LOCUS       MR211296                7749 bp    DNA     circular  SYN 
DEFINITION  Thbs3 (Myc-DDK-tagged) - Mouse thrombospondin 3 (Thbs3)
ACCESSION   NM_013691
VERSION     NM_013691.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3906..3997 
                     /label="Myc-DDK"  
     misc_feature    4027..4612 
                     /label="PolyA signal"  
     misc_feature    4084..4103 
                     /label="Restriction Enzyme"  
     misc_feature    4888..5560 
                     /label="ColE1"  
     misc_feature    5990..6784 
                     /label="Kan/Neo"  
     misc_feature    6819..7121 
                     /label="SV40 ori"  
     misc_feature    7207..7684 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3888..3894 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3888 
                     /label="Thbs3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGAAGCCG GAACTTTGGG GTGTCCTGGC TCTCCTCCTC CTCTGCTCTT 
     1081 ACACGTGTGG CAGTCAGGAC CTTCAGGTAA TTGACCTGCT GACAGTGGGC GAATCCAGGC 
     1141 AGATGGTAGC TGTGGCAGAG AAGATTCGGA CAGCTTTACT CACTGCTGGG GATATCTACC 
     1201 TCTTGTCCAC TTTCCGCCTC CCTCCAAAGC AAGGTGGTGT CCTCTTTGGC CTCTACTCTC 
     1261 GCCAAGACAA CACACGATGG CTGGAGGCCT CTGTTGTGGG CAAGATCAAC AAAGTGCTGG 
     1321 TGCGGTACCA GCGGGAAGAT GGCAAAGTCC ATGCAGTGAA CCTACAGCAA GCAGGCCTGG 
     1381 CTGATGGGCG CACACACACG GCTCTCCTGC GACTCCGAGG ACCTTCTCGA CCCAGCCCTG 
     1441 GCCTGCAGCT GTATGTGGAC TGTAAACTGG GTGACCAACA TGCTGGCCTT CCAGCACTGG 
     1501 CCCCGATCCC TCCAGCAGAG GTCAGTGGAC TGGAGATTAG AACTGGACAA AAGGCTTATC 
     1561 TGAGGATGCA GGGCTTTGTG GAATCGATGA AAATTATTCT GGGTGGATCC ATGGCTCGGG 
     1621 TAGGAGCCCT GAGTGAATGT CCATTCCAAG GAGACGACTC CATTCACAAT GCAGTGACCA 
     1681 GCGCACTGCA ATCCATTCTA GGGGAGCAGA CCAAGGCATT GGTCACCCAG CTCACCCTCT 
     1741 TCAACCAGAT CCTAGTGGAG CTTCGGGACG ACATCCGAGA CCAGGTGAAG GAAATGTCAC 
     1801 TCATCCGGAA CACCATCATG GAGTGTCAGG TGTGCGGTTT CCACGAGCAG CGTTCACACT 
     1861 GCAGCCCCAG CCCCTGCTTC CGAGGCGTGG ACTGTATGGA AGTGTATGAG TACCCGGGCT 
     1921 ATCGCTGTGG GCCCTGCCCA CCTGGCCTGC AGGGCAATGG CACCCACTGT GATGACATCA 
     1981 ACGAGTGTGC TCACGCTGAC CCCTGTTTCC CGGGGTCCAG CTGCATCAAC ACCATGCCTG 
     2041 GCTTCCACTG CGAGGCCTGT CCTCCAGGGT ACAAGGGCAC CCGAGTGTCT GGTGTGGGCA 
     2101 TCGACTATGC TCGAGCCAGC AAACAGGTCT GCAATGACAT TGATGAATGT AATGATGGTA 
     2161 ACAACGGAGG CTGTGACCCA AATTCCATCT GCACCAACAC GGTGGGGTCT TTCAAATGTG 
     2221 GTCCCTGCCG CCTGGGTTTC CTGGGGAACC AGAGCCAGGG CTGCGTTCCA GCAAGGACCT 
     2281 GCCACAGCCC TGCCCACAGC CCCTGCCACA TCCACGCCCA CTGTCTCTTT GAACGCAATG 
     2341 GCGCTGTGTC ATGCCAGTGT AACGTGGGCT GGGCAGGGAA TGGGAACGTG TGTGGGCCTG 
     2401 ACACAGATAT AGACGGCTAT CCGGACCAGG CTCTTCCCTG CATGGATAAC AACAAGCACT 
     2461 GCAAGCAGGA CAATTGCCTT TTGACACCCA ACTCTGGGCA AGAAGATGCT GATAACGATG 
     2521 GTGTCGGGGA CCAGTGTGAT GACGATGCTG ACGGGGATGG TATCAAGAAT GTGGAGGACA 
     2581 ATTGTCGACT GTTCCCCAAC AAGGACCAGC AGAATTCAGA CACAGACTCG TTTGGTGATG 
     2641 CCTGCGACAA CTGTCCCAAT GTTCCCAACA ATGACCAGAA GGACACGGAT GGCAATGGAG 
     2701 AAGGAGATGC CTGTGACAAC GATGTGGACG GGGATGGCAT CCCCAACGGG TTGGACAATT 
