LOCUS       MR211294                7740 bp    DNA     circular  SYN 
DEFINITION  Copb1 (Myc-DDK-tagged) - Mouse coatomer protein complex, subunit beta 1 (Copb1)
ACCESSION   NM_033370
VERSION     NM_033370.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3897..3988 
                     /label="Myc-DDK"  
     misc_feature    4018..4603 
                     /label="PolyA signal"  
     misc_feature    4075..4094 
                     /label="Restriction Enzyme"  
     misc_feature    4879..5551 
                     /label="ColE1"  
     misc_feature    5981..6775 
                     /label="Kan/Neo"  
     misc_feature    6810..7112 
                     /label="SV40 ori"  
     misc_feature    7198..7675 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3879..3885 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3879 
                     /label="Copb1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GACGGCAGCC GAGAACGTGT GCTATACGTT AATTAACGTG CCAATGGATT 
     1081 CAGAACCCCC TTCGGAAATC AGCTTAAAAA ATGACCTGGA GAAAGGCGAT GTGAAGTCAA 
     1141 AGACTGAAGC TTTGAAGAAA GTGATCATTA TGATTCTGAA TGGGGAAAAG CTTCCCGGAC 
     1201 TCCTGATGAC AATCATTCGC TTCGTGCTGC CTCTTCAGGA TCACACCATC AAAAAGTTGC 
     1261 TCCTGGTCTT CTGGGAGATT GTTCCTAAAA CAACTCCAGA TGGAAGGCTC TTACATGAAA 
     1321 TGATTCTTGT GTGTGATGCA TACAGAAAGG ATCTCCAGCA CCCCAATGAG TTTATTCGTG 
     1381 GATCTACTCT TCGTTTCCTT TGCAAATTGA AGGAAGCAGA ATTGCTGGAA CCTTTAATGC 
     1441 CTGCTATCCG TGCGTGCTTG GAACATCGTC ACAGCTATGT TAGGAGAAAT GCTGTTTTGG 
     1501 CCATCTATAC CATCTACAGA AATTTTGAAC ATCTTATACC TGATGCTCCT GAACTGATAC 
     1561 ATGATTTTCT TGTGAATGAG AAGGATGCAA GCTGCAAAAG AAATGCATTT ATGATGCTAA 
     1621 TTCACGCAGA TCAGGATCGA GCTTTGGATT ATTTAAGTAC ATGTATTGAT CAAGTTCAGA 
     1681 CATTTGGAGA CATTCTACAG TTGGTTATTG TTGAACTAAT TTATAAGGTC TGTCATGCTA 
     1741 ATCCATCAGA AAGGGCCCGC TTCATTCGCT GTATCTATAA CTTACTGCAG TCATCTAGTC 
     1801 CTGCTGTAAA ATATGAGGCT GCTGGGACAC TGGTAACACT GTCAAGTGCA CCAACTGCAA 
     1861 TAAAGGCTGC TGCTCAGTGT TACATTGATT TAATTATTAA GGAGAGTGAT AACAATGTAA 
     1921 AGCTCATCGT CCTGGACCGC TTGGTGGAAT TAAAAGAGCA TCCTGCTCAT GAACGAGTCC 
     1981 TGCAGGATCT GGTCATGGAC ATCCTAAGAG TACTGAGTAC GCCAGACTTA GAAGTGCGTA 
     2041 AGAAAACGCT GCAGTTAGCA CTGGATCTTG TCTCATCTAG GAATGTTGAA GAGTTGGTTA 
     2101 TTGTCTTAAA GAAGGAAGTA ATTAAAACAA ATAATGTGTC TGAGCATGAA GACACTGACA 
     2161 AATACCGACA GCTTCTCGTG CGAACACTGC ATTCCTGCTC TGTCCGATTT CCAGATATGG 
     2221 CTGCAAATGT TATTCCTGTG TTAATGGAAT TTCTCAGTGA CAGTAATGAA GCAGCAGCTG 
     2281 CTGATGTCTT AGAGTTTGTT CGTGAAGCCA TTCAGCGCTT TGACAACCTG AGAATGCTGA 
     2341 TTGTTGAGAA GATGCTGGAA GTCTTCCATG CTATTAAGTC TGTCAAGATT TACCGAGGAG 
     2401 CATTGTGGAT CCTGGGAGAG TACTGCAGTA CAAAGGAAGA CATTCAGAGT GTGATGACTG 
     2461 AGGTCCGCAG GTCCCTTGGG GAGATACCAA TTGTAGAATC AGAAATAAAG AAAGAAGCTG 
     2521 GCGAGTTAAA ACCAGAAGAA GAAATAACTG TTGGGCCCGT TCAGAAATTG GTTACGGAGA 
     2581 TGGGCACCTA TGCTACTCAG AGTGCGCTCA GCAGTTCCAG ACCTACCAAG AAAGAAGAAG 
     2641 ACAGACCACC CTTGAGAGGG TTCCTCCTGG ATGGAGATTT CTTTGTTGCT GCTTCCCTCG 
     2701 CCACAACTCT AACCAAGATT GCATTGCGCT ATGTAGCGTT GGTTCAGGAG AAGAAAAAGC 
     2761 AAAATTCTTT TGTTGCTGAG GCTATGTTGC TTATGGCAAC TATCCTTCAT TTGGGAAAAT 
