LOCUS       MR211256                7704 bp    DNA     circular  SYN 
DEFINITION  Vps11 (Myc-DDK-tagged) - Mouse vacuolar protein sorting 11 (yeast) (Vps11)
ACCESSION   NM_027889
VERSION     NM_027889.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3861..3952 
                     /label="Myc-DDK"  
     misc_feature    3982..4567 
                     /label="PolyA signal"  
     misc_feature    4039..4058 
                     /label="Restriction Enzyme"  
     misc_feature    4843..5515 
                     /label="ColE1"  
     misc_feature    5945..6739 
                     /label="Kan/Neo"  
     misc_feature    6774..7076 
                     /label="SV40 ori"  
     misc_feature    7162..7639 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3843..3849 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3843 
                     /label="Vps11"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGGCCTAC CTGCAGTGGC GGCGTTTCGT TTTCTTCGAG AAGGAGCTGG 
     1081 TGAAGGAGCC GCTGGGTAAT GATGGGGCTG CTCCCGGTGC CGCGCCTGTG TCGGGATCCG 
     1141 CTGCTTCCAA GTTCCTTTGC CTCCCTCCTG GCATCACTGT CTGCGACTCA GGCCGCGGGA 
     1201 GCCTGGTCTT TGGAGATATG GAAGGCCAGA TCTGGTTCTT GCCACGTTCT CTACAGCTTA 
     1261 CAGGGTTCCA GGCCTACAAA CTACGGGTGA CACACTTATA TCAGCTGAAG CAGCACAATA 
     1321 TTCTGGCATC GGTTGGTGAG GATGAAGAGG GTATTAACCC TCTGGTAAAG ATCTGGAACC 
     1381 TGGAAAAGAG AGACGGTGGC AATCCACTCT GCACCCGGAT TTTCCCTGCC ATCCCAGGGA 
     1441 CAGAGCCAAC TGTTGTGTCT TGTTTGACGG TCCATGAGAA TCTCAACTTT ATGGCCATTG 
     1501 GGTTCACAGA TGGCAGTGTC ACCCTGAACA AGGGAGATAT TACCCGGGAC CGGCACAGCA 
     1561 AGACGCAGAT TTTGCACAAG GGCAACTATC CCGTTACTGG GCTGGCCTTT CGCCAAGCAG 
     1621 GAAAGACCAC CCACTTGTTT GTCGTGACAA CTGAAAATGT TCAGTCTTAT ATAGTTTCTG 
     1681 GAAAGGACTA CCCTCGTGTG GAGTTGGACA CCCATGGTTG TGGCCTGCGC TGTTCAGCCC 
     1741 TGAGCGACCC TTCTCAAGAC CTGCAGTTCA TTGTTGCTGG GGATGAGTGT GTCTACCTTT 
     1801 ACCAGCCTGA TGAACGTGGA CCCTGCTTTG CCTTTGAGGG CCATAAGCTC ATCGTGCACT 
     1861 GGTTTAGAGG CTACCTTGTC ATTGTCTCCC GTGACCGGAA AGTTTCTCCC AAGTCCGAGT 
     1921 TTACCAGCAG GGACTCACAG AACTCCGACA AGCAGATTCT CAACATCTAT GACCTGTGCA 
     1981 ACAAGTTCAT AGCCTACAGC GCTGGGTTTG AGGATATAGT GGATGTGCTG GCTGAGTGGG 
     2041 GCTCCTTGTA CGTGCTGACG CGGGATGGGC GGGTCCACGC ATTGCAGGAG AAGGACACAC 
     2101 AGACCAAACT GGAGATGCTG TTTAAGAAGA ATCTATTTGA GATGGCAATT AATCTGGCTA 
     2161 AGAGTCAACA TTTGGACAGT GATGGGTTGG CTCAAATCTT CATGCAGTAT GGAGACCATC 
     2221 TCTACAGCAA GGGCAACCAT GATGGGGCTG TGCAGCAGTA TATCCGAACC ATTGGAAAGT 
     2281 TGGAGCCATC CTATGTGATC CGGAAGTTTC TGGATGCCCA GCGCATCCAC AACTTGACCG 
     2341 CCTACCTGCA AACCCTGCAT CGGCAGTCTC TGGCTAATGC TGATCATACC ACCCTGCTTC 
     2401 TGAACTGCTA TACCAAGCTC AAGGACAGCT CGAAGCTGGA GGAATTCATC AAGACAAAGA 
     2461 GTGAGAGTGA AGTCCACTTT GATGTAGAGA CAGCCATCAA GGTCCTCCGG CAGGCTGGCT 
     2521 ACTACTCCCA TGCCCTCTAT CTGGCTGAGA ATCATGCACA TCATGAGTGG TACTTGAAGA 
     2581 TCCAGCTAGA GGACATTAAG AACTATCAGG AAGCCCTTCG GTATATCGGG AAGCTGCCTT 
     2641 TCGAGCAAGC GGAGAGTAAC ATGAAACGCT ATGGCAAGAC CCTCATGCAC CACATACCGG 
     2701 AGCAGACAAC CCAGTTGCTG AAAGGCCTCT GCACTGACTA TCGACCCAGT CTGGAAGGCC 