     2761 GCCCTAAAGT ACCCAACCCA CTGCAGACAG ACCGGGATGA AGACGGAGTG GGAGATGCTT 
     2821 GCGACAGCTG CCCTGAAATG AGCAATCCTA CCCAGACAGA TGCAGACAGC GACCTGGTGG 
     2881 GGGATGTCTG TGACACCAAC GAAGACAGCG ACGGAGACGG GCACCAGGAC ACCAAGGACA 
     2941 ACTGTCCTCA GCTGCCAAAT AGCTCTCAGC TGGACTCAGA CAATGACGGG CTTGGAGATG 
     3001 AGTGTGACGG GGACGATGAC AATGACGGTG TCCCAGATTA CATTCCTCCC GGGCCTGATA 
     3061 ACTGTCGCCT GGTACCCAAT CCTAATCAGA AGGACTCAGA TGGCAACGGT GTGGGTGATG 
     3121 TCTGTGAAGA TGACTTTGAC AATGACGCAG TGGTTGACCC TCTGGATGTG TGCCCGGAGA 
     3181 GTGCAGAAGT TACCCTCACA GACTTCCGGG CCTATCAGAC TGTGATCCTG GATCCCGAGG 
     3241 GTGACGCCCA GATCGATCCA AACTGGGTAG TGCTCAACCA GGGCATGGAA ATTGTTCAGA 
     3301 CTATGAACAG TGACCCGGGT CTTGCAGTTG GGTACACGGC CTTTAATGGC GTGGACTTTG 
     3361 AAGGCACCTT CCACGTGAAC ACAGTCACTG ATGATGACTA TGCGGGCTTC CTCTTCAGTT 
     3421 ACCAGGACAG TGGCCGCTTC TATGTGGTCA TGTGGAAACA GACGGAGCAG ACCTACTGGC 
     3481 AGGCCACGCC TTTCCGAGCA GTGGCCCAAC CAGGGCTTCA GCTCAAGGCA GTGACATCCA 
     3541 TATCTGGCCC AGGTGAGCAC CTCCGGAATG CTCTGTGGCA TACTGGCCAC ACCCCTGATC 
     3601 AGGTACGACT GCTGTGGACT GACCCACGGA ATGTGGGTTG GCGAGACAAG ACATCCTATA 
     3661 GATGGCGGCT GCTCCACCGG CCCCAAGTTG GCTACATTCG GGTGAAGCTC TATGAGGGTC 
     3721 CCCAGCTAGT GGCGGATTCT GGGGTGATTA TTGACACGTC CATGCGAGGG GGGCGTCTCG 
     3781 GCGTATTCTG CTTCTCCCAA GAAAACATCA TTTGGTCCAA TCTCCAGTAC CGATGCAATG 
     3841 ACACAGTGCC TGAGGACTTT GAGCCATTCC GGAGGCAGCT GCTCCAGGGA AGAGTGACGC 
     3901 GTACGCGGCC GCTCGAGCAG AAACTCATCT CAGAAGAGGA TCTGGCAGCA AATGATATCC 
     3961 TGGATTACAA GGATGACGAC GATAAGGTTT AAACGGCCGG CCGCGGTCAT AGCTGTTTCC 
     4021 TGAACAGATC CCGGGTGGCA TCCCTGTGAC CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA 
     4081 GTTGCCACTC CAGTGCCCAC CAGCCTTGTC CTAATAAAAT TAAGTTGCAT CATTTTGTCT 
     4141 GACTAGGTGT CCTTCTATAA TATTATGGGG TGGAGGGGGG TGGTATGGAG CAAGGGGCAA 
     4201 GTTGGGAAGA CAACCTGTAG GGCCTGCGGG GTCTATTGGG AACCAAGCTG GAGTGCAGTG 
     4261 GCACAATCTT GGCTCACTGC AATCTCCGCC TCCTGGGTTC AAGCGATTCT CCTGCCTCAG 
     4321 CCTCCCGAGT TGTTGGGATT CCAGGCATGC ATGACCAGGC TCAGCTAATT TTTGTTTTTT 
     4381 TGGTAGAGAC GGGGTTTCAC CATATTGGCC AGGCTGGTCT CCAACTCCTA ATCTCAGGTG 
     4441 ATCTACCCAC CTTGGCCTCC CAAATTGCTG GGATTACAGG CGTGAACCAC TGCTCCCTTC 
     4501 CCTGTCCTTC TGATTTTAAA ATAACTATAC CAGCAGGAGG ACGTCCAGAC ACAGCATAGG 
     4561 CTACCTGGCC ATGCCCAACC GGTGGGACAT TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA 
     4621 TGCGTTGGGT CCACTCAGTA GATGCCTGTT GAATTGGGTA CGCGGCCAGC GGCGAGCGGT 
     4681 ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA 
     4741 GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC 
     4801 GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG 
     4861 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT 
     4921 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG 
     4981 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG 
     5041 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG 
     5101 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC 
     5161 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG 
     5221 GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT 