     2821 CCTCTCTTCC TAAGAAGCCA ATTACTGATG ATGATGTAGA TCGAATTTCC CTGTGCCTCA 
     2881 AGGTCTTATC TGAATGCTCA CCTTTAATGA ATGACATCTT TAATAAGGAG TGCAGACAGT 
     2941 CTCTTTCCCA AATGTTATCT GCCAAACTCG AAGAAGAGAA ACTATCCCAG AAGAAAGAAT 
     3001 CTGAAAAGAG AAATGTGACA GTACAGCCTG ATGACCCCAT TTCCTTCATG CAACTAACTG 
     3061 CGAAGAATGA AATGAACTGC AAGGAAGATC AGTTCCAGCT GAGTTTGTTG GCAGCAATGG 
     3121 GTAATACTCA GAGGAAAGAG GCAGCTGATC CCCTAGCATC CAAACTTAAC AAGGTCACTC 
     3181 AGTTGACAGG CTTCTCGGAT CCAGTGTATG CAGAAGCCTA TGTTCATGTC AATCAGTACG 
     3241 ACATCGTCCT GGATGTTCTG GTTGTAAACC AAACCAGTGA TACTTTGCAG AACTGCACAT 
     3301 TAGAGTTAGC CACTCTAGGG GATCTGAAAC TTGTGGAAAA ACCATCTCCT TTGACTCTTG 
     3361 CTCCTCATGA CTTTGCAAAT ATTAAAGCTA ATGTCAAGGT AGCATCAACA GAAAACGGAA 
     3421 TAATTTTTGG CAATATAGTT TATGATGTTT CTGGAGCAGC AAGTGACAGA AACTGTGTGG 
     3481 TCCTCAGTGA CATCCACATT GACATCATGG ACTATATCCA GCCTGCAACT TGCACTGATG 
     3541 CTGAGTTCCG TCAGATGTGG GCCGAATTTG AATGGGAAAA TAAAGTGACA GTTAACACCA 
     3601 ACATGACTGA CCTGAATGAC TACTTACAGC ACATCCTCAA GTCGACCAAC ATGAAGTGCC 
     3661 TGACTCCAGA GAAGGCCCTT TCTGGATACT GTGGCTTTAT GGCAGCCAAT CTTTATGCAC 
     3721 GTTCTATATT TGGAGAGGAT GCACTTGCAA ATGTCAGCAT TGAGAAGCCA GTTCACCAGG 
     3781 GACCAGATGC TGCCGTTACT GGCCATATAA GAATTCGTGC AAAAAGTCAG GGAATGGCCT 
     3841 TGAGCCTTGG AGATAAAATC AACCTGTCTC AAAAGAAGAC TAGTCTAACG CGTACGCGGC 
     3901 CGCTCGAGCA GAAACTCATC TCAGAAGAGG ATCTGGCAGC AAATGATATC CTGGATTACA 
     3961 AGGATGACGA CGATAAGGTT TAAACGGCCG GCCGCGGTCA TAGCTGTTTC CTGAACAGAT 
     4021 CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA GTGCCTCTCC TGGCCCTGGA AGTTGCCACT 
     4081 CCAGTGCCCA CCAGCCTTGT CCTAATAAAA TTAAGTTGCA TCATTTTGTC TGACTAGGTG 
     4141 TCCTTCTATA ATATTATGGG GTGGAGGGGG GTGGTATGGA GCAAGGGGCA AGTTGGGAAG 
     4201 ACAACCTGTA GGGCCTGCGG GGTCTATTGG GAACCAAGCT GGAGTGCAGT GGCACAATCT 
     4261 TGGCTCACTG CAATCTCCGC CTCCTGGGTT CAAGCGATTC TCCTGCCTCA GCCTCCCGAG 
     4321 TTGTTGGGAT TCCAGGCATG CATGACCAGG CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA 
     4381 CGGGGTTTCA CCATATTGGC CAGGCTGGTC TCCAACTCCT AATCTCAGGT GATCTACCCA 
     4441 CCTTGGCCTC CCAAATTGCT GGGATTACAG GCGTGAACCA CTGCTCCCTT CCCTGTCCTT 
     4501 CTGATTTTAA AATAACTATA CCAGCAGGAG GACGTCCAGA CACAGCATAG GCTACCTGGC 
     4561 CATGCCCAAC CGGTGGGACA TTTGAGTTGC TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG 
     4621 TCCACTCAGT AGATGCCTGT TGAATTGGGT ACGCGGCCAG CGGCGAGCGG TATCAGCTCA 
     4681 CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG 
     4741 AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA 
     4801 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA 
     4861 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC 
     4921 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC 
     4981 GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT 
     5041 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG 
     5101 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG 
     5161 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA 
     5221 CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG 