     2761 GAGGCGATCG GGAGGCTCTG AGCTGCCGGG CCAGTTCTGA GGAGTTCATC CCCATCTTTG 
     2821 CCAACAACCC ACGAGAGCTG AAAGCCTTCC TAGAACACAT GAGTGAGGTG CAGCCGGACT 
     2881 CACCGCAGGG CATCTATGAC ACGCTCCTGG AGCTGCGGCT GCAGAACTGG GCCCATGAGA 
     2941 AAGATCCCCA GGCCAAGGAG AAACTTCATG CAGAGGCCAT CTCCCTGCTG AAGAGTGGCC 
     3001 GATTCTGTGA CGTGTTTGAC AAGGCATTGG TTCTGTGCCA GATGCACGAC TTCCAGGATG 
     3061 GGGTCCTTTA CCTCTATGAG CAGGGGAAGC TGTTCCAGCA GATCATGCAT TACCACATGC 
     3121 AGCACGAGCA GTACCGGCAG GTGATTGCCG TGTGTGAGCG CCATGGAGAG CAGGAGCCCT 
     3181 CCCTGTGGGA ACAGGCCCTC AGCTACTTCG CCCGCAAGGA GGAGGACTGC AAGGAGTATG 
     3241 TGGCGGCTGT GCTCAGGCAC ATTGAGAACA AGAGCCTCAT GCCGCCTCTT CTAGTGGTGC 
     3301 AGACCCTGGC CCACAACTCC ACAGCTACCC TGTCTATCAT CCGAGATTAC CTGGTCCAAA 
     3361 AACTGCAGAA ACAGAGCCAG CAGATTGCAC AGGATGAGCT CCGGGTGCGG CGATACAGAG 
     3421 AGGAGACCAC TCGCATCCGC CAGGAAATCC AGGAGCTCAA GGCGAGTCCA AAGATTTTCC 
     3481 AGAAGACCAA GTGTAGCATC TGTAACAGTG CCTTGGAGCT GCCCTCAGTC CACTTCCTGT 
     3541 GTGGCCACTC CTTCCACCAA CACTGCTTTG AGAGTTACTC AGAAAGTGAT GCTGACTGCC 
     3601 CCACCTGCCT CCCTGAGAAC CGCAAGGTGA TGGATATGAT CCGGGCCCAG GAACAGAAAC 
     3661 GAGATCTCCA CGATCAGTTC CAACATCAGC TCAAGTGCTC GAATGACAGC TTCTCTGTGA 
     3721 TTGCGGACTA CTTTGGCCGA GGGGTTTTCA ACAAGTTGAC TCTGCTGACC GACCCTCCTA 
     3781 CAGCCAGACT GACCCCCAGC CTGGAGGCTG GACTGCAGCG GGACCTGCTG ATGCACTCCA 
     3841 GGAGGGGAAC TACGCGTACG CGGCCGCTCG AGCAGAAACT CATCTCAGAA GAGGATCTGG 
     3901 CAGCAAATGA TATCCTGGAT TACAAGGATG ACGACGATAA GGTTTAAACG GCCGGCCGCG 
     3961 GTCATAGCTG TTTCCTGAAC AGATCCCGGG TGGCATCCCT GTGACCCCTC CCCAGTGCCT 
     4021 CTCCTGGCCC TGGAAGTTGC CACTCCAGTG CCCACCAGCC TTGTCCTAAT AAAATTAAGT 
     4081 TGCATCATTT TGTCTGACTA GGTGTCCTTC TATAATATTA TGGGGTGGAG GGGGGTGGTA 
     4141 TGGAGCAAGG GGCAAGTTGG GAAGACAACC TGTAGGGCCT GCGGGGTCTA TTGGGAACCA 
     4201 AGCTGGAGTG CAGTGGCACA ATCTTGGCTC ACTGCAATCT CCGCCTCCTG GGTTCAAGCG 
     4261 ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG GGATTCCAGG CATGCATGAC CAGGCTCAGC 
     4321 TAATTTTTGT TTTTTTGGTA GAGACGGGGT TTCACCATAT TGGCCAGGCT GGTCTCCAAC 
     4381 TCCTAATCTC AGGTGATCTA CCCACCTTGG CCTCCCAAAT TGCTGGGATT ACAGGCGTGA 
     4441 ACCACTGCTC CCTTCCCTGT CCTTCTGATT TTAAAATAAC TATACCAGCA GGAGGACGTC 
     4501 CAGACACAGC ATAGGCTACC TGGCCATGCC CAACCGGTGG GACATTTGAG TTGCTTGCTT 
     4561 GGCACTGTCC TCTCATGCGT TGGGTCCACT CAGTAGATGC CTGTTGAATT GGGTACGCGG 
     4621 CCAGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG 
     4681 GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA 
     4741 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG 
     4801 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC 
     4861 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC 
     4921 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC 
     4981 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG 
     5041 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC 
     5101 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA 
     5161 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC 
     5221 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC 
     5281 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG 
     5341 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC 
     5401 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA 
     5461 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAACCTGAGG CTATGGCAGG 
     5521 GCCTGCCGCC CCGACGTTGG CTGCGAGCCC TGGGCCTTCA CCCGAACTTG GGGGGTGGGG 
     5581 TGGGGAAAAG GAAGAAACGC GGGCGTATTG GCCCCAATGG GGTCTCGGTG GGGTATCGAC 
     5641 AGAGTGCCAG CCCTGGGACC GAACCCCGCG TTTATGAACA AACGACCCAA CACCGTGCGT 
     5701 TTTATTCTGT CTTTTTATTG CCGTCATAGC GCGGGTTCCT TCCGGTATTG TCTCCTTCCG 
     5761 TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC GAAGAACTCC AGCATGAGAT CCCCGCGCTG 
     5821 GAGGATCATC CAGCCGGCGT CCCGGAAAAC GATTCCGAAG CCCAACCTTT CATAGAAGGC 
     5881 GGCGGTGGAA TCGAAATCTC GTGATGGCAG GTTGGGCGTC GCTTGGTCGG TCATTTCGAA 
     5941 CCCCAGAGTC CCGCTCAGAA GAACTCGTCA AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA 
     6001 TCGGGAGCGG CGATACCGTA AAGCACGAGG AAGCGGTCAG CCCATTCGCC GCCAAGCTCT 
     6061 TCAGCAATAT CACGGGTAGC CAACGCTATG TCCTGATAGC GATCCGCCAC ACCCAGCCGG 
     6121 CCACAGTCGA TGAATCCAGA AAAGCGGCCA TTTTCCACCA TGATATTCGG CAAGCAGGCA 
     6181 TCGCCATGGG TCACGACGAG ATCCTCGCCG TCGGGCATGC TCGCCTTGAG CCTGGCGAAC 
     6241 AGTTCGGCTG GCGCGAGCCC CTGATGCTCT TCGTCCAGAT CATCCTGATC GACAAGACCG 
     6301 GCTTCCATCC GAGTACGTGC TCGCTCGATG CGATGTTTCG CTTGGTGGTC GAATGGGCAG 
     6361 GTAGCCGGAT CAAGCGTATG CAGCCGCCGC ATTGCATCAG CCATGATGGA TACTTTCTCG 
     6421 GCAGGAGCAA GGTGAGATGA CAGGAGATCC TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG 
     6481 TCCCTTCCCG CTTCAGTGAC AACGTCGAGC ACAGCTGCGC AAGGAACGCC CGTCGTGGCC 
     6541 AGCCACGATA GCCGCGCTGC CTCGTCTTGC AGTTCATTCA GGGCACCGGA CAGGTCGGTC 
     6601 TTGACAAAAA GAACCGGGCG CCCCTGCGCT GACAGCCGGA ACACGGCGGC ATCAGAGCAG 
     6661 CCGATTGTCT GTTGTGCCCA GTCATAGCCG AATAGCCTCT CCACCCAAGC GGCCGGAGAA 
     6721 CCTGCGTGCA ATCCATCTTG TTCAATCATG CGAAACGATC CTCATCCTGT CTCTTGATCG 
     6781 ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA GCCTCCTCAC TACTTCTGGA ATAGCTCAGA 
     6841 GGCCGAGGCG GCCTCGGCCT CTGCATAAAT AAAAAAAATT AGTCAGCCAT GGGGCGGAGA 
     6901 ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG GATGGGCGGA GTTAGGGGCG GGACTATGGT 
     6961 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     7021 TCCACACCTG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     7081 GCCTGGGGAC TTTCCACACC CTAACTGACA CACATTCCAC AGCTGGTTCT TTCCGCCTCA 
     7141 GGACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA 
     7201 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA 
     7261 AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG 
     7321 CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT 
     7381 TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA 
     7441 GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT 
     7501 TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG 
     7561 TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT 
     7621 TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT 
     7681 TAACAAAAAT TTAACGCGAA TTTT
//