCGTGTGTG CACTGATTGC TATGTGGCCT TGCATGGAGC ACCTGGGAGC AGTCCAGCCT 
     3421 GTAGCCAGCA TACACCCCAG CGCAGGAGGT CCATCCTAGA GAAACAGGCC TCGGTCGCTG 
     3481 CTGAGAACAG CGTCATCTGC AGCTTCCTGC ACTACATGGA GAAGGGTGGG AAAGGATGGC 
     3541 ACAAGGCATG GTTTGTGGTT CCTGAGAATG AACCCTTGGT GCTGTACATC TATGGAGCCC 
     3601 CTCAGGATGT GAAAGCCCAG CGCAGCCTGC CCCTCATTGG CTTTGAGGTG GGGCCTCCTG 
     3661 AGGCAGGAGA GCGGCCAGAC AGGAGGCATG TCTTCAAGAT CACACAAAGC CACCTAAGCT 
     3721 GGTACTTCAG CCCAGAGACA GAAGAACTCC AGCGGCGCTG GATGGCTGTT CTCGGCAGGG 
     3781 CGGGCCGTGG GGACACATTC TGCCCAGGGC CCACACTGTC TGAGGACAAG GAGATGGAGG 
     3841 AGACACCAGT GGCAGCCTCA GGAGCCACTG CTGAACCTCC TGAAGCCTCC CAGACCCGAG 
     3901 ACAAGACCAC GCGTACGCGG CCGCTCGAGC AGAAACTCAT CTCAGAAGAG GATCTGGCAG 
     3961 CAAATGATAT CCTGGATTAC AAGGATGACG ACGATAAGGT TTAAACGGCC GGCCGCGGTC 
     4021 ATAGCTGTTT CCTGAACAGA TCCCGGGTGG CATCCCTGTG ACCCCTCCCC AGTGCCTCTC 
     4081 CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC ACCAGCCTTG TCCTAATAAA ATTAAGTTGC 
     4141 ATCATTTTGT CTGACTAGGT GTCCTTCTAT AATATTATGG GGTGGAGGGG GGTGGTATGG 
     4201 AGCAAGGGGC AAGTTGGGAA GACAACCTGT AGGGCCTGCG GGGTCTATTG GGAACCAAGC 
     4261 TGGAGTGCAG TGGCACAATC TTGGCTCACT GCAATCTCCG CCTCCTGGGT TCAAGCGATT 
     4321 CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA TTCCAGGCAT GCATGACCAG GCTCAGCTAA 
     4381 TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC ACCATATTGG CCAGGCTGGT CTCCAACTCC 
     4441 TAATCTCAGG TGATCTACCC ACCTTGGCCT CCCAAATTGC TGGGATTACA GGCGTGAACC 
     4501 ACTGCTCCCT TCCCTGTCCT TCTGATTTTA AAATAACTAT ACCAGCAGGA GGACGTCCAG 
     4561 ACACAGCATA GGCTACCTGG CCATGCCCAA CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC 
     4621 ACTGTCCTCT CATGCGTTGG GTCCACTCAG TAGATGCCTG TTGAATTGGG TACGCGGCCA 
     4681 GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA 
     4741 TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC 
     4801 CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG 
     4861 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG 
     4921 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT 
     4981 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT 
     5041 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG 
     5101 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT 
     5161 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT 
     5221 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT 
     5281 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC 
     5341 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC 
     5401 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG 
     5461 TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA 
     5521 AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA CCTGAGGCTA TGGCAGGGCC 
     5581 TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG GCCTTCACCC GAACTTGGGG GGTGGGGTGG 
     5641 GGAAAAGGAA GAAACGCGGG CGTATTGGCC CCAATGGGGT CTCGGTGGGG TATCGACAGA 
     5701 GTGCCAGCCC TGGGACCGAA CCCCGCGTTT ATGAACAAAC GACCCAACAC CGTGCGTTTT 
     5761 ATTCTGTCTT TTTATTGCCG TCATAGCGCG GGTTCCTTCC GGTATTGTCT CCTTCCGTGT 
     5821 TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA GAACTCCAGC ATGAGATCCC CGCGCTGGAG 
     5881 GATCATCCAG CCGGCGTCCC GGAAAACGAT TCCGAAGCCC AACCTTTCAT AGAAGGCGGC 
     5941 GGTGGAATCG AAATCTCGTG ATGGCAGGTT GGGCGTCGCT TGGTCGGTCA TTTCGAACCC 
     6001 CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA AGGCGATAGA AGGCGATGCG CTGCGAATCG 
     6061 GGAGCGGCGA TACCGTAAAG CACGAGGAAG CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA 
     6121 GCAATATCAC GGGTAGCCAA CGCTATGTCC TGATAGCGAT CCGCCACACC CAGCCGGCCA 
     6181 CAGTCGATGA ATCCAGAAAA GCGGCCATTT TCCACCATGA TATTCGGCAA GCAGGCATCG 
     6241 CCATGGGTCA CGACGAGATC CTCGCCGTCG GGCATGCTCG CCTTGAGCCT GGCGAACAGT 
     6301 TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG TCCAGATCAT CCTGATCGAC AAGACCGGCT 
     6361 TCCATCCGAG TACGTGCTCG CTCGATGCGA TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA 
     6421 GCCGGATCAA GCGTATGCAG CCGCCGCATT GCATCAGCCA TGATGGATAC TTTCTCGGCA 
     6481 GGAGCAAGGT GAGATGACAG GAGATCCTGC CCCGGCACTT CGCCCAATAG CAGCCAGTCC 
     6541 CTTCCCGCTT CAGTGACAAC GTCGAGCACA GCTGCGCAAG GAACGCCCGT CGTGGCCAGC 
     6601 CACGATAGCC GCGCTGCCTC GTCTTGCAGT TCATTCAGGG CACCGGACAG GTCGGTCTTG 
     6661 ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC AGCCGGAACA CGGCGGCATC AGAGCAGCCG 
     6721 ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT AGCCTCTCCA CCCAAGCGGC CGGAGAACCT 
     6781 GCGTGCAATC CATCTTGTTC AATCATGCGA AACGATCCTC ATCCTGTCTC TTGATCGATC 
     6841 TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC TCCTCACTAC TTCTGGAATA GCTCAGAGGC 
     6901 CGAGGCGGCC TCGGCCTCTG CATAAATAAA AAAAATTAGT CAGCCATGGG GCGGAGAATG 
     6961 GGCGGAACTG GGCGGAGTTA GGGGCGGGAT GGGCGGAGTT AGGGGCGGGA CTATGGTTGC 
     7021 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     7081 ACACCTGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     7141 TGGGGACTTT CCACACCCTA ACTGACACAC ATTCCACAGC TGGTTCTTTC CGCCTCAGGA 
     7201 CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC 
     7261 ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA 
     7321 GTGCCACCTG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC 
     7381 AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC 
     7441 TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG 
     7501 TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA 
     7561 CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC 
     7621 TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT 
     7681 TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA 
     7741 CAAAAATTTA ACGCGAATTT T
//