     5281 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG 
     5341 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC 
     5401 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT 
     5461 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT 
     5521 TAAATCAATC TAAAGTATAT ATGAGTAACC TGAGGCTATG GCAGGGCCTG CCGCCCCGAC 
     5581 GTTGGCTGCG AGCCCTGGGC CTTCACCCGA ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA 
     5641 AACGCGGGCG TATTGGCCCC AATGGGGTCT CGGTGGGGTA TCGACAGAGT GCCAGCCCTG 
     5701 GGACCGAACC CCGCGTTTAT GAACAAACGA CCCAACACCG TGCGTTTTAT TCTGTCTTTT 
     5761 TATTGCCGTC ATAGCGCGGG TTCCTTCCGG TATTGTCTCC TTCCGTGTTT CAGTTAGCCT 
     5821 CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT GAGATCCCCG CGCTGGAGGA TCATCCAGCC 
     5881 GGCGTCCCGG AAAACGATTC CGAAGCCCAA CCTTTCATAG AAGGCGGCGG TGGAATCGAA 
     5941 ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG GTCGGTCATT TCGAACCCCA GAGTCCCGCT 
     6001 CAGAAGAACT CGTCAAGAAG GCGATAGAAG GCGATGCGCT GCGAATCGGG AGCGGCGATA 
     6061 CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT TCGCCGCCAA GCTCTTCAGC AATATCACGG 
     6121 GTAGCCAACG CTATGTCCTG ATAGCGATCC GCCACACCCA GCCGGCCACA GTCGATGAAT 
     6181 CCAGAAAAGC GGCCATTTTC CACCATGATA TTCGGCAAGC AGGCATCGCC ATGGGTCACG 
     6241 ACGAGATCCT CGCCGTCGGG CATGCTCGCC TTGAGCCTGG CGAACAGTTC GGCTGGCGCG 
     6301 AGCCCCTGAT GCTCTTCGTC CAGATCATCC TGATCGACAA GACCGGCTTC CATCCGAGTA 
     6361 CGTGCTCGCT CGATGCGATG TTTCGCTTGG TGGTCGAATG GGCAGGTAGC CGGATCAAGC 
     6421 GTATGCAGCC GCCGCATTGC ATCAGCCATG ATGGATACTT TCTCGGCAGG AGCAAGGTGA 
     6481 GATGACAGGA GATCCTGCCC CGGCACTTCG CCCAATAGCA GCCAGTCCCT TCCCGCTTCA 
     6541 GTGACAACGT CGAGCACAGC TGCGCAAGGA ACGCCCGTCG TGGCCAGCCA CGATAGCCGC 
     6601 GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA CCGGACAGGT CGGTCTTGAC AAAAAGAACC 
     6661 GGGCGCCCCT GCGCTGACAG CCGGAACACG GCGGCATCAG AGCAGCCGAT TGTCTGTTGT 
     6721 GCCCAGTCAT AGCCGAATAG CCTCTCCACC CAAGCGGCCG GAGAACCTGC GTGCAATCCA 
     6781 TCTTGTTCAA TCATGCGAAA CGATCCTCAT CCTGTCTCTT GATCGATCTT TGCAAAAGCC 
     6841 TAGGCCTCCA AAAAAGCCTC CTCACTACTT CTGGAATAGC TCAGAGGCCG AGGCGGCCTC 
     6901 GGCCTCTGCA TAAATAAAAA AAATTAGTCA GCCATGGGGC GGAGAATGGG CGGAACTGGG 
     6961 CGGAGTTAGG GGCGGGATGG GCGGAGTTAG GGGCGGGACT ATGGTTGCTG ACTAATTGAG 
     7021 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCTGGTTGC 
     7081 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     7141 ACACCCTAAC TGACACACAT TCCACAGCTG GTTCTTTCCG CCTCAGGACT CTTCCTTTTT 
     7201 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT 
     7261 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC 
     7321 GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT 
     7381 ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG 
     7441 TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT 
     7501 GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA 
     7561 TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA 
     7621 CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA 
     7681 GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC 
     7741 GCGAATTTT
//