     5281 AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT 
     5341 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT 
     5401 TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG 
     5461 ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT 
     5521 CTAAAGTATA TATGAGTAAC CTGAGGCTAT GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC 
     5581 GAGCCCTGGG CCTTCACCCG AACTTGGGGG GTGGGGTGGG GAAAAGGAAG AAACGCGGGC 
     5641 GTATTGGCCC CAATGGGGTC TCGGTGGGGT ATCGACAGAG TGCCAGCCCT GGGACCGAAC 
     5701 CCCGCGTTTA TGAACAAACG ACCCAACACC GTGCGTTTTA TTCTGTCTTT TTATTGCCGT 
     5761 CATAGCGCGG GTTCCTTCCG GTATTGTCTC CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG 
     5821 GTGGGCGAAG AACTCCAGCA TGAGATCCCC GCGCTGGAGG ATCATCCAGC CGGCGTCCCG 
     5881 GAAAACGATT CCGAAGCCCA ACCTTTCATA GAAGGCGGCG GTGGAATCGA AATCTCGTGA 
     5941 TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT TTCGAACCCC AGAGTCCCGC TCAGAAGAAC 
     6001 TCGTCAAGAA GGCGATAGAA GGCGATGCGC TGCGAATCGG GAGCGGCGAT ACCGTAAAGC 
     6061 ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA AGCTCTTCAG CAATATCACG GGTAGCCAAC 
     6121 GCTATGTCCT GATAGCGATC CGCCACACCC AGCCGGCCAC AGTCGATGAA TCCAGAAAAG 
     6181 CGGCCATTTT CCACCATGAT ATTCGGCAAG CAGGCATCGC CATGGGTCAC GACGAGATCC 
     6241 TCGCCGTCGG GCATGCTCGC CTTGAGCCTG GCGAACAGTT CGGCTGGCGC GAGCCCCTGA 
     6301 TGCTCTTCGT CCAGATCATC CTGATCGACA AGACCGGCTT CCATCCGAGT ACGTGCTCGC 
     6361 TCGATGCGAT GTTTCGCTTG GTGGTCGAAT GGGCAGGTAG CCGGATCAAG CGTATGCAGC 
     6421 CGCCGCATTG CATCAGCCAT GATGGATACT TTCTCGGCAG GAGCAAGGTG AGATGACAGG 
     6481 AGATCCTGCC CCGGCACTTC GCCCAATAGC AGCCAGTCCC TTCCCGCTTC AGTGACAACG 
     6541 TCGAGCACAG CTGCGCAAGG AACGCCCGTC GTGGCCAGCC ACGATAGCCG CGCTGCCTCG 
     6601 TCTTGCAGTT CATTCAGGGC ACCGGACAGG TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC 
     6661 TGCGCTGACA GCCGGAACAC GGCGGCATCA GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA 
     6721 TAGCCGAATA GCCTCTCCAC CCAAGCGGCC GGAGAACCTG CGTGCAATCC ATCTTGTTCA 
     6781 ATCATGCGAA ACGATCCTCA TCCTGTCTCT TGATCGATCT TTGCAAAAGC CTAGGCCTCC 
     6841 AAAAAAGCCT CCTCACTACT TCTGGAATAG CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC 
     6901 ATAAATAAAA AAAATTAGTC AGCCATGGGG CGGAGAATGG GCGGAACTGG GCGGAGTTAG 
     6961 GGGCGGGATG GGCGGAGTTA GGGGCGGGAC TATGGTTGCT GACTAATTGA GATGCATGCT 
     7021 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCTGGTTG CTGACTAATT 
     7081 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCCTAA 
     7141 CTGACACACA TTCCACAGCT GGTTCTTTCC GCCTCAGGAC TCTTCCTTTT TCAATATTAT 
     7201 TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA 
     7261 AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA CGCGCCCTGT 
     7321 AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC 
     7381 AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC 
     7441 TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG 
     7501 CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA 
     7561 TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC 
     7621 CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT TTGATTTATA AGGGATTTTG 
     7681 CